Abstract：
BACKGROUND:Due to the lack of a suitable and economic test for the analysis of the polymorphism at codon 167, we developed a new PCR-RFLP technique, based on a modified forward primer (UT-HC167 MF-primer), to identify simultaneously the SNPs at codons 167 and 200 of isotype 1 β-tubulin gene of Haemonchus contortus. METHODS:There already are several safe and easy methods for identification of point mutations at codons 198 and 200. Due to the lack of a reliable and easy method for the detection of the single nucleotide polymorphism (SNP) at codon 167, we developed an innovative PCR-RFLP technique based on a modified forward primer (UT-HC167 MF-primer), in which the nucleotide T at the position 443 was substituted through a nucleotide A creating a restriction site for restriction endonuclease SnaB I in the nucleotide sequences including codon 167. A total of 138 adult male H. contortus were collected from three different geo-climatic areas of Iran. The isolated genomic DNA of each single worm was amplified by PCR using primers flanking codon 167. The PCR product (527 bp) was then amplified by semi-nested PCR using the UT-HC167 MF-primer and the reverse primer achieving a PCR product of 451 bp in length. This PCR product was subsequently digested with the restriction endonucleases SnaB I and TaaI for analysis of the mutations at codons 167 and 200, respectively. RESULTS:All worms had two alleles encoding for phenylalanine (BZ(ss) homozygote) for both codons. CONCLUSION:Using the UT-HC167 MF-primer and a suitable reverse primer designed upstream from codon 200, it is possible to amplify a PCR product which can be used for analysis of the SNPs at all three mentioned codons using RFLP.
journal_name
Iran J Parasitoljournal_title
Iranian journal of parasitologyauthors
Shokrani H,Shayan P,Eslami A,Nabavi Rsubject
Has Abstractpub_date
2012-01-01 00:00:00pages
41-8issue
4eissn
1735-7020issn
2008-238Xjournal_volume
7pub_type
杂志文章abstract：BACKGROUND:The aim of this study was to evaluate the antimalarial effects of Iranian flora Artemisia khorassanica against Plasmodium bergheiin vivo and pharmacochemistry of its natural components. METHODS:The aerial parts of Iranian flora A. khorasanica were collected at flowering stage from Khorassan Province, northe...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2010-03-01 00:00:00
abstract：BACKGROUND:Leishmaniasis is a group of diseases that are created by intracellular parasites of Leishmania. Cutaneous leishmaniasis is considered as one of the health problems in some provinces of Iran. METHODS:In this study, a total of 178 Giemsa-stained slides from confirmed cases of cutaneous leishmaniasis were exam...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-07-01 00:00:00
abstract：Background:In Iran, both forms of cutaneous (CL) and visceral leishmaniasis (VL) have been re-ported; so the accurate species identification of the parasite(s) and the analysis of genetic diversity are necessary. Methods:The investigation was conducted from 2014 to 2015 in the northwest and south of Iran, where VL is ...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2018-10-01 00:00:00
abstract：BACKGROUND:The intraperitoneal injection is a common method for establishing the experimental animal model infected with parasites. The aim of this study was to investigate if the intraperitoneal injection was another route of Trichinella spiralis infection. METHODS:From June to July 2015, twenty BALB/c mice were intr...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2017-01-01 00:00:00
abstract：Background:Due to the limited number of the available drugs for the treatment of trypanosomiasis, this study was designed to evaluate the trypanocidal effects of cisplatin or/and Nigella sativa oil (NSO) in experimentally infected mice with T. evansi. Methods:During 2015 at the Parasitology Department, Menoufia Univer...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2018-01-01 00:00:00
abstract：BACKGROUND:Cryptosporidiosis is one of the most important parasitic infections in human and animals. This study was designed for survey on the prevalence of Cryptosporidium infection in farms of Ilam, west of Iran, using parasitology method and genotyping by Nested PCR-RFLP. METHODS:Fecal samples of 217 cattle were co...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2014-09-01 00:00:00
