First complete genome sequence of Bacillus glycinifermentans B-27.

abstract：:The growth of bacteria as structured aggregates termed biofilms leads to their protection from harsh environmental conditions such as physical and chemical stresses, shearing forces, and limited nutrient availability. Because of this highly adapted ability to survive adverse environmental conditions, bacterial biofilm...

journal_title：Journal of biotechnology

authors： Taylor PK,Yeung AT,Hancock RE

更新日期：2014-12-10 00:00:00

abstract：:Pure wine yeast cultures are increasingly used in winemaking to perform controlled fermentations and produce wine of reproducible quality. For the genetic manipulation of natural wine yeast strains dominant selective markers are obviously useful. Here we demonstrate the successful use of the mutated PDR3 gene as a dom...

journal_title：Journal of biotechnology

authors： Kozovska Z,Maraz A,Magyar I,Subik J

更新日期：2001-12-14 00:00:00

abstract：:The factors which limit the proliferation of eukaryotic cells in vitro are still not well known. Ammonia is believed to be toxic for mammalian cell proliferation and secretion. We have tried two approaches to reducing the ammonia in the medium. We first limited the ammonia produced by the cells by replacing glutamine ...

journal_title：Journal of biotechnology

authors： Capiaumont J,Legrand C,Carbonell D,Dousset B,Belleville F,Nabet P

更新日期：1995-02-21 00:00:00

abstract：:5-Methyluridine was produced continuously from thymine and inosine by immobilized enzymes, which consisted of thermostable purine nucleoside phosphorylase and thermostable pyrimidine nucleoside phosphorylase obtained from Bacillus stearothermophilus JTS 859. The process was carried out in a column reactor at 60 degree...

journal_title：Journal of biotechnology

authors： Hori N,Watanabe M,Sunagawa K,Uehara K,Mikami Y

更新日期：1991-02-01 00:00:00

abstract：:An NADH-dependent phenylpyruvate reductase (LaPPR) was identified through screening the shotgun library of Lactobacillus sp. CGMCC 9967. It belongs to D-3-phosphoglycerate dehydrogenase (PGDH) subfamily of 2-hydroxy acid dehydrogenase superfamily. LaPPR was stable at pH 6.5 and 30 °C, with a half-life of 152 h. LaPPR ...

journal_title：Journal of biotechnology

authors： Xu GC,Zhang LL,Ni Y

更新日期：2016-03-20 00:00:00

abstract：:A system for streptavidin detection using biotin conjugated to nano-sized bacterial magnetic particles (BMPs) has been developed. BMPs, isolated from magnetic bacteria, were used as magnetic markers for magnetic force microscopy (MFM) imaging. The magnetic signal was obtained from a single particle using MFM without a...

journal_title：Journal of biotechnology

authors： Amemiya Y,Tanaka T,Yoza B,Matsunaga T

更新日期：2005-11-21 00:00:00

abstract：:The successful transfer of human embryonic stem cell (hESC) technology and cellular products into clinical and industrial applications needs to address issues of automation, standardization and the generation of relevant cell numbers of high quality. In this study, we combined microcarrier technology and controlled st...

journal_title：Journal of biotechnology

authors： Serra M,Brito C,Sousa MF,Jensen J,Tostões R,Clemente J,Strehl R,Hyllner J,Carrondo MJ,Alves PM

更新日期：2010-08-02 00:00:00

abstract：:As the important chiral building block of levetiracetam, the synthesis of (S)-2-aminobutanamide has attracted a great deal of attention. The d-aminopeptidase catalyzed kinetic resolution of 2-aminobutanamide was demonstrated as an effective strategy for (S)-2-aminobutanamide production. In this study, a novel d-aminop...

journal_title：Journal of biotechnology

authors： Tang XL,Lu XF,Wu ZM,Zheng RC,Zheng YG

更新日期：2018-01-20 00:00:00

abstract：:Transformed cell cultures of Salvia miltiorrhiza were established by infecting sterile plantlets with Agrobacterium tumefaciens strain C58. The transformed cells in suspension formed macroscopic clumps of cell aggregates up to 2-3 cm in size rather than homogeneous cell suspensions. These transformed cells grew well i...

journal_title：Journal of biotechnology

authors： Chen H,Yuan JP,Chen F,Zhang YL,Song JY

更新日期：1997-12-03 00:00:00

abstract：:Nanobiotechnological improvements defined on the utilization of biological materials and principles have enormously partaken to revolutionize physical, chemical, and biological sciences. However, the exploration of plant nanobiotechnology is still in its outset. The search for novel tools to monitor plant biomolecules...

