Helix stabilization in the C-terminal peptide of chicken riboflavin carrier protein enhances immunogenicity and prolongs contraceptive potential as an epitope-based vaccine in female rats.

Abstract:

:Earlier investigations have shown that (a) antibodies against a carrier-coupled 20-residue synthetic peptide (C-20), (200)HACQKKLLKFEALQQEEGEE(219), corresponding to the C-terminal partially helical sequence of chicken riboflavin carrier protein (RCP; 219 AA) curtail pregnancy in mammals and (b) helix stabilization by introducing appropriately spaced salt bridges in the flanking sequences of its B-cell epitopic structure enhances RCP antigenicity to peptide antibodies. Among such engineered C-20 analogs, HE-20 (HAEQKKLLKFEALEQEKGKE) exhibited maximum helical propensity. Since C-20 per se, i.e., without carrier conjugation, elicits RCP-reactive neutralizing antibodies in rodents, we mapped its T-cell epitope which overlaps its B-cell epitope, both of which remain unmodified in HE-20. Comparative evaluation of immunogenicity of the two epitope-based peptide vaccines showed that HE-20 was far superior to C-20 in generating RCP-reactive antibodies in terms of both affinity and titer. With regard to bioefficacy, passive immunoneutralization of RCP in pregnant rats by administering purified IgG from either of the antipeptide sera terminated pregnancy. Similarly, active immunization of fertile female rats with the individual peptide analogs curtailed pregnancy. However, HE-20 was more efficient in eliciting higher affinity, longer-lasting, RCP-crossreactive antibodies with consequently more prolonged immunocontraceptive efficacy.

authors

Subramanian S,Karande AA,Adiga PR

doi

10.1006/bbrc.2001.5571

subject

Has Abstract

pub_date

2001-09-14 00:00:00

pages

236-43

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006291X01955718

journal_volume

287

pub_type

杂志文章
  • Conformational dynamics of loops L11 and L12 of kinesin as revealed by spin-labeling EPR.

    abstract::The EPR spectra of the spin labels attached to loops L11 and L12 of kinesin were resolved into slow (rotational correlation time, tau=10-45 ns) and fast (tau=2 ns) components. The fraction of the slow component increased considerably when kinesin was complexed with a microtubule (MT). On MT binding and in the presence...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2007.10.043

    authors: Yamada MD,Maruta S,Yasuda S,Kondo K,Maeda H,Arata T

    更新日期:2007-12-21 00:00:00

  • Glycogen-storage disease type II (acid maltase deficiency): identification of a novel small deletion (delCC482+483) in French patients.

    abstract::Glycogen-storage disease type II (GSD II, acid maltase deficiency, Pompe's disease) is caused by defects in the lysosomal acid alpha-glucosidase (GAA) gene. Clinically, patients with the severe infantile form of GSD II have muscle weakness and cardiomyopathy eventually leading to death before the age of two years. Pat...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1997.6749

    authors: Nicolino M,Puech JP,Letourneur F,Fardeau M,Kahn A,Poenaru L

    更新日期:1997-06-09 00:00:00

  • Zyxin is up-regulated during megakaryocytic differentiation of human UT-7/c-mpl cells.

    abstract::To characterize genes involved in megakaryocytic commitment, we compared expression profiles of bipotent cells (UT-7/c-mpl) with those of the same cells induced to differentiate towards megakaryopoiesis in the presence of TPO. Using cDNA arrays, we showed that 12 out of 2260 genes changed their expression level after ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.04.047

    authors: Decraene C,Garçon L,Lacout C,Sabri S,Auffray C,Vainchenker W,Duménil D,Piétu G,Svinartchuk F

    更新日期:2004-05-28 00:00:00

  • Local database and the search program for proteomic analysis of sperm proteins in the ascidian Ciona intestinalis.

    abstract::Separation of proteins by two-dimensional electrophoresis and following mass spectrometry (MS) is now a conventional technique for proteomic analysis. For proteomic analysis of a certain tissue with a limited information of primary structures of proteins, we have developed an analytical system for peptide mass fingerp...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.05.118

    authors: Hozumi A,Satouh Y,Ishibe D,Kaizu M,Konno A,Ushimaru Y,Toda T,Inaba K

    更新日期:2004-07-09 00:00:00

  • Intranuclear interactomic inhibition of FoxP3 suppresses functions of Treg cells.

    abstract::Regulatory T cells (Treg cells) are crucial for the maintenance of immunological tolerance, and it has been reported that Treg cells are enriched within the tumor micro-environment for immune evasion due to their immunosuppressive functions. To inhibit Treg cells functions, FoxP3, a lineage-specific transcription fact...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2014.06.141

    authors: Park JH,Ko JS,Shin Y,Cho JY,Oh HA,Bothwell AL,Lee SK

    更新日期:2014-08-15 00:00:00

  • Maternally-derived antibody to fibroblast growth factor-23 reduced dietary phosphate requirements in growing chicks.

