Abstract:
Background:The protozoan parasite Theileria annulata is the causative agent of tropical theileriosis in cattle. Vaccination is recommended by administration of attenuated schizont-infected cell lines. The expected protective immunity post-vaccination can be demonstrated by challenge test through inoculation of highly virulent infective sporozoites. The aim of this study was to produce Hyalomma anatolicum anatolicum tick infected with T. annulata (local strain) for preparation of tick-derived sporozoite stabilates for molecular characterization and infectivity test assay. Methods:A local T. annulata strain was used for experimental infection of calves. A field isolate of H. a. anatolicum was isolated, laboratory-reared and infected by blood-feeding on Theileria infected above-mentioned calves. The infectivity of calf, tick and prepared stabilate were confirmed by clinical signs of theileriosis, microscopic inspection, RT-PCR and in vitro cell culture. Results:The tick stabilate was prepared and cryopreserved in liquid nitrogen. The infectivity of the tick stabilate was verified by in vivo bioassay, in vitro cell culture infection, microscopic inspection in salivary glands and RT-PCR assay. The in vitro produced cell line in this study was characterized by T. annulata Cytochrome b gene analyzing. Conclusion:The infectivity of a new prepared tick-derived sporozoite stabilate was confirmed in susceptible calves; by microscopically, post mortem, tick microscopic and molecular assays. Moreover, naïve PBMCs were transformed and proliferated by T. annulata infected tick stabilate to immortal T. annulata schizont infected cell line. The potent infective sporozoite tick derived stabilate could be used for vaccine efficacy and challenge test as well as in vaccine development.
journal_name
Iran J Parasitoljournal_title
Iranian journal of parasitologyauthors
Modirrousta H,Habibi G,Shayan P,Afshari A,Mirjalili A,Abdigoudarzi Msubject
Has Abstractpub_date
2019-10-01 00:00:00pages
572-583issue
4eissn
1735-7020issn
2008-238Xjournal_volume
14pub_type
杂志文章abstract:Background:Although rigorous efforts have substantially decreased the malaria burden through decades, it still threatens the lives of millions of children. Development of an effective vaccine can provide important approach in malaria control strategies. Unfortunately, development of an effective vaccine for falciparum ...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章,评审
doi:
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Cutaneous Leishmaniasis (CL) is a parasitic skin disease. Diagnosis primarily is based on clinical signs and microscopic observation of parasite on direct stained smears or tissue sections. Sensitivity of direct smear is not as high as molecular methods. The aim of this study was to identify and characterize...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2013-04-01 00:00:00
abstract::We report a case of human nasal nosocomial myiasis in a 69-year-old rural man with numerous live maggots in nostrils. The patient was admitted in Emergency Ward due to dyspnea. After 72 hours the companion of patient discovered larva. The presence of the third instar larva indicated that the infestation was not more t...
journal_title:Iranian journal of parasitology
pub_type:
doi:
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:Clinical manifestations of Strongyloides stercoralis are variable from asymptomatic to hyperinfection and devastating disseminated infections. Hereby, clinical characteristics of a large series of Iranian strongyloidiasis indigenous cases are described. METHODS:The records of people referred to the Helminth...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2014-04-01 00:00:00
abstract:BACKGROUND:As for human trichomoniasis the host-parasite relationship is very complex, and the broad ranges of clinical symptoms are unlikely be attributable to a single pathogenic mechanism. Specific Random Amplified Polymorphic DNA (RAPD) markers of 490 bp, 720 bp and 460 bp using the primers Tv-5, OPA-6 and OPA-11, ...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2015-07-01 00:00:00
abstract:Background:The effectiveness of anthelmintics may diminish within approximately 10 yr of use relying on several factors such as anthelmintic resistance. This study aimed to assess in vitro effect of Balanites aegyptiaca fruits extract on the cuticle of adult worm of Toxocara canis as naturally alternative therapy. Met...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2018-10-01 00:00:00
abstract::The presence of Visceral Larva Migrans (VLM) in a patient is reported. A 57-year- old woman suffering from right upper abdominal and suprapubic pain referred into a clinic in Khorramabad, Lorestan Province, Iran. A cystoscopy was performed and biopsy was taken. The light microscopic study showed a couple of larvae as ...