journal_title：Journal of biotechnology

authors： Ambrin G,Kausar H,Ahmad A

更新日期：2020-11-10 00:00:00

abstract：:This study demonstrates the applicability of pressurized stirred tank bioreactors for oxygen transfer enhancement in aerobic cultivation processes. The specific power input and the reactor pressure was employed as process variable. As model organism Escherichia coli, Arxula adeninivorans, Saccharomyces cerevisiae and ...

journal_title：Journal of biotechnology

authors： Knoll A,Bartsch S,Husemann B,Engel P,Schroer K,Ribeiro B,Stöckmann C,Seletzky J,Büchs J

更新日期：2007-10-31 00:00:00

abstract：:The kinetics of microbial growth and the biodegradation of methanol and toluene in (a) biofilters (BFs), and (b) biotrickling filters (BTFs), packed with inert materials, has been studied and analyzed. The specific growth rate, mu, for the treatment of methanol was 0.037h(-1) for a wide range of operating conditions. ...

journal_title：Journal of biotechnology

authors： Avalos Ramirez A,Bénard S,Giroir-Fendler A,Jones JP,Heitz M

更新日期：2008-11-25 00:00:00

abstract：:Enterokinase, a two-chain duodenal serine protease, activates trypsinogen by removing its N-terminal propeptide. Due to a clean cut after the non-primed site recognition sequence, the enterokinase light chain is frequently employed in biotechnology to separate N-terminal affinity tags from target proteins with authent...

journal_title：Journal of biotechnology

authors： Skala W,Goettig P,Brandstetter H

更新日期：2013-12-01 00:00:00

abstract：:Production of heterologous proteins in bacteria is one of the main applications of biotechnology. Although several high-efficiency expression systems have been developed, different steps in protein production may become rate-limiting depending on the production system and the protein being produced. One bottle neck ca...

journal_title：Journal of biotechnology

authors： Hienonen E,Romantschuk M,Fenel F,Taira S

更新日期：2007-02-01 00:00:00

abstract：:A novel method for immobilizing large DNA fragments on a solid surface was developed. A mixed self-assembled monolayer of thiolated single-stranded DNA with inert alkanethiol was generated on a gold (Au) surface through the Au-S reaction. Surface-tethered DNA generated by this method was compatible with various geneti...

journal_title：Journal of biotechnology

authors： Kim JH,Hong JA,Yoon M,Yoon MY,Jeong HS,Hwang HJ

更新日期：2002-07-03 00:00:00

abstract：:The B subunit of Escherichia coli heat-labile toxin (LTB) is a potent mucosal immunogen and immunoadjuvant for co-administered antigens. In order to produce large scale of LTB for the development of edible vaccine, we used transgenic somatic embryos of Siberian ginseng, which is known as medicinal plant. When transgen...

journal_title：Journal of biotechnology

authors： Kang TJ,Lee WS,Choi EG,Kim JW,Kim BG,Yang MS

更新日期：2006-01-24 00:00:00

abstract：:Terminal sialic acid on oligosaccharides of glycoproteins shows several biological functions of the glycoproteins. The yeast Pichia pastoris normally does not contain sialic acid on the oligosaccharides of glycoproteins. A sialyltransferase (ST) gene was transfected into P. pastoris to assess the possibility of using ...

journal_title：Journal of biotechnology

authors： Chotigeat W,Chayanunnukul W,Phongdara A

更新日期：2000-07-28 00:00:00

abstract：:The quantitative description of the relationships between global properties, defined at the cellular population level, and individual properties, defined at the single cell level, is considered in this communication along with the analysis of some segregated models of yeast and hybridoma cell cultures. ...

journal_title：Journal of biotechnology

authors： Cazzador L

更新日期：1999-05-28 00:00:00

abstract：:A fibrous-bed bioreactor containing the coculture of Pseudomonas putida and P. fluorescens immobilized in a fibrous matrix was developed to degrade benzene (B), toluene (T), ethylbenzene (E), and o-xylene (X) in synthetic waste streams. The kinetics of BTEX biodegradation by immobilized cells adapted in the fibrous-be...

journal_title：Journal of biotechnology

authors： Shim H,Yang ST

更新日期：1999-01-22 00:00:00

abstract：:The first problem in gene expression profiling to be solved is choosing the appropriate gene array, detection procedure, image analysis and data generation depending on the organism of interest, equipment and budget. The next one is how to deduce biologically meaningful data. We assessed gene expression data from chem...

journal_title：Journal of biotechnology

authors： Fahnert B,Hahn D,Guthke R

更新日期：2002-03-14 00:00:00

abstract：:Plasmid DNA purification strategies are often based on chromatographic processes, which often suffer from low dynamic binding capacities and poor productivity. The limitations of conventional chromatography media for adsorption of pDNA led us to the investigation of irregular polymeric microparticles with quaternary a...

