Enrichment of Plasticicumulans acidivorans at pilot-scale for PHA production on industrial wastewater.

Abstract:

:A PHA producing microbial culture dominated by Plasticicumulans acidivorans was enriched in a pilot plant using fermented wastewater from the Mars candy bar factory. The pilot plant comprised (1) anaerobic fermentation, (2) enrichment of a PHA-producing microbial community and (3) accumulation for maximization of the cellular PHA content. After anaerobic fermentation, the wastewater contained mainly VFAs (0.64 ± 0.15 gCOD/gCOD) and ethanol (0.22 ± 0.13 gCOD/gCOD). In the enrichment reactor (cycle 12 h, SRT 24 h) a feast-famine pattern was established with a feast phase of around 35 ± 5 min. The culture was able to accumulate 0.70 ± 0.05 gPHA/gVSS. The difference with previous lab-scale results from P. acidivorans, in which a PHA content of 0.90 gPHA/gVSS was achieved, could be attributed to the presence of solids in the influent, the growth of a side population and the accumulation of non-PHA storage compounds that appeared to be related to ethanol consumption.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Tamisa J,Lužkov K,Jiang Y,van Loosdrecht MC,Kleerebezem R

doi

10.1016/j.jbiotec.2014.10.022

subject

Has Abstract

pub_date

2014-12-20 00:00:00

pages

161-9

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(14)00914-6

journal_volume

192 Pt A

pub_type

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