abstract：BACKGROUND:Parasitological methods for the diagnosis of Visceral leishmaniasis (VL) require invasive procedures, so serological and molecular approaches have been developed but are not generally applicable in the field. We evaluated a loop mediated isothermal amplification (LAMP) assay using blood from VL patients and ...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2014-03-01 00:00:00
abstract：BACKGROUND:The objective of this study was to investigate HLA-DRB1*and DQB1* allelic polymorphisms in Iranian patients with hydatidose. This is the first survey dealing with the correlation between HLA-DRB1* and DQB1* alleles and cystic echinococcosis in Iranian patients. METHODS:The study was carried out on 56 patien...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-10-01 00:00:00
abstract：BACKGROUND:This study was conducted to collect informative data on the parasitic infection of wild rodents, emphasizing on finding parasites, which have medical importance to human. METHODS:During 2012-2014, a total number of 91 wild rodents were captured from rural areas of Turkmen Sahra, Golestan Province, using han...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2016-07-01 00:00:00
abstract：BACKGROUND:The aim of this study was to detect and characterize Cryptosporidium spp. in water samples collected from recreational ponds of Chaharmahal va Bakhtiyari Province of Iran. METHODS:Thirty water samples were collected from November 2009 to May 2010. Each sample contained 10 liters of water. We used the SSU rR...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2011-03-01 00:00:00
abstract：BACKGROUND:Coccidiosis of domestic fowl, caused by species of the Genus Eimeria, is responsible for important economic losses in poultry production. Because different species and/or strains can vary in pathogenicity and other biological parameters, their precise characterization is important for epizootiological studie...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2011-12-01 00:00:00
abstract：BACKGROUND:Visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL) are important public health problems in Iran. We aimed to evaluate the diagnostic potential of Western blot (WB) compared with indirect immunofluorescence test (IFAT) to serodiagnosis of leishmaniasis. METHODS:This study was performed from 2010-20...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2017-04-01 00:00:00
abstract：BACKGROUND:Schistosomiasis is a debilitating disease, infects millions of people in tropical and subtropical regions. This study assessed the situation of the urinary schistosomiasis among schoolchildren of the Alsaial Alsagair village, Sudan. METHODS:A cross-sectional survey was carried out in 2016, in the Alsaial Al...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2017-04-01 00:00:00
abstract：:Tenia solium, a parasite causes cysticercous cellulose when affecting the central nervous system, the manifestation is called neurocysticercosis. The most common symptom in neurocysticercosis is seizure. Generally, oral diagnosticians come across cases of oral cysticercosis and it is rare to find a case of neurocystic...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-10-01 00:00:00
abstract：Background:Toxoplasmosis is a common infection all around the world. During pregnancy; it may lead to congenital disorders or abortion in human and animals. Severe damage of toxoplasmosis indicates to require effective vaccine. One of dense granules antigen is GRA4 that secrete from tachyzoite and bradyzoite. GRA4 geno...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2018-07-01 00:00:00
abstract：BACKGROUND:Leishmaniasis is a sand fly-borne disease caused by the protozoan parasites belonging to the genus Leishmania. Because of the preventing and controlling methods, clinical course, prognosis and choice of treatment are differing from species; differentiation of species is critical. The present study was aimed ...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2016-10-01 00:00:00
abstract：BACKGROUND:Trichomoniasis is a worldwide protozoan parasitic disease and metronidazole is a choice drug for its treatment. Because of disease importance in public health and its controversial ideas about the prevalence of drug resistance, this study was carried out. METHODS:Fifty-two suspected vaginal samples were col...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2010-12-01 00:00:00
abstract：:We report a case of human nasal nosocomial myiasis in a 69-year-old rural man with numerous live maggots in nostrils. The patient was admitted in Emergency Ward due to dyspnea. After 72 hours the companion of patient discovered larva. The presence of the third instar larva indicated that the infestation was not more t...
