Abstract：
BACKGROUND:Treatment of Cutaneous Leishmaniasis (CL) is being faced with serious difficulties in Fars Province, due to emerging of resistance against meglumine antimonite (Glucantime®). In this context, determining some biomarkers for drug sensitivity monitoring seems to be highly essential. Different studies have been carried out to decipher the genes might be involved in antimony resistant phenotype in Leishmania spp. Here, we selected three genes: AQP (as drug transporter), TDR-1-1(as drug activator), and γ-GCS (inducing reduction environment) for comparative expression analysis on clinical resistant and sensitive isolates of L. major. METHODS:The clinical isolates of L. major were collected from CL patients referred to Valfajr Health Center, Shiraz from Oct 2011 to Feb 2012. The susceptibility test was performed to confirm drug sensitivity of strains in vitro as well. Then, the gene expression analysis was performed by quantitative real-time PCR using SYBR® Green. RESULTS:By comparison of expression level between strains, up regulation of γ-GCS gene and down regulation of AQP gene were observed in resistant strains compared to the sensitive isolates; however, down regulation of AQP was not statistically specific. Analysis of TDR-1-1 gene unexpectedly showed a high level of expression in the non-responsive cases. CONCLUSION:The γ-GCS, at least, can be considered as a suitable molecular marker for screening antimony sensitivity in clinical isolates, although AQP and TDR-1-1gene seem not to be reliable resistant markers.
journal_name
Iran J Parasitoljournal_title
Iranian journal of parasitologyauthors
Ghobakhloo N,Motazedian MH,Fardaei Msubject
Has Abstractpub_date
2016-04-01 00:00:00pages
168-176issue
2eissn
1735-7020issn
2008-238Xjournal_volume
11pub_type
杂志文章abstract：BACKGROUND:Echinococcosis is a zoonotic parasitic disease of humans and various herbivorous domestic animals transmitted by the contact with domestic and wild carnivores, mainly dogs and foxes. The aim of this study is the production, purification and evaluation immunogenicity of new construction of EG95 protein. METH...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2014-10-01 00:00:00
abstract：BACKGROUND:Coccidiosis of domestic fowl, caused by species of the Genus Eimeria, is responsible for important economic losses in poultry production. Because different species and/or strains can vary in pathogenicity and other biological parameters, their precise characterization is important for epizootiological studie...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2011-12-01 00:00:00
abstract：BACKGROUND:Leishmania Homologue of receptors for Activated C Kinase (LACK) antigen is a 36-kDa protein, which provokes a very early immune response against Leishmania infection. There are several reports on the expression of LACK through different life-cycle stages of genus Leishmania, but only a few of them have focus...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2014-10-01 00:00:00
abstract：BACKGROUND:Due to the lack of a suitable and economic test for the analysis of the polymorphism at codon 167, we developed a new PCR-RFLP technique, based on a modified forward primer (UT-HC167 MF-primer), to identify simultaneously the SNPs at codons 167 and 200 of isotype 1 β-tubulin gene of Haemonchus contortus. ME...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：BACKGROUND:We evaluated the in vivo activity of Bunium persicum (Boiss) essential oil on infected mice with acute toxoplasmosis. METHODS:To evaluate prophylactic effects, male NMRI mice received B. persicum essential oil at the concentrations of 0.05 and 0.1 mL/kg for 14 days. After 24 h mice were infected intraperito...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2015-10-01 00:00:00
abstract：Background:Although rigorous efforts have substantially decreased the malaria burden through decades, it still threatens the lives of millions of children. Development of an effective vaccine can provide important approach in malaria control strategies. Unfortunately, development of an effective vaccine for falciparum ...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章,评审
doi：
更新日期：2018-01-01 00:00:00
abstract：:In the urine of a patient with chronic prostatitis, renal microlithiasis and acute cystitis we found the ciliate protozoa Colpoda spp., both in vegetative and cystic form. The entry point was most likely the urinary tract. Keeping in mind that only four more cases of Colpoda spp. existent in human urine have already b...
