CCR2 and CXCR3 agonistic chemokines are differently expressed and regulated in human alveolar epithelial cells type II.


:The attraction of leukocytes from circulation to inflamed lungs depends on the activation of both the leukocytes and the resident cells within the lung. In this study we determined gene expression and secretion patterns for monocyte chemoattractant protein-1 (MCP-1/CCL2) and T-cell specific CXCR3 agonistic chemokines (Mig/CXCL9, IP-10/CXCL10, and I-TAC/CXCL11) in TNF-alpha-, IFN-gamma-, and IL-1beta-stimulated human alveolar epithelial cells type II (AEC-II). AEC-II constitutively expressed high level of CCL2 mRNA in vitro and in situ , and released CCL2 protein in vitro . Treatment of AEC-II with proinflammatory cytokines up-regulated both CCL2 mRNA expression and release of immunoreactive CCL2, whereas IFN-gamma had no effect on CCL2 release. In contrast, CXCR3 agonistic chemokines were not detected in freshly isolated AEC-II or in non-stimulated epithelial like cell line A549. IFN-gamma, alone or in combination with IL-1beta and TNF-alpha resulted in an increase in CXCL10, CXCL11, and CXCL9 mRNA expression and generation of CXCL10 protein by AEC-II or A549 cells. CXCL10 gene expression and secretion were induced in dose-dependent manner after cytokine-stimulation of AEC-II with an order of potency IFN-gamma > > IL-1beta > or = TNF-alpha. Additionally, we localized the CCL2 and CXCL10 mRNAs in human lung tissue explants by in situ hybridization, and demonstrated the selective effects of cytokines and dexamethasone on CCL2 and CXCL10 expression. These data suggest that the regulation of the CCL2 and CXCL10 expression exhibit significant differences in their mechanisms, and also demonstrate that the alveolar epithelium contributes to the cytokine milieu of the lung, with the ability to respond to locally generated cytokines and to produce potent mediators of the local inflammatory response.


Respir Res


Respiratory research


Pechkovsky DV,Goldmann T,Ludwig C,Prasse A,Vollmer E,Müller-Quernheim J,Zissel G





Has Abstract


2005-07-20 00:00:00












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    abstract:BACKGROUND:Generalized hypoxic pulmonary vasoconstriction (HPV) occurring during exposure to hypoxia is a detrimental process resulting in an increase in lung vascular resistance. Nebulization of sodium nitrite has been shown to inhibit HPV. The aim of this project was to investigate and compare the effects of nebuliza...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Egemnazarov B,Schermuly RT,Dahal BK,Elliott GT,Hoglen NC,Surber MW,Weissmann N,Grimminger F,Seeger W,Ghofrani HA

    更新日期:2010-06-21 00:00:00

  • IL-33, diet-induced obesity, and pulmonary responses to ozone.

    abstract:BACKGROUND:Obesity augments pulmonary responses to ozone. We have reported that IL-33 contributes to these effects of obesity in db/db mice. The purpose of this study was to determine whether IL-33 also contributes to obesity-related changes in the response to ozone in mice with diet-induced obesity. METHODS:Male wild...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Kasahara DI,Shore SA

    更新日期:2020-04-23 00:00:00

  • Ovalbumin sensitization and challenge increases the number of lung cells possessing a mesenchymal stromal cell phenotype.

    abstract:BACKGROUND:Recent studies have indicated the presence of multipotent mesenchymal stromal cells (MSCs) in human lung diseases. Excess airway smooth muscle, myofibroblasts and activated fibroblasts have each been noted in asthma, suggesting that mesenchymal progenitor cells play a role in asthma pathogenesis. We therefor...

    journal_title:Respiratory research

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    authors: Bentley JK,Popova AP,Bozyk PD,Linn MJ,Baek AE,Lei J,Goldsmith AM,Hershenson MB

    更新日期:2010-09-21 00:00:00

  • Relation of exaggerated cytokine responses of CF airway epithelial cells to PAO1 adherence.

    abstract::In many model systems, cystic fibrosis (CF) phenotype airway epithelial cells in culture respond to P. aeruginosa with greater interleukin (IL)-8 and IL-6 secretion than matched controls. In order to test whether this excess inflammatory response results from the reported increased adherence of P. aeruginosa to the CF...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Kube DM,Fletcher D,Davis PB

    更新日期:2005-07-11 00:00:00

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    abstract::MMP28 is constitutively expressed by epithelial cells in many tissues, including the respiratory epithelium in the lung and keratinocytes in the skin. This constitutive expression suggests that MMP28 may serve a role in epithelial cell homeostasis. In an effort to determine its function in epithelial cell biology, we ...