    abstract::Phosphate in manure of monogastric animals pollutes the environment if improperly applied to soil. Strategies that reduce phosphate inputs into animal production systems reduce environmental pollution. Using a novel vaccine to fibroblast growth factor-23 (FGF-23), we induced neutralizing antibodies that reduced the ph...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2012.03.063

    authors: Bobeck EA,Burgess KS,Jarmes TR,Piccione ML,Cook ME

    更新日期:2012-04-13 00:00:00

  • The l-isoform but not d-isoforms of a JNK inhibitory peptide protects pancreatic beta-cells.

    abstract::The activation of c-jun N-terminal kinase (JNK) in pancreatic islets is associated with impaired function and viability, and JNK inhibitory peptides (JNKIs) are cytoprotective. In particular, l-isoforms of JNKIs were shown to improve islets viability, while the d-retroinverso isoform of JNKI (RI-JNKI), with a higher t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2006.12.186

    authors: Fornoni A,Cobianchi L,Sanabria NY,Pileggi A,Molano RD,Ichii H,Rosero S,Inverardi L,Ricordi C,Pastori RL

    更新日期:2007-03-02 00:00:00

  • Metformin, resveratrol, and exendin-4 inhibit high phosphate-induced vascular calcification via AMPK-RANKL signaling.

    abstract::Vascular calcification increases the risk of developing cardiovascular disease, and it is closely associated with metabolic disorders such as diabetes mellitus and non-alcoholic fatty liver disease. We investigated whether the activators of AMP-activated protein kinase (AMPK), metformin, resveratrol, and exendin-4, im...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2020.07.136

    authors: Lee J,Hong SW,Kim MJ,Kwon H,Park SE,Rhee EJ,Lee WY

    更新日期:2020-09-17 00:00:00

  • LncRNA snaR upregulates GRB2-associated binding protein 2 and promotes proliferation of ovarian carcinoma cells.

    abstract:BACKGROUND:The functionality of lncRNA snaR has only been characterized in breast cancer and colon cancer. The aim of the current study is to explore the involvement of lncRNA snaR in ovarian carcinoma (OC). MATERIALS AND METHODS:Expression of lncRNA snaR and GRB2-associated binding protein 2 (GAB2) in plasma of both ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2018.07.152

    authors: Huang Y,Hu Y,Jin Z,Shen Z

    更新日期:2018-09-10 00:00:00

  • α-Melanocyte stimulating hormone attenuates dexamethasone-induced osteoblast damages through activating melanocortin receptor 4-SphK1 signaling.

    abstract::Long-term glucocorticoid (GC) usage may cause non-traumatic femoral head osteonecrosis. Dexamethasone (Dex) is shown to exert potent cytotoxic effect to osteoblasts. Here, we investigated the potential activity of α-melanocyte stimulating hormone (α-MSH) against the process. Our data revealed that pretreatment of α-MS...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2015.11.104

    authors: Guo S,Xie Y,Fan JB,Ji F,Wang S,Fei H

    更新日期:2016-01-08 00:00:00

  • Restoration of nuclear-import failure caused by triple A syndrome and oxidative stress.

    abstract::Triple A syndrome is an autosomal recessive neurological disease, mimicking motor neuron disease, and is caused by mutant ALADIN, a nuclear-pore complex component. We recently discovered that the pathogenesis involved impaired nuclear import of DNA repair proteins, including DNA ligase I and the cerebellar ataxia caus...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.07.088

    authors: Kiriyama T,Hirano M,Asai H,Ikeda M,Furiya Y,Ueno S

    更新日期:2008-10-03 00:00:00

  • Lysosomal tartrate sensitive acid phosphatase deficiency in cells which contain lysosomal "high uptake forms".

    abstract::The catalytic and immunological properties of acid phosphatases (EC 3.1.3.2.) in different tissues were studied. It was demonstrated that high uptake forms of lysosomal enzymes like beta-galactosidase isolated from human platelets and bovine testis are mature enzymes, which have not lost their mannose-6phosphate marke...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(90)92055-5

    authors: Hu P,Parenti G,Keulemans J,Hoogeveen AT

    更新日期:1990-03-16 00:00:00

  • Expression of recombinant rat interleukin-13 (IL-13) and generation of a neutralizing rat IL-13 antiserum.

    abstract::Using baculoviral and bacterial systems, we expressed biologically active recombinant rat IL-13 and generated neutralizing rat IL-13 antiserum. Recombinant rat IL-13 produced by baculovirus-infected insect cells stimulated proliferation of TF-1 premyeloid cell line and induced expression of 15-lipoxygenase mRNA in hum...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1997.6832

    authors: Lakkis FG,Cruet EN,Nassar GM,Badr KF,Pascual DW

    更新日期:1997-06-27 00:00:00

  • AAV-mediated delivery of the transcription factor XBP1s into the striatum reduces mutant Huntingtin aggregation in a mouse model of Huntington's disease.

