Abstract：
BACKGROUND:The incidence of cutaneous leishmaniasis in the city of Mehran has risen sharply in recent years because the city borders Iraq, which has allowed entrance of different Leishmania strains. These strains have different shapes, periods of disease, and healing of lesions. The present study identified and determined cutaneous leishmaniasis species in this region. METHODS:This cross-sectional study was carried out by preparing slides from 92 patients with suspected cutaneous leishmaniasis lesions from Mehran during 2012-2013. Parasite genomic DNA was extracted and CSB2XF and CSB1XR primers were used to amplify the Leishmania minicircle kDNA regions. The parasite species were detected by specific 13Z and LIR primers by applying nested PCR technique. RESULTS:All banding patterns were diagnosed as L. major parasite by comparison of standard models with amplified fragments 560 bp in length from bands. The patients were 56.5% male and 43.5% female. The most frequently-infected age group was the 21-30 years group at a rate of 27.2%. About 56.3% of patients had a single lesion and a significant correlation was observed between age and number of lesions (P > 0.05). CONCLUSION:The nested PCR technique was shown to be an effective method with high sensitivity and specificity for identification of human Leishmania parasites. Molecular analysis revealed that parasites isolated from Mehran were identified as L. major and the disease was rural in form.
journal_name
Iran J Parasitoljournal_title
Iranian journal of parasitologyauthors
Feiz Haddad MH,Ghasemi E,Maraghi S,Tavala Msubject
Has Abstractpub_date
2016-01-01 00:00:00pages
65-72issue
1eissn
1735-7020issn
2008-238Xjournal_volume
11pub_type
杂志文章abstract：BACKGROUND:The aim of this study was to detect and characterize Cryptosporidium spp. in water samples collected from recreational ponds of Chaharmahal va Bakhtiyari Province of Iran. METHODS:Thirty water samples were collected from November 2009 to May 2010. Each sample contained 10 liters of water. We used the SSU rR...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2011-03-01 00:00:00
abstract：Background:Cystic echinococcosis can cause severe disease and probable death in humans. Epitopes of its antigens play a key role in the sensitivity and specificity of immunodiagnostic tests. Methods:Epitope prediction software programs predict the most antigenic linear B-cell epitopes of AgB (8 kD), Ag5, and Ag95. Six...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2020-01-01 00:00:00
abstract：BACKGROUND:The leishmanicidal and cytotoxic activity of ten essential oils obtained from ten plant specimens were evaluated. METHODS:Essential oils were obtained by the steam distillation of plant leaves without any prior processing. Cytotoxicity was tested on J774 macrophages and leishmanicidal activity was assessed ...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：BACKGROUND:Pteridine metabolic pathway is unusual features of Leishmania, which is necessary for the growth of parasite. Leishmania has evolved a complex and versatile pteridine salvage network which has the ability of scavenging a wide area of the conjugated and unconjugated pteridines especially folate and biopterin....
