Abstract:
Background:Whole killed Leishmania vaccine reached phase III clinical trials but failed to display significant efficacy in human mainly due to limited Th1 inducer adjuvant. Liposomes consisting of 1, 2-dioleoyl-3trimethylammonium-propane (DOTAP) bearing an inherent adjuvanticity and 1, 2-dioleoyl-L-α-glycero-3-phosphatidylethanolamine (DOPE) is well known to intensify the efficacy of positively charged liposomes. Methods:Soluble Leishmania major antigens (SLA) encapsulated in cationic liposomes using lipid film method in 2016). BALB/c mice were immunized subcutaneously (SC), three times in a 2-wk interval, with Lip (DOTAP)-SLA+, Lip (DOTAP/DOPE)-SLA+, Lip (DOTAP/DOPE/CHO)-SLA+, Lip (DOTAP/DOPE/CHO), Lip (DOPE/CHO), SLA or HEPES buffer. At week 2 after the last booster injection, immunized mice have challenged SC in the footpad with L. major parasites. To investigate the rate of protection and the type of immune response generated in mice, lesions development was assessed, IL-4 and IFN-γ levels with the ratio of IgG2a/IgG1 isotype were studied to describe the type of generated immune response. Results:Mice immunized with all liposomal form of SLA showed smaller footpad swelling and lower parasite burden in the spleen and footpad compared to the group of mice received buffer. However, these formulations did not show protection against leishmaniosis because of a generated mixed Th1/Th2 response in mice characterized by high production of IFN-γ and IL4 and a high titer of IgG1 and IgG2a antibody. Conclusion:Immunization with Lip (DOTAP/DOPE/CHO)-SLA+ was not an appropriate strategy to protect mice against leishmaniosis.
journal_name
Iran J Parasitoljournal_title
Iranian journal of parasitologyauthors
Hojatizade M,Badiee A,Khamesipour A,Jaafari MRsubject
Has Abstractpub_date
2019-01-01 00:00:00pages
68-77issue
1eissn
1735-7020issn
2008-238Xjournal_volume
14pub_type
杂志文章abstract:BACKGROUND:Echinococcus granulosus cultivation is very important for improvement of different aspect of medical and veterinary researches. Despite many advances in this case, there is a missing link for in vitro life cycle of adult worms and it is fertilization. Regarding the researchers' observations, self-fertilizati...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2014-03-01 00:00:00
abstract:BACKGROUND:Plasmodium vivax is the predominant species causes of malaria with about 90% total annual reported malaria in Iran. This study conducted to determine the susceptibility of Plasmodium vivax isolates to chloroquine in Sistan and Balochistan Province, southeastern Iran. METHODS:A total 270 subjects with sympto...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2012-01-01 00:00:00
abstract:Background:Toxoplasmosis is a common infection all around the world. During pregnancy; it may lead to congenital disorders or abortion in human and animals. Severe damage of toxoplasmosis indicates to require effective vaccine. One of dense granules antigen is GRA4 that secrete from tachyzoite and bradyzoite. GRA4 geno...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2018-07-01 00:00:00
abstract::In the urine of a patient with chronic prostatitis, renal microlithiasis and acute cystitis we found the ciliate protozoa Colpoda spp., both in vegetative and cystic form. The entry point was most likely the urinary tract. Keeping in mind that only four more cases of Colpoda spp. existent in human urine have already b...
journal_title:Iranian journal of parasitology
pub_type:
doi:
更新日期:2011-12-01 00:00:00
abstract:BACKGROUND:Echinococcosis is a zoonotic parasitic disease of humans and various herbivorous domestic animals transmitted by the contact with domestic and wild carnivores, mainly dogs and foxes. The aim of this study is the production, purification and evaluation immunogenicity of new construction of EG95 protein. METH...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2014-10-01 00:00:00
abstract::Visceral leishmaniasis is a common parasitic disease between humans and animals, transmitted by sandflies (Phlebotomus) in the Mediterranean countries, including Iran. The statistics have been reported less than real due to errors in the diagnosis and reporting of affected cases. In this report, we will present the sy...