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    authors: Manicone AM,Harju-Baker S,Johnston LK,Chen AJ,Parks WC

    更新日期:2011-10-31 00:00:00

  • A retinoid X receptor partial agonist attenuates pulmonary emphysema and airway inflammation.

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    authors: Morichika D,Miyahara N,Fujii U,Taniguchi A,Oda N,Senoo S,Kataoka M,Tanimoto M,Kakuta H,Kiura K,Maeda Y,Kanehiro A

    更新日期:2019-01-03 00:00:00

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    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Calabrese F,Giacometti C,Beghe B,Rea F,Loy M,Zuin R,Marulli G,Baraldo S,Saetta M,Valente M

    更新日期:2005-02-10 00:00:00

  • Role of TNF-alpha in lung tight junction alteration in mouse model of acute lung inflammation.

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    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Mazzon E,Cuzzocrea S

    更新日期:2007-10-30 00:00:00

  • Oxygen-regulated transcription factors and their role in pulmonary disease.

    abstract::The transcription factors nuclear factor interleukin-6 (NF-IL6), early growth response-1 (EGR-1) and hypoxia-inducible factor-1 (HIF-1) have important roles in the molecular pathophysiology of hypoxia-associated pulmonary disease. NF-IL6 controls the production of interleukin (IL)-6 in vascular endothelial cells, whic...

    journal_title:Respiratory research

    pub_type: 杂志文章,评审


    authors: Semenza GL

    更新日期:2000-01-01 00:00:00

  • Progression of pulmonary hyperinflation and trapped gas associated with genetic and environmental factors in children with cystic fibrosis.

    abstract:BACKGROUND:Functional deterioration in cystic fibrosis (CF) may be reflected by increasing bronchial obstruction and, as recently shown, by ventilation inhomogeneities. This study investigated which physiological factors (airway obstruction, ventilation inhomogeneities, pulmonary hyperinflation, development of trapped ...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Kraemer R,Baldwin DN,Ammann RA,Frey U,Gallati S

    更新日期:2006-11-30 00:00:00

  • Exogenous interleukin-6, interleukin-13, and interferon-γ provoke pulmonary abnormality with mild edema in enterovirus 71-infected mice.

    abstract:BACKGROUND:Neonatal mice developed neurological disease and pulmonary dysfunction after an infection with a mouse-adapted human Enterovirus 71 (EV71) strain MP4. However, the hallmark of severe human EV71 infection, pulmonary edema (PE), was not evident. METHODS:To test whether EV71-induced PE required a proinflammato...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Huang SW,Lee YP,Hung YT,Lin CH,Chuang JI,Lei HY,Su IJ,Yu CK

    更新日期:2011-11-06 00:00:00

  • Rate of progression of CT-quantified emphysema in male current and ex-smokers: a follow-up study.

    abstract:BACKGROUND:Little is known about the factors associated with CT-quantified emphysema progression in heavy smokers. The objective of this study was to investigate the effect of length of smoking cessation and clinical / demographical factors on the rate of emphysema progression and FEV1-decline in male heavy smokers. M...

    journal_title:Respiratory research

    pub_type: 杂志文章,随机对照试验


    authors: Mohamed Hoesein FA,Zanen P,de Jong PA,van Ginneken B,Boezen HM,Groen HJ,Oudkerk M,de Koning HJ,Postma DS,Lammers JW

    更新日期:2013-05-20 00:00:00

  • Auxiliary diagnostic value of tumor biomarkers in pleural fluid for lung cancer-associated malignant pleural effusion.

    abstract:BACKGROUND:Pleural effusion (PE) can be divided into benign pleural effusion (BPE) and malignant pleural effusion (MPE). There is no consensus on the identification of lung cancer-associated MPE using the optimal cut-off levels from five common tumor biomarkers (CEA, CYFRA 21-1, CA125, SCC-Ag, and NSE). Therefore, we a...

