Abstract:
BACKGROUND:Fasciolosis in livestock is a crucial concern in the globe, mainly due to its impact on human health. The aim of this study was to determine the rate of infection with Fasciola gigantica (Cobbold, 1855) larvae in the field-collected snails of Lymnaea auricularia (Linnaeus, 1785) from northwestern Iran using a molecular approach. METHODS:A total of 6,759 pond snails were collected from 28 freshwater bodies in West Azarbaijan. PCR was performed to amplify a 618-bp fragment of the nuclear 28 SrRNA gene of Fasciola. The PCR products were digested by AvaII restriction enzyme to create restriction fragment length polymorphism (RFLP) patterns specific for the detection of F. gigantica. RESULTS:Of the total collected snails 496 (7.34 %) were L. auricularia, among which 4.64% (23 out of 496) were infected with a Fasciola species according to the PCR analysis. Only 2.22% (11 out of 496) of the infected snails were from the mountainous areas. The highest Fasciola infection rate recorded in the snails of a single site was 1.81% (9 out of 496 snails). Based on the RFLP pattern, F. gigantica accounted for 2.42% of the infection rates in the study sites. CONCLUSION:Application of PCR-RFLP was proven to be a useful approach for valid and robust detection of the infection with F. gigantica in its intermediate host snails. These findings may therefore be applicable for establishment of the control programs against dissemination of the infection in different regions.
journal_name
Iran J Parasitoljournal_title
Iranian journal of parasitologyauthors
Yakhchali M,Malekzadeh-Viayeh R,Imani-Baran Asubject
Has Abstractpub_date
2014-09-01 00:00:00pages
358-64issue
3eissn
1735-7020issn
2008-238Xjournal_volume
9pub_type
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journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Cryptosporidiosis is one of the most important parasitic infections in human and animals. This study was designed for survey on the prevalence of Cryptosporidium infection in farms of Ilam, west of Iran, using parasitology method and genotyping by Nested PCR-RFLP. METHODS:Fecal samples of 217 cattle were co...
journal_title:Iranian journal of parasitology
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journal_title:Iranian journal of parasitology
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journal_title:Iranian journal of parasitology
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journal_title:Iranian journal of parasitology
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journal_title:Iranian journal of parasitology
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journal_title:Iranian journal of parasitology
pub_type: 杂志文章
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更新日期:2012-01-01 00:00:00
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journal_title:Iranian journal of parasitology
pub_type: 杂志文章
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journal_title:Iranian journal of parasitology
pub_type: 杂志文章
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journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2017-01-01 00:00:00
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journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2010-06-01 00:00:00
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journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2014-03-01 00:00:00
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journal_title:Iranian journal of parasitology
pub_type:
doi:
更新日期:2016-01-01 00:00:00
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journal_title:Iranian journal of parasitology
pub_type:
doi:
更新日期:2011-12-01 00:00:00
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journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2019-01-01 00:00:00
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journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
abstract::Enterobius vermicularis is an intestinal nematode of humans and the most common helminth infection. Main transmission path is direct contact between infected and uninfected person meaning ingestion of the eggs. Human infections are usually asymptomatic or manifest as perianal itching. Although ectopic locations are un...
journal_title:Iranian journal of parasitology
pub_type:
doi:
更新日期:2017-10-01 00:00:00
abstract:BACKGROUND:Echinococcosis is a zoonotic parasitic disease of humans and various herbivorous domestic animals transmitted by the contact with domestic and wild carnivores, mainly dogs and foxes. The aim of this study is the production, purification and evaluation immunogenicity of new construction of EG95 protein. METH...
journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2014-10-01 00:00:00
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journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
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journal_title:Iranian journal of parasitology
pub_type: 杂志文章,评审
doi:
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journal_title:Iranian journal of parasitology
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更新日期:2012-01-01 00:00:00
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journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2016-10-01 00:00:00
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journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2017-10-01 00:00:00
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journal_title:Iranian journal of parasitology
pub_type:
doi:
更新日期:2019-07-01 00:00:00
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journal_title:Iranian journal of parasitology
pub_type:
doi:
更新日期:2015-04-01 00:00:00
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journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2014-04-01 00:00:00
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journal_title:Iranian journal of parasitology
pub_type: 杂志文章
doi:
更新日期:2013-10-01 00:00:00
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journal_title:Iranian journal of parasitology
pub_type: 杂志文章
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