    abstract::Huntington's disease (HD) is caused by mutations that expand a polyglutamine region in the amino-terminal domain of Huntingtin (Htt), leading to the accumulation of intracellular inclusions and progressive neurodegeneration. Recent reports indicate the engagement of endoplasmic reticulum (ER) stress responses in human...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2012.03.033

    authors: Zuleta A,Vidal RL,Armentano D,Parsons G,Hetz C

    更新日期:2012-04-13 00:00:00

  • Dynamics of oxygen level-driven regulators in modulating autophagy in colorectal cancer cells.

    abstract::Colorectal cancer is a common cancer with metachronous distant metastases still threatening overall survival. Tumor oxygen level influences tumor radiosensitivity in relation to autophagy and apoptosis. The objective of this study is to evaluate the expression and interaction between multiple key regulators in differe...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2019.07.043

    authors: Tam SY,Wu VWC,Law HKW

    更新日期:2019-09-17 00:00:00

  • Bacterial β-glucuronidase alleviates dextran sulfate sodium-induced colitis in mice: A possible crucial new diagnostic and therapeutic target for inflammatory bowel disease.

    abstract:OBJECTIVE:Inflammatory bowel diseases (IBD) including ulcerative colitis and Crohn's disease are devastating diseases of the gut. At present, all the treatments are mainly targeting symptoms like inflammation. The disease remains regarded as incurable, largely due to lacking of knowledge on its etiology. Our previous s...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2019.03.196

    authors: He Y,Yu H,Ge Y,Li X,Jiang M,Liu Y,Li X,Wang Y,Guo M,Qin X,Wang X

    更新日期:2019-05-28 00:00:00

  • Possible involvement of group I mGluRs in neuroprotective effect of theanine.

    abstract::We investigated the molecular mechanism underlying the neuroprotective effect of theanine, a green tea component, using primary cultured rat cortical neurons, focusing on group I metabotropic glutamate receptors (mGluRs). Theanine and a group I mGluR agonist, DHPG, inhibited the delayed death of neurons caused by brie...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.05.143

    authors: Nagasawa K,Aoki H,Yasuda E,Nagai K,Shimohama S,Fujimoto S

    更新日期:2004-07-16 00:00:00

  • Phosphoglycerate mutase has essential arginyl residues.

    abstract::Phosphoglycerate mutase is inactivated by butanedione in borate buffer. Inactivation by 0.13 mM reagent correlates with the modification of one arginyl residue per subunit, and is prevented by either 2, 3-diphosphoglycerate or 3-phosphoglycerate. With 0.50 mM butanedione, inactivation is accompanied by the modificatio...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(76)90218-7

    authors: Borders CL Jr,Wilson BA

    更新日期:1976-12-20 00:00:00

  • Sequencing and characterization of the human thiazide-sensitive Na-Cl cotransporter (SLC12A3) gene promoter.

    abstract::The thiazide-sensitive Na-Cl cotransporter SLC12A3 displays expression restricted to distal convoluted tubule cells where it catalyzes the uptake of sodium and chloride through the apical membrane. We sequenced 1959 bp of the 5' flanking region of human SLC12A3, located the area of transcription initiation, and used d...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2001.4673

    authors: MacKenzie S,Vaitkevicius H,Lockette W

    更新日期:2001-04-13 00:00:00

  • Overexpression of stearoyl-CoA desaturase-1 results in an increase of conjugated linoleic acid (CLA) and n-7 fatty acids in 293 cells.

    abstract::The effect of human SCD1 heterologous expression on cellular fatty acid synthesis was investigated in the current study. The SCD1 gene expression cassette and PGK-neomycin-selectable marker cassette were co-introduced into HEK 293 cells by electroporation, and subsequently, SCD1 expression was evaluated by fatty acid ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2010.06.102

    authors: Wu Z,Li D,Gou K

    更新日期:2010-07-30 00:00:00

  • GPER stabilizes F-actin cytoskeleton and activates TAZ via PLCβ-PKC and Rho/ROCK-LIMK-Cofilin pathway.

    abstract::Actin is a highly abundant cytoskeletal protein that is essential for all eukaryotic cells and participates in many structural and functional roles. It has long been noted that estrogen affects cellular morphology. However, recent studies observed that both estrogen and tamoxifen induce a remarkable cytoskeletal remod...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2019.06.132

    authors: Wang Z,Sun L,Liang S,Liu ZC,Zhao ZY,Yang J,Wang D,Yang DQ

    更新日期:2019-08-27 00:00:00

  • Identification of the AQP8-miR-92a network associated with the aggressive traits of colorectal cancer.

