Abstract:
:Recently, we have detailed a DNA alkaline unwinding assay (DAUA) that can be used to rapidly measure chemically induced strand breaks in mammalian cells (Daniel et al., 1985). In this paper we present further development of this assay, including: (1) studies on the relationship between DNA adducts and DNA strand breaks; (2) evaluation of the role of cytotoxicity in DNA strand breaks; and (3) application of the DAUA to cell preparations from the liver of mice dosed with methylating agents. The level of DNA adducts produced in human CCRF-CEM cells by treatment with benzo(a)pyrene diol-epoxide (BPDE), N-acetoxy-2-acetyl aminofluorene (AAAF), and various methylating agents was linear with concentration over several orders of magnitude. Likewise, the level of strand breaks increased with the concentration over the same dose range. The strand breaks/adduct ratio ranged from 0.05 for the methyl adducts to 0.001 for the BPDE adducts. Using these values and the inherent sensitivity of the DAUA (circa 100 to 1000 breaks/cell), (Daniel et al., 1985), the ability of the assay to detect DNA damage induced by various classes of chemical carcinogens can be calculated. The DAUA appears to be useful for assessing the relative potency of various environmental genotoxic effects on mammalian cells. In addition, it can be conducted on cells isolated from target organs of whole animals.
journal_name
Toxicol Ind Healthjournal_title
Toxicology and industrial healthauthors
Daniel FB,Chang LW,Schenck KM,DeAngelo AB,Skelly MFdoi
10.1177/074823378900500506subject
Has Abstractpub_date
1989-10-01 00:00:00pages
647-65issue
5eissn
0748-2337issn
1477-0393journal_volume
5pub_type
杂志文章abstract::Microbiological studies were carried out on microbial contamination and antimicrobial activity of sea cucumber Holothuria polii collected from Mediterranean Sea at Abu-kir shore of Alexandria, Egypt. The obtained results revealed the presence of isolates of five human Gram-negative pathogenic bacteria, representing fi...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1177/0748233712448116
更新日期:2013-11-01 00:00:00
abstract::1,2-Dichloropropane (1,2-DCP), a solvent, which is the main component of the cleaner used in the offset printing companies in Japan, is suspected to be the causative agent of bile duct cancer, which has been recently reported at high incidence in those offset printing workplaces. While there are some reports about the...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1177/0748233714568801
更新日期:2016-09-01 00:00:00
abstract::It is well-documented that lead (Pb) toxicity can affect almost all systems in living organisms. It can induce selective autophagy of mitochondria (mitophagy) by triggering reactive oxygen species production. Emerging evidence has suggested that Pb-induced autophagy can also be activated by the endoplasmic reticulum (...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1177/0748233720971882
更新日期:2020-12-01 00:00:00
abstract::Carvacrol (CVC), a major constituent of genera Origanum and Thymus, is such a substance that has attracted attention because of its wide variety of beneficial biological activities such as antibacterial, antifungal, and anticancer effects. However, there are limited data on the cytogenetic and antioxidant effects of C...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1177/0748233713506771
更新日期:2016-04-01 00:00:00
abstract::Significant numbers of chemicals have been shown to be carcinogenic in mouse liver although they do not exhibit carcinogenic activity in other organs or tissues of mice or rats. This review focuses on the reasons for the unique susceptibility of the mouse liver to these carcinogens and the extent to which the carcinog...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1177/074823378500100421
更新日期:1985-12-01 00:00:00
abstract::Wild alligators exposed to persistent organochlorine contaminants, municipal waste compounds, and contemporary-use herbicides exhibit reproductive alterations that are thought to be caused by endocrine disruption. This study tests the hypothesis that these alterations, at least in part, result from exposure of alligat...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1191/074823399678846565
更新日期:1999-01-01 00:00:00
abstract::Pesticides are omnipresent in environment, water, fruits, and vegetables and are considered as risk factors for human health. Consumers are mainly exposed to pesticides through diet, and the main question to be answered concerns the impact of such exposure on health. In this study, we developed a mouse model to mimic ...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1177/0748233714548207
更新日期:2016-07-01 00:00:00
