Iron-molybdenum cofactor of nitrogenase: electrochemical determination of the electron stoichiometry of the oxidized/semi-reduced couple.

abstract：:Leda-1/Pianp is a type I transmembrane protein expressed by CNS cells, murine melanoma cell line B16F10 and rat liver sinusoidal endothelial cells. The early steps of posttranslational modifications of Leda-1/Pianp have been described to include glycosylation and processing by proprotein convertases. Here, we comprehe...

journal_title：Biochemical and biophysical research communications

authors： Biswas S,Adrian M,Weber J,Evdokimov K,Winkler M,Géraud C

更新日期：2016-09-02 00:00:00

abstract：:Alkaline elastase YaB, produced by alkalophilic Bacillus YaB, is an extracellular serine protease having 55% homology to subtilisin BPN' and thus could be called subtilisin YaB. It is synthesized as a 378-amino acid preproenzyme and secreted into the culture medium as a 265-amino acid mature protease. To examine if th...

journal_title：Biochemical and biophysical research communications

authors： Chang YC,Kadokura H,Yoda K,Yamasaki M

更新日期：1996-02-15 00:00:00

abstract：:Herpes simplex virus-1 (HSV-1) glycoprotein E (gE) is a multifunctional protein capable of both binding the Fc portion of IgG and mediating cell-to-cell spread of HSV-1. Here we report that the domain on gE involved in IgG binding is distinct from the domain involved in mediating cell-to-cell spread. To do this we hav...

journal_title：Biochemical and biophysical research communications

authors： Weeks BS,Sundaresan P,Nagashunmugam T,Kang E,Friedman HM

更新日期：1997-06-09 00:00:00

abstract：:Primary mouse embryo fibroblasts passed in culture go through a period of declining growth rate and then after a period of minimal proliferation begin to grow again, generating, under appropriate conditions, an immortal 3T3 line. The 3T3 cells, but not the primary mouse embryo fibroblasts, are able to synthesize and t...

journal_title：Biochemical and biophysical research communications

authors： Edwards DR,Parfett CL,Smith JH,Denhardt DT

更新日期：1987-08-31 00:00:00

abstract：:Earlier investigations have shown that (a) antibodies against a carrier-coupled 20-residue synthetic peptide (C-20), (200)HACQKKLLKFEALQQEEGEE(219), corresponding to the C-terminal partially helical sequence of chicken riboflavin carrier protein (RCP; 219 AA) curtail pregnancy in mammals and (b) helix stabilization by...

journal_title：Biochemical and biophysical research communications

authors： Subramanian S,Karande AA,Adiga PR

更新日期：2001-09-14 00:00:00

abstract：:We investigated the binding and pharmacologic properties of peptides encoded by complementary mRNA derived from the human and rat angiotensinogen gene (human and rat IIA, respectively). Human IIA (identical with AII in 4 amino acids) inhibited binding of [125I]AII to rat adrenal glomerulosa particles (Ki = 0.62 +/- 0....

journal_title：Biochemical and biophysical research communications

authors： Wiest SA,Gesellchen PD,Zimmerman K,Steinberg MI

更新日期：1990-07-31 00:00:00

abstract：:WNK kinases are novel serine/threonine protein kinases. Mutations in two members of the WNK family, WNK1 and WNK4, cause familial hyperkalemic hypertension. These kinases regulate ion transport across diverse epithelia; WNK4 reduces activity of the Na-Cl cotransporter activity and the potassium channel, ROMK, by reduc...

journal_title：Biochemical and biophysical research communications

authors： Wang Z,Yang CL,Ellison DH

更新日期：2004-05-07 00:00:00

abstract：:The molecular structures of enzyme mimics may be modified to optimize their catalytic properties. In this study, to generate artificial enzyme mimics, Watson-Crick base paired DNA duplexes were designed as scaffolds which were assembled by nucleotides modified with specific functional groups. This process allowed vari...

journal_title：Biochemical and biophysical research communications

authors： Xu L,Ji C,Bai Y,He J,Liu K

更新日期：2013-05-10 00:00:00

abstract：:Megakaryocytes isolated in high purity from guinea pigs produced thromboxane B2 in response to exogenously provided arachidonic acid. This production was inhibited by in vitro treatment with acetylsalicylic acid with a concentration response relationship similar to that seen in platelets. During in vitro culture, the ...

journal_title：Biochemical and biophysical research communications

authors： Walenga RW,Miller JL,Stuart MJ

更新日期：1984-03-30 00:00:00

abstract：:Carboxysome, encapsulating an enzymatic core within an icosahedral-shaped semipermeable protein shell, could enhance CO2 fixation under low CO2 conditions in the environment. The shell of Halothiobacillus neapolitanus α-carboxysome possesses two 38% sequence-identical pentameric proteins, namely CsoS4A and CsoS4B. How...