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    pub_type: 杂志文章


    authors: Zhang H,Li C,Hu F,Zhang X,Shen Y,Chen Y,Li F

    更新日期:2020-10-29 00:00:00

  • Association of mast cells with lung function in chronic obstructive pulmonary disease.

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    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Gosman MM,Postma DS,Vonk JM,Rutgers B,Lodewijk M,Smith M,Luinge MA,Ten Hacken NH,Timens W

    更新日期:2008-09-10 00:00:00

  • Lung function imaging methods in Cystic Fibrosis pulmonary disease.

    abstract::Monitoring of pulmonary physiology is fundamental to the clinical management of patients with Cystic Fibrosis. The current standard clinical practise uses spirometry to assess lung function which delivers a clinically relevant functional readout of total lung function, however does not supply any visible or localised ...

    journal_title:Respiratory research

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    authors: Kołodziej M,de Veer MJ,Cholewa M,Egan GF,Thompson BR

    更新日期:2017-05-17 00:00:00

  • Lung function reductions associated with motor vehicle density in chronic obstructive pulmonary disease: a cross-sectional study.

    abstract:BACKGROUND:Motor vehicle-related air pollution can potentially impair lung function. The effect of pollution in people with compromised pulmonary function such as in COPD has not been previously investigated. To examine the association of lung function with motor vehicle density in people with spirometrically determine...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Nitschke M,Appleton SL,Li Q,Tucker GR,Shah P,Bi P,Pisaniello DL,Adams RJ

    更新日期:2016-10-24 00:00:00

  • Pectoralis muscle area and mortality in smokers without airflow obstruction.

    abstract:BACKGROUND:Low muscle mass is associated with increased mortality in the general population but its prognostic value in at-risk smokers, those without expiratory airflow obstruction, is unknown. We aimed to test the hypothesis that reduced muscle mass is associated with increased mortality in at-risk smokers. METHODS:...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Diaz AA,Martinez CH,Harmouche R,Young TP,McDonald ML,Ross JC,Han ML,Bowler R,Make B,Regan EA,Silverman EK,Crapo J,Boriek AM,Kinney GL,Hokanson JE,Estepar RSJ,Washko GR

    更新日期:2018-04-10 00:00:00

  • Health care resource utilization and cost for asthma patients regularly treated with oral corticosteroids - a Swedish observational cohort study (PACEHR).

    abstract:BACKGROUND:Patients with severe uncontrolled asthma may receive oral corticosteroid (OCS) treatment regularly. The present study investigated the health care resource utilization and cost in regularly OCS treated Swedish asthma patients. METHODS:Primary care medical records data were linked to data from Swedish nation...

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    pub_type: 杂志文章


    authors: Janson C,Lisspers K,Ställberg B,Johansson G,Telg G,Thuresson M,Nordahl Christensen H,Larsson K

    更新日期:2018-09-03 00:00:00

  • Neurotrophins and neurotrophin receptors in pulmonary sarcoidosis - granulomas as a source of expression.

    abstract:BACKGROUND:Pulmonary sarcoidosis is an inflammatory disease, characterized by an accumulation of CD4+ lymphocytes and the formation of non-caseating epithelioid cell granulomas in the lungs. The disease either resolves spontaneously or develops into a chronic disease with fibrosis. The neurotrophins nerve growth factor...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Dagnell C,Grunewald J,Kramar M,Haugom-Olsen H,Elmberger GP,Eklund A,Olgart Höglund C

    更新日期:2010-11-08 00:00:00

  • Childhood infections and asthma: at the crossroads of the hygiene and Barker hypotheses.

