Purification of the yellow fluorescent protein from Vibrio fischeri and identity of the flavin chromophore.

Abstract:

:A low molecular weight protein (approximately 25,000 D) exhibiting a yellow fluorescence emission peaking at approximately 540 nm was isolated from Vibrio fischeri (strain Y-1) and purified to apparent homogeneity. FMN is the chromophore, but it exhibits marked red shifts in both the absorption (lambda max = 380, 460 nm) and the fluorescence emission. When added to purified luciferase from the same strain, which itself catalyzes an emission of blue-green light (lambda max approximately 495 nm), this protein induces a bright yellow luminescence (lambda max approximately 540 nm); this corresponds to the emission of the Y-1 strain in vivo. This yellow bioluminescence emission is thus ascribed to the interaction of these two proteins, and to the excitation of the singlet FMN bound to this fluorescent protein.

authors

Macheroux P,Schmidt KU,Steinerstauch P,Ghisla S,Colepicolo P,Buntic R,Hastings JW

doi

10.1016/0006-291x(87)90696-6

subject

Has Abstract

pub_date

1987-07-15 00:00:00

pages

101-6

issue

1

eissn

0006-291X

issn

1090-2104

pii

0006-291X(87)90696-6

journal_volume

146

pub_type

杂志文章
  • Demonstration of a Ca2+/calmodulin dependent protein kinase cascade in the hog heart.

    abstract::Members of the Ca2+/calmodulin dependent protein kinase (CaMK) family and a CaMK cascade have been identified and well characterized in the brain, but little is known about their equivalents in the heart. Thus only CaMKII and its function have been reported so far. Therefore, we purified and characterized CaMKI and Ca...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1998.9154

    authors: Uemura A,Naito Y,Matsubara T,Hotta N,Hidaka H

    更新日期:1998-08-19 00:00:00

  • A possible role of protein kinase C in signal-induced lysosomal enzyme release.

    abstract::In platelets, activation of protein kinase C and mobilization of Ca2+ were selectively induced by the addition of 1-oleoyl-2-acetyl-glycerol and a low concentration of A23187, respectively (Kaibuchi, K., Takai, Y., Sawamura, M., Hoshijima, M., Fujikura, T. and Nishizuka, Y. (1983) J. Biol. Chem. 258, 6701-6704). Using...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(83)90587-9

    authors: Kajikawa N,Kaibuchi K,Matsubara T,Kikkawa U,Takai Y,Nishizuka Y,Itoh K,Tomioka C

    更新日期:1983-10-31 00:00:00

  • DNA chain unwinding and annealing reactions of lipocortin (annexin) I heterotetramer: regulation by Ca(2+) and Mg(2+).

    abstract::Lipocortin I-S100 (calcyclin) heterotetramer exhibited ATPase activity in the presence of dsDNA but not ssDNA. To demonstrate its helicase activity, an 80-mer polynucleotide complementary to the replication origin of M13mp18 was synthesized, and the oligonucleotide, (dC)(20), was ligated to either its 5'- or 3'- end f...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2002.6422

    authors: Hirata A,Hirata F

    更新日期:2002-02-22 00:00:00

  • Cyclin D1/Cdk4 increases the transcriptional activity of FOXM1c without phosphorylating FOXM1c.

    abstract::Anders et al. (2011) [11] reported that cyclinD1/Cdk4 and cyclinD3/Cdk6 enhance the transcriptional activity of FOXM1c by phosphorylating its TAD. They defined 12 Cdk consensus sites as essential for the activation of FOXM1c by cyclinD1/Cdk4 and cyclinD3/Cdk6 and stated that the 12 Cdk-sites are positioned within the ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2013.01.037

    authors: Wierstra I

    更新日期:2013-02-22 00:00:00

  • Appearance of membrane-bound tyrosine kinase during differentiation of HL-60 leukemia cells by immune interferon and tumor necrosis factor.

    abstract::The effect of immune interferon (IFN-gamma) and recombinant tumor necrosis factor (rTNF-alpha) on cellular differentiation was investigated in human promyelocytic leukemia cell line HL-60. Both IFN-gamma and rTNF-alpha induced the appearance of the monocytic phenotype in a dose- and time-dependent manner as assessed b...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(86)90721-7

    authors: Glazer RI,Chapekar MS,Hartman KD,Knode MC

    更新日期:1986-11-14 00:00:00

  • Myomir dysregulation and reactive oxygen species in aged human satellite cells.