journal_title：Biochemical and biophysical research communications

authors： Zhao YY,Jiang YL,Chen Y,Zhou CZ,Li Q

更新日期：2019-07-30 00:00:00

abstract：:Four monoclonal antibodies, M-T21, M-T32, M-T41 and UM4D4, which belong to the new CDw 60 cluster of antibodies specific for a subpopulation of human T-lymphocytes, were found to bind mainly to acetylated forms of ganglioside GD3. After O-deacetylation of the antigen, binding was reduced ("M-T"-antibodies) or abolishe...

journal_title：Biochemical and biophysical research communications

authors： Kniep B,Peter-Katalinić J,Flegel W,Northoff H,Rieber EP

更新日期：1992-09-30 00:00:00

abstract：:Liposome-encapsulated clodronate (LEC) is a specific depletor of macrophages. Our study characterized the LEC depletory effects, given intrathecally, on spinal microglia and assessed its effects on initiation and maintenance of neuropathic pain. Measured by using the MTT assay, LEC treatment specifically inhibited cel...

journal_title：Biochemical and biophysical research communications

authors： Wang YR,Mao XF,Wu HY,Wang YX

更新日期：2018-05-15 00:00:00

abstract：:Treatment with double-stranded RNA (dsRNA) has been shown to interfere with the function of specific genes in various invertebrate species. However, it has not yet been reported that this technique can be applied to vertebrates as well. We have investigated whether dsRNA treatment will inhibit gene function in zebrafi...

journal_title：Biochemical and biophysical research communications

authors： Wargelius A,Ellingsen S,Fjose A

更新日期：1999-09-16 00:00:00

abstract：:A low molecular weight protein (approximately 25,000 D) exhibiting a yellow fluorescence emission peaking at approximately 540 nm was isolated from Vibrio fischeri (strain Y-1) and purified to apparent homogeneity. FMN is the chromophore, but it exhibits marked red shifts in both the absorption (lambda max = 380, 460 ...

journal_title：Biochemical and biophysical research communications

authors： Macheroux P,Schmidt KU,Steinerstauch P,Ghisla S,Colepicolo P,Buntic R,Hastings JW

更新日期：1987-07-15 00:00:00

abstract：:1,25-Dihydroxyvitamin D(3) [1,25(OH)(2)D(3)] initiates the hydrolysis of sphingomyelin in ROS 17/2.8 osteosarcoma cells with the resultant generation of cell-associated ceramide. Increases in ceramide levels were detectable at 15 min and maximal one hour after exposure of cells to 1,25(OH)(2)D(3). Neither 1,25(OH)(2)D...

journal_title：Biochemical and biophysical research communications

authors： Liu R,Xu Y,Farach-Carson MC,Vogel JJ,Karin NJ

更新日期：2000-06-24 00:00:00

abstract：:The bacteriophage T7 binary expression system is widely used in vitro for high level selective expression of cloned genes but its application to in vivo models has not yet been investigated. In the present work, we show that coinjection into fertilized zebrafish eggs of pE1T7R, an expression plasmid bearing the T7 RNA...

journal_title：Biochemical and biophysical research communications

authors： Verri T,Argenton F,Tomanin R,Scarpa M,Storelli C,Costa R,Colombo L,Bortolussi M

更新日期：1997-08-28 00:00:00

abstract：:PSI domains are cysteine-rich modules found in extracellular fragments of hundreds of signaling proteins, including plexins, semaphorins, integrins, and attractins. Here, we report the solution structure of the PSI domain from the human Met receptor, a receptor tyrosine kinase critical for proliferation, motility, and...

journal_title：Biochemical and biophysical research communications

authors： Kozlov G,Perreault A,Schrag JD,Park M,Cygler M,Gehring K,Ekiel I

更新日期：2004-08-13 00:00:00

abstract：:Actin bundling protein 34 (ABP34) is the one of 11 actin-crosslinking proteins identified in Dictyostelium discoideum, a novel model organism for the study of actin-associated neurodegenerative disorders such as Alzheimer's disease and Huntington's disease. ABP34 localizes at the leading and trailing edges of locomoto...

journal_title：Biochemical and biophysical research communications

authors： Chung JM,Kim HU,Kim GJ,Jeoung D,Jung HS

更新日期：2018-09-10 00:00:00

abstract：:Molecular cloning of targeting vectors (TgVs) is a prerequisite procedure for gene disruption in embryonic stem cells. In cases where target genes display complex features (e.g., gene overlap, alternative exon usage), TgVs must mediate deletions with very high precision to prevent unwanted effects. This is often diffi...

journal_title：Biochemical and biophysical research communications

authors： Miazek A,Cebula A,Skwarek M,Cebrat M,Kisielow P

更新日期：2007-06-29 00:00:00

abstract：:Caspase-8 is a key mediator in various biological processes such as apoptosis, necroptosis, inflammation, T/B cells activation, and cell motility. Caspase-8 is characterized by the N-terminal tandem death effector domains (DEDs) and the C-terminal catalytic protease domain. The DEDs mediate diverse functions of caspas...

