Abstract:
BACKGROUND:Generalized hypoxic pulmonary vasoconstriction (HPV) occurring during exposure to hypoxia is a detrimental process resulting in an increase in lung vascular resistance. Nebulization of sodium nitrite has been shown to inhibit HPV. The aim of this project was to investigate and compare the effects of nebulization of nitrite and different formulations of acidified sodium nitrite on acute HPV. METHODS:Ex vivo isolated rabbit lungs perfused with erythrocytes in Krebs-Henseleit buffer (adjusted to 10% hematocrit) and in vivo anesthetized catheterized rabbits were challenged with periods of hypoxic ventilation alternating with periods of normoxic ventilation. After baseline hypoxic challenges, vehicle, sodium nitrite or acidified sodium nitrite was delivered via nebulization. In the ex vivo model, pulmonary arterial pressure and nitric oxide concentrations in exhaled gas were monitored. Nitrite and nitrite/nitrate were measured in samples of perfusion buffer. Pulmonary arterial pressure, systemic arterial pressure, cardiac output and blood gases were monitored in the in vivo model. RESULTS:In the ex vivo model, nitrite nebulization attenuated HPV and increased nitric oxide concentrations in exhaled gas and nitrite concentrations in the perfusate. The acidified forms of sodium nitrite induced higher levels of nitric oxide in exhaled gas and had longer vasodilating effects compared to nitrite alone. All nitrite formulations increased concentrations of circulating nitrite to the same degree. In the in vivo model, inhaled nitrite inhibited HPV, while pulmonary arterial pressure, cardiac output and blood gases were not affected. All nitrite formulations had similar potency to inhibit HPV. The tested concentration of appeared tolerable. CONCLUSION:Nitrite alone and in acidified forms effectively and similarly attenuates HPV. However, acidified nitrite formulations induce a more pronounced increase in nitric oxide exhalation.
journal_name
Respir Resjournal_title
Respiratory researchauthors
Egemnazarov B,Schermuly RT,Dahal BK,Elliott GT,Hoglen NC,Surber MW,Weissmann N,Grimminger F,Seeger W,Ghofrani HAdoi
10.1186/1465-9921-11-81subject
Has Abstractpub_date
2010-06-21 00:00:00pages
81eissn
1465-9921issn
1465-993Xpii
1465-9921-11-81journal_volume
11pub_type
杂志文章abstract:BACKGROUND:Possible variation in bronchodilator response (BDR) according to age at the diagnosis of adult-onset asthma is unknown. Our aim was to assess if BDR in FEV1 is related to age at diagnosis of adult-onset asthma and how many subjects fulfill the 400 mL criterion of BDR, the suggested cut-off for asthma-like re...
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pub_type: 杂志文章
doi:10.1186/s12931-020-01441-w
更新日期:2020-07-13 00:00:00
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journal_title:Respiratory research
pub_type: 杂志文章,随机对照试验
doi:10.1186/1465-9921-7-13
更新日期:2006-01-24 00:00:00
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doi:10.1186/s12931-020-1302-9
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journal_title:Respiratory research
pub_type: 杂志文章,评审
doi:10.1186/s12931-019-1022-1
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pub_type: 杂志文章
doi:10.1186/s12931-018-0963-0
更新日期:2019-01-03 00:00:00
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journal_title:Respiratory research
pub_type: 信件
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pub_type: 杂志文章
doi:10.1186/s12931-016-0464-y
更新日期:2016-11-14 00:00:00
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journal_title:Respiratory research
pub_type: 杂志文章,多中心研究,随机对照试验
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更新日期:2017-01-11 00:00:00
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pub_type: 杂志文章,评审
doi:10.1186/rr53
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pub_type: 杂志文章
doi:10.1186/1465-9921-8-82
更新日期:2007-11-15 00:00:00
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pub_type: 杂志文章
doi:10.1186/s12931-015-0261-z
更新日期:2015-08-20 00:00:00
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pub_type: 杂志文章
doi:10.1186/1465-9921-15-29
更新日期:2014-03-12 00:00:00
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pub_type: 杂志文章
doi:10.1186/1465-9921-6-75
更新日期:2005-07-20 00:00:00
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更新日期:2017-11-22 00:00:00
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pub_type: 杂志文章
doi:10.1186/1465-9921-9-36
更新日期:2008-04-25 00:00:00
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journal_title:Respiratory research
pub_type: 杂志文章,多中心研究,随机对照试验
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pub_type: 临床试验,杂志文章,多中心研究
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pub_type: 杂志文章,多中心研究
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更新日期:2017-12-06 00:00:00
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更新日期:2019-08-23 00:00:00
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pub_type: 杂志文章
doi:10.1186/1465-9921-11-158
更新日期:2010-11-22 00:00:00
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journal_title:Respiratory research
pub_type: 杂志文章
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更新日期:2016-08-31 00:00:00
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pub_type: 杂志文章
doi:10.1186/1465-9921-10-80
更新日期:2009-08-29 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2000-01-01 00:00:00
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journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-7-6
更新日期:2006-01-12 00:00:00
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journal_title:Respiratory research
pub_type: 临床试验,杂志文章
doi:10.1186/1465-9921-13-46
更新日期:2012-06-14 00:00:00
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journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-12-157
更新日期:2011-12-16 00:00:00