    abstract::Satellite cells that reside on the myofibre surface are crucial for the muscle homeostasis and regeneration. Aging goes along with a less effective regeneration of skeletal muscle tissue mainly due to the decreased myogenic capability of satellite cells. This phenomenon impedes proper maintenance and contributes to th...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2016.03.030

    authors: Di Filippo ES,Mancinelli R,Pietrangelo T,La Rovere RM,Quattrocelli M,Sampaolesi M,Fulle S

    更新日期:2016-04-29 00:00:00

  • Influence of cholesterol and ergosterol on membrane dynamics: a fluorescence approach.

    abstract::Sterols are essential membrane components of eukaryotic cells and are important for membrane organization and function. Cholesterol is the most representative sterol present in higher eukaryotes. It is often found distributed non-randomly in domains or pools in biological and model membranes. Cholesterol-rich function...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.04.118

    authors: Arora A,Raghuraman H,Chattopadhyay A

    更新日期:2004-06-11 00:00:00

  • Intrinsic fluorescence from DNA can be enhanced by metallic particles.

    abstract::High sensitivity detection of DNA is essential for genomics. The intrinsic fluorescence from DNA is very weak and almost all methods for detecting DNA rely on the use of extrinsic fluorescent probes. We show that the intrinsic emission from DNA can be enhanced many-fold by spatial proximity to silver island films. Sil...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2001.5445

    authors: Lakowicz JR,Shen B,Gryczynski Z,D'Auria S,Gryczynski I

    更新日期:2001-09-07 00:00:00

  • The transcription factor MEF2C mediates cardiomyocyte hypertrophy induced by IGF-1 signaling.

    abstract::Myocyte enhancer factor 2C (MEF2C) plays an important role in cardiovascular development and is a key transcription factor for cardiac hypertrophy. Here, we describe MEF2C regulation by insulin-like growth factor-1 (IGF-1) and its role in IGF-1-induced cardiac hypertrophy. We found that IGF-1 addition to cultured rat ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2009.07.147

    authors: Muñoz JP,Collao A,Chiong M,Maldonado C,Adasme T,Carrasco L,Ocaranza P,Bravo R,Gonzalez L,Díaz-Araya G,Hidalgo C,Lavandero S

    更新日期:2009-10-09 00:00:00

  • Ultraviolet light-induced crosslinking of two major phosphoproteins and poly(A)+RNA from free polyribosomes; changes in phosphorylation by inhibitors of transcription and translation.

    abstract::Polyribosomes were isolated without the use of detergents, irradiated with ultraviolet light and labelled in the presence of (gamma-32P) adenosine 5'-triphosphate. Poly(A)+RNA-protein structures separated by chromatography on oligo (dT)-cellulose contained up to 1o crosslinked proteins as shown by SDS-polyacrylamide g...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(86)90918-6

    authors: Schweiger A,Kostka G,Weiss E

    更新日期:1986-04-14 00:00:00

  • Carnosine protects proteins against methylglyoxal-mediated modifications.

    abstract::Methylglyoxal (MG) (pyruvaldehyde) is an endogenous metabolite which is present in increased concentrations in diabetics and implicated in formation of advanced glycosylation end-products (AGEs) and secondary diabetic complications. Carnosine (beta-alanyl-L-histidine) is normally present in long-lived tissues at conce...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1998.8806

    authors: Hipkiss AR,Chana H

    更新日期:1998-07-09 00:00:00

  • Cloning, expression, and in vitro activity of human endostatin.

    abstract::Endostatin, a 20 kDa C-terminal fragment of collagen XVIII, is a specific inhibitor of endothelial cell proliferation and angiogenesis. In the present study, we have expressed human endostatin in a yeast expression system (10 mg/L). The recombinant protein was expressed in a soluble form and purified to homogeneity. I...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1999.0595

    authors: Dhanabal M,Volk R,Ramchandran R,Simons M,Sukhatme VP

    更新日期:1999-05-10 00:00:00

  • Evidence for constitutive dimerization of niacin receptor subtypes.

    abstract::The recently deorphanized niacin receptor subtypes NIACR1 (GPR109A) and NIACR2 (GPR109B) play an essential role in the regulation of metabolic processes and immune reactions. Both receptors belong to the G-protein-coupled receptor (GPCR) family, whose members have traditionally been treated as monomeric entities, but ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2010.04.011

    authors: Mandrika I,Petrovska R,Klovins J

    更新日期:2010-04-30 00:00:00

  • Gene expression of growth and differentiation factors-5, -6, and -7 in developing bovine tooth at the root forming stage.

