Abstract：
BACKGROUND:Echinococcosis is a zoonotic parasitic disease of humans and various herbivorous domestic animals transmitted by the contact with domestic and wild carnivores, mainly dogs and foxes. The aim of this study is the production, purification and evaluation immunogenicity of new construction of EG95 protein. METHODS:The recombinant plasmid pET32-a+ used for Eg95 expression was constructed with the EG95 gene of Echinococcus granulosus fused with the thioredoxin tag. This recombinant clone was over expressed in Escherichia coli BL-21 (DE-3). The expressed fusion protein was found almost entirely in the insoluble form (inclusion bodies) in cell lysate. The purification was performed under denaturing conditions in the presence of 8M urea by Ni-NTA column and dialysis. The purified recombinant proteins were confirmed with western blot analysis using polyclonal antiserum. To find out the immunogenicity of the purified protein, the BALB/c mice (10 mice/group) were immunized by injecting 20 μg rEG95 protein formulated in Freund's and alum adjuvant. RESULTS:Immunization of mice with rEG95 using CFA/IFA and alum adjuvant generated high level of total antibody. In proliferation assay, the lymphocytes were able to mount a strong proliferative response with related production of IFN-γ, IL-12 and TNF-α but with low secretion of either IL-4 or IL-10. The humoral and cellular immune responses against rEG95 suggested a mixed Th1/Th2 response with high intensity toward Th1. CONCLUSION:Our findings suggest that new construct of rEG95 formulated with CFA/IFA and alum adjuvant elicited strong cellular and humoral responses supporting further development of this vaccine candidate.
journal_name
Iran J Parasitoljournal_title
Iranian journal of parasitologyauthors
Pirestani M,Dalimi A,Sarvi S,Khoramabadi Nsubject
Has Abstractpub_date
2014-10-01 00:00:00pages
491-502issue
4eissn
1735-7020issn
2008-238Xjournal_volume
9pub_type
杂志文章abstract：BACKGROUND:Fasciolosis in livestock is a crucial concern in the globe, mainly due to its impact on human health. The aim of this study was to determine the rate of infection with Fasciola gigantica (Cobbold, 1855) larvae in the field-collected snails of Lymnaea auricularia (Linnaeus, 1785) from northwestern Iran using ...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2014-09-01 00:00:00
abstract：BACKGROUND:Treatment of Cutaneous Leishmaniasis (CL) is being faced with serious difficulties in Fars Province, due to emerging of resistance against meglumine antimonite (Glucantime®). In this context, determining some biomarkers for drug sensitivity monitoring seems to be highly essential. Different studies have been...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2016-04-01 00:00:00
abstract：BACKGROUND:This study was conducted to collect informative data on the parasitic infection of wild rodents, emphasizing on finding parasites, which have medical importance to human. METHODS:During 2012-2014, a total number of 91 wild rodents were captured from rural areas of Turkmen Sahra, Golestan Province, using han...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2016-07-01 00:00:00
abstract：BACKGROUND:The objective of the present research was to determine the frequency of Toxocara spp. eggs in soil samples of public parks, in the city of Tehran, Iran. METHODS:A total of 600 soil samples were taken from 120 parks between Aprils to November, 2008. Soil samples were collected from 5 distinct sites in the pa...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：BACKGROUND:Canine visceral leishmaniasis (CVL) is not only an emerging veterinary concern but also a public health threat in endemic areas. The aim of this study was to assess the efficacy, immunogenicity and safety of two doses of aluminum hydroxide (alum) precipitated Leishmania major (Alum-ALM) mixed with BCG plus i...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2015-07-01 00:00:00
abstract：BACKGROUND:We tried to investigate the hair contamination of pet dogs and farm sheepdog with Toxocara eggs in terms of the different sex and age groups in north-west of Iran (Urmia and its suburbs). METHODS:Hair samples were collected from a total of 138 pet and farm sheepdogs from November 2008 to June 2009 in Urmia ...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：:Contamination of human and animal body tissues with flies' larvae and diptera cause myiasis. A 26 yr old female patient refers to Kashan Shahid Beheshti Hospital, central Iran because of urogenital infection, pain in the right part of stomach, smelly and reddish vaginal discharge and frequent urination. In the first c...
