Abstract:
Background:Although Plasmodium vivax is usually known as benign malaria, some variations of the parasite can result in acute and sever infection. In this study we tried to determine some genetic variations in PvAMA-1 antigen among the samples were collected form southeastern Iran. Methods:About two ml blood samples were collected into EDTA pre-dosed tubes from 30 P. vivax-infected patients individually between 2011 and 2013. A Giemsa stained thick and thin blood film was prepared from each of the patients. A PCR-RFLP technique was employed using EcoR-1, Pvu-II and Hind3 restriction enzymes to determine the allelic variations of the antigen. Results:A 1300bp gene corresponding to PvAMA-1 was selected for the amplification process. Among the total cases identified in this study 90% showed similar bounds when exposed to the restriction enzymes. Nine isolates (accession numbers: KF435081-KF435083 and JF682785-JF682790) were identified and registered in Gene bank. Identity among isolates was more than 96% in nucleotide level. Dendrogram clarified a close relationship among the clusters in spite of geographical distribution of the parasite. Conclusion:This study increased our data about prevalence and variation of PvAMA-1 alleles amongst P. vivax isolates in southeastern parts of Iran where besides native population bears considerable Afghan and Pakistani immigrants.
journal_name
Iran J Parasitoljournal_title
Iranian journal of parasitologyauthors
Motevalli Haghi A,Moradi S,Nateghpour M,Edrissian Gsubject
Has Abstractpub_date
2018-07-01 00:00:00pages
473-479issue
3eissn
1735-7020issn
2008-238Xjournal_volume
13pub_type
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doi:
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doi:
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pub_type: 杂志文章
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更新日期:2015-04-01 00:00:00
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doi:
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journal_title:Iranian journal of parasitology
pub_type: 杂志文章
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