Abstract：
BACKGROUND:Leishmania Homologue of receptors for Activated C Kinase (LACK) antigen is a 36-kDa protein, which provokes a very early immune response against Leishmania infection. There are several reports on the expression of LACK through different life-cycle stages of genus Leishmania, but only a few of them have focused on L.tropica. METHODS:The present study provides details of the cloning, DNA sequencing and gene expression of LACK in this parasite species. First, several local isolates of Leishmania parasites were typed in our laboratory using PCR technique to verify of Leishmania parasite species. After that, LACK gene was amplified and cloned into a vector for sequencing. Finally, the expression of this molecule in logarithmic and stationary growth phase promastigotes, as well as in amastigotes, was evaluated by Reverse Transcription-PCR (RT-PCR) technique. RESULTS:The typing result confirmed that all our local isolates belong to L.tropica. LACK gene sequence was determined and high similarity was observed with the sequences of other Leishmania species. Furthermore, the expression of LACK gene in both promastigotes and amastigotes forms was confirmed. CONCLUSION:Overall, the data set the stage for future studies of the properties and immune role of LACK gene products.
journal_name
Iran J Parasitoljournal_title
Iranian journal of parasitologyauthors
Hammoudeh N,Kweider M,Abbady AQ,Soukkarieh Csubject
Has Abstractpub_date
2014-10-01 00:00:00pages
574-83issue
4eissn
1735-7020issn
2008-238Xjournal_volume
9pub_type
杂志文章abstract：BACKGROUND:Cryptosporidiosis is one of the most important parasitic infections in human and animals. This study was designed for survey on the prevalence of Cryptosporidium infection in farms of Ilam, west of Iran, using parasitology method and genotyping by Nested PCR-RFLP. METHODS:Fecal samples of 217 cattle were co...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2014-09-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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更新日期：2020-04-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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更新日期：2015-10-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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journal_title：Iranian journal of parasitology
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journal_title：Iranian journal of parasitology
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更新日期：2015-01-01 00:00:00
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journal_title：Iranian journal of parasitology
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更新日期：2011-12-01 00:00:00
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journal_title：Iranian journal of parasitology
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更新日期：2011-06-01 00:00:00
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journal_title：Iranian journal of parasitology
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更新日期：2011-03-01 00:00:00
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journal_title：Iranian journal of parasitology
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doi：
更新日期：2016-07-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2014-03-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2018-01-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type：
doi：
更新日期：2020-04-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2016-04-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2016-01-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type：
doi：
更新日期：2019-07-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2015-07-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章,评审
doi：
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2019-04-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2014-03-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2017-01-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-04-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2010-03-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2010-06-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
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