    abstract::Even though earlier reports have revealed that Aquaporin 8 (AQP8) exert essential roles in diverse malignancies, its relationship between specific microRNAs (miRNAs) in regulation of colorectal carcinoma (CRC) progression has never been elaborated. Herein, we proved that AQP8 was downregulated in CRC and high level of...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2020.04.055

    authors: Zhang H,Du WB,Guo XM,Wang LK,Cheng JM,Wei LJ

    更新日期:2020-06-18 00:00:00

  • A new locus for a dominant form of multinodular goiter on 3q26.1-q26.3.

    abstract::A mass screening program for congenital hypothyroidism has markedly improved prognosis of children with congenital hypothyroidism and also revealed several cases with unknown pathogenesis. We here report two independent Japanese multigeneration families with multinodular goiter (MNG) with euthyroidism and with high TS...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2001.4998

    authors: Takahashi T,Nozaki J,Komatsu M,Wada Y,Utsunomiya M,Inoue K,Takada G,Koizumi A

    更新日期:2001-06-15 00:00:00

  • Participation of macrophage membrane rafts in Trypanosoma cruzi invasion process.

    abstract::Membrane rafts are small and dynamic regions enriched in sphingolipids, cholesterol, ganglioside GM1 and protein markers like flotillins, forming the flatter domains or caveolins, which are characterized as stable flask-shape invaginations. We explored whether membrane rafts participate in the entry of Trypanosoma cru...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2007.09.068

    authors: Barrias ES,Dutra JM,De Souza W,Carvalho TM

    更新日期:2007-11-23 00:00:00

  • Three-hybrid strategy reveals a peptide segment that specifically binds to the 3'-untranslated region of NF-IL6 mRNA.

    abstract::The 3'UTR of eukaryotic mRNA is an important regulation region, on which many trans factors act. In recent years, a series of 3'UTRs were shown to have tumor suppressor function, including the 3'UTR of the human nuclear factor for interleukin-6 (NF-IL6 3'UTR). To understand molecular basis for this function, we have t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2000.3425

    authors: He YZ,Liu DG

    更新日期:2000-09-16 00:00:00

  • GnRH neuron type-specific transcriptome analysis by laser captured single-cell microarray in the medaka.

    abstract::Most vertebrates possess at least two gonadotropin-releasing hormone (GnRH) neuron types. To understand the physiological significance of the multiple GnRH systems in the brain, we examined three GnRH neuron type-specific transcriptomes using single-cell microarray analyses in the medaka (Oryzias latipes). A microarra...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2013.05.004

    authors: Moriya S,Ogawa S,Parhar IS

    更新日期:2013-06-14 00:00:00

  • Down-regulation of Barx2 predicts poor survival in colorectal cancer.

    abstract::Human BarH-like homeobox 2 (Barx2), a homeodomain factor of the Bar family, has an important role in controlling the expression of cell adhesion molecules and has been reported in an increasing array of tumor types except colorectal cancer (CRC). The purpose of the current study was to characterize the expression of B...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2016.07.091

    authors: Mi Y,Zhao S,Zhang W,Zhang D,Weng J,Huang K,Sun H,Tang H,Zhang X,Sun X,Peng Z,Wen Y

    更新日期:2016-09-09 00:00:00

  • Inhibition of the induction of nitric oxide synthase by spermine is modulated by aldehyde dehydrogenase.

    abstract::The observation that spermine inhibits the endotoxin (lipopolysaccharide; LPS) induced production of nitric oxide (NO) in macrophages has been ascribed to the conversion of SP to active metabolites by the action of enzymes, such as diamine oxidases, found, for example, in bovine sera. Inhibitory effect is also observe...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1994.2374

    authors: Southan GJ,Szabó C,Thiemermann C

    更新日期:1994-09-30 00:00:00

  • KMT Set7/9 is a new regulator of Sam68 STAR-protein.

    abstract::Lysine-specific methyltransferase Set7/9 (KMT7) belongs to the SET domain family of proteins. Besides the SET domain, Set7/9 also contains a so-called MORN (Membrane Occupation and Recognition Nexus) domain whose function in high eukaryotes is largely unknown. Set7/9 has been shown to specifically methylate both histo...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2020.03.017

    authors: Vasileva E,Shuvalov O,Petukhov A,Fedorova O,Daks A,Nader R,Barlev N

    更新日期:2020-05-14 00:00:00

  • High sensitivity of tubifex for ultraviolet-B.

    abstract::Phytoplankton and zooplankton serve as major food material for primary and secondary consumers in the marine food web. Although phytoplankton are primary targets to UV radiation damaging effects, we have demonstrated that stratospheric ozone depletion will affect zooplankton to contribute to inhibition in biomass prod...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1997.6055

    authors: Soni AK,Joshi PC

    更新日期:1997-02-24 00:00:00