journal_title:Iranian journal of parasitology
pub_type:
doi:
更新日期:2020-04-01 00:00:00
abstract:BACKGROUND:Coccidiosis is a serious protozoal disease of poultry. The identification of Eimeria species has important implications for diagnosis and control as well as for epidemiology. The molecular characterization of Eimeria species infecting Egyptian baladi chickens was investigated. METHODS:Eimeria species oocyst...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Due to the lack of a suitable and economic test for the analysis of the polymorphism at codon 167, we developed a new PCR-RFLP technique, based on a modified forward primer (UT-HC167 MF-primer), to identify simultaneously the SNPs at codons 167 and 200 of isotype 1 β-tubulin gene of Haemonchus contortus. ME...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:The incidence of cutaneous leishmaniasis in the city of Mehran has risen sharply in recent years because the city borders Iraq, which has allowed entrance of different Leishmania strains. These strains have different shapes, periods of disease, and healing of lesions. The present study identified and determi...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2016-01-01 00:00:00
abstract:Background:Due to the limited number of the available drugs for the treatment of trypanosomiasis, this study was designed to evaluate the trypanocidal effects of cisplatin or/and Nigella sativa oil (NSO) in experimentally infected mice with T. evansi. Methods:During 2015 at the Parasitology Department, Menoufia Univer...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Leishmania tropica is a genetically divergent species. Amplification of entire internal transcribed spacer (ITS) region of L. tropica isolates obtained from Bam district, one of the well known focus of anthroponotic cutaneous leishmaniasis (ACL) in Iran, revealed a double-band pattern in agarose gel electrop...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2013-04-01 00:00:00
abstract:BACKGROUND:This study was proposed to monitor the situation of asymptomatic malaria among the native population and Afghani and Pakistani immigrants in Kahnooj and Ghale-Ganj districts from Kerman Province, Southeastern Iran. METHODS:A number of 180 and 120 individuals from Kahnooj and Ghale-Ganj respectively were reg...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2016-04-01 00:00:00
abstract:BACKGROUND:Coccidiosis of domestic fowl, caused by species of the Genus Eimeria, is responsible for important economic losses in poultry production. Because different species and/or strains can vary in pathogenicity and other biological parameters, their precise characterization is important for epizootiological studie...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2011-12-01 00:00:00
abstract:BACKGROUND:Neospora caninum, an obligate intracellular protozoan parasite, is recognized as a major cause of abortion in cattle, while limited information is presently available on the seroprevalence of Neospora antibodies in horses' worldwide. The aim of the present study was to determine serologic prevalence of Neosp...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2011-06-01 00:00:00
abstract:BACKGROUND:Hydatidosis, as the most important zoonotic parasitic disease in Iran, has posed many health and economic losses. This study was conducted to investigate the demographic characteristics of hydatid cyst surgeries in hospitals of East Azerbaijan Province, Northwest of Iran. METHODS:Demographic characteristics...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2014-04-01 00:00:00
abstract::The presence of Visceral Larva Migrans (VLM) in a patient is reported. A 57-year- old woman suffering from right upper abdominal and suprapubic pain referred into a clinic in Khorramabad, Lorestan Province, Iran. A cystoscopy was performed and biopsy was taken. The light microscopic study showed a couple of larvae as ...
journal_title:Iranian journal of parasitology
pub_type:
doi:
更新日期:2011-03-01 00:00:00
abstract::Herein, a 28-year-old man with hoarseness, skin and oral lesions is presented. At the time of admission, the patient had an erythematous plaque on his chin near his lower lip and an erythematous-violaceous plaque on his palate near the opening of the pharynx and 20 kg weight lost in last one year. The biopsy of his sk...