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-04-01 00:00:00
abstract：Background:Although rigorous efforts have substantially decreased the malaria burden through decades, it still threatens the lives of millions of children. Development of an effective vaccine can provide important approach in malaria control strategies. Unfortunately, development of an effective vaccine for falciparum ...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章,评审
doi：
更新日期：2018-01-01 00:00:00
abstract：BACKGROUND:Leishmania tropica is a genetically divergent species. Amplification of entire internal transcribed spacer (ITS) region of L. tropica isolates obtained from Bam district, one of the well known focus of anthroponotic cutaneous leishmaniasis (ACL) in Iran, revealed a double-band pattern in agarose gel electrop...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-04-01 00:00:00
abstract：BACKGROUND:Schistosomiasis is a debilitating disease, infects millions of people in tropical and subtropical regions. This study assessed the situation of the urinary schistosomiasis among schoolchildren of the Alsaial Alsagair village, Sudan. METHODS:A cross-sectional survey was carried out in 2016, in the Alsaial Al...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2017-04-01 00:00:00
abstract：BACKGROUND:The aim of this study was to evaluate the antimalarial effects of Iranian flora Artemisia khorassanica against Plasmodium bergheiin vivo and pharmacochemistry of its natural components. METHODS:The aerial parts of Iranian flora A. khorasanica were collected at flowering stage from Khorassan Province, northe...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2010-03-01 00:00:00
abstract：BACKGROUND:Echinococcosis is a zoonotic parasitic disease of humans and various herbivorous domestic animals transmitted by the contact with domestic and wild carnivores, mainly dogs and foxes. The aim of this study is the production, purification and evaluation immunogenicity of new construction of EG95 protein. METH...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2014-10-01 00:00:00
abstract：Background:To assay the Trichinella-specific IgM and IgG antibody responses during the early stage of infection, serum was collected from mice infected with the muscle larvae (ML) of T. spiralis (ISS534) at different dpi (days post infection) up to 60 days. Methods:The levels of IgM and IgG antibodies in serum were me...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2019-04-01 00:00:00
abstract：BACKGROUND:Echinococcus granulosus cultivation is very important for improvement of different aspect of medical and veterinary researches. Despite many advances in this case, there is a missing link for in vitro life cycle of adult worms and it is fertilization. Regarding the researchers' observations, self-fertilizati...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2014-03-01 00:00:00
abstract：Background:Whole killed Leishmania vaccine reached phase III clinical trials but failed to display significant efficacy in human mainly due to limited Th1 inducer adjuvant. Liposomes consisting of 1, 2-dioleoyl-3trimethylammonium-propane (DOTAP) bearing an inherent adjuvanticity and 1, 2-dioleoyl-L-α-glycero-3-phosphat...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2019-01-01 00:00:00
abstract：BACKGROUND:Canine visceral leishmaniasis (CVL) is not only an emerging veterinary concern but also a public health threat in endemic areas. The aim of this study was to assess the efficacy, immunogenicity and safety of two doses of aluminum hydroxide (alum) precipitated Leishmania major (Alum-ALM) mixed with BCG plus i...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2015-07-01 00:00:00
abstract：BACKGROUND:The aim of this study was to investigate the role of oxidative stress in the pathology of ovine dicrocoeliasis. METHODS:During Dec 2013 - Oct 2014, seventy-two sheep (1-3 years) with liver dicrocoeliasis along with 47 healthy sheep were selected from animals admitted for slaughtering at slaughterhouse locat...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2017-01-01 00:00:00
abstract：BACKGROUND:A successful malaria elimination program calls for enough attention to parasite carriers, especially asymptomatic malaria, as well as the diagnosis and treatment of clinical cases. Asymptomatic malaria is an infection that patients do not show any symptom; thus, these patients play critical role in the conce...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：BACKGROUND:Plasmodium vivax is the most widespread species of Plasmodium in humans and causing about 80 million clinical cases annually. This study was undertaken to detect P. vivax in asymptomatic treated vivax malaria patients to trace latent/sub-patent malaria infection. METHOD:The venous blood of all detected case...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-10-01 00:00:00
abstract：BACKGROUND:Cryptosporidiosis is one of the most important parasitic infections in human and animals. This study was designed for survey on the prevalence of Cryptosporidium infection in farms of Ilam, west of Iran, using parasitology method and genotyping by Nested PCR-RFLP. METHODS:Fecal samples of 217 cattle were co...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2014-09-01 00:00:00
