Abstract：
BACKGROUND:Echinococcus granulosus cultivation is very important for improvement of different aspect of medical and veterinary researches. Despite many advances in this case, there is a missing link for in vitro life cycle of adult worms and it is fertilization. Regarding the researchers' observations, self-fertilization can be done in worms living in dog intestine, but despite all sorts of experimental techniques, this phenomenon has never been observed in reared worms in culture media. Furthermore, cross fertilization has not been observed in vitro and even in parasites with dog intestinal origin; although it theoretically is possible. During a follow-up of cultivated adult worms, evidences of behaviors similar to self-mating (Type 2) and cross-mating were observed in our lab which will be presented here. METHODS:Protoscoleces were aseptically removed from sheep hydatid cysts, washed twice with PBS and then cultivated in S.10E.H culture medium. The stages of parasite growth were observed using an inverted microscope for two months and all stages and behaviors were microscopically photographed. Different movies have also been made from these behavioral features. RESULTS:After around 55 days post cultivation, some evidences of behaviors similar to self-mating (Type 2) and cross-mating were observed in some of the mature adult worms. However, fertile eggs in these parasites have never been observed. CONCLUSION:Regarding the above observations, these parasites show tendency to unsuccessful self-mating/fertilization (type 2) which failure could be due to anatomical position and physiological maturation. Also lack of suitable conditions for self-fertilization causes the worms try to do unsuccessful cross- mating/fertilization in culture media.
journal_name
Iran J Parasitoljournal_title
Iranian journal of parasitologyauthors
Mohammadzadeh T,Sadjjadi SM,Rahimi Hsubject
Has Abstractpub_date
2014-03-01 00:00:00pages
129-33issue
1eissn
1735-7020issn
2008-238Xjournal_volume
9pub_type
杂志文章abstract：BACKGROUND:As for human trichomoniasis the host-parasite relationship is very complex, and the broad ranges of clinical symptoms are unlikely be attributable to a single pathogenic mechanism. Specific Random Amplified Polymorphic DNA (RAPD) markers of 490 bp, 720 bp and 460 bp using the primers Tv-5, OPA-6 and OPA-11, ...
journal_title：Iranian journal of parasitology
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journal_title：Iranian journal of parasitology
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journal_title：Iranian journal of parasitology
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更新日期：2020-01-01 00:00:00
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journal_title：Iranian journal of parasitology
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更新日期：2015-01-01 00:00:00
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journal_title：Iranian journal of parasitology
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journal_title：Iranian journal of parasitology
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更新日期：2016-04-01 00:00:00
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journal_title：Iranian journal of parasitology
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更新日期：2013-07-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2015-10-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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journal_title：Iranian journal of parasitology
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pub_type： 杂志文章
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更新日期：2018-07-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-10-01 00:00:00
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journal_title：Iranian journal of parasitology
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更新日期：2017-04-01 00:00:00
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journal_title：Iranian journal of parasitology
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更新日期：2010-12-01 00:00:00
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journal_title：Iranian journal of parasitology
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journal_title：Iranian journal of parasitology
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更新日期：2013-04-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
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