Abstract:
:Zebrafish embryos can be obtained for research purposes in large numbers at low cost and embryos develop externally in limited space, making them highly suitable for high-throughput cancer studies and drug screens. Non-invasive live imaging of various processes within the larvae is possible due to their transparency during development, and a multitude of available fluorescent transgenic reporter lines.To perform high-throughput studies, handling large amounts of embryos and larvae is required. With such high number of individuals, even minute tasks may become time-consuming and arduous. In this chapter, an overview is given of the developments in the automation of various steps of large scale zebrafish cancer research for discovering important cancer pathways and drugs for the treatment of human disease. The focus lies on various tools developed for cancer cell implantation, embryo handling and sorting, microfluidic systems for imaging and drug treatment, and image acquisition and analysis. Examples will be given of employment of these technologies within the fields of toxicology research and cancer research.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
van der Ent W,Veneman WJ,Groenewoud A,Chen L,Tulotta C,Hogendoorn PC,Spaink HP,Snaar-Jagalska BEdoi
10.1007/978-3-319-30654-4_14subject
Has Abstractpub_date
2016-01-01 00:00:00pages
315-32eissn
0065-2598issn
2214-8019journal_volume
916pub_type
杂志文章,评审abstract::In the visible/NIR (600-900 nm) three different redox centres are potentially detectable in vivo in mitochondrial cytochrome c oxidase: haem a (605nm), the binuclear haem a3/CuB centre (655 nm) and CuA (830 nm). In this paper we report changes in the steady state reduction of these centres following increases in the r...
journal_title:Advances in experimental medicine and biology
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journal_title:Advances in experimental medicine and biology
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abstract::Prevalence of zoonotic Mycobacterium bovis (bTB) disease in human population is underreported from the North of Pakistan. Here, we report on the proportion of human bTB disease among the overall TB patients, drug resistance pattern of bTB isolates, and knowledge, attitude, and practices (KAP)-based analysis of bTB dis...
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journal_title:Advances in experimental medicine and biology
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pub_type: 杂志文章,评审
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
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更新日期:2014-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 历史文章,杂志文章
doi:10.1007/978-1-4614-2098-9_2
更新日期:2012-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/5584_2016_122
更新日期:2017-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4615-1891-4_27
更新日期:1995-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4615-9110-8_9
更新日期:1979-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:
更新日期:1995-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-981-10-1503-8_10
更新日期:2016-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4615-3806-6_34
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
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pub_type: 杂志文章,评审
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abstract::Human milk samples contain a particularly rich collection of oligosaccharides compared with other milk samples. The synthesis of these molecules should depend on the expression of glycosyltransferases and the presence of sugar nucleotides in lactating mammary glands. We set out to produce transgenic animals expressing...
journal_title:Advances in experimental medicine and biology
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更新日期:2001-01-01 00:00:00
abstract::We identified a 45 kDa Ca(2+)-dependent Lf binding protein on rat hepatocytes. Dithiobis(sulfosuccimidylproprionate) (DTSSP)-crosslinked 125I-Lf to a 45 kDa adduct in a Ca(2+)-dependent manner on intact cells. The 125I-labeled crosslinked complexes were absent when either surface-bound 125I-Lf was stripped prior to cr...
journal_title:Advances in experimental medicine and biology
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更新日期:1998-01-01 00:00:00
abstract::There are as yet no markers known to predict the course of sarcoidosis. High resolution computed tomography (HRCT) is a tool that enables to visualize subtle parenchymal opacities in the lungs. Therefore, the aim of this study was to assess the prognostic role of HRCT at Stage I sarcoidosis. Fifty one patients (28 mal...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
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更新日期:2013-01-01 00:00:00
abstract::Chronic nonmalignant pulmonary diseases impose a heavy burden on patients, generate health-care costs, and contribute to poor health-related quality of life. It has been found that a wide range of factors negatively affects quality of life, but the role of acceptance of illness needs to be further investigated. The ai...
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更新日期:2019-01-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2019-01-01 00:00:00
abstract::Immune checkpoint inhibitors (ICIs) have shown significant benefit in cancer patients, but are associated with immune-related adverse events (irAEs), that can affect the gastrointestinal tract resulting in diarrhea and colitis. IrAEs range from mild self-limiting to severe life-threatening disease, which potentially l...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-3-030-02505-2_7
更新日期:2018-01-01 00:00:00
abstract::A major challenge in proteomic biomarker discovery and validation for psychiatric diseases is the inherent biological complexity underlying these conditions. There are also many technical issues which hinder this process such as the lack of standardization in sampling, processing and storage of bio-samples in preclini...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
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更新日期:2017-01-01 00:00:00