Abstract：
:The extent to which early differentiation of neurons and muscle cells is autonomous or governed by soluble factors released from other cells has been examined by following development of single cells plated alone in a simple, defined culture medium. The differentiation of electrical excitability and sensitivity to neurotransmitters of amphibian spinal neurons and trunk muscle in Xenopus embryos has already been described. For both cell types, differentiation in cultures containing relatively large numbers of dispersed cells parallels development in vivo, with respect to qualitative changes in membrane properties and the time course of development. Cell contacts are not required for this process. Here we show that the differentiation of membrane properties of single, isolated cells exhibits a similar set of changes, although muscle cells develop more slowly in some respects and all cells survive for a shorter period of time. The results suggest that the continued presence of specific extracellular differentiation-promoting factors is not required for these early steps of neuronal development, although a role for such factors in development of myocytes cannot be excluded. In contrast, survival factors secreted by other cells may be necessary to prolong the lifetimes of dissociated cells.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Henderson LP,Spitzer NCdoi
10.1016/0012-1606(86)90173-9subject
Has Abstractpub_date
1986-02-01 00:00:00pages
381-7issue
2eissn
0012-1606issn
1095-564Xpii
0012-1606(86)90173-9journal_volume
113pub_type
杂志文章abstract：:The MLH1 protein is required for normal meiosis in mice and its absence leads to failure in maintenance of pairing between bivalent chromosomes, abnormal meiotic division, and ensuing sterility in both sexes. In this study, we investigated whether failure to develop foci of MLH1 protein on chromosomes in prophase woul...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0708
更新日期：2002-09-01 00:00:00
abstract：:We have examined the presence of protein kinase C in oocytes of Chaetopterus pergamentaceus and its role in the initiation of germinal vesicle breakdown (GVBD). First, we demonstrated that the oocytes contain a phospholipid- and calcium-dependent protein kinase, protein kinase C (PKC). Since PKC is the primary intrace...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90294-q
更新日期：1992-02-01 00:00:00
abstract：:Brain 4 (Brn4/Pou3f4) and Pax6 are POU-homeodomain and paired-homeodomain transcription factors, respectively, that are expressed in the brain and the glucagon-expressing cells in the pancreas. Brn4 expression begins at embryonic day 10 in the pancreas, just before pax6 and both appear in the glucagon immunoreactive c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.12.008
更新日期：2004-04-01 00:00:00
abstract：:Sensory placodes are unique columnar epithelia with neurogenic potential that develop in the vertebrate head ectoderm next to the neural tube. They contribute to the paired sensory organs and the cranial sensory ganglia generating a wide variety of cell types ranging from lens fibres to sensory receptor cells and neur...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2004.08.037
更新日期：2004-12-01 00:00:00
abstract：:A new monoclonal antibody, specific to an epitope in the carboxyl terminus of the Drosophila collagen IV molecule (basement membrane collagen) was identified. The distributions of collagen IV, laminin, and an additional extracellular molecule, the 2G2 antigen (2G2-Ag), were followed immunocytochemically during early w...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1068
更新日期：1995-03-01 00:00:00
abstract：:Choroideremia (CHM) is a hereditary eye disease caused by mutations in the X-linked CHM gene. Disruption of the Chm gene in mice resulted in prenatal death of Chm-/Y males and Chm-/Chm+ females that had inherited the mutation from their mothers. Male chimeras and Chm+/Chm- females with paternal transmission of the mut...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.04.016
更新日期：2004-08-01 00:00:00
abstract：:In warm-blooded vertebrates it is generally accepted that after early stages of development new neurons are not added to the retina. Contrary to this belief, we show here that hatched chickens have a zone of proliferating cells at the peripheral margin of the retina, similar to that of fish and amphibians. We found th...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9640
更新日期：2000-04-15 00:00:00
abstract：:Pericytes heterogeneity is based on their morphology, distribution, and markers. It is well known that pericytes from different organs may have distinct embryonic sources. Yamazaki et al. (2017) using several transgenic mouse model reveal by cell-lineage tracing that pericytes are even more heterogeneous than previous...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.05.001