abstract:BACKGROUND:Hydrogen sulfide poisoning can cause severe myocardial injury, but the damage is subtle and can be easily misdiagnosed. This report presents the dynamic observation of myocardial injury associated with hydrogen sulfide poisoning. CASE REPORT:Two young men presented with symptoms of "lightning-like" death im...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1177/0748233720945184
更新日期:2020-10-01 00:00:00
abstract::The anti-genotoxic potential of hydrated sodium calcium aluminosilicate (HSCAS) was investigated using sister chromatid exchanges (SCEs) induced by aflatoxin B1 (AFB1) as genotoxic endpoint in human lymphocytes. Whole blood samples from two healthy male donors were used for this experiment and the effects of different...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1177/0748233707076738
更新日期:2007-03-01 00:00:00
abstract::In allelopathy, one plant suppresses the growth and development of other plant/plants by negatively affecting a variety of physiological and biochemical reactions. We checked the effects of methanolic extracts (allelochemical extracts) of Phytolacca latbenia (Moq.) H. Walter on antioxidant enzyme activities such as pe...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1177/0748233713483205
更新日期:2015-10-01 00:00:00
abstract::We report two cases of formaldehyde-containing hair straighteners causing new onset asthma. Formaldehyde has been reported to cause asthma in other settings. The purpose of this article is to examine two cases of asthma in hairdressers exposed to hair smoothing products and to review the relevant literature on formald...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1177/0748233717750982
更新日期:2018-04-01 00:00:00
abstract::Acrylamide has been discovered in foods cooked at high temperature. A potentially harmful effect of this dietary component has been suggested by data indicating its association with increased breast cancer. This study investigated the potential effects of acrylamide in nontumorigenic breast cells by assessing expressi...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1177/0748233710380217
更新日期:2011-02-01 00:00:00
abstract::Polycyclic aromatic hydrocarbons have mutagenic and carcinogenic properties and some of them are classified as probable or possible human carcinogens. Aim of this study was to evaluate the genotoxic risk in workers exposed to diesel exaust. Environmental and biological monitoring exposure to polycyclic aromatic hydroc...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1177/0748233707077436
更新日期:2007-02-01 00:00:00
abstract::Microcystins (MCs) are the most potent toxins that can be produced by cyanobacteria in drinking water supplies. This study investigated the abundance of toxin-producing algae in 11 drinking water treatment plants (DWTPs). A total of 26 different algal taxa were identified in treated water, from which 12% were blue gre...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1177/0748233715583203
更新日期:2016-10-01 00:00:00
abstract::The main objective of this study is to calculate the hours of diving in benzene-polluted waters acceptable for professional divers. We considered recommended absorption limit as that from pulmonary workplace absorption during an 8-h workday at recommended exposure limits set by the National Institute of Occupational S...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1177/0748233708098126
更新日期:2008-09-01 00:00:00
abstract::The purpose of this study was to describe for asthmatic subjects the distribution of individual bronchial sensitivity to sulfur dioxide (SO2). Subjects were nonsmoking male asthmatics (n = 27) who were sensitive to inhaled methacholine. None of the subjects used corticosteroids or cromolyn sodium. Oral medications wer...
journal_title:Toxicology and industrial health
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1177/074823378600200307
更新日期:1986-09-01 00:00:00
abstract::In the present study, we attempted to elucidate the induction of autophagy in rat hepatocytes by a low concentration of mercury. Hepatocytes treated with different doses of mercuric chloride (HgCl2; 1, 2.5, 5 and 10 µM) and at different time intervals (0 min, 30 min, 1 h, 2 h and 4 h) show autophagic cell death only a...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1177/0748233712462442
更新日期:2014-08-01 00:00:00
abstract::Some studies have shown increased DNA damage among car painters, but other professionals working in auto body and paint shops have not been extensively assessed. The aim of this study was to assess DNA damage in different types of auto body shop workers by measuring micronucleus (MN) levels in exfoliated buccal cells....
