Abstract：
:Streptomyces ghanaensis ATCC14672 is the producer of phosphoglycolipid antibiotics moenomycins that for almost 40 years were used worldwide as an animal feed additive. As the use of moenomycins narrows down (due to bans in the EU and some other countries), it opens the opportunity to develop much-needed antibiotics against Gram-positive human pathogens, such as cocci. It is desirable to develop ATCC14672 strains accumulating only certain members of moenomycin family which would facilitate their purification, analysis and/or chemical modification. Here we tested site-specific recombinases (SSRs) as a tool to manipulate the genome of ATCC14672 and to achieve aforementioned goals. We show that of three SSRs tested--Cre, Dre, and Flp--the first two efficiently catalyzed recombination reactions, while Flp showed no activity in ATCC14672 cells. Cre recombinase can be reused at least three times to modify ATCC14672 genome without detrimental effects, such as large-scale inversions or deletions. Properties of the generated strains and SSRs are discussed.
journal_name
J Appl Genetjournal_title
Journal of applied geneticsauthors
Lopatniuk M,Ostash B,Makitrynskyy R,Walker S,Luzhetskyy A,Fedorenko Vdoi
10.1007/s13353-015-0283-8subject
Has Abstractpub_date
2015-11-01 00:00:00pages
547-550issue
4eissn
1234-1983issn
2190-3883pii
10.1007/s13353-015-0283-8journal_volume
56pub_type
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