journal_title：Iranian journal of parasitology
pub_type：
doi：
更新日期：2016-07-01 00:00:00
abstract：BACKGROUND:The incidence of cutaneous leishmaniasis in the city of Mehran has risen sharply in recent years because the city borders Iraq, which has allowed entrance of different Leishmania strains. These strains have different shapes, periods of disease, and healing of lesions. The present study identified and determi...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2016-01-01 00:00:00
abstract：Background:The effectiveness of anthelmintics may diminish within approximately 10 yr of use relying on several factors such as anthelmintic resistance. This study aimed to assess in vitro effect of Balanites aegyptiaca fruits extract on the cuticle of adult worm of Toxocara canis as naturally alternative therapy. Met...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2018-10-01 00:00:00
abstract：BACKGROUND:Leishmania tropica is a genetically divergent species. Amplification of entire internal transcribed spacer (ITS) region of L. tropica isolates obtained from Bam district, one of the well known focus of anthroponotic cutaneous leishmaniasis (ACL) in Iran, revealed a double-band pattern in agarose gel electrop...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-04-01 00:00:00
abstract：Background:Although rigorous efforts have substantially decreased the malaria burden through decades, it still threatens the lives of millions of children. Development of an effective vaccine can provide important approach in malaria control strategies. Unfortunately, development of an effective vaccine for falciparum ...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章,评审
doi：
更新日期：2018-01-01 00:00:00
abstract：BACKGROUND:Leishmaniasis is a sand fly-borne disease caused by the protozoan parasites belonging to the genus Leishmania. Because of the preventing and controlling methods, clinical course, prognosis and choice of treatment are differing from species; differentiation of species is critical. The present study was aimed ...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2016-10-01 00:00:00
abstract：BACKGROUND:Schistosomiasis is a debilitating disease, infects millions of people in tropical and subtropical regions. This study assessed the situation of the urinary schistosomiasis among schoolchildren of the Alsaial Alsagair village, Sudan. METHODS:A cross-sectional survey was carried out in 2016, in the Alsaial Al...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2017-04-01 00:00:00
abstract：Background:Soil-transmitted helminth infections constitute a public health problem in the rural areas of tropical and subtropical regions, including Thailand. We aimed to determine the prevalence of soil-transmitted helminth infections and underlying risk factors among the schoolchildren living in the rural areas of so...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：10.18502/ijpa.v15i3.4210
更新日期：2020-07-01 00:00:00
abstract：BACKGROUND:Chemotherapy still relies on the use of pentavalent antimonials, amphotericin B, paromomycin, miltefosin, and allopurinol. In this study, we explained about the native plant that grows in different regions of Iran and used as anti-leishmanial in Iran and even many other countries. METHODS:This narrative rev...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章,评审
doi：
更新日期：2017-07-01 00:00:00
abstract：Background:The larval stage of the tapeworm (cestode) Echinococcus granulosus is the etiological agent of hydatidosis or cystic echinococcosis, which is the zoonotic parasitic disease causing morbidity and mortality in both humans and livestock. Due to a lack of accurate data on the human isolates of E. granulosus in M...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2019-01-01 00:00:00
abstract：:Visceral leishmaniasis is a common parasitic disease between humans and animals, transmitted by sandflies (Phlebotomus) in the Mediterranean countries, including Iran. The statistics have been reported less than real due to errors in the diagnosis and reporting of affected cases. In this report, we will present the sy...
journal_title：Iranian journal of parasitology
pub_type：
doi：
更新日期：2020-04-01 00:00:00
abstract：:The presence of Visceral Larva Migrans (VLM) in a patient is reported. A 57-year- old woman suffering from right upper abdominal and suprapubic pain referred into a clinic in Khorramabad, Lorestan Province, Iran. A cystoscopy was performed and biopsy was taken. The light microscopic study showed a couple of larvae as ...