journal_title：Biochemical and biophysical research communications

authors： Shen C,Yue H,Pei J,Guo X,Wang T,Quan JM

更新日期：2015-07-31 00:00:00

abstract：:Human bone marrow cells express both a truncated and full-length form of the erythropoietin receptor (EpoR-T and EpoR-F, respectively). Transfection experiments using the murine interleukin (IL)-3-dependent cell line, Ba/F3, revealed that the cells coexpressing EpoR-F and EpoR-T (Ba/F3-FT) were more likely to undergo ...

journal_title：Biochemical and biophysical research communications

authors： Shimizu R,Komatsu N,Nakamura Y,Nakauchi H,Nakabeppu Y,Miura Y

更新日期：1996-05-06 00:00:00

abstract：:In this work, we examined the functions of the female hormone "estrogen" on spermatogenesis of the Japanese eel (Anguilla japonica). Estradiol-17beta (E(2)), a natural estrogen in vertebrates, was present in the serum and its receptor was expressed in the testis during the whole process of spermatogenesis. Spermatogon...

journal_title：Biochemical and biophysical research communications

authors： Miura T,Miura C,Ohta T,Nader MR,Todo T,Yamauchi K

更新日期：1999-10-14 00:00:00

abstract：:More than 85% of c-myc transcription is controlled by the nuclease hypersensitive element III(1) upstream of the P1 promoter of this oncogene. The purine-rich sequence in the anti-sense strand forms a G-quadruplex, which has been recently implicated in colorectal cancer, and is proposed as a silencer element [Proc. Na...

journal_title：Biochemical and biophysical research communications

authors： Mathur V,Verma A,Maiti S,Chowdhury S

更新日期：2004-08-06 00:00:00

abstract：:Naringenin is a flavanone compound that alters critical cellular processes such as cell multiplication, glucose uptake, and mitochondrial activity. In this study, we used the social amoeba, Dictyostelium discoideum, as a model system for examining the cellular processes and signaling pathways affected by naringenin. W...

journal_title：Biochemical and biophysical research communications

authors： Misty R,Martinez R,Ali H,Steimle PA

更新日期：2006-06-23 00:00:00

abstract：:The intrinsic GTPase activity of cellular protein p21ras is strongly increased by cytosolic GTPase activating protein (GAP). The activity of this enzyme has been shown to be inhibited by arachidonic acid. We report here that this inhibition is antagonized by the presence of 1-stearoyl, 2-arachidonoyl glycerol. This ef...

journal_title：Biochemical and biophysical research communications

authors： Homayoun P,Stacey DW

更新日期：1993-08-16 00:00:00

abstract：:A synthetic inhibitor of calpain protects rat erythrocyte membrane-associated cytoskeletal proteins from proteolytic degradation (IC50 = 1 microM) which occurs when the cells are rendered permeable to Ca++. Leupeptin, a naturally occurring inhibitor of the enzyme, does not afford any protection at concentrations up to...

journal_title：Biochemical and biophysical research communications

authors： Mehdi S,Angelastro MR,Wiseman JS,Bey P

更新日期：1988-12-30 00:00:00

abstract：:L-amino acids represent the most common amino acid form, most notably as protein residues, whereas D-amino acids, despite their rare occurrence, play significant roles in many biological processes. Amino acid racemases are enzymes that catalyze the interconversion of L- and/or D-amino acids. McyF is a pyridoxal 5'-pho...

journal_title：Biochemical and biophysical research communications

authors： Cao DD,Zhang CP,Zhou K,Jiang YL,Tan XF,Xie J,Ren YM,Chen Y,Zhou CZ,Hou WT

更新日期：2019-07-05 00:00:00

abstract：:The detergent (Triton X-100, 4°C)-resistant membrane (DRM)-associated membrane proteins stomatin, sorcin, and synexin (anexin VII) exposed on the cytoplasmic side of membrane were investigated for their lateral distribution in relation to induced ganglioside(M1) (GM1) raft patches in flat (discocytic) and curved (echi...

journal_title：Biochemical and biophysical research communications

authors： Mrówczyńska L,Salzer U,Perutková S,Iglič A,Hägerstrand H

更新日期：2010-10-22 00:00:00

abstract：:The effects of phenylalanine and tetrahydrobiopterin on the limited proteolysis of rat liver phenylalanine hydroxylase by chymotrypsin have been examined. The presence of tetrahydrobiopterin inhibits the proteolytic activation of native phenylalanine hydroxylase. In contrast, phenylalanine causes a stimulation of prot...

journal_title：Biochemical and biophysical research communications

authors： Phillips RS,Iwaki M,Kaufman S

更新日期：1983-02-10 00:00:00

abstract：:Force impulse is thought to be generated in muscle when myosin head (S-1), while weakly bound to actin filament, undergoes orientational change to form a strong (rigor) bond with actin. There is ample evidence that this bond involves interaction of 1 myosin head with 1 actin monomer. However, X-ray diffraction data of...

journal_title：Biochemical and biophysical research communications

authors： Andreev OA,Borejdo J

更新日期：1991-05-31 00:00:00