Abstract：
:The amniotic fluid (AF) was fractionated by dialysis, gel filtration and SDS/PAGE, and submitted to the assay of collagenous constituents. The collagenous character of peptides and proteins of amniotic fluid was confirmed by hydroxyproline (Hyp) assay and treatment with bacterial collagenase followed by electrophoresis and gel filtration of the digestion products. It was found that AF contains collagen degradation products but the classical method of Hyp determination described by Woessner (Arch. Biochem. Biophys., 1961, 93, 440-447) gives overestimated values due to the interference with other AF components. Fractionation of AF on Sephadex G-100 column allowed to remove the interfering material and to estimate the actual Hyp content which equals to approx. 6.2 microg/ml. About 70% of Hyp was found in low molecular dialyzable products and the rest (about 30%) appears to be a constituent of nondialyzable collagenous polypeptides of the molecular mass of about 7.9-26.3 kDa. It is suggested that such collagenous polypeptides may be the products of proteolytic conversion of collagen precursor (procollagen) into the monomeric form of this protein. No high molecular forms of collagen, corresponding to alpha-subunits, were found.
journal_name
Acta Biochim Poljournal_title
Acta biochimica Polonicaauthors
Gogiel T,Bielecki DA,Bańkowski Esubject
Has Abstractpub_date
1998-01-01 00:00:00pages
1037-46issue
4eissn
0001-527Xissn
1734-154Xjournal_volume
45pub_type
杂志文章abstract：:Natural and synthetic derivatives of benzo-γ-pyrones (i.e. flavones, chromones, and coumarins) and their synthetic analogues possess a wide range of biological properties in vitro and in vivo. In this paper we investigated the influence of two hydrazone compounds of chromones, 3-{[(2-dimethoxytiophosphoryl)-2-methylhy...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：:Recently, embryo muscle development, which is crucial for postnatal skeletal muscle growth, has been investigated widely. Nutrients in ovo were suggested to be critical in embryo muscle development since the chick growth mostly relies on nutrients in eggs at the early developmental stage. Phytosterol esters (PE), whic...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：10.18388/abp.2020_2884
更新日期：2020-03-19 00:00:00
abstract：:Microbiological purity control of food products is of great importance in the food industry. Contaminated food is often characterized by a deteriorated taste, smell, and appearance, and when consumed, it can pose a threat to human health and life. Also, contamination incurs huge financial losses to the food industry. ...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：10.18388/abp.2020_5380
更新日期：2020-09-14 00:00:00
abstract：:We here report the influence of the cell cycle abrogator UCN-01 on RKO human colon carcinoma cells differing in p53 status following exposure to two DNA damaging agents, the topoisomerase inhibitors etoposide and camptothecin. Cells were treated with the two drugs at the IC90 concentration for 24 h followed by post-in...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:The influence of exogenous inorganic pyrophosphate on the transport of oleanolic acid monoglucoside and monoglucuronide to vacuoles isolated from Calendula officinalis leaf protoplasts was studied. The results indicate that the transport of both monoglycosides is carrier-mediated; however, the transport of the monoglu...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:All organisms are exposed to numerous stress factors, which include harmful xenobiotics. The diversity of these compounds is enormous, thus in the course of evolution diverse biological defense mechanisms at various levels of organization have developed. One of them engages an evolutionarily conserved family of transp...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：10.18388/abp.2017_2299
更新日期：2017-01-01 00:00:00
abstract：:Inhibition of jack bean activity by 2,5-dichloro-1,4-benzoquinone (DCBQ) was studied in phosphate buffer, pH 7.0. It was found that DCBQ acted as a strong, time and concentration dependent inactivator of urease. Under the experimental conditions obeyed the terms of pseudo-first-order reaction, urease was totally inact...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2011-01-01 00:00:00
abstract：:Trans-diamminedichloroplatinum(II) (trans-DDP) forms with DNA at 37 degrees C, more numerous interstrand cross-links than cis-DDP in the isolated DNA and DNA in the chromatin complex. An increase in the temperature to 42.5 degrees C had no effect on the interstrand cross-links of DND-Pt-DNA formed by the two isomers, ...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:A correlation is known to exist in yeast and other organisms between the cellular resistance to stress and the life span. The aim of this study was to examine whether stress treatment does affect the generative life span of yeast cells. Both heat shock (38 degrees C, 30 min) and osmotic stress (0.3 M NaCl, 1 h) applie...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:Since yeast Saccharomyces cerevisiae mutants depleted of the voltage dependent anion selective channel (YVDAC1) are still able to grow on a non-fermentable carbon source, a functional transport system in the outer mitochondrial membrane must exist to support the access of metabolites into mitochondria. It was assumed ...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:Influenza virus infection is a major source of morbidity and mortality worldwide. Due to the variable effectiveness of existing vaccines, especially in the early stages of an epidemic, antiviral drugs represent the first line of defense against the virus. Currently, there are two major classes of anti-influenza drugs ...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：
更新日期：2014-01-01 00:00:00
abstract：:Cytosine nucleoside deaminase (EC 3.5.4.5) from Salmonella typhimurium LT2 catalyses the deamination of ribo-, deoxyribo- and arabinosyl nucleosides of cytosine alkylated at the C-5, but not at the N3 or exocyclic N4, of the pyrimidine ring. The enzyme was inert towards analogues etherified at the 3'-OH and 5'-OH of t...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1975-01-01 00:00:00
abstract：:Fibrin split product D-dimer (DD) is most probably involved in the development of vascular disorders. At 1.5 microM concentration DD inhibited the incorporation of D-[1-(3)H]glucosamine hydrochloride and [2-(14)C]acetate x Na into pericellular heparan sulphate (HS) of rabbit coronary endothelial cells without affectin...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：035001279
更新日期：2003-01-01 00:00:00