journal_title：Iranian journal of parasitology
pub_type：
doi：
更新日期：2011-03-01 00:00:00
abstract：Background:Opisthorchis viverrini is a serious health problem in Southeast Asia. The infection is associated with cholangiocarcinoma. Therefore, this study was aimed to detect O. viverrini infections among the ASEAN population in Thailand. Methods:A cross-sectional study was conducted among 249 individuals from ASEAN ...
journal_title：Iranian journal of parasitology
pub_type：
doi：
更新日期：2018-04-01 00:00:00
abstract：:Hydatid cyst caused by Echinococcus granulosus usually involves lung and liver but can appear in other organs. We report a 29-yr-old woman presented to Fatemi Hospital, Ardabil, Iran in 2017 with progressive painful swelling of the left gluteus which in imaging showed hydatid cyst. The cyst was successfully en blocked...
journal_title：Iranian journal of parasitology
pub_type：
doi：
更新日期：2019-07-01 00:00:00
abstract：Background:The protozoan parasite Theileria annulata is the causative agent of tropical theileriosis in cattle. Vaccination is recommended by administration of attenuated schizont-infected cell lines. The expected protective immunity post-vaccination can be demonstrated by challenge test through inoculation of highly v...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2019-10-01 00:00:00
abstract：BACKGROUND:Visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL) are important public health problems in Iran. We aimed to evaluate the diagnostic potential of Western blot (WB) compared with indirect immunofluorescence test (IFAT) to serodiagnosis of leishmaniasis. METHODS:This study was performed from 2010-20...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2017-04-01 00:00:00
abstract：:Tenia solium, a parasite causes cysticercous cellulose when affecting the central nervous system, the manifestation is called neurocysticercosis. The most common symptom in neurocysticercosis is seizure. Generally, oral diagnosticians come across cases of oral cysticercosis and it is rare to find a case of neurocystic...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-10-01 00:00:00
abstract：BACKGROUND:The aim of this study was to detect and characterize Cryptosporidium spp. in water samples collected from recreational ponds of Chaharmahal va Bakhtiyari Province of Iran. METHODS:Thirty water samples were collected from November 2009 to May 2010. Each sample contained 10 liters of water. We used the SSU rR...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2011-03-01 00:00:00
abstract：BACKGROUND:Coccidiosis of domestic fowl, caused by species of the Genus Eimeria, is responsible for important economic losses in poultry production. Because different species and/or strains can vary in pathogenicity and other biological parameters, their precise characterization is important for epizootiological studie...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2011-12-01 00:00:00
abstract：BACKGROUND:Cutaneous Leishmaniasis (CL) is a parasitic skin disease. Diagnosis primarily is based on clinical signs and microscopic observation of parasite on direct stained smears or tissue sections. Sensitivity of direct smear is not as high as molecular methods. The aim of this study was to identify and characterize...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-04-01 00:00:00
abstract：Background:Cystic echinococcosis can cause severe disease and probable death in humans. Epitopes of its antigens play a key role in the sensitivity and specificity of immunodiagnostic tests. Methods:Epitope prediction software programs predict the most antigenic linear B-cell epitopes of AgB (8 kD), Ag5, and Ag95. Six...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2020-01-01 00:00:00
abstract：Background:Intestinal parasitic infections (IPIs) are among the most important etiologies of gastrointestinal disorders in developing countries. The present study was performed to determine the prevalence of IPIs in rural inhabitants of Fouman, northern Iran. Methods:Overall, 31 villages were randomly selected during ...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2020-01-01 00:00:00
abstract：BACKGROUND:Cryptosporidiosis is one of the most important parasitic infections in human and animals. This study was designed for survey on the prevalence of Cryptosporidium infection in farms of Ilam, west of Iran, using parasitology method and genotyping by Nested PCR-RFLP. METHODS:Fecal samples of 217 cattle were co...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2014-09-01 00:00:00
abstract：BACKGROUND:Hydatidosis, as the most important zoonotic parasitic disease in Iran, has posed many health and economic losses. This study was conducted to investigate the demographic characteristics of hydatid cyst surgeries in hospitals of East Azerbaijan Province, Northwest of Iran. METHODS:Demographic characteristics...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2014-04-01 00:00:00
