Abstract:
:The attraction of leukocytes from circulation to inflamed lungs depends on the activation of both the leukocytes and the resident cells within the lung. In this study we determined gene expression and secretion patterns for monocyte chemoattractant protein-1 (MCP-1/CCL2) and T-cell specific CXCR3 agonistic chemokines (Mig/CXCL9, IP-10/CXCL10, and I-TAC/CXCL11) in TNF-alpha-, IFN-gamma-, and IL-1beta-stimulated human alveolar epithelial cells type II (AEC-II). AEC-II constitutively expressed high level of CCL2 mRNA in vitro and in situ , and released CCL2 protein in vitro . Treatment of AEC-II with proinflammatory cytokines up-regulated both CCL2 mRNA expression and release of immunoreactive CCL2, whereas IFN-gamma had no effect on CCL2 release. In contrast, CXCR3 agonistic chemokines were not detected in freshly isolated AEC-II or in non-stimulated epithelial like cell line A549. IFN-gamma, alone or in combination with IL-1beta and TNF-alpha resulted in an increase in CXCL10, CXCL11, and CXCL9 mRNA expression and generation of CXCL10 protein by AEC-II or A549 cells. CXCL10 gene expression and secretion were induced in dose-dependent manner after cytokine-stimulation of AEC-II with an order of potency IFN-gamma > > IL-1beta > or = TNF-alpha. Additionally, we localized the CCL2 and CXCL10 mRNAs in human lung tissue explants by in situ hybridization, and demonstrated the selective effects of cytokines and dexamethasone on CCL2 and CXCL10 expression. These data suggest that the regulation of the CCL2 and CXCL10 expression exhibit significant differences in their mechanisms, and also demonstrate that the alveolar epithelium contributes to the cytokine milieu of the lung, with the ability to respond to locally generated cytokines and to produce potent mediators of the local inflammatory response.
journal_name
Respir Resjournal_title
Respiratory researchauthors
Pechkovsky DV,Goldmann T,Ludwig C,Prasse A,Vollmer E,Müller-Quernheim J,Zissel Gdoi
10.1186/1465-9921-6-75keywords:
subject
Has Abstractpub_date
2005-07-20 00:00:00pages
75eissn
1465-9921issn
1465-993Xpii
1465-9921-6-75journal_volume
6pub_type
杂志文章abstract:BACKGROUND:A disintegrin and metalloproteinase domain-15 (ADAM15) is expressed by activated leukocytes, and fibroblasts in vitro. Whether ADAM15 expression is increased in the lungs of COPD patients is not known. METHODS:ADAM15 gene expression and/or protein levels were measured in whole lung and bronchoalveolar lavag...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-020-01446-5
更新日期:2020-07-16 00:00:00
abstract:BACKGROUND:Exposure to cigarette smoke is considered a major risk factor for the development of lung diseases, since its causative role has been assessed in the induction and maintenance of an inflamed state in the airways. Lung fibroblasts can contribute to these processes, due to their ability to produce proinflammat...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-8-23
更新日期:2007-03-12 00:00:00
abstract:BACKGROUND:ATP-regulated potassium channels (KATP) regulate pulmonary vascular tone and are involved in hypoxic pulmonary vasoconstriction (HPV). In patients with inflammation like sepsis or ARDS, HPV is impaired, resulting in a ventilation-perfusion mismatch and hypoxia. Since increase of vascular KATP channel Kir6.1 ...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-018-0735-x
更新日期:2018-02-13 00:00:00
abstract:BACKGROUND:Motor vehicle-related air pollution can potentially impair lung function. The effect of pollution in people with compromised pulmonary function such as in COPD has not been previously investigated. To examine the association of lung function with motor vehicle density in people with spirometrically determine...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-016-0451-3
更新日期:2016-10-24 00:00:00
abstract::Asthmatics are more susceptible to influenza infections, yet mechanisms mediating this enhanced susceptibility are unknown. Influenza virus hemagglutinin (HA) protein binds to sialic acid residues on the host cells. HA requires cleavage to allow fusion of the viral HA with host cell membrane, which is mediated by host...
