A confocal microscopic study of solitary pulmonary neuroendocrine cells in human airway epithelium.


BACKGROUND:Pulmonary neuroendocrine cells (PNEC) are specialized epithelial cells that are thought to play important roles in lung development and airway function. PNEC occur either singly or in clusters called neuroepithelial bodies. Our aim was to characterize the three dimensional morphology of PNEC, their distribution, and their relationship to the epithelial nerves in whole mounts of adult human bronchi using confocal microscopy. METHODS:Bronchi were resected from non-diseased portions of a lobe of human lung obtained from 8 thoracotomy patients (Table 1) undergoing surgery for the removal of lung tumors. Whole mounts were stained with antibodies to reveal all nerves (PGP 9.5), sensory nerves (calcitonin gene related peptide, CGRP), and PNEC (PGP 9.5, CGRP and gastrin releasing peptide, GRP). The analysis and rendition of the resulting three-dimensional data sets, including side-projections, was performed using NIH-Image software. Images were colorized and super-imposed using Adobe Photoshop. RESULTS:PNEC were abundant but not homogenously distributed within the epithelium, with densities ranging from 65/mm2 to denser patches of 250/mm2, depending on the individual wholemount. Rotation of 3-D images revealed a complex morphology; flask-like with the cell body near the basement membrane and a thick stem extending to the lumen. Long processes issued laterally from its base, some lumenal and others with feet-like processes. Calcitonin gene-related peptide (CGRP) was present in about 20% of PNEC, mainly in the processes. CGRP-positive nerves were sparse, with some associated with the apical part of the PNEC. CONCLUSION:Our 3D-data demonstrates that PNEC are numerous and exhibit a heterogeneous peptide content suggesting an active and diverse PNEC population.


Respir Res


Respiratory research


Weichselbaum M,Sparrow MP,Hamilton EJ,Thompson PJ,Knight DA





Has Abstract


2005-10-10 00:00:00












  • Prevalence and incidence of sarcoidosis in Korea: a nationwide population-based study.

    abstract:BACKGROUND:The prevalence and incidence of sarcoidosis varies worldwide. We estimated the prevalence and incidence of sarcoidosis in Korea using nationwide claims data from the Korean Health Insurance Review and Assessment Service. METHODS:Cases of sarcoidosis were identified for any visit between 2007 to 2016 that li...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Yoon HY,Kim HM,Kim YJ,Song JW

    更新日期:2018-08-28 00:00:00

  • TLR4 signaling is essential for survival in acute lung injury induced by virulent Pseudomonas aeruginosa secreting type III secretory toxins.

    abstract:BACKGROUND:The relative contributions of the cytotoxic phenotype of P. aeruginosa expressing type III secretory toxins and an immunocompromised condition lacking normal Toll-like receptor 4 (TLR4) signaling in the pathogenesis of acute lung injury and sepsis were evaluated in a mouse model for Pseudomonas aeruginosa pn...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Faure K,Sawa T,Ajayi T,Fujimoto J,Moriyama K,Shime N,Wiener-Kronish JP

    更新日期:2004-02-12 00:00:00

  • Determination of rheology and surface tension of airway surface liquid: a review of clinical relevance and measurement techniques.

    abstract::By airway surface liquid, we mean a thin fluid continuum consisting of the airway lining layer and the alveolar lining layer, which not only serves as a protective barrier against foreign particles but also contributes to maintaining normal respiratory mechanics. In recent years, measurements of the rheological proper...

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    pub_type: 杂志文章,评审


    authors: Chen Z,Zhong M,Luo Y,Deng L,Hu Z,Song Y

    更新日期:2019-12-04 00:00:00

  • Phenotyping community-acquired pneumonia according to the presence of acute respiratory failure and severe sepsis.

    abstract:BACKGROUND:Acute respiratory failure (ARF) and severe sepsis (SS) are possible complications in patients with community-acquired pneumonia (CAP). The aim of the study was to evaluate prevalence, characteristics, risk factors and impact on mortality of hospitalized patients with CAP according to the presence of ARF and ...

    journal_title:Respiratory research

    pub_type: 杂志文章,多中心研究


    authors: Aliberti S,Brambilla AM,Chalmers JD,Cilloniz C,Ramirez J,Bignamini A,Prina E,Polverino E,Tarsia P,Pesci A,Torres A,Blasi F,Cosentini R

    更新日期:2014-03-04 00:00:00

  • Use of Berlin questionnaire in comparison to polysomnography and home sleep study in patients with obstructive sleep apnea.

    abstract:BACKGROUND:Obstructive sleep apnea syndrome (OSAS) is a common disorder with significant morbidity and mortality. We aimed to evaluate the predictive accuracy of the Berlin questionnaire in patients with suspected OSAS undergoing PSG in the sleep laboratory setting against those going through the Embletta™ portable dia...