abstract：:This is a short survey of the adaptive mutation processes that arise in non- or slowly-dividing bacterial cells and includes: (i) bacterial models in which adaptive mutations are studied; (ii) the mutagenic lesions from which these mutations derive; (iii) the influence of DNA repair processes on the spectrum of adapti...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：
更新日期：2000-01-01 00:00:00
abstract：:Ammonium salt of N-(dithiocarboxy)sarcosine (DTCS) chelated to ferrous salt was tested as an NO-metric spin trap at room temperature for ex vivo measurement of (.)NO production in murine endotoxaemia. In a chemically defined in vitro model system EPR triplet signals of NO-Fe(DTCS)(2) were observed for as long as 3 hou...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：035003799
更新日期：2003-01-01 00:00:00
abstract：:Yeasts are a convenient platform for many applications. They have been widely used as the expression hosts. There is a need to have a new yeast expression system to contribute the molecular cloning demands. Eight yeast isolates were screened from various environment sources and identified through ribosomal DNA (rDNA) ...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：:The walls of human abdominal aortas and atherosclerosis-induced aneurysms contain similar amounts of collagen. The quantitative ratio between collagens of various types of this protein does not differ significantly either, whereas solubility of the collagen in aneurysmal wall and its susceptibility to the action of ED...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:In contrast to most other eukaryotic organisms, yeast can survive without respiration. This ability has been exploited to investigate nuclear genes required for expression of mitochondrial DNA. Availability of complete Saccharomyces cerevisiae genomic sequence has provided additional help in detailed molecular analysi...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：
更新日期：2000-01-01 00:00:00
abstract：:In the haemolymph of Oniscidea haemocyanin exists mainly in the form of hexamer 16S (90%); the 5S and 24S components are present in small amounts. Alkaline dissociation of the examined 16S haemocyanins is dependent on pH and divalent cations (Ca2+,Mg2+). The 16S component consists of 23-24.5% acidic amino acids, 15-18...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:Several ribonucleoside analogues with modifications in the nucleobase and sugar moiety have been screened for anti-glioma activity in the T98G glioma cell line using cervical (HeLa) cell line as reference human malignant cells, and lung fibroblast (MCR-5) cell line as non-cancerous reference cells. Among the investiga...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：10.18388/abp.2016_1338
更新日期：2016-01-01 00:00:00
abstract：:Spectroscopic, photochemical and biological properties of indocyanine green (ICG) are presented. Light over 800 nm is effectively absorbed by ICG. This property as well as photochemical behaviour of ICG make it a very suitable dye for photodynamic treatment of melanoma cells. Cytotoxicity of ICG itself and the effect ...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Chemical deglycosylation (dg) of sturgeon Acipenser gueldenstaedti Br. (alphaGTH) resulted in the loss of 83% of its initial carbohydrate content. It altered also recombinant dg alphaGTH + betaGTH dimer molecule, reducing its immunoreactivity by 30%, and fully blocking the hormonal function. CD spectroscopy showed tha...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:This study aimed to evaluate the antidotal potency of tenocyclidine (TCP) that probably might protect acetylcholinesterase (AChE) in the case of organophosphate poisoning. TCP was tested alone as a pretreatment or in combination with atropine as a therapy in rats poisoned with (1/4) and (1/2) of LD(50) of soman. Possi...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:1. The everted-sac technique has been used to study the time-dependent effect of low-calcium diet on calcium active transport along rat small intestine. 2. In animals maintained on standard diet active translocation of calcium was limited to proximal 10 cm of the intestine. 3. In response to calcium restriction, calci...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1976-01-01 00:00:00
abstract：:Lanthanides, also called rare-earth elements, are an interesting group of 15 chemically active, mainly trivalent, f-electronic, silvery-white metals. In fact, lanthanides are not as rare as the name implies, except for promethium, a radioactive artificial element not found in nature. The mean concentrations of lanthan...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：
更新日期：2000-01-01 00:00:00
abstract：:Recently Boratyński & Roy (Glycoconjugate J., 1998, 15, 131) described a fast and convenient procedure for the synthesis of glycoconjugates. In the present study we used ESI-MS and circular dichroism as tools to analyze non-enzymatic glycation products of proteins and peptides. We discuss influence of reaction conditi...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Dendrimers are a new class of polymeric materials. They are highly branched, monodisperse macromolecules. The structure of these materials has a great impact on their physical and chemical properties. As a result of their unique behaviour dendrimers are suitable for a wide range of biomedical and industrial applicatio...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：
更新日期：2001-01-01 00:00:00
abstract：:A procedure for purification of oestrone sulphate sulphohydrolase from human placenta microsomes was elaborated. The use of Concanavalin-A-Sepharose chromatography made it possible to separate, for the first time, oestrone sulphate sulphohydrolase (Mr 36,000, optimum pH 7.0, Km 5.5 X 10(-5) M, specific activity 1563 n...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:Signalling concerns the transfer of information from one body, a source, to another, a receiver in order to stimulate activity. The problem arises with the word information. It is defined as what is transferred in a sequence of things, say between people, e.g. words or signs. The idea of signalling between people is t...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：035001281
更新日期：2004-01-01 00:00:00
abstract：:Replication of lambda plasmid DNA is halted in amino acid-starved wild type (stringent) strains whereas it proceeds in relA (relaxed) mutants. The only transcription which could be important in lambda plasmid DNA replication in amino acid-starved Escherichia coli cells is that starting from the pR promoter. Using a fu...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