abstract：BACKGROUND:This study was proposed to monitor the situation of asymptomatic malaria among the native population and Afghani and Pakistani immigrants in Kahnooj and Ghale-Ganj districts from Kerman Province, Southeastern Iran. METHODS:A number of 180 and 120 individuals from Kahnooj and Ghale-Ganj respectively were reg...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2016-04-01 00:00:00
abstract：BACKGROUND:Leishmaniasis is a parasitic disease that appears with a range of symptoms including cutaneous, mucocutaneous, and visceral leishmaniasis. The present study sought to determine the antileishmanial effect of the extract of Artemisia dracunculus (Tarragon) compared to control treatment with pentavalent antimon...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2017-07-01 00:00:00
abstract：:Tenia solium, a parasite causes cysticercous cellulose when affecting the central nervous system, the manifestation is called neurocysticercosis. The most common symptom in neurocysticercosis is seizure. Generally, oral diagnosticians come across cases of oral cysticercosis and it is rare to find a case of neurocystic...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-10-01 00:00:00
abstract：Background:In Iran, both forms of cutaneous (CL) and visceral leishmaniasis (VL) have been re-ported; so the accurate species identification of the parasite(s) and the analysis of genetic diversity are necessary. Methods:The investigation was conducted from 2014 to 2015 in the northwest and south of Iran, where VL is ...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2018-10-01 00:00:00
abstract：BACKGROUND:The objective of this study was to investigate HLA-DRB1*and DQB1* allelic polymorphisms in Iranian patients with hydatidose. This is the first survey dealing with the correlation between HLA-DRB1* and DQB1* alleles and cystic echinococcosis in Iranian patients. METHODS:The study was carried out on 56 patien...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-10-01 00:00:00
abstract：Background:Toxoplasmosis is a common infection all around the world. During pregnancy; it may lead to congenital disorders or abortion in human and animals. Severe damage of toxoplasmosis indicates to require effective vaccine. One of dense granules antigen is GRA4 that secrete from tachyzoite and bradyzoite. GRA4 geno...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2018-07-01 00:00:00
abstract：Background:Intestinal parasitic infections (IPIs) are among the most important etiologies of gastrointestinal disorders in developing countries. The present study was performed to determine the prevalence of IPIs in rural inhabitants of Fouman, northern Iran. Methods:Overall, 31 villages were randomly selected during ...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2020-01-01 00:00:00
abstract：:Cases of Sparganum mansoni, caused by the plerocercoid larva of the tapeworm S. mansoni, occur throughout the world, particularly in Asian, Middle Eastern, and European countries. However, cases of infection with this parasite are rarely seen in Japan. Here, we present a case of a 61-year-old woman with a solitary sub...
journal_title：Iranian journal of parasitology
pub_type：
doi：
更新日期：2012-01-01 00:00:00
abstract：BACKGROUND:Clinical manifestations of Strongyloides stercoralis are variable from asymptomatic to hyperinfection and devastating disseminated infections. Hereby, clinical characteristics of a large series of Iranian strongyloidiasis indigenous cases are described. METHODS:The records of people referred to the Helminth...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2014-04-01 00:00:00
abstract：BACKGROUND:Trichomoniasis is a worldwide protozoan parasitic disease and metronidazole is a choice drug for its treatment. Because of disease importance in public health and its controversial ideas about the prevalence of drug resistance, this study was carried out. METHODS:Fifty-two suspected vaginal samples were col...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2010-12-01 00:00:00
abstract：:Enterobius vermicularis is an intestinal nematode of humans and the most common helminth infection. Main transmission path is direct contact between infected and uninfected person meaning ingestion of the eggs. Human infections are usually asymptomatic or manifest as perianal itching. Although ectopic locations are un...
journal_title：Iranian journal of parasitology
pub_type：
doi：
更新日期：2017-10-01 00:00:00
abstract：BACKGROUND:The number of malaria cases is declining worldwide; however, it remains as a serious health problem. Diagnosing unusual cases is the most important issue to manage the problem. This study designed to describe the number of falciparum and vivax malaria infected patients referred to Malaria Reference Laborator...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2015-10-01 00:00:00
abstract：BACKGROUND:Successful therapy of leishmaniasis depends on effective cellular immune response. We evaluated the effectiveness of sodium selenite and zinc sulphate as known immunomodulator materials, in combination with Glucantime® in treatment of cutaneous leishmaniasis lesions resulting from Leishmania major in suscept...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2010-09-01 00:00:00