journal_title：Iranian journal of parasitology
pub_type：
doi：
更新日期：2012-01-01 00:00:00
abstract：BACKGROUND:Plasmodium vivax is the predominant species causes of malaria with about 90% total annual reported malaria in Iran. This study conducted to determine the susceptibility of Plasmodium vivax isolates to chloroquine in Sistan and Balochistan Province, southeastern Iran. METHODS:A total 270 subjects with sympto...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：BACKGROUND:To control avian coccidiosis with drug-independent strategy effectively and safely, multivalent hyperimmune egg yolk immunoglobulin (IgY) was prepared and its ability to protect against Eimeria tenella infection was evaluated. METHODS:Hens were orally immunized with live oocysts of 5 species of Eimeria for ...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-07-01 00:00:00
abstract：BACKGROUND:Cutaneous Leishmaniasis (CL) is a parasitic skin disease. Diagnosis primarily is based on clinical signs and microscopic observation of parasite on direct stained smears or tissue sections. Sensitivity of direct smear is not as high as molecular methods. The aim of this study was to identify and characterize...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-04-01 00:00:00
abstract：BACKGROUND:Leishmania Homologue of receptors for Activated C Kinase (LACK) antigen is a 36-kDa protein, which provokes a very early immune response against Leishmania infection. There are several reports on the expression of LACK through different life-cycle stages of genus Leishmania, but only a few of them have focus...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2014-10-01 00:00:00
abstract：BACKGROUND:Acanthamoeba is an opportunistic amphizoic protozoan found in different water sources including swimming pool as well as in sewage. The aim of this study was to investigate the prevalence of Acanthamoeba in tap-water samples in Iran. METHOD:In this descriptive cross-sectional study, 94 samples of cold and w...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2010-06-01 00:00:00
abstract：BACKGROUND:Neospora caninum, an obligate intracellular protozoan parasite, is recognized as a major cause of abortion in cattle, while limited information is presently available on the seroprevalence of Neospora antibodies in horses' worldwide. The aim of the present study was to determine serologic prevalence of Neosp...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2011-06-01 00:00:00
abstract：BACKGROUND:As for human trichomoniasis the host-parasite relationship is very complex, and the broad ranges of clinical symptoms are unlikely be attributable to a single pathogenic mechanism. Specific Random Amplified Polymorphic DNA (RAPD) markers of 490 bp, 720 bp and 460 bp using the primers Tv-5, OPA-6 and OPA-11, ...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2015-07-01 00:00:00
abstract：BACKGROUND:Leishmaniasis is a parasitic infection that may lead to a variety of manifestations. In Iran, cutaneous leishmaniasis (CL) has a high prevalence. There are many treatment modalities for CL. The use of oral terbinafine in the treatment of CL has recently been considered. The aim of this study was to compare c...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
abstract：BACKGROUND:The sole published data on feline heartworm infection in the Philippines was reported four decades ago. The study therefore endeavoured to assess and provide an update on the current status of heartworm infection in domesticated feline species using serologic and parasitological examination methods. METHODS...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：BACKGROUND:Leishmania tropica is a genetically divergent species. Amplification of entire internal transcribed spacer (ITS) region of L. tropica isolates obtained from Bam district, one of the well known focus of anthroponotic cutaneous leishmaniasis (ACL) in Iran, revealed a double-band pattern in agarose gel electrop...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-04-01 00:00:00
abstract：Background:To assay the Trichinella-specific IgM and IgG antibody responses during the early stage of infection, serum was collected from mice infected with the muscle larvae (ML) of T. spiralis (ISS534) at different dpi (days post infection) up to 60 days. Methods:The levels of IgM and IgG antibodies in serum were me...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2019-04-01 00:00:00
abstract：Background:The larval stage of the tapeworm (cestode) Echinococcus granulosus is the etiological agent of hydatidosis or cystic echinococcosis, which is the zoonotic parasitic disease causing morbidity and mortality in both humans and livestock. Due to a lack of accurate data on the human isolates of E. granulosus in M...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2019-01-01 00:00:00