journal_title:Iranian journal of parasitology
pub_type:
doi:
更新日期:2015-04-01 00:00:00
abstract:Background:The Totiviridae family includes a number of double-stranded RNA viruses that can infect Trichomonas vaginalis. Some T. vaginalis isolates are infected with one or more double-stranded RNA (dsRNA) viruses. In this study, different strains of double-stranded RNA virus in Iranian isolates of T. vaginalis were e...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:10.18502/ijpa.v15i3.4200
更新日期:2020-07-01 00:00:00
abstract:BACKGROUND:Successful therapy of leishmaniasis depends on effective cellular immune response. We evaluated the effectiveness of sodium selenite and zinc sulphate as known immunomodulator materials, in combination with Glucantime® in treatment of cutaneous leishmaniasis lesions resulting from Leishmania major in suscept...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2010-09-01 00:00:00
abstract:BACKGROUND:Leishmania Homologue of receptors for Activated C Kinase (LACK) antigen is a 36-kDa protein, which provokes a very early immune response against Leishmania infection. There are several reports on the expression of LACK through different life-cycle stages of genus Leishmania, but only a few of them have focus...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2014-10-01 00:00:00
abstract:BACKGROUND:Schistosomiasis is a debilitating disease, infects millions of people in tropical and subtropical regions. This study assessed the situation of the urinary schistosomiasis among schoolchildren of the Alsaial Alsagair village, Sudan. METHODS:A cross-sectional survey was carried out in 2016, in the Alsaial Al...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2017-04-01 00:00:00
abstract:BACKGROUND:This study was conducted to collect informative data on the parasitic infection of wild rodents, emphasizing on finding parasites, which have medical importance to human. METHODS:During 2012-2014, a total number of 91 wild rodents were captured from rural areas of Turkmen Sahra, Golestan Province, using han...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2016-07-01 00:00:00
abstract:Background:Intestinal parasites are one of the health challenges in developing countries. Decreasing the prevalence of intestinal parasitic infections (IPIs) is one of the main aims of health services in these countries. This study was designed to determine the current status of IPIs in rural residents of Takestan a to...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2019-10-01 00:00:00
abstract:Background:This cross-sectional investigation aimed to evaluate the prevalence of IgM and IgG anti-Toxoplasma gondii antibodies and the associated risk factors among healthy blood donors in Khorasan Razavi Province, northeast of Iran from Nov 2014 to May 2015. Methods:Overall, 491 serum samples from apparently healthy...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2017-10-01 00:00:00
abstract:BACKGROUND:The sole published data on feline heartworm infection in the Philippines was reported four decades ago. The study therefore endeavoured to assess and provide an update on the current status of heartworm infection in domesticated feline species using serologic and parasitological examination methods. METHODS...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:The number of malaria cases is declining worldwide; however, it remains as a serious health problem. Diagnosing unusual cases is the most important issue to manage the problem. This study designed to describe the number of falciparum and vivax malaria infected patients referred to Malaria Reference Laborator...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2015-10-01 00:00:00
abstract:BACKGROUND:Vast majority of complaints and physical examination findings of hydatid disease are common in emergency room patients. Different emergency presentations of hydatid cyst disease and their treatment are evaluated. We studied preoperative laboratory findings of these patients to identify any parameters to pred...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2016-10-01 00:00:00
abstract:BACKGROUND:We evaluated the in vivo activity of Bunium persicum (Boiss) essential oil on infected mice with acute toxoplasmosis. METHODS:To evaluate prophylactic effects, male NMRI mice received B. persicum essential oil at the concentrations of 0.05 and 0.1 mL/kg for 14 days. After 24 h mice were infected intraperito...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2015-10-01 00:00:00
abstract:Background:Soil-transmitted helminth infections constitute a public health problem in the rural areas of tropical and subtropical regions, including Thailand. We aimed to determine the prevalence of soil-transmitted helminth infections and underlying risk factors among the schoolchildren living in the rural areas of so...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:10.18502/ijpa.v15i3.4210
更新日期:2020-07-01 00:00:00
abstract:BACKGROUND:Clinical manifestations of Strongyloides stercoralis are variable from asymptomatic to hyperinfection and devastating disseminated infections. Hereby, clinical characteristics of a large series of Iranian strongyloidiasis indigenous cases are described. METHODS:The records of people referred to the Helminth...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2014-04-01 00:00:00