更新日期：2017-07-01 00:00:00
abstract：:Cytoskeletal intermediate filaments were studied in muscular dysgenesis (mdg) and tetrodotoxin-treated inactive mouse embryo muscle cultures during myofibrillogenesis. Both muscular dysgenesis and tetrodotoxin-treated muscles are characterized in vitro by a total lack of contractile activity and an abnormal developmen...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90159-5
更新日期：1988-09-01 00:00:00
abstract：:During metamorphosis, the steroid hormone 20-hydroxyecdysone induces morphogenesis of imaginal discs, including the formation of appendages. IMP-E2 is an ecdysone-dependent, single-copy Drosophila gene, whose transcripts accumulate rapidly in imaginal discs in response to the hormone. The IMP-E2 product is secreted at...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90084-v
更新日期：1990-08-01 00:00:00
abstract：:Gene network analysis requires computationally based models which represent the functional architecture of regulatory interactions, and which provide directly testable predictions. The type of model that is useful is constrained by the particular features of developmentally active cis-regulatory systems. These systems...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1006/dbio.2002.0617
更新日期：2002-06-01 00:00:00
abstract：:The corepressor SMRT acts on a range of transcription factors, including the retinoid and thyroid hormone nuclear receptors. The carboxy-terminal region of SMRT contains CoRNR box motifs that mediate these interactions. We have shown, in Xenopus, that SMRT can exist as isoforms containing either two or three CoRNR box...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.01.007
更新日期：2006-04-15 00:00:00
abstract：:In developing limb buds, Msx2 transcripts are expressed in the apical ectodermal ridge (AER) and in various regions of the limb mesenchyme. To identify DNA sequences responsible for Msx2 expression in the AER, we characterized the expression of LacZ reporter constructs driven by chicken Msx2 regulatory sequences in tr...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.03.005
更新日期：2004-06-15 00:00:00
abstract：:The segregation of founder cells from the somatic mesoderm is a prerequisite for the formation of body wall muscles in the Drosophila embryo. The myogenic basic helix-loop-helix protein, Nautilus (Nau), is expressed in a subset of these founder cells in medial and lateral positions in the somatic mesoderm. Mutations i...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.9987
更新日期：1996-05-25 00:00:00
abstract：:Experiments in which the developing gut of avian embryos was back-transplanted to permit the bowel to interact with the developing neural tube were undertaken. Segments of intestine from 4-day quail embryos were implanted between the somites and neural tubes of chick embryos of 7 to 24 somites. The spinal cord respond...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90486-6
更新日期：1987-12-01 00:00:00
abstract：:The mouse prostate gland develops by branching morphogenesis from the urogenital epithelium and mesenchyme. Androgens and developmental factors, including FGF10 and SHH, promote prostate growth (Berman, D.M., Desai, N., Wang, X., Karhadkar, S.S., Reynon, M., Abate-Shen, C., Beachy, P.A., Shen, M.M., 2004. Roles for He...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.08.018
更新日期：2005-12-15 00:00:00
abstract：:The protein tyrosine phosphatase PTP1, which mediates reversible phosphorylation on tyrosine, has been shown to play an important regulatory role during Dictyostelium development. Mutants lacking PTP1 develop more rapidly than normal, while strains that overexpress PTP1 display aberrant morphology. However, the signal...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0171
更新日期：2001-04-01 00:00:00
abstract：:Expression of constitutively active Src protein tyrosine kinase in Xenopus oocytes has been shown to accelerate oocyte maturation suggesting that Src may be involved in meiotic progression. However, meiotic regulation of endogenous Src kinase in oocytes has not been investigated in detail. To address this problem, we ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.10.018
更新日期：2005-02-15 00:00:00
abstract：:The Drosophila brain is generated by a complex series of morphogenetic movements. To better understand brain development and to provide a guide for experimental manipulation of brain progenitors, we created a fate map using photoactivated gene expression to mark cells originating within specific mitotic domains and ti...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00220-3
