Abstract：
BACKGROUND:Parasitological methods for the diagnosis of Visceral leishmaniasis (VL) require invasive procedures, so serological and molecular approaches have been developed but are not generally applicable in the field. We evaluated a loop mediated isothermal amplification (LAMP) assay using blood from VL patients and compared it to nested PCR. METHODS:Forty-seven subjects with clinical features (fever, hepatosplenomegaly and anemia) were confirmed positive for VL by the direct agglutination test (DAT) at titers >3200. Forty DAT negative individuals from non-endemic areas with no clinical signs or symptoms of VL served as controls. A LAMP assay was performed using a set of six primers targeting Leishmania infantum kinetoplast DNA (kDNA) minicircle gene under isothermal (64 °C) conditions. For nested PCR we used primers targeting the kDNA minicircle gene. RESULTS:The LAMP assay provided a detection limit of 1 parasite in 1 ml of peripheral blood and detected L. infantum DNA in 44 of 47 DAT-confirmed VL cases, with diagnostic sensitivity of 93.6% (95% CI). No L. infantum DNA was amplified in controls, indicating a specificity of 100%. The nested PCR yielded sensitivity of 96% (95% CI) and a specificity of 100% (95% CI). CONCLUSION:The LAMP assay gave results similar to those of nested PCR but in a shorter time. The LAMP method is simple; requires no sophisticated equipment; has a short reaction time; and results, indicated by turbidity of the reaction mixture, are observable with the naked eye.
journal_name
Iran J Parasitoljournal_title
Iranian journal of parasitologyauthors
Ghasemian M,Gharavi MJ,Akhlaghi L,Mohebali M,Meamar AR,Aryan E,Oormazdi Hsubject
Has Abstractpub_date
2014-03-01 00:00:00pages
50-9issue
1eissn
1735-7020issn
2008-238Xjournal_volume
9pub_type
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：10.18502/ijpa.v15i3.4210
更新日期：2020-07-01 00:00:00
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journal_title：Iranian journal of parasitology
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journal_title：Iranian journal of parasitology
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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更新日期：2014-09-01 00:00:00
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journal_title：Iranian journal of parasitology
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journal_title：Iranian journal of parasitology
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journal_title：Iranian journal of parasitology
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journal_title：Iranian journal of parasitology
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journal_title：Iranian journal of parasitology
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journal_title：Iranian journal of parasitology
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journal_title：Iranian journal of parasitology
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journal_title：Iranian journal of parasitology
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doi：
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
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journal_title：Iranian journal of parasitology
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doi：
更新日期：2018-04-01 00:00:00
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journal_title：Iranian journal of parasitology
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doi：
更新日期：2019-07-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2016-04-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2017-10-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2015-07-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2010-03-01 00:00:00
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journal_title：Iranian journal of parasitology
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journal_title：Iranian journal of parasitology
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2014-10-01 00:00:00
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journal_title：Iranian journal of parasitology
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更新日期：2020-04-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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更新日期：2012-01-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type：
doi：
更新日期：2016-07-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2019-10-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2014-04-01 00:00:00