journal_title：Iranian journal of parasitology
pub_type：
doi：
更新日期：2011-12-01 00:00:00
abstract：:Hydatid cyst caused by Echinococcus granulosus usually involves lung and liver but can appear in other organs. We report a 29-yr-old woman presented to Fatemi Hospital, Ardabil, Iran in 2017 with progressive painful swelling of the left gluteus which in imaging showed hydatid cyst. The cyst was successfully en blocked...
journal_title：Iranian journal of parasitology
pub_type：
doi：
更新日期：2019-07-01 00:00:00
abstract：BACKGROUND:The number of malaria cases is declining worldwide; however, it remains as a serious health problem. Diagnosing unusual cases is the most important issue to manage the problem. This study designed to describe the number of falciparum and vivax malaria infected patients referred to Malaria Reference Laborator...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2015-10-01 00:00:00
abstract：BACKGROUND:Schistosomiasis is a debilitating disease, infects millions of people in tropical and subtropical regions. This study assessed the situation of the urinary schistosomiasis among schoolchildren of the Alsaial Alsagair village, Sudan. METHODS:A cross-sectional survey was carried out in 2016, in the Alsaial Al...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2017-04-01 00:00:00
abstract：Background:Cystic echinococcosis can cause severe disease and probable death in humans. Epitopes of its antigens play a key role in the sensitivity and specificity of immunodiagnostic tests. Methods:Epitope prediction software programs predict the most antigenic linear B-cell epitopes of AgB (8 kD), Ag5, and Ag95. Six...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2020-01-01 00:00:00
abstract：Background:This study aimed to set-up latex agglutination test (LAT) and ELISA based on recombinant A2 from Iranian strain of Leishmania (L.) infantum (rA2-Ag) and evaluated for detection of anti-Leishmania antibodies in dogs compared to standard direct agglutination test (DAT). Methods:The rA2-Ag was synthesized unde...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2018-04-01 00:00:00
abstract：BACKGROUND:Leishmania tropica is a genetically divergent species. Amplification of entire internal transcribed spacer (ITS) region of L. tropica isolates obtained from Bam district, one of the well known focus of anthroponotic cutaneous leishmaniasis (ACL) in Iran, revealed a double-band pattern in agarose gel electrop...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-04-01 00:00:00
abstract：Background:The current study aimed to investigate the possible cross-protective effects of attenuated L. major against L. infantum in BALB/c mice. Methods:This experimental study was performed in 2017 in Shiraz University of Medical Sciences, Shiraz, Iran. The attenuated strain of L. major was prepared by continuous w...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2019-04-01 00:00:00
abstract：:Herein, a 28-year-old man with hoarseness, skin and oral lesions is presented. At the time of admission, the patient had an erythematous plaque on his chin near his lower lip and an erythematous-violaceous plaque on his palate near the opening of the pharynx and 20 kg weight lost in last one year. The biopsy of his sk...
