Molecular Genotyping of the Human Cystic Echinococcosis in Mazandaran Province, North of Iran.

Abstract:

Background:The larval stage of the tapeworm (cestode) Echinococcus granulosus is the etiological agent of hydatidosis or cystic echinococcosis, which is the zoonotic parasitic disease causing morbidity and mortality in both humans and livestock. Due to a lack of accurate data on the human isolates of E. granulosus in Mazandaran Province, northern Iran, the current study aimed to survey the population genetic pattern of cystic echinococcosis isolated from humans by sequencing the mitochondrial genes of NADH dehydrogenase subunit 1 (nad1). Methods:Overall, 47 formalin fixed paraffin-embedded tissue (FFPT) blocks were collected from patients' files in various pathology departments of Mazandaran Province in Iran from 2003 to 2015. PCR was performed to amplify a 398bp DNA fragment of mitochondrial nad1. PCR products were sequenced by Bioneer Corporation (South Korea), and the resulting data were analyzed via relevant software to determine the genotypes. Results:The nad1 gene was successfully amplified on 10 from all of the E. granulosus isolates. Overall, 66.6% and 33.3% of the isolates in the studied area displayed the G1 and G2-G3 genotypes, respectively. Conclusion:This study may provide the foundation for further studies in revealing the regional transmission patterns and also in designing adequate control procedures.

journal_name

Iran J Parasitol

authors

Hedayati Z,Daryani A,Sarvi S,Gholami S,Sharif M,Pirestani M,Dodangeh S,Bari S,Gholami S,Mizani A,Hosseini SA

subject

Has Abstract

pub_date

2019-01-01 00:00:00

pages

151-158

issue

1

eissn

1735-7020

issn

2008-238X

journal_volume

14

pub_type

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