    abstract::Growth and differentiation factors (GDF)-5, -6, and -7 are members of the bone morphogenetic protein (BMP) family. Previous studies suggest their importance in bone development and in tendon/ligament morphogenesis. The cells of the dental attachment apparatus, cementum, periodontal ligament, and alveolar bone proper a...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1998.8213

    authors: Morotome Y,Goseki-Sone M,Ishikawa I,Oida S

    更新日期:1998-03-06 00:00:00

  • Analysis of Ras-dependent signals that prevent caspase-3 activation and apoptosis induced by cytokine deprivation in hematopoietic cells.

    abstract::In hematopoietic cells, Ras has been implicated in signaling pathways that prevent apoptosis triggered by deprivation of cytokines, such as interleukin-3 (IL-3). However, the mechanism whereby Ras suppresses cell death remains incompletely understood. We have investigated the role of Ras in IL-3 signal transduction by...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1999.1955

    authors: Terada K,Kaziro Y,Satoh T

    更新日期:2000-01-07 00:00:00

  • Nitric oxide mediates tightening of the endothelial barrier by ascorbic acid.

    abstract::Vitamin C, or ascorbic acid, decreases paracellular endothelial permeability in a process that requires rearrangement of the actin cytoskeleton. To define the proximal mechanism of this effect, we tested whether it might involve enhanced generation and/or sparing of nitric oxide (NO) by the vitamin. EA.hy926 endotheli...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2010.12.046

    authors: May JM,Qu ZC

    更新日期:2011-01-14 00:00:00

  • Unusual biphasic melting behaviour of Rhodotorula gracilis DNA.

    abstract::Ultraviolet, fluorescence and CD spectral analysis suggested unusual structural features of Rhodotorula gracilis ATCC 90950 DNA. R. gracilis DNA exhibited 13% hyperchromicity at 260 nm as against 26% shown by calf thymus DNA. The biphasic melting curve, one phase between 88-92 degrees C and the other between 92-97 deg...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1995.1907

    authors: Vishnuvardhan D,Divakar S,Joseph R

    更新日期:1995-06-26 00:00:00

  • Feedback-type inhibition of activity of 3-hydroxy-3-methylglutaryl coenzyme a reductase by ubiquinone.

    abstract::Accompanying the decrease in serum cholesterol and increase in concentration of ubiquinone in liver and its microsomes, the activity, but not the protein, of HMG-CoA reductase decreased in ubiquinone-supplemented rats. A soluble 58-kDa preparation of HMG-CoA reductase was partially inhibited on addition of ubiquinone ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(05)80021-x

    authors: Omkumar RV,Gaikwad AS,Ramasarma T

    更新日期:1992-05-15 00:00:00

  • Localization of hydrophobic sites in calmodulin and skeletal muscle troponin C studied using tryptic fragments: a simple method of their preparation.

    abstract::The exposure of hydrophobic sites on calmodulin, skeletal muscle troponin C and their tryptic fragments was investigated using Phenyl-Sepharose chromatography. A strong binding of both proteins and their fragments corresponding to the NH2-terminal halves of polypeptide chain of respective proteins in the presence of c...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(83)90972-5

    authors: Brzeska H,Szynkiewicz J,Drabikowski W

    更新日期:1983-08-30 00:00:00

  • Chemically induced Parkinson's disease. II: Intermediates in the oxidation and reduction reactions of the 1-methyl-4-phenyl-2,3-dihydropyridinium ion and its deprotonated form.

    abstract::The one-electron reduction product of 1-methyl-4-phenyl-2,3-dihydropyridinium ion has been generated by pulse radiolysis and its absorption spectrum recorded. This radical was found to decay by second-order kinetics (2k = 9.5 x 10(8) M-1 s-1) to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and 1-methyl-4-phenyl-2,3-di...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(89)80177-9

    authors: Chacón JN,Chedekel MR,Land EJ,Truscott TG

    更新日期:1989-01-16 00:00:00

  • Covalent conjugation of Groucho with SUMO-1 modulates its corepressor activity.

    abstract::Groucho is a corepressor that forms a macromolecular complex for its corepressor activity, in which HDAC1 is an essential component for the modulation of chromatin structure and transcriptional repression of target genes. Here, we show that Groucho is covalently conjugated with small ubiquitin-related modifier-1 (SUMO...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.12.043

    authors: Ahn JW,Lee YA,Ahn JH,Choi CY

    更新日期:2009-01-30 00:00:00

  • The relation between two molecular species of P-450 in adult testis and 17 alpha-hydroxylase and 17,20-lyase activities.

    abstract::Two P-450s from adult pig testis were purified to specific contents of 11.2 and 12.0 nmol P-450/mg protein and shown to have minimum molecular weights of 45,000 and 46,000, respectively. The absorption spectra were typical of P-450s. The P-450s were separated from the two fractions by CM-C50 Sephadex column chromatogr...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1996.0094

    authors: Kuwada M,Takizawa N,Sone Y

    更新日期:1996-01-17 00:00:00

  • Nonideality of water-hexafluoropropanol mixtures as studied by X-ray small angle scattering.

