Abstract:
:Disheveled-axin (DIX) domain containing 1 (DIXDC1), a protein containing a coiled-coil domain and a DIX domain, is involved in the progression of multiple cancers. However, the role of DIXDC1 in human pancreatic ductal adenocarcinoma (PDAC) remains unclear. In this study, we investigated the role and prognostic value of DIXDC1 in the development of human PDAC. Western blot analysis revealed that DIXDC1 was highly expressed in PDAC tissues and cell lines. Immunohistochemistry on 165 paraffin-embedded sections showed that high expression of DIXDC1 was significantly correlated with tumor size (P = .002), histological differentiation (P = .001), tumor node metastasis (TNM) stage (P = .001), and the proliferation marker Ki-67 (P = .000). Importantly, Kaplan-Meier analysis revealed that high expression of DIXDC1 was obviously correlated with worsened overall survival (P < .001). In vitro, using serum starvation-refeeding experiments, our results suggested that DIXDC1 was up-regulated in proliferating PDAC cells, together with the percentage of cells at the S phase, and was correlated with the expression of cyclin D1. In addition, depletion of DIXDC1 decreased PCNA and cyclin D1 levels. Accordingly, CCK-8, colony formation, and flow cytometry analyses revealed that knocking down DIXDC1 induced growth impairment and G1/S cell cycle arrest in PDAC cells, while overexpression of DIXDC1 led to accelerated cell proliferation and cell cycle progression. On the basis of these results, we propose that DIXDC1 could play an important role in the tumorigenesis of PDAC and serve as a potential therapeutical target to prevent PDAC progression.
journal_name
Hum Patholjournal_title
Human pathologyauthors
Li X,Xiao Y,Fan S,Xiao M,Wang X,Zhu X,Chen X,Li C,Zong G,Zhou G,Wan Cdoi
10.1016/j.humpath.2016.07.015subject
Has Abstractpub_date
2016-11-01 00:00:00pages
182-192eissn
0046-8177issn
1532-8392pii
S0046-8177(16)30167-8journal_volume
57pub_type
杂志文章相关文献
HUMAN PATHOLOGY文献大全abstract::Primary dermal melanoma (PDM) is a rare variant of melanoma which simulates metastatic melanoma to the skin. Diagnosis of PDM cannot be established on histologic grounds alone but requires absence of evidence of melanoma elsewhere by clinical history and/or imaging studies. Despite this entity being clinically well do...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2020.09.002
更新日期:2020-12-01 00:00:00
abstract::Histologic prognostic parameters in papillary renal cell carcinoma (PRCC) are unclear. The aims were to review the clinicopathological features of PRCC, including Fuhrman grade and International Society of Urological Pathology (ISUP) nucleolar grade, and to identify parameters that may be independent prognostic indica...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2015.07.001
更新日期:2015-10-01 00:00:00
abstract::One hundred six liver biopsy specimens from 34 orthotopic liver transplant (OLT) patients were examined and the histologic findings correlated with the clinical course of the patients to determine if specific morphologic patterns were associated with specific causes of acute allograft dysfunction. The principle causes...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(88)80268-5
更新日期:1988-07-01 00:00:00
abstract::To investigate the roles of matrix metalloproteinases (MMPs) in thymic epithelial tumors, we examined the expression of MMP-2, -7, and -9; membrane-type 1 (MT1)-MMP; and tissue inhibitor of metalloproteinase-2 (TIMP-2) in 57 tumors by immunohistochemistry and in selected 15 cases by in situ hybridization. The tumors c...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2003.07.013
更新日期:2003-12-01 00:00:00
abstract::The aim of the study was to evaluate the impact of the updated 2018 American Society of Clinical Oncology (ASCO)/College of American Pathologists (CAP) guideline on human epidermal growth factor receptor 2 (HER2) interpretation in breast cancer compared with that of the previous guidelines and also the significance of...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2020.01.003
更新日期:2020-04-01 00:00:00
abstract::A 60-year-old man was admitted for progressive visual loss in both eyes. He was diagnosed with retinopathy and chest computed tomography revealed an invasive thymoma. In western blot analysis, serum autoantibodies against recoverin, photoreceptor-specific calcium-binding protein, and heat shock cognate protein 70 whic...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(03)00183-7
更新日期:2003-07-01 00:00:00
abstract::Paraffin-embedded sections of 99 human adrenal and extraadrenal paragangliomas were analyzed by the indirect immunoperoxidase technique for the presence of neuron-specific enolase (NSE) and 10 neuropeptides. Each showed diffuse staining for NSE. Most tumors were positive for [Leu5]-enkephalin (76 per cent), [Met5]-enk...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(88)80314-9