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1177/0748233710375948
更新日期:2010-10-01 00:00:00
abstract::Retinyl esters are the storage form of vitamin A in skin, and retinyl palmitate (RP) accounts for the majority of the retinyl esters endogenously formed in skin. RP is also obtained exogenously through the topical application of cosmetic and skin care products that contain RP. There is limited information on the penet...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1191/0748233706th253oa
更新日期:2006-05-01 00:00:00
abstract::Natural products possessing antioxidant properties play a very crucial role in ameliorating deleterious effects of reactive oxygen species. This study investigated the chemoprotective properties of methanolic extract of Vernonia amygdalina (MEVA) in an experimental model of hepatic oxidative damage induced by 2-acetyl...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1177/0748233713498436
更新日期:2016-01-01 00:00:00
abstract::The reporting of physical symptoms is influenced to a large degree by psychological processes. Individuals are more likely to notice subtle sensations in environments lacking in stimulation than those demanding external attention. The beliefs or schemas that people hold dictate where and how they attend to their bodie...
journal_title:Toxicology and industrial health
pub_type: 杂志文章,评审
doi:
更新日期:1994-07-01 00:00:00
abstract::The aim of this study was to evaluate indoor air quality and factors affecting expired carbon monoxide (CO) levels in a coffeehouse environment. This cross-sectional study was conducted at 16 randomly selected coffeehouses in Duzce, Turkey, during November 2007 to March 2008. A total of 547 people, average age 46.72 ±...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1177/0748233710383888
更新日期:2011-04-01 00:00:00
abstract:OBJECTIVE:Polycyclic aromatic hydrocarbons (PAHs) are potent environmental pollutants. Benzo[α]pyrene (B[α]P) is the major compound of PAHs that acts by activating aryl hydrocarbon receptor (AhR) in cells. B[α]P is a known carcinogen and an immunotoxicant; however, its role with regard to nuclear factor of activated T ...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1177/0748233714555153
更新日期:2016-07-01 00:00:00
abstract::The antioxidant power of the so-called antioxidants is negligible because their rate constants and concentrations are too small to compete with the specialized defense enzymes, like superoxide dismutase (SOD), for the reactive oxygen species. In this short review, we present a number of experimental data of our group,...
journal_title:Toxicology and industrial health
pub_type: 杂志文章,评审
doi:10.1177/0748233709103029
更新日期:2009-05-01 00:00:00
abstract::Using the Environmental Exposure and Sensitivity Inventory (EESI), a standardized instrument for measuring chemical sensitivity, we obtained and compared ratings of symptoms, chemical (inhalant) intolerances, other intolerances (e.g., drugs, caffeine, alcohol, skin contactants), lifeimpact, and masking (ongoing exposu...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1177/074823379901500312
更新日期:1999-04-01 00:00:00
abstract::Mercury has been used in many domains of human activities for many years, although in any form mercury is reported to be toxic. On the other hand, lichens have been used in the treatment of several diseases such as tuberculosis, hemorrhoids, ulcer, dysentery and cancer. Animal investigations on some common lichen spec...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1177/0748233711404036
更新日期:2012-02-01 00:00:00
abstract::The New York State Angler Study will evaluate the association between past and current consumption of contaminated fish from Lake Ontario and both short- and long-term health effects in a population-based cohort. It will measure fish consumption and reproductive and developmental health among 10,518 male anglers and 6...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1177/074823379601200305
更新日期:1996-05-01 00:00:00
abstract:OBJECTIVE:For several decades, there has been increasing evidence for excess incidence of lung cancer among workers in the rubber industry. The purpose of this study was to assess the risk of lung cancer occurrence among Egyptian workers involved in the rubber industry using two circulating protein biomarkers. METHODS...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1177/0748233720923504
更新日期:2020-04-01 00:00:00
abstract::Excessive exposure to nickel may cause health effects on the blood, lung, nose, kidney, reproductive system, skin and the unborn child. In the present study, we found that Ni²⁺ exposure led to a time- and dose-dependent proliferation arrest and death in human leukemia HL-60 cells. In the presence of 1 mM Ni²⁺, reactiv...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1177/0748233711433940
更新日期:2013-05-01 00:00:00
abstract::T-2 toxin is one of the mycotoxins, a group of type A trichothecenes produced by several fungal genera including Fusarium species, which may lead to the decrease of testosterone secretion in primary Leydig cells derived from mouse testis. The previous study demonstrated T-2 toxin decrease the testosterone biosynthesis...
journal_title:Toxicology and industrial health
pub_type: 杂志文章
doi:10.1177/0748233715590516
更新日期:2016-10-01 00:00:00