更新日期：2003-08-01 00:00:00
abstract：:Gnathostomes (jawed vertebrates) possess skeletal muscles with unique functional and developmental features that are absent from cyclostomes-i.e., lamprey and hagfish. These gnathostome-specific traits include the epaxial and hypaxial division of myotomes, paired fin/limb muscles, shoulder girdle muscles, and the musc...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.10.029
更新日期：2011-02-01 00:00:00
abstract：:Vertebrate pigment cells are derived from neural crest, a tissue that also forms most of the peripheral nervous system and a variety of ectomesenchymal cell types. Formation of pigment cells from multipotential neural crest cells involves a number of common developmental processes. Pigment cells must be specified; the...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9840
更新日期：2000-09-15 00:00:00
abstract：:Hippo (Hpo) signaling plays a critical role in restricting tissue growth and organ size in both invertebrate and vertebrate animals. However, how the Hpo kinase is regulated during development has not been clearly understood. Using a Bimolecular Fluorescence Complementation assay, we have investigated the functional s...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.12.017
更新日期：2013-03-15 00:00:00
abstract：:Intestinal tract development is a coordinated process involving signaling among the progenitors and developing cells from all three germ layers. Development of endoderm-derived intestinal epithelium has been shown to depend on epigenetic modifications, but whether that is also the case for intestinal tract cell types ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.08.002
更新日期：2019-11-15 00:00:00
abstract：:This first study of the onset of spermatogenesis in the sea urchin, Strongylocentrotus purpuratus, was undertaken using individuals reared in the laboratory. Spermatogenesis commences about 11-12 months after metamorphosis in these animals. Bindin message accumulates in late spermatocytes and early spermatids which li...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90149-d
更新日期：1990-11-01 00:00:00
abstract：:Activation of progenitor cells is crucial to promote tissue repair following injury in adult animals. In the context of successful limb regeneration following amputation, progenitor cells residing within the stump must re-enter the cell cycle to promote regrowth of the missing limb. We demonstrate that in axolotls, am...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.07.010
更新日期：2018-01-15 00:00:00
abstract：:Changes in the intracellular free calcium levels in the cortical region of cleaving Xenopus eggs were examined using semi-synthetic aequorin. We detected periodic oscillations in the free calcium levels accompanying the cleavage cycle as early as the first cleavage onward. The calcium levels began to increase at early...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1325
更新日期：1993-12-01 00:00:00
abstract：:The nematode Caenorhabditis elegans has retained a rudimentary Hedgehog (Hh) signalling pathway; Hh and Smoothened (Smo) homologs are absent, but two highly related Patched gene homologs, ptc-1 and ptc-3, and 24 ptc-related (ptr) genes are present. We previously showed that ptc-1 is essential for germ line cytokinesis...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.12.035
更新日期：2011-03-15 00:00:00
abstract：:We have used in situ hybridization and RNA blotting analysis to compare the timing of accumulation of poly(A) and alpha-subtype histone mRNA during oogenesis in the sea urchin Strongylocentrotus purpuratus. In situ hybridization with 3H-poly(U) shows that the content of poly(A) in the developing oocyte increases four-...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90161-1
更新日期：1984-02-01 00:00:00
abstract：:Interleukin-6 is a member of a class of hormone-like molecules termed cytokines. The actions of IL-6 are highly pleiotropic. In adults IL-6 functions as a major mediator of inflammatory responses as well as inducing the synthesis of acute phase proteins by the liver following infection or injury. Based on in vitro and...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/0012-1606(92)90173-e
更新日期：1992-06-01 00:00:00
abstract：:The mammalian Cone-rod homeobox (Crx) gene is a divergent member of the Otx gene family known to be involved in differentiation and survival of retinal photoreceptors and photoentrainment of circadian rhythms. Zebrafish have two genes in the Otx5/crx orthology class, and we previously showed that crx can transactivate...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.01.037
更新日期：2004-05-01 00:00:00