更新日期:1988-01-01 00:00:00
abstract::Human epidermal growth factor receptor 2 (HER2) status is useful for predicting response to trastuzumab. Fluorescence in situ hybridization (FISH) for HER2 gene amplification is accurate but limited because of cost, the need for fluorescence microscopy, the limited assessment of histology, and the fading of its signal...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2013.07.005
更新日期:2013-11-01 00:00:00
abstract::Aberrant crypt foci (ACF) are one of the earliest putative preneoplastic, and in some cases, neoplastic lesions in human colons. These microscopic lesions, identified on methylene blue-stained mucosa with a low-power-magnification microscope, are thought to be closely related to the earliest steps in multistage coloni...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(98)90062-4
更新日期:1998-05-01 00:00:00
abstract::Recently, we reported 2 mixed endometrioid endometrial carcinomas with a "low-grade serous"-like component, which does not fit into any of the 4 molecular groups described by The Cancer Genome Atlas. To understand the nature of these tumors, we have done an immunohistochemical and molecular genetic study of these 2 ca...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2017.10.016
更新日期:2018-01-01 00:00:00
abstract::Neuronal intranuclear inclusion disease (NIID) is a progressive, usually fatal degenerative neurologic condition characterized by eosinophilic, intranuclear inclusions in neurons of the central and peripheral nervous system. We report a boy with onset of disease manifestations at age 3 and death at age 9, who showed c...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/0046-8177(91)90296-2
更新日期:1991-07-01 00:00:00
abstract::To increase awareness of the possible coexistence of an ovarian and breast carcinoma in a BRCA1 mutation carrier, we describe a patient with primary ovarian cancer who developed distant metastases after 3 years and again after 6 years. Surprisingly, at that time an occult breast carcinoma was identified to be the orig...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2003.11.008
更新日期:2004-05-01 00:00:00
abstract::Congenital cystic airway malformation/congenital pulmonary airway malformation (CCAM/CPAM) of the lung is a rare but well-described malformative lesion of pulmonary parenchyma characterized by the abnormal maturation of airways along with an increase in terminal respiratory structures, resulting in cysts of variable s...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2007.04.009
更新日期:2007-12-01 00:00:00
abstract::Serous surface carcinoma (SSC) is a neoplasm histologically indistinguishable from typical serous carcinomas that arise from the ovary but has a distinct clinical presentation. It is characterized by widespread peritoneal dissemination at presentation, but the ovaries are grossly normal in size and shape. If the carci...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(99)90277-0
更新日期:1999-02-01 00:00:00
abstract::Gastric heterotopia of the intestinal tract can have a diverse clinicopathologic presentation, resulting in a diagnostic dilemma. We present a series of four cases, two male and two female patients with age range of 31-82 years, found in the duodenum, jejunum, and transverse colon. The most common and rather unusual c...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2018.01.003
更新日期:2018-04-01 00:00:00
abstract::Four cases of large cell calcifying Sertoli cell tumor, 3 benign and 1 malignant, with no clinical signs of Carney complex or Peutz-Jeghers syndrome are reported with results of histologic, immunohistochemical, ultrastructural, and comparative genomic hybridization studies. Analysis of PRKAR1A gene was performed on 2 ...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2009.09.008
更新日期:2010-04-01 00:00:00
abstract::Patients undergoing treatment of acute lymphoblastic leukemia are at risk for fungal infections including disseminated candidiasis. We describe a case of systemic Candida albicans infection associated with life-threatening gastrointestinal hemorrhage due to unusual necrotizing vasculitis involving the gastrointestinal...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2009.09.015
更新日期:2010-04-01 00:00:00
abstract::GAEC1 is a novel gene located at 7q22.1 that was detected in our previous work in esophageal cancer. The aims of the present study are to identify the copy number of GAEC1 in different colorectal tissues including carcinomas, adenomas, and nonneoplastic tissues and characterize any links to pathologic factors. The cop...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2009.11.014
更新日期:2010-07-01 00:00:00
