Abstract：
BACKGROUND:Coccidiosis is a serious protozoal disease of poultry. The identification of Eimeria species has important implications for diagnosis and control as well as for epidemiology. The molecular characterization of Eimeria species infecting Egyptian baladi chickens was investigated. METHODS:Eimeria species oocysts were harvested from intestines of naturally infected Egyptian baldi chickens. The morphometry characterization of oocysts along with COCCIMORPH software was done. The DNA was extracted initially by freezing and thawing then the prepared samples was subjected to commercial DNA kits. The DNA products were analyzed through conventional polymerase chain reaction by using amplified region (SCAR) marker. RESULTS:The PCR results confirmed the presence of 7 Eimeria species in the examined fecal samples of Egyptian baldi breed with their specific ampilicon sizes being E. acervulina (811bp), E. brunette (626bp), E. tenella (539bp), E. maxima (272bp), E. necatrix (200bp), E. mitis (327bp) and E. praecopx (354bp). A sequencing of the two most predominant species of Eimeria was done, on E. tenella and E. máxima. Analysis of the obtained sequences revealed high identities 99% between Egyptian isolates and the reference one. Similarly, E. maxima isolated from Egyptian baldi chickens showed 98% nucleotide identities with the reference strain. Only single nucleotide substitution was observed among the Egyptian E. tenella isolates (A181G) when compared to the reference one. The Egyptian isolates acquired 4 unique mutations (A68T, C164T, G190A and C227G) in compared with the reference sequence. CONCLUSION:This is the first time to identify the 7 species of Eimeria from Egyptian baladi chickens.
journal_name
Iran J Parasitoljournal_title
Iranian journal of parasitologyauthors
Gadelhaq SM,Arafa WM,Aboelhadid SMsubject
Has Abstractpub_date
2015-01-01 00:00:00pages
87-95issue
1eissn
1735-7020issn
2008-238Xjournal_volume
10pub_type
杂志文章abstract：BACKGROUND:Leishmaniasis is a group of diseases that are created by intracellular parasites of Leishmania. Cutaneous leishmaniasis is considered as one of the health problems in some provinces of Iran. METHODS:In this study, a total of 178 Giemsa-stained slides from confirmed cases of cutaneous leishmaniasis were exam...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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更新日期：2013-07-01 00:00:00
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journal_title：Iranian journal of parasitology
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更新日期：2015-04-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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更新日期：2018-07-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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更新日期：2014-03-01 00:00:00
abstract：Background:Opisthorchis viverrini is a serious health problem in Southeast Asia. The infection is associated with cholangiocarcinoma. Therefore, this study was aimed to detect O. viverrini infections among the ASEAN population in Thailand. Methods:A cross-sectional study was conducted among 249 individuals from ASEAN ...
journal_title：Iranian journal of parasitology
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更新日期：2018-04-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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更新日期：2010-06-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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更新日期：2014-03-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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更新日期：2015-10-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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更新日期：2018-10-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2014-10-01 00:00:00
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journal_title：Iranian journal of parasitology
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更新日期：2017-10-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2019-04-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2020-01-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2017-01-01 00:00:00
abstract：BACKGROUND:Trichomoniasis is a worldwide protozoan parasitic disease and metronidazole is a choice drug for its treatment. Because of disease importance in public health and its controversial ideas about the prevalence of drug resistance, this study was carried out. METHODS:Fifty-two suspected vaginal samples were col...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2010-12-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2017-04-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2015-07-01 00:00:00
abstract：:Visceral leishmaniasis is a common parasitic disease between humans and animals, transmitted by sandflies (Phlebotomus) in the Mediterranean countries, including Iran. The statistics have been reported less than real due to errors in the diagnosis and reporting of affected cases. In this report, we will present the sy...
journal_title：Iranian journal of parasitology
pub_type：
doi：
更新日期：2020-04-01 00:00:00
abstract：BACKGROUND:The leishmanicidal and cytotoxic activity of ten essential oils obtained from ten plant specimens were evaluated. METHODS:Essential oils were obtained by the steam distillation of plant leaves without any prior processing. Cytotoxicity was tested on J774 macrophages and leishmanicidal activity was assessed ...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2018-01-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type：
doi：
更新日期：2019-07-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2018-04-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type：
doi：
更新日期：2012-01-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2010-03-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-04-01 00:00:00
abstract：:In the urine of a patient with chronic prostatitis, renal microlithiasis and acute cystitis we found the ciliate protozoa Colpoda spp., both in vegetative and cystic form. The entry point was most likely the urinary tract. Keeping in mind that only four more cases of Colpoda spp. existent in human urine have already b...
journal_title：Iranian journal of parasitology
pub_type：
doi：
更新日期：2011-12-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章,评审
doi：
更新日期：2017-07-01 00:00:00
abstract：BACKGROUND:Leishmaniasis is a sand fly-borne disease caused by the protozoan parasites belonging to the genus Leishmania. Because of the preventing and controlling methods, clinical course, prognosis and choice of treatment are differing from species; differentiation of species is critical. The present study was aimed ...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2016-10-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章,评审
doi：
更新日期：2018-01-01 00:00:00