journal_title:Respiratory research
pub_type: 信件
doi:10.1186/1465-9921-13-82
更新日期:2012-09-19 00:00:00
abstract:BACKGROUND:Little is known about the association between cigarette smoking and asthma severity. We assessed smoking as a determinant of disease severity and control in a cohort of clinic-referred allergic subjects who developed new onset asthma. METHODS:Allergic rhinitis subjects with no asthma (n = 371) were followed...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-12-16
更新日期:2011-01-24 00:00:00
abstract:BACKGROUND:The development of bronchial hyperreactivity (BHR) subsequent to precapillary pulmonary hypertension (PHT) was prevented by acting on the major signalling pathways (endothelin, nitric oxide, vasoactive intestine peptide (VIP) and prostacyclin) involved in the control of the pulmonary vascular and bronchial t...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-12-58
更新日期:2011-04-27 00:00:00
abstract:BACKGROUND:Exacerbations of chronic obstructive pulmonary disease (COPD) are associated with loss of lung function and poor outcomes for patients. However, there are limited data on the time course of changes in forced expiratory volume in 1 s (FEV1) preceding the first reported symptom and after the start of an exacer...
journal_title:Respiratory research
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1186/s12931-018-0944-3
更新日期:2018-12-13 00:00:00
abstract:BACKGROUND:The assessment of bronchodilator-induced change in forced vital capacity (FVC) is dependent on forced expiratory time (FET) in subjects with airflow limitation. Limited information is available on the concurrent responses of FVC, forced expiratory volume in six seconds (FEV6), and FET in the bronchodilation ...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-10-71
更新日期:2009-07-28 00:00:00
abstract:BACKGROUND:Inhaled corticosteroids are the recommended first-line treatment for asthma but adherence to therapy is suboptimal. The objectives of this study were to compare the efficacy and safety of once-daily (OD) evening and twice-daily (BD) regimens of the novel inhaled corticosteroid fluticasone furoate (FF) in ast...
journal_title:Respiratory research
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1186/1465-9921-12-160
更新日期:2011-12-21 00:00:00
abstract:BACKGROUND:There are limited series concerning Dieulafoy disease of the bronchus. We describe the clinical presentation of a series of 7 patients diagnosed with Dieulafoy disease of the bronchus and provide information about the pathological diagnosis approach. PATIENTS AND METHODS:A retrospective review of patients w...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-9-58
更新日期:2008-08-05 00:00:00
abstract:BACKGROUND:Pulmonary sarcoidosis is an inflammatory disease, characterized by an accumulation of CD4+ lymphocytes and the formation of non-caseating epithelioid cell granulomas in the lungs. The disease either resolves spontaneously or develops into a chronic disease with fibrosis. The neurotrophins nerve growth factor...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-11-156
更新日期:2010-11-08 00:00:00
abstract:BACKGROUND:Generalized hypoxic pulmonary vasoconstriction (HPV) occurring during exposure to hypoxia is a detrimental process resulting in an increase in lung vascular resistance. Nebulization of sodium nitrite has been shown to inhibit HPV. The aim of this project was to investigate and compare the effects of nebuliza...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-11-81
更新日期:2010-06-21 00:00:00
abstract::The molecular mechanisms of Idiopathic Pulmonary Fibrosis (IPF) remain elusive. Transforming Growth Factor beta 1(TGF-beta1) is a key effector cytokine in the development of lung fibrosis. We used microarray and computational biology strategies to identify genes whose expression is significantly altered in alveolar ep...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-7-114
更新日期:2006-09-01 00:00:00
abstract:BACKGROUND:Radiation pneumonitis (RP) is a frequent complication of concurrent chemoradiotherapy (CCRT) and is associated with severe symptoms that decrease quality of life and might result in pulmonary fibrosis or death. The aim of this study is to identify whether pulmonary function test (PFT) abnormalities may predi...
journal_title:Respiratory research
pub_type: 杂志文章,多中心研究
doi:10.1186/s12931-018-0775-2
更新日期:2018-04-24 00:00:00
abstract:BACKGROUND:Airway smooth muscle contraction is critical for maintenance of appropriate airway tone, and has been implicated in asthma pathogenesis. Smooth muscle contraction requires an "engine" (myosin activation) and a "transmission system" (actin cytoskeletal remodeling). However, the mechanisms that control actin r...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-017-0709-4
更新日期:2018-01-05 00:00:00
abstract::Transposable elements (TEs) are a class of mobile genetic elements (MGEs) that were long regarded as junk DNA, which make up approximately 45% of the genome. Although most of these elements are rendered inactive by mutations and other gene silencing mechanisms, TEs such as long interspersed nuclear elements (LINEs) ar...
journal_title:Respiratory research
pub_type: 杂志文章,评审
doi:10.1186/1465-9921-14-99
更新日期:2013-10-05 00:00:00
abstract:BACKGROUND:The home is the predominant environment for exposure to many environmental irritants such as air pollutants and allergens. Exposure to common indoor irritants including volatile organic compounds, formaldehyde and nitrogen dioxide, may increase the risk of snoring for children. The aim of this study was to i...
