Abstract:
:Tumour necrosis factor alpha (TNF-α) is one kind of cytokines which is related to inflammation and lipid metabolism. TNF-α cDNA was cloned from the liver of blunt snout bream (Megalobrama amblycephala) through real-time polymerase chain reaction (PCR) and rapid amplification of cDNA ends (RACE) methods. The full-length cDNA of TNF-α covered 1467 bp, with an open reading frame (ORF) of 723 bp, which encodes 240 amino acids. It possessed the TNF family signature IIIPDDGIYFVYSQ. After the lipopolysaccharide (LPS) challenge test, a graded tissue-specific expression pattern of TNF-α was observed and there was high expression abundance in the kidney, brain and liver. After 8 weeks feeding trial, liver samples, two groups fed with 6% and 11% lipid levels, were collected. The results showed that, for fish fed with high-fat diet, the triglyceride of serum and lipid content of liver were elevated. Furthermore, TNF-α and peroxisome proliferator-activated receptors (PPARα, β) mRNA expression of fish fed 11% lipid diet were significantly up-regulated (p < 0.05). Lipoprotein lipase (LPL) and PPARγ mRNA expression of fish fed 11% lipid lever diet were significantly decreased compared to those of fish fed 6% (p < 0.05). The differences between the various expression of related genes in the high and low fat groups demonstrated that TNF-α played a key role in lipid metabolism, which may have an influence on fat metabolism through reducing fat synthesis and strengthening the β-oxidation of fatty acid. These discrepancies warrant further research.
journal_name
J Appl Genetjournal_title
Journal of applied geneticsauthors
Zhou M,Mi HF,Liu WB,Wu YY,Wang KZ,Jiang GZdoi
10.1007/s13353-017-0390-9subject
Has Abstractpub_date
2017-08-01 00:00:00pages
381-391issue
3eissn
1234-1983issn
2190-3883pii
10.1007/s13353-017-0390-9journal_volume
58pub_type
杂志文章abstract::The long QT syndrome (LQTS) is an inherited cardiac arrhythmia that may lead to sudden death in the absence of structural heart disease. Mutations in the cardiac potassium and sodium channel genes can be found in approximately 70 percent of patients with a highly probable clinical diagnosis. In this study, we aimed to...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/BF03208882
更新日期:2010-01-01 00:00:00
abstract::The insulin-like growth factor 2 gene (IGF2) has been described in several studies as a candidate gene for meat efficiency in pigs. IGF2 is a member of the growth factors family and has an effect on development of muscle tissue. The effect of IGF2 gene polymorphism on meat efficiency was analysed in a population of 12...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Based on segregation distortion of simple sequence repeat (SSR) molecular markers, we detected a significant quantitative trait loci (QTL) for pre-harvest sprouting (PHS) tolerance on the short arm of chromosome 2D (2DS) in the extremely susceptible population of F2 progeny generated from the cross of PHS tolerant syn...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/BF03195631
更新日期:2008-01-01 00:00:00
abstract::Interleukin-15 (IL-15) is a cytokine that has been proposed to modulate skeletal muscle and adipose tissue mass. In the present study, an F(2) resource population of Gushi chickens crossed with Anka broilers was used to investigate the genetic effects of the chicken IL-15 gene. Two single nucleotide polymorphisms (SNP...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-012-0111-3
更新日期:2012-11-01 00:00:00
abstract::Hybrids derived from wheat (Triticum aestivum L.) × rye (Secale cereale L.) have been widely studied because of their important roles in wheat cultivar improvement. Repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 are usually used as probes in fluorescence in situ hybridization (FISH) analysis of...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-014-0215-z
更新日期:2014-08-01 00:00:00
abstract::The aim of this study was to evaluate phenotypic variation of R0 androgenic plants obtained from four seed sources and donor plants by anther culture. Several morphological traits (leaf size, petiole length, leaf division, cortex colour) and the range of diversity were evaluated. There was large variation in all trait...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::In addition to their normal developmental processes, plants have evolved complex genetic and epigenetic regulatory mechanisms to cope with various environmental stresses. It has been shown that both DNA methylation and histone modifications are involved in DNA damage response to various types of stresses. In this stud...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-014-0226-9