journal_title：Iranian journal of parasitology
pub_type：
doi：
更新日期：2015-04-01 00:00:00
abstract：Background:Intestinal parasites are one of the health challenges in developing countries. Decreasing the prevalence of intestinal parasitic infections (IPIs) is one of the main aims of health services in these countries. This study was designed to determine the current status of IPIs in rural residents of Takestan a to...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2019-10-01 00:00:00
abstract：Background:To assay the Trichinella-specific IgM and IgG antibody responses during the early stage of infection, serum was collected from mice infected with the muscle larvae (ML) of T. spiralis (ISS534) at different dpi (days post infection) up to 60 days. Methods:The levels of IgM and IgG antibodies in serum were me...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2019-04-01 00:00:00
abstract：BACKGROUND:The objective of this study was to investigate HLA-DRB1*and DQB1* allelic polymorphisms in Iranian patients with hydatidose. This is the first survey dealing with the correlation between HLA-DRB1* and DQB1* alleles and cystic echinococcosis in Iranian patients. METHODS:The study was carried out on 56 patien...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-10-01 00:00:00
abstract：BACKGROUND:Coccidiosis is a serious protozoal disease of poultry. The identification of Eimeria species has important implications for diagnosis and control as well as for epidemiology. The molecular characterization of Eimeria species infecting Egyptian baladi chickens was investigated. METHODS:Eimeria species oocyst...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
abstract：BACKGROUND:Leishmaniasis is a sand fly-borne disease caused by the protozoan parasites belonging to the genus Leishmania. Because of the preventing and controlling methods, clinical course, prognosis and choice of treatment are differing from species; differentiation of species is critical. The present study was aimed ...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2016-10-01 00:00:00
abstract：Background:The effectiveness of anthelmintics may diminish within approximately 10 yr of use relying on several factors such as anthelmintic resistance. This study aimed to assess in vitro effect of Balanites aegyptiaca fruits extract on the cuticle of adult worm of Toxocara canis as naturally alternative therapy. Met...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2018-10-01 00:00:00
abstract：BACKGROUND:Leishmaniasis is a group of diseases that are created by intracellular parasites of Leishmania. Cutaneous leishmaniasis is considered as one of the health problems in some provinces of Iran. METHODS:In this study, a total of 178 Giemsa-stained slides from confirmed cases of cutaneous leishmaniasis were exam...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-07-01 00:00:00
abstract：BACKGROUND:Parasitological methods for the diagnosis of Visceral leishmaniasis (VL) require invasive procedures, so serological and molecular approaches have been developed but are not generally applicable in the field. We evaluated a loop mediated isothermal amplification (LAMP) assay using blood from VL patients and ...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2014-03-01 00:00:00
abstract：BACKGROUND:Successful therapy of leishmaniasis depends on effective cellular immune response. We evaluated the effectiveness of sodium selenite and zinc sulphate as known immunomodulator materials, in combination with Glucantime® in treatment of cutaneous leishmaniasis lesions resulting from Leishmania major in suscept...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2010-09-01 00:00:00
abstract：:Contamination of human and animal body tissues with flies' larvae and diptera cause myiasis. A 26 yr old female patient refers to Kashan Shahid Beheshti Hospital, central Iran because of urogenital infection, pain in the right part of stomach, smelly and reddish vaginal discharge and frequent urination. In the first c...
journal_title：Iranian journal of parasitology
pub_type：
doi：
更新日期：2016-07-01 00:00:00
abstract：BACKGROUND:Acanthamoeba is an opportunistic amphizoic protozoan found in different water sources including swimming pool as well as in sewage. The aim of this study was to investigate the prevalence of Acanthamoeba in tap-water samples in Iran. METHOD:In this descriptive cross-sectional study, 94 samples of cold and w...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2010-06-01 00:00:00
abstract：BACKGROUND:The sole published data on feline heartworm infection in the Philippines was reported four decades ago. The study therefore endeavoured to assess and provide an update on the current status of heartworm infection in domesticated feline species using serologic and parasitological examination methods. METHODS...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：BACKGROUND:As for human trichomoniasis the host-parasite relationship is very complex, and the broad ranges of clinical symptoms are unlikely be attributable to a single pathogenic mechanism. Specific Random Amplified Polymorphic DNA (RAPD) markers of 490 bp, 720 bp and 460 bp using the primers Tv-5, OPA-6 and OPA-11, ...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2015-07-01 00:00:00
abstract：BACKGROUND:The objective of the present research was to determine the frequency of Toxocara spp. eggs in soil samples of public parks, in the city of Tehran, Iran. METHODS:A total of 600 soil samples were taken from 120 parks between Aprils to November, 2008. Soil samples were collected from 5 distinct sites in the pa...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：:Visceral leishmaniasis is a common parasitic disease between humans and animals, transmitted by sandflies (Phlebotomus) in the Mediterranean countries, including Iran. The statistics have been reported less than real due to errors in the diagnosis and reporting of affected cases. In this report, we will present the sy...
journal_title：Iranian journal of parasitology
pub_type：
doi：
更新日期：2020-04-01 00:00:00