    abstract::Strong temperature dependent low angle X-ray scattering by trifluoroethanol- and hexafluoro-2-propanol-water mixtures was observed in conditions commonly used in NMR work on peptides. At least two types of molecular effects may contribute to the observed scattering: formation of clathrate hydrate-like aggregates of al...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1995.2889

    authors: Kuprin S,Gräslund A,Ehrenberg A,Koch MH

    更新日期:1995-12-26 00:00:00

  • Proteinase B is, indeed, not required for chitin synthetase 1 function in Saccharomyces cerevisiae.

    abstract::Previous genetic evidence led to the conclusion that proteinase B of yeast was not involved in the function of chitin synthetase 1 (Chs1), based on the demonstration of normal septum formation, cell division and chitin deposition in mutants devoid of the proteinase (Zubenko, G.S., Mitchell, A.P., and Jones, E.W. (1979...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(91)90506-3

    authors: Silverman SJ,Shaw JA,Cabib E

    更新日期:1991-01-15 00:00:00

  • Intra blood-cerebrospinal fluid-barrier detection of hepatitis B virus.

    abstract::Hepatitis B, a major public health concern worldwide, is caused by hepatitis type B virus, a hepdnavirus that infects only human and certain nonhuman primates, and replicates strictly in hepatocytes. By using the techniques of slot and Southern blot DNA hybridization, and electron microscopy, the presence of HBV was i...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(87)90550-x

    authors: Pao CC,Wu SY,Hung IJ,Ng KT,Liaw YF,Lo SJ

    更新日期:1987-07-31 00:00:00

  • The slow-binding inhibition of cathepsin K by its propeptide.

    abstract::A peptide corresponding to the full-length proregion (amino acids 16-114) of human cathepsin K was expressed and purified from Escherichia coli. This recombinant propeptide was investigated for its ability to inhibit the activity of three cysteine proteinases: cathepsins K, L, and B. Kinetic studies showed the propept...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2000.3553

    authors: Billington CJ,Mason P,Magny MC,Mort JS

    更新日期:2000-10-05 00:00:00

  • Hepatic CD147 knockout modulates liver steatosis and up-regulates autophagy in high-fat-diet-induced NAFLD mice.

    abstract::Nonalcoholic fatty liver disease (NAFLD) represents a global health problem. Impaired autophagy has been implicated in the pathogenesis of NAFLD, and CD147 is recognized to regulate lipid metabolism in a variety of cell types. This study was initiated with the aim to identify molecular makers expressed in hepatocytes ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2020.01.164

    authors: Lou J,Li C,Li ZS,Zhang T,Chen ZN,Bian H

    更新日期:2020-04-16 00:00:00

  • Hypertonic induction of COX2 expression requires TonEBP/NFAT5 in renal epithelial cells.

    abstract::TonEBP/NFAT5 transcription factor is a master regulator of genes involved in osmoprotection. Cyclooxygenase 2 (COX2) has been reported to be a cytoprotective molecule in the inner renal medulla, where cells are physiologically exposed to the highest osmolality of the body. Our aim was to study whether COX2 expression ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.12.189

    authors: Favale NO,Casali CI,Lepera LG,Pescio LG,Fernández-Tome MC

    更新日期:2009-04-10 00:00:00

  • Effects of daunorubicin on ganglioside expression and neuronal differentiation of mouse embryonic stem cells.

    abstract::Gangliosides are implicated in neuronal development processes. The regulation of ganglioside levels is closely related to the induction of neuronal cell differentiation. In this study, the relationship between ganglioside expression and neuronal cell development was investigated using an in vitro model of neural diffe...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2007.07.142

    authors: Lee DH,Koo DB,Ko K,Ko K,Kim SM,Jung JU,Ryu JS,Jin JW,Yang HJ,Do SI,Jung KY,Choo YK

    更新日期:2007-10-19 00:00:00

  • Folate-modified PLGA nanoparticles for tumor-targeted delivery of pheophorbide a in vivo.

    abstract::Targeted drug delivery has been an important issue for tumor therapy including photodynamic therapy (PDT). The purpose of our study is to increase the targeting efficiency of photosensitizer (PS) using folate-modified nanoparticles (NPs) to tumor site in vivo. Folate receptor is over-expressed on the surface of many h...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2018.03.013

    authors: Son J,Yang SM,Yi G,Roh YJ,Park H,Park JM,Choi MG,Koo H

    更新日期:2018-04-06 00:00:00