journal_title:Respiratory research
pub_type: 杂志文章,随机对照试验
doi:10.1186/1465-9921-5-19
更新日期:2004-11-04 00:00:00
abstract:BACKGROUND:Pulmonary neuroendocrine cells (PNEC) are specialized epithelial cells that are thought to play important roles in lung development and airway function. PNEC occur either singly or in clusters called neuroepithelial bodies. Our aim was to characterize the three dimensional morphology of PNEC, their distribut...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-6-115
更新日期:2005-10-10 00:00:00
abstract:: ...
journal_title:Respiratory research
pub_type: 社论
doi:10.1186/s12931-018-0929-2
更新日期:2018-11-26 00:00:00
abstract::The novel coronavirus disease (COVID-19) outbreak started in December 2019 in Wuhan, China, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The CT image is used to assess the disease progress, whereas the continued two times of negative results from SARS-CoV-2 nucleic acid detection had been co...
journal_title:Respiratory research
pub_type: 信件
doi:10.1186/s12931-020-01363-7
更新日期:2020-04-22 00:00:00
abstract::Respiratory diseases including asthma and chronic obstructive pulmonary disease (COPD) are characterised by excessive and persistent inflammation. Current treatments are often inadequate for symptom and disease control, and hence new therapies are warranted. Recent emerging research has implicated dyslipidaemia in pul...
journal_title:Respiratory research
pub_type: 杂志文章,评审
doi:10.1186/s12931-017-0526-9
更新日期:2017-02-28 00:00:00
abstract:BACKGROUND:Idiopathic pulmonary fibrosis (IPF) is a chronically progressive interstitial lung disease of unknown etiology. Previously, we have demonstrated the selective upregulation of the macrophage-derived chemokine CCL22 and the thymus activation-regulated chemokine CCL17 among chemokines, in a rat model of radiati...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-10-80
更新日期:2009-08-29 00:00:00
abstract:BACKGROUND:Real-world evidence (RWE) can inform patient management decisions, but RWE studies are associated with limitations. Linkage of different RWE data types could address such limitations by enriching data and improving scientific quality. Using the example of chronic obstructive pulmonary disease (COPD) in Germa...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-018-0865-1
更新日期:2018-08-30 00:00:00
abstract:BACKGROUND:Recent research has suggested that the Th1 and Th2 chemokine/cytokine axis contributes to the development of chronic hypersensitivity pneumonitis (HP). Acute exacerbations (AE) are significant factors in the prognosis of chronic HP. Little is known, however, about these biomarkers in association with AE in c...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-14-57
更新日期:2013-05-25 00:00:00
abstract:BACKGROUND:An important goal of chronic obstructive pulmonary disease (COPD) treatment is to reduce the frequency of exacerbations. Some observations suggest a decline in exacerbation rates in clinical trials over time. A more systematic understanding would help to improve the design and interpretation of COPD trials. ...
journal_title:Respiratory research
pub_type: 杂志文章,meta分析
doi:10.1186/s12931-019-1163-2
更新日期:2019-08-16 00:00:00
abstract:BACKGROUND:Chronic respiratory diseases (CRDs) are leading causes of morbidity worldwide. However, the spatial and temporal trends in prevalence and incidence of CRDs have not been estimated. METHODS:Based on data from the Global Burden of Diseases, Injuries, and Risk Factors Study 2017, we analyzed the prevalence and...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-020-1291-8
更新日期:2020-02-11 00:00:00
abstract:BACKGROUND:The relationship between the Epworth sleepiness scale (ESS) and the apnea-hypopnea index (AHI) is uncertain and even poor. The major problem associated with the ESS might be a lack of consideration of weight in prediction in clinical practice. Would awarding different item-scores to the four scales of ESS it...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-020-01417-w
更新日期:2020-06-12 00:00:00
abstract:BACKGROUND:While glucocorticoids are currently the most effective therapy for asthma, associated side effects limit enthusiasm for their use. Peroxisome proliferator-activated receptor-gamma (PPAR-gamma) activators include the synthetic thiazolidinediones (TZDs) which exhibit anti-inflammatory effects that suggest usef...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-8-90
更新日期:2007-12-04 00:00:00
abstract::Monitoring of pulmonary physiology is fundamental to the clinical management of patients with Cystic Fibrosis. The current standard clinical practise uses spirometry to assess lung function which delivers a clinically relevant functional readout of total lung function, however does not supply any visible or localised ...
journal_title:Respiratory research
pub_type: 杂志文章,评审
doi:10.1186/s12931-017-0578-x
更新日期:2017-05-17 00:00:00