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    journal_title:Respiratory research

    pub_type: 杂志文章,评审


    authors: Tantisira KG,Weiss ST

    更新日期:2001-01-01 00:00:00

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    abstract:BACKGROUND:Cigarette smoke is a major risk factor for chronic obstructive pulmonary disease (COPD), an inflammatory lung disorder. COPD is characterized by an increase in CD8(+) T cells within the central and peripheral airways. We hypothesized that the CD8(+) T cells in COPD patients have increased Toll-like receptor ...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Nadigel J,Préfontaine D,Baglole CJ,Maltais F,Bourbeau J,Eidelman DH,Hamid Q

    更新日期:2011-11-09 00:00:00

  • Cleaved cytokeratin-18 is a mechanistically informative biomarker in idiopathic pulmonary fibrosis.

    abstract:BACKGROUND:Stress of the endoplasmic reticulum (ER) leading to activation of the unfolded protein response (UPR) and alveolar epithelial cell (AEC) apoptosis may play a role in the pathogenesis of idiopathic pulmonary fibrosis (IPF). Our objectives were to determine whether circulating caspase-cleaved cytokeratin-18 (c...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Cha SI,Ryerson CJ,Lee JS,Kukreja J,Barry SS,Jones KD,Elicker BM,Kim DS,Papa FR,Collard HR,Wolters PJ

    更新日期:2012-11-20 00:00:00

  • Laminin-332 alters connexin profile, dye coupling and intercellular Ca2+ waves in ciliated tracheal epithelial cells.

    abstract:BACKGROUND:Tracheal epithelial cells are anchored to a dynamic basement membrane that contains a variety of extracellular matrix proteins including collagens and laminins. During development, wound repair and disease of the airway epithelium, significant changes in extracellular matrix proteins may directly affect cell...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Isakson BE,Olsen CE,Boitano S

    更新日期:2006-08-02 00:00:00

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    abstract:BACKGROUND:Airway epithelial barrier function is maintained by the formation of tight junctions (TJs) and adherens junctions (AJs). Inhalation of cigarette smoke causes airway epithelial barrier dysfunction and may contribute to the pathogenesis of chronic lung diseases such as asthma and chronic obstructive pulmonary ...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Tatsuta M,Kan-O K,Ishii Y,Yamamoto N,Ogawa T,Fukuyama S,Ogawa A,Fujita A,Nakanishi Y,Matsumoto K

    更新日期:2019-11-09 00:00:00

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    abstract:BACKGROUND:Low body mass index has been associated with increased mortality in severe COPD. The impact of body composition earlier in the disease remains unclear. We studied the impact of body composition on the risk of functional limitation in COPD. METHODS:We used bioelectrical impedance to estimate body composition...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Eisner MD,Blanc PD,Sidney S,Yelin EH,Lathon PV,Katz PP,Tolstykh I,Ackerson L,Iribarren C

    更新日期:2007-01-29 00:00:00

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    abstract:BACKGROUND:Early life impairments leading to lower lung function by adulthood are considered as risk factors for chronic obstructive pulmonary disease (COPD). Recently, we compared the lung transcriptomic profile between two mouse strains with extreme total lung capacities to identify plausible pulmonary function deter...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Vishweswaraiah S,George L,Purushothaman N,Ganguly K

    更新日期:2018-06-06 00:00:00

  • Microarray identifies ADAM family members as key responders to TGF-beta1 in alveolar epithelial cells.

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    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Keating DT,Sadlier DM,Patricelli A,Smith SM,Walls D,Egan JJ,Doran PP

    更新日期:2006-09-01 00:00:00

  • Chitinase-like protein YKL-40 correlates with inflammatory phenotypes, anti-asthma responsiveness and future exacerbations.

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    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Liu L,Zhang X,Liu Y,Zhang L,Zheng J,Wang J,Hansbro PM,Wang L,Wang G,Hsu AC

    更新日期:2019-05-22 00:00:00

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    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Guthmann F,Wissel H,Schachtrup C,Tölle A,Rüdiger M,Spener F,Rüstow B

    更新日期:2005-01-21 00:00:00

  • LPS induces IL-10 production by human alveolar macrophages via MAPKinases- and Sp1-dependent mechanisms.

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    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Chanteux H,Guisset AC,Pilette C,Sibille Y

    更新日期:2007-10-04 00:00:00