Abstract：
BACKGROUND:Cutaneous Leishmaniasis (CL) is a parasitic skin disease. Diagnosis primarily is based on clinical signs and microscopic observation of parasite on direct stained smears or tissue sections. Sensitivity of direct smear is not as high as molecular methods. The aim of this study was to identify and characterize Leishmania species among the negative direct smears obtained from skin ulcers suspected to CL by PCR method. METHODS:Among 81 patients with suspicious skin lesions to CL referred to the Parasitology lab, negative Giemsa stained smears were collected. DNA extraction performed by scraping stained smears, then PCR was performed. RESULTS:Among the DNA extracted from smears, L. tropica was isolated from 9 (11.1%) of the smears and L.major was not isolated from any samples. CONCLUSION:Direct microscopy on stained smears for diagnosis of leishmaniasis is not enough accurate. PCR is recommended for clinically suspected lesions with negative result of direct smear.
journal_name
Iran J Parasitoljournal_title
Iranian journal of parasitologyauthors
Mohaghegh M,Fata A,Salehi G,Berenji F,Bazzaz MM,Rafatpanah H,Parian M,Movahedi Asubject
Has Abstractpub_date
2013-04-01 00:00:00pages
337-41issue
2eissn
1735-7020issn
2008-238Xjournal_volume
8pub_type
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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更新日期：2020-04-01 00:00:00
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journal_title：Iranian journal of parasitology
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doi：
更新日期：2012-01-01 00:00:00
abstract：BACKGROUND:Leishmaniasis is a parasitic infection that may lead to a variety of manifestations. In Iran, cutaneous leishmaniasis (CL) has a high prevalence. There are many treatment modalities for CL. The use of oral terbinafine in the treatment of CL has recently been considered. The aim of this study was to compare c...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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更新日期：2019-04-01 00:00:00
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journal_title：Iranian journal of parasitology
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更新日期：2017-10-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：10.18502/ijpa.v15i3.4210
更新日期：2020-07-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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更新日期：2010-12-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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更新日期：2010-03-01 00:00:00
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journal_title：Iranian journal of parasitology
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更新日期：2011-12-01 00:00:00
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journal_title：Iranian journal of parasitology
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doi：
更新日期：2018-04-01 00:00:00
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journal_title：Iranian journal of parasitology
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更新日期：2014-09-01 00:00:00
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journal_title：Iranian journal of parasitology
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doi：
更新日期：2019-01-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2014-10-01 00:00:00
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journal_title：Iranian journal of parasitology
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doi：
更新日期：2020-04-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2017-01-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2010-09-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2016-04-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2014-10-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-10-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-04-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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更新日期：2019-07-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2016-10-01 00:00:00
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journal_title：Iranian journal of parasitology
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更新日期：2014-09-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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更新日期：2013-01-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2014-04-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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更新日期：2020-01-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type：
doi：
更新日期：2011-03-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章,评审
doi：
更新日期：2017-07-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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更新日期：2019-10-01 00:00:00