更新日期:2014-11-01 00:00:00
abstract::Hemp (Cannabis sativa L.) is a species considered as having one of the most complicated mechanisms of sex determination. Peroxidase and esterase isoenzymes in leaves of the two sexual phenotypes of hemp were studied. Significant differences in isoperoxidase and isoesterase patterns were found between male and female p...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Laminopathies, a group of heterogeneous disorders associated with lamin A/C gene (LMNA) mutations, encompass a wide spectrum of clinical phenotypes, which may present as separate disease or as overlapping syndromes. We describe a 35-year-old female in whom a novel sporadic heterozygous mutation c.1001_1003delGCC (p.Se...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-016-0365-2
更新日期:2017-02-01 00:00:00
abstract::Pre-harvest sprouting (PHS) and seed longevity (SL) are complex biological processes of major importance for agricultural production. In the present study, a recombinant inbred line (RIL) population derived from a cross between the German winter wheat (Triticum aestivum L.) cultivars History and Rubens was used to ide...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-017-0422-5
更新日期:2018-02-01 00:00:00
abstract::The genetic determination of variability of barley doubled haploid (DH) lines in regard of their susceptibility to Fusarium head blight caused by Fusarium culmorum was studied. The susceptibility was evaluated in 3-year field experiment on the basis of reduction in yield traits and myotoxin accumulation in infected ke...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::This study aimed to determine the frequency of associated urological abnormalities in children with unilateral renal agenesis (RA) or multicystic dysplastic kidney (MCDK). In total, 38 children (10 girls, 28 boys) were studied: 21 with RA and 17 with MCDK. In 14 children (37%) anomalies of the urinary tract were suspe...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/BF03194618
更新日期:2006-01-01 00:00:00
abstract::Group-1 homoelog genes in wheat genomes encode storage proteins and are the major determinants of wheat product properties. Consequently, understanding the genetic diversity of group-1 homoelogs and genes encoding storage proteins, especially the low-molecular-weight glutenins (LMW-GSs), within wheat landrace genomes ...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-020-00564-6
更新日期:2020-09-01 00:00:00
abstract::Streptomyces ghanaensis ATCC14672 is the producer of phosphoglycolipid antibiotics moenomycins that for almost 40 years were used worldwide as an animal feed additive. As the use of moenomycins narrows down (due to bans in the EU and some other countries), it opens the opportunity to develop much-needed antibiotics ag...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-015-0283-8
更新日期:2015-11-01 00:00:00
abstract::The quality of wheat depends on a large complex of genes and environmental factors. The objective of this study was to identify quantitative trait loci controlling technological quality traits and their stability across environments, and to assess the impact of interaction between alleles at loci Glu-1 and Glu-3 on gr...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-016-0362-5
更新日期:2017-02-01 00:00:00
abstract::Fast and efficient DNA fingerprinting of crop cultivars and individuals is frequently used in both theoretical population genetics and in practical breeding. Numerous DNA marker technologies exist and the ratio of speed, cost and accuracy are of importance. Therefore even in species where highly accurate and polymorph...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/BF03194627
更新日期:2006-01-01 00:00:00
abstract::A simplified AFLP method, based on methylation-sensitive Alw44I restriction endonuclease, has been developed and evaluated for fingerprinting 15 wheat cultivars. The selected germplasms represented groups of spring and winter wheats with and without the 1BL.1RS translocation. Ten selective primers yielded 57 markers, ...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::Nearly all bacterial species, including pathogens, have the ability to form biofilms. Biofilms are defined as structured ecosystems in which microbes are attached to surfaces and embedded in a matrix composed of polysaccharides, eDNA, and proteins, and their development is a multistep process. Bacterial biofilms const...