journal_title：Journal of biotechnology

authors： Paril C,Horner D,Ganja R,Jungbauer A

更新日期：2009-04-20 00:00:00

abstract：:The present proof-of-concept study reports the construction of a whole-cell biocatalyst for the de novo production of ω-hydroxy octanoic acid. This was achieved by hijacking the natural fatty acid cycle and subsequent hydroxylation using a specific monooxygenase without the need for the additional feed of alkene-like ...

journal_title：Journal of biotechnology

authors： Kirtz M,Klebensberger J,Otte KB,Richter SM,Hauer B

更新日期：2016-07-20 00:00:00

abstract：:A PHA producing microbial culture dominated by Plasticicumulans acidivorans was enriched in a pilot plant using fermented wastewater from the Mars candy bar factory. The pilot plant comprised (1) anaerobic fermentation, (2) enrichment of a PHA-producing microbial community and (3) accumulation for maximization of the ...

journal_title：Journal of biotechnology

authors： Tamisa J,Lužkov K,Jiang Y,van Loosdrecht MC,Kleerebezem R

更新日期：2014-12-20 00:00:00

abstract：:Group I grass pollen allergens are major contributors to grass pollen-related seasonal allergic rhinitis, and as such a primary target for allergen specific immunotherapy. In this study the potential therapeutic role of oral application of Lactobacillus plantarum WCFS1, directing cell wall attachment of the recombinan...

journal_title：Journal of biotechnology

authors： Minic R,Gavrovic-Jankulovic M,Petrusic V,Zivkovic I,Eijsink VG,Dimitrijevic L,Mathiesen G

更新日期：2015-04-10 00:00:00

abstract：:For pre-clinical evaluation of biotherapeutic candidates, protein production by transient gene expression (TGE) in Chinese Hamster Ovary (CHO) cells offers important advantages, including the capability of rapidly and cost-effectively generating recombinant proteins that are highly similar to those produced in stable ...

journal_title：Journal of biotechnology

authors： Stuible M,Burlacu A,Perret S,Brochu D,Paul-Roc B,Baardsnes J,Loignon M,Grazzini E,Durocher Y

更新日期：2018-09-10 00:00:00

abstract：:During tumour development, macrophages are recruited to the tumour site and orientated towards an anti-inflammatory phenotype. Due to their immunosuppressive function, tumour associated macrophages (TAMs) are recognized as major components in tumour progression. Changing these macrophages to a pro-inflammatory phenoty...

journal_title：Journal of biotechnology

authors： Rodet F,Capuz A,Hara T,van Meel R,Duhamel M,Rose M,Raffo-Romero A,Fournier I,Salzet M

更新日期：2018-09-20 00:00:00

abstract：:We report the complete genome sequence of Amycolatopsis japonica MG417-CF17(T) (=DSM 44213(T)) which was identified as the producer of (S,S)-N,N'-ethylenediaminedisuccinic acid during a screening for phospholipase C inhibitors. The genome of A. japonica MG417-CF17(T) consists of two replicons: the chromosome (8,961,31...

journal_title：Journal of biotechnology

authors： Stegmann E,Albersmeier A,Spohn M,Gert H,Weber T,Wohlleben W,Kalinowski J,Rückert C

更新日期：2014-11-10 00:00:00

abstract：:In spite of high prices for fossil raw materials the production of biomass-based products is rarely economically successful today. Depending on the location feedstock prices are currently so high that products from renewable resources are not marketable when produced in existing process chains. Apart from the higher f...

journal_title：Journal of biotechnology

authors： Lyko H,Deerberg G,Weidner E

更新日期：2009-06-01 00:00:00

abstract：:We report the design and characterization of novel fusion proteins, consisting of the F8 antibody and of murine calreticulin (Calr). The F8 antibody recognizes the alternatively-spliced ED-A domain of fibronectin, an extracellular matrix component found in most tumor types, while calreticulin has previously been descr...

journal_title：Journal of biotechnology

authors： Ziffels B,Grötsch A,Al-Bayati L,Neri D

更新日期：2019-01-20 00:00:00

abstract：:The purification of human antibodies in an aqueous two-phase system (ATPS) composed of polyethylene glycol (PEG) 6000 and phosphate was optimised by surface response methodology. A central composite design was used to evaluate the influence of phosphate, PEG and NaCl concentration and of the pH on the purity and extra...

journal_title：Journal of biotechnology

authors： Azevedo AM,Rosa PA,Ferreira IF,Aires-Barros MR

更新日期：2007-10-31 00:00:00