Abstract:
BACKGROUND:In this work we studied the relationship between the enhanced expression of DR5 receptor and the effect of combination of TRAIL and ionizing radiation on cell cycle arrest and apoptosis induction in human leukemia cell line HL-60. MATERIAL AND METHODS:DR5, APO2.7 and cell cycle were analyzed by flow cytometry. Proteins Bid and Mcl-1 were analyzed by Western-blotting. For clonogenic survival, colony assay on methylcellulose was used. RESULTS:Ionizing radiation caused significantly enhanced positivity of DR5 receptors 24 h after irradiation with high doses (6 and 8 Gy). An increase of DR5 receptor positivity after a dose of 2 Gy was not statistically significant and application of TRAIL 48 h after irradiation did not increase the apoptosis induction. However, a decrease of radiation-induced G(2) phase arrest and an increase of apoptosis were observed when TRAIL was applied 16 h before irradiation with the dose of 2 Gy. Incubation with 6 microg/l TRAIL for 16 h reduced D(0) value from 2.9 Gy to 1.5 Gy. The induction of apoptosis by TRAIL was accompanied by Bid cleavage and a decrease of antiapoptotic Mcl-1 16 h after incubation with TRAIL. CONCLUSION:TRAIL in concentration of 6 microg/l applied 16 h before irradiation by the dose of 1.5 Gy caused the death of 63% of clonogenic tumor cells, similarly as the dose of 2.9 Gy alone, which is in good correlation with the enhanced apoptosis induction.
journal_name
Acta Biochim Poljournal_title
Acta biochimica Polonicaauthors
Rezácová M,Vávrová J,Vokurková D,Tichý A,Knízek J,Psutka Jsubject
Has Abstractpub_date
2005-01-01 00:00:00pages
889-95issue
4eissn
0001-527Xissn
1734-154Xpii
20051032journal_volume
52pub_type
杂志文章abstract::1. The everted-sac technique has been used to study the time-dependent effect of low-calcium diet on calcium active transport along rat small intestine. 2. In animals maintained on standard diet active translocation of calcium was limited to proximal 10 cm of the intestine. 3. In response to calcium restriction, calci...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1976-01-01 00:00:00
abstract::DNA methylation and histone modifications are major components of cellular epigenetic pattern determining gene expression. Cancer cells have their own epigenetic array, which can be different in cells of primary and metastatic tumors. In the present work we investigated effects of 1 mM valproic acid (VPA), a histone d...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2019_2814
更新日期:2019-07-09 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::The biodegradability and biocompatibility properties of ε-caprolactone homopolymers place it as a valuable raw material, particularly for controlled drug delivery and tissue engineering applications. However, the usefulness of such materials is limited by their low hydrophilicity and slow biodegradation rate. In order...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract::Melanins form a diverse group of pigments synthesized in living organisms in the course of hydroxylation and polymerization of organic compounds. Melanin production is observed in all large taxa from both Pro- and Eukaryota. The basic functions of melanins are still a matter of controversy and speculation, even though...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:2006-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1976-01-01 00:00:00
abstract::The role of Survivin in the pathogenesis of leukemia was explored in order to discover the effective avenues for gene therapy. Most primary leukemia cells isolated from patients as well as three leukemia cell lines (HL-60, K562, and U937) all expressed Survivin gene. To investigate the relationship between Survivin an...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
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更新日期:2013-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:2007-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:1998-01-01 00:00:00
abstract::Glutathione-S-transferase (GST) a Phase-II drug detoxification enzyme, was detected in Setaria cervi, a bovine filarial parasite. In vitro effect of diethylcarbamazine, butylated hydroxyanisole and phenobarbitone on the GST of adult female S. cervi was assayed by the addition of these compounds in the maintenance medi...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::A conservative and apparently harmless A176V mutation in intracellular S. cerevisiae L-asparaginase (ScerAI) completely abolishes the enzyme activity. Sequence and structural comparisons with type II bacterial L-asparaginases show that the mutated residue is in a very conservative region and plays a vital role in the ...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:1997-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2015_1013
更新日期:2016-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:024904829
更新日期:2002-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:2009-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2020_5500
更新日期:2020-11-26 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2017_2308
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:2009-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
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journal_title:Acta biochimica Polonica
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doi:
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2012-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2016_1265
更新日期:2017-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2014_926
更新日期:2015-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1976-01-01 00:00:00
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journal_title:Acta biochimica Polonica
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doi:045104883
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
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