journal_title:Journal of applied genetics
pub_type: 杂志文章,评审
doi:10.1007/s13353-015-0309-2
更新日期:2016-05-01 00:00:00
abstract::Observations of a specific rDNA locus behaviour during the cell cycle were made by fluorescent in situ hybridisation (FISH) in 12 Lupinus species. Due to the pattern of chromatin de-condensation in that locus, the number of relevant sites in interphase nuclei was twice as high as the number of signals on metaphase chr...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::Soluble glycerol-3-phosphate dehydrogenase 1 (GPD1, EC 1.1.1.8) plays important roles in the synthesis of triacylglycerol and in the glycerol-3-phosphate shutter. Though GPD1 is expressed in most adult tissues, little is known about the regulation of its expression. In this study, we analyzed the characters, organizat...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-011-0050-4
更新日期:2011-11-01 00:00:00
abstract::Spring wheat nursery accessions, including 18 spring wheat lines derived in CIMMYT, Mexico, and 12 spring wheat cultivars bred in Poland, along with cultivars Frontana and Sumai 3 as resistant controls, were examined for resistance to leaf rust under field conditions. Multipathotype tests with 16 different pathogen is...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::In this study we have developed protocols for induced triploidy and gynogenesis of Senegalese sole (Solea senegalensis), a promising flatfish species for marine aquaculture, in order to: 1) identify the sex-determination mechanism; and 2) to improve its production by generating a) sterile fish, avoiding problems relat...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-014-0233-x
更新日期:2015-02-01 00:00:00
abstract::Deoxyguanosine kinase deficiency (dGK) is a frequent cause of the hepatocerebral form of mitochondrial depletion syndrome (MDS). A group of 28 infants with severe progressive liver failure of unknown cause was recruited for post mortem search for deoxyguanosine kinase (DGUOK) gene mutations. Four affected patients (14...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-010-0008-y
更新日期:2011-02-01 00:00:00
abstract::Phenylketonuria (PKU) is a metabolic disease caused by mutations in the phenylalanine hydroxylase (PAH) gene. Although the PAH genotype remains the main determinant of PKU phenotype severity, genotype-phenotype inconsistencies have been reported. In this study, we focused on unanalysed sequences in non-coding PAH gene...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-016-0359-0
更新日期:2017-02-01 00:00:00
abstract::The mode of replication and organisation of bacterial genomes impose asymmetry on their nucleotide composition. The asymmetry is seen in coding and non-coding sequences and is reflected in the amino acid composition of proteins. The mechanisms generating asymmetry include: unequal mutation rates connected with replica...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::A linkage map of garden pea was constructed on the basis of 114 plants (F2 generation) derived from a cross combination Wt10245 x Wt11238. The map, consisting of 204 morphological, isozyme, AFLP, ISSR, STS, CAPS and RAPD markers, was used for interval mapping of quantitative trait loci (QTLs) controlling seed number, ...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::The aim of this study was to assess the frequencies of three hemochromatosis gene (HFE) mutations in ethnic Roma/Gypsies in Slovakia. A cohort of 367 individuals representing general population and not preselected for health status was genotyped by TaqMan real-time PCR assay for C282Y, H63D and S65C mutations in HFE g...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-012-0088-y
更新日期:2012-05-01 00:00:00
abstract::Pollen proteases were discovered over 100 years ago, whereas the enzymes from female tissues have been used since the Roman era in simple biotechnological processes. In the last decade a great progress has been made in studies on plant proteases, including those from the generative organs. This paper reviews reports p...
journal_title:Journal of applied genetics
pub_type: 杂志文章,评审
doi:
更新日期:2005-01-01 00:00:00
abstract::The mechanisms of root hair formation have been studied extensively in Arabidopsis but knowledge about these processes in monocot species is still limited, especially in relation to the proteome level. The aim of this study was to identify the proteins that are involved in the initiation and the early stage of root ha...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-012-0105-1
更新日期:2012-11-01 00:00:00
abstract::Myogenic factor 5 (myf-5) is the product of the MYF5 gene, belonging to the MyoD family. This transcription factor participates in the control of myogenesis. We identified 3 new mutations in the promoter region of the gene: A65C, C580T and C613T. The aim of this study was to evaluate the influence of the A65C transver...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/BF03194600
更新日期:2006-01-01 00:00:00