journal_title:Iranian journal of parasitology
pub_type:
doi:
更新日期:2011-03-01 00:00:00
abstract:Background:Intestinal parasitic infections (IPIs) are among the most important etiologies of gastrointestinal disorders in developing countries. The present study was performed to determine the prevalence of IPIs in rural inhabitants of Fouman, northern Iran. Methods:Overall, 31 villages were randomly selected during ...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:Visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL) are important public health problems in Iran. We aimed to evaluate the diagnostic potential of Western blot (WB) compared with indirect immunofluorescence test (IFAT) to serodiagnosis of leishmaniasis. METHODS:This study was performed from 2010-20...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2017-04-01 00:00:00
abstract:Background:We aimed at genotyping and evaluating the predominance of G. duodenalis assemblages isolated from patients referred to medical laboratories in Khorramabad, Iran from Nov 2015 to Sep 2016. Hence, the development of a cost-effective HRM approach to determine genotypes of G. duodenalis based on the triosephosph...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2020-04-01 00:00:00
abstract:Background:To assay the Trichinella-specific IgM and IgG antibody responses during the early stage of infection, serum was collected from mice infected with the muscle larvae (ML) of T. spiralis (ISS534) at different dpi (days post infection) up to 60 days. Methods:The levels of IgM and IgG antibodies in serum were me...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2019-04-01 00:00:00
abstract:BACKGROUND:Leishmaniasis is a parasitic infection that may lead to a variety of manifestations. In Iran, cutaneous leishmaniasis (CL) has a high prevalence. There are many treatment modalities for CL. The use of oral terbinafine in the treatment of CL has recently been considered. The aim of this study was to compare c...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:The aim of this study was to detect and characterize Cryptosporidium spp. in water samples collected from recreational ponds of Chaharmahal va Bakhtiyari Province of Iran. METHODS:Thirty water samples were collected from November 2009 to May 2010. Each sample contained 10 liters of water. We used the SSU rR...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2011-03-01 00:00:00
abstract:BACKGROUND:Due to the lack of a suitable and economic test for the analysis of the polymorphism at codon 167, we developed a new PCR-RFLP technique, based on a modified forward primer (UT-HC167 MF-primer), to identify simultaneously the SNPs at codons 167 and 200 of isotype 1 β-tubulin gene of Haemonchus contortus. ME...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2012-01-01 00:00:00
abstract:Background:Cystic echinococcosis can cause severe disease and probable death in humans. Epitopes of its antigens play a key role in the sensitivity and specificity of immunodiagnostic tests. Methods:Epitope prediction software programs predict the most antigenic linear B-cell epitopes of AgB (8 kD), Ag5, and Ag95. Six...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2020-01-01 00:00:00
abstract:Background:Due to the limited number of the available drugs for the treatment of trypanosomiasis, this study was designed to evaluate the trypanocidal effects of cisplatin or/and Nigella sativa oil (NSO) in experimentally infected mice with T. evansi. Methods:During 2015 at the Parasitology Department, Menoufia Univer...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2018-01-01 00:00:00
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journal_title:Iranian journal of parasitology
pub_type:
doi:
更新日期:2020-04-01 00:00:00
abstract:BACKGROUND:Plasmodium vivax is the most widespread species of Plasmodium in humans and causing about 80 million clinical cases annually. This study was undertaken to detect P. vivax in asymptomatic treated vivax malaria patients to trace latent/sub-patent malaria infection. METHOD:The venous blood of all detected case...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2013-10-01 00:00:00
abstract:BACKGROUND:We tried to investigate the hair contamination of pet dogs and farm sheepdog with Toxocara eggs in terms of the different sex and age groups in north-west of Iran (Urmia and its suburbs). METHODS:Hair samples were collected from a total of 138 pet and farm sheepdogs from November 2008 to June 2009 in Urmia ...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2012-01-01 00:00:00
abstract:Background:The Totiviridae family includes a number of double-stranded RNA viruses that can infect Trichomonas vaginalis. Some T. vaginalis isolates are infected with one or more double-stranded RNA (dsRNA) viruses. In this study, different strains of double-stranded RNA virus in Iranian isolates of T. vaginalis were e...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:10.18502/ijpa.v15i3.4200
更新日期:2020-07-01 00:00:00
abstract:BACKGROUND:Treatment of Cutaneous Leishmaniasis (CL) is being faced with serious difficulties in Fars Province, due to emerging of resistance against meglumine antimonite (Glucantime®). In this context, determining some biomarkers for drug sensitivity monitoring seems to be highly essential. Different studies have been...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2016-04-01 00:00:00
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journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2016-10-01 00:00:00
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journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2015-10-01 00:00:00
abstract:BACKGROUND:Hydatidosis, as the most important zoonotic parasitic disease in Iran, has posed many health and economic losses. This study was conducted to investigate the demographic characteristics of hydatid cyst surgeries in hospitals of East Azerbaijan Province, Northwest of Iran. METHODS:Demographic characteristics...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2014-04-01 00:00:00
abstract:BACKGROUND:Cryptosporidiosis is one of the most important parasitic infections in human and animals. This study was designed for survey on the prevalence of Cryptosporidium infection in farms of Ilam, west of Iran, using parasitology method and genotyping by Nested PCR-RFLP. METHODS:Fecal samples of 217 cattle were co...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2014-09-01 00:00:00
abstract:BACKGROUND:Schistosomiasis is a debilitating disease, infects millions of people in tropical and subtropical regions. This study assessed the situation of the urinary schistosomiasis among schoolchildren of the Alsaial Alsagair village, Sudan. METHODS:A cross-sectional survey was carried out in 2016, in the Alsaial Al...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2017-04-01 00:00:00
abstract:Background:In Iran, both forms of cutaneous (CL) and visceral leishmaniasis (VL) have been re-ported; so the accurate species identification of the parasite(s) and the analysis of genetic diversity are necessary. Methods:The investigation was conducted from 2014 to 2015 in the northwest and south of Iran, where VL is ...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2018-10-01 00:00:00
abstract:Background:This cross-sectional investigation aimed to evaluate the prevalence of IgM and IgG anti-Toxoplasma gondii antibodies and the associated risk factors among healthy blood donors in Khorasan Razavi Province, northeast of Iran from Nov 2014 to May 2015. Methods:Overall, 491 serum samples from apparently healthy...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2017-10-01 00:00:00
abstract:BACKGROUND:Leishmania Homologue of receptors for Activated C Kinase (LACK) antigen is a 36-kDa protein, which provokes a very early immune response against Leishmania infection. There are several reports on the expression of LACK through different life-cycle stages of genus Leishmania, but only a few of them have focus...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2014-10-01 00:00:00
abstract:Background:The current study aimed to investigate the possible cross-protective effects of attenuated L. major against L. infantum in BALB/c mice. Methods:This experimental study was performed in 2017 in Shiraz University of Medical Sciences, Shiraz, Iran. The attenuated strain of L. major was prepared by continuous w...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2019-04-01 00:00:00