abstract::DNA methyltransferase 1, 3a, and 3b affect DNA methylation, and it is thought that they play an important role in the malignant transformation of various cancers. The current study was designed to analyze DNA methyltransferase expression by immunohistochemistry in a series of 94 Tunisian sporadic breast carcinomas. Re...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2011.12.022
更新日期:2012-10-01 00:00:00
abstract::Topoisomerase IIalpha (topoIIalpha) is an enzyme required for DNA replication and a molecular target for drugs called anthracyclines. The topoIIalpha gene (TOP2A) is located close to the HER-2/neu oncogene (HER2). We assessed gastric cancers to (1) clarify the relationship between gene amplification and protein overex...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2006.05.008
更新日期:2006-10-01 00:00:00
abstract::We studied the histopathologic, immunohistochemical, and ultrastructural features of the extracellular matrix (ECM) in 20 immunologically defined large-cell lymphomas, including immunoblastic sarcoma of B cells (three patients), peripheral T cell lymphoma (five patients), large non-cleaved follicular center cell (FCC)...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/0046-8177(89)90181-0
更新日期:1989-02-01 00:00:00
abstract::Clear cell papillary renal cell carcinoma (CCPRCC) is a low-grade renal neoplasm with morphological characteristics mimicking both clear cell renal cell carcinoma (CCRCC) and papillary renal cell carcinoma (PRCC). However, despite some overlapping features, their morphological, immunohistochemical, and molecular profi...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2014.01.013
更新日期:2014-06-01 00:00:00
abstract::Recent studies demonstrated that protease-activated receptor-2 (PAR-2) correlates with tumor progression in various tissues. On the other hand, oxidative stress arising from endometriosis has been considered a cause of carcinogenesis in ovarian clear cell carcinoma (OCCC). We previously demonstrated that oxidative str...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2017.04.008
更新日期:2017-06-01 00:00:00
abstract::Multifocal/multicentric breast cancers are common. However, investigations of biomarkers such as estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2 in individual tumor foci of such cancers are rare. This study was designed to evaluate the status of the hormone receptors, human epide...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2010.08.026
更新日期:2012-01-01 00:00:00
abstract::Graduate training is one of the most important activities in which we engage. Through PhD training programs in pathology, we continually renew the intellectual vigor of our discipline and assure its position as a vibrant force in the life sciences. To focus on this important issue, the Education Committee of the Ameri...
journal_title:Human pathology
pub_type: 杂志文章,评审
doi:10.1016/j.humpath.2004.03.014
更新日期:2004-07-01 00:00:00
abstract::Basaloid carcinoma (BC) and large-cell neuroendocrine carcinoma (LCNEC) are 2 recently recognized variants of large-cell lung carcinomas that may overlap in their morphology, and are discriminated by expression of neuroendocrine markers in LCNEC. Because thyroid transcription factor 1 (TTF-1) is expressed in lung aden...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1053/hupa.2001.27110
更新日期:2001-09-01 00:00:00
abstract::Massive chronic intervillositis (MCI) is an unusual placental lesion associated with poor fetal growth and adverse pregnancy outcome; it has not previously been associated with spontaneous abortion or recurrent pregnancy loss. This article reports a patient who had 10 spontaneous abortions with repetitious massive chr...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/0046-8177(95)90201-5
更新日期:1995-11-01 00:00:00
abstract::Insulin-like growth factor-II messenger RNA-binding protein 3 (IGF2BP3 or IMP3) is a biomarker whose expression has been found to be a negative prognostic factor in several neoplasms including ovarian clear cell carcinoma (CCC). In this study, we analyzed the frequency and clinicopathologic significance of IMP3 expres...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2012.12.003
更新日期:2013-08-01 00:00:00
abstract::Paracoccidioidomycosis is a systemic mycosis with a geographic distribution that is limited to Central and South America; Brazil has the highest number of cases. Severe disseminated disease caused by paracoccidioidomycosis was observed in acquired immunodeficiency syndrome patients who live or have resided in endemic ...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2010.03.007
更新日期:2010-09-01 00:00:00
abstract::Cardiac troponin T (cTnT) is considered as a specific marker for acute myocardial infarction. Here, we present a case with elevated cTnT, determined by a third-generation assay, without signs of a myocardial lesion. Routine investigation of a 66-year-old female patient with indolent B-cell lymphoma revealed increased ...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2005.06.009
更新日期:2005-08-01 00:00:00