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    pub_type: 杂志文章,随机对照试验


    authors: Ng SS,Tam W,Chan TO,To KW,Ngai J,Chan KKP,Yip WH,Lo RL,Yiu K,Ko FW,Hui DS

    更新日期:2019-02-22 00:00:00

  • Intermittent hypoxia promotes melanoma lung metastasis via oxidative stress and inflammation responses in a mouse model of obstructive sleep apnea.

    abstract:BACKGROUND:Recently, increased tumor incidence and cancer-related mortality have been reported among patients with obstructive sleep apnea (OSA). Intermittent hypoxia (IH), the hallmark feature of OSA, contributes to the metastasis of tumors. However, the molecular mechanisms by which tumor metastasis is accelerated by...

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    authors: Li L,Ren F,Qi C,Xu L,Fang Y,Liang M,Feng J,Chen B,Ning W,Cao J

    更新日期:2018-02-12 00:00:00

  • Altered molecular specificity of surfactant phosphatidycholine synthesis in patients with acute respiratory distress syndrome.

    abstract:BACKGROUND:Acute respiratory distress syndrome (ARDS) is a life-threatening critical illness, characterised by qualitative and quantitative surfactant compositional changes associated with premature airway collapse, gas-exchange abnormalities and acute hypoxic respiratory failure. The underlying mechanisms for this dys...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Dushianthan A,Goss V,Cusack R,Grocott MP,Postle AD

    更新日期:2014-11-07 00:00:00

  • Gene susceptibility identification in a longitudinal study confirms new loci in the development of chronic obstructive pulmonary disease and influences lung function decline.

    abstract:BACKGROUND:To identify COPD associated gene susceptibility and lung function in a longitudinal cohort including COPD and subjects who were at risk for developing COPD, and to replicate this in two cross-sectional and longitudinal populations in Chinese Han population. METHODS:Three cohorts were recruited in this study...

    journal_title:Respiratory research

    pub_type: 杂志文章,多中心研究


    authors: Xie J,Wu H,Xu Y,Wu X,Liu X,Shang J,Zhao J,Zhao J,Wang J,Dela Cruz CS,Xiong W,Xu Y

    更新日期:2015-04-18 00:00:00

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    pub_type: 杂志文章


    authors: Miniati M,Monti S,Basta G,Cocci F,Fornai E,Bottai M

    更新日期:2011-03-30 00:00:00

  • Quantitative CT analysis using functional imaging is superior in describing disease progression in idiopathic pulmonary fibrosis compared to forced vital capacity.

    abstract:BACKGROUND:Idiopathic pulmonary fibrosis (IPF) is chronic fibrosing pneumonia with an unpredictable natural disease history. Functional respiratory imaging (FRI) has potential to better characterize this disease. The aim of this study was to identify FRI parameters, which predict FVC decline in patients with IPF. METH...

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    pub_type: 临床试验,杂志文章


    authors: Clukers J,Lanclus M,Mignot B,Van Holsbeke C,Roseman J,Porter S,Gorina E,Kouchakji E,Lipson KE,De Backer W,De Backer J

    更新日期:2018-11-06 00:00:00

  • LPS induces IL-10 production by human alveolar macrophages via MAPKinases- and Sp1-dependent mechanisms.

    abstract:BACKGROUND:IL-10 is a cytokine mainly produced by macrophages that plays key roles in tolerance to inhaled antigens and in lung homeostasis. Its regulation in alveolar macrophages (HAM), the resident lung phagocytes, remains however unknown. METHODS:The present study investigated the role of intracellular signalling a...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Chanteux H,Guisset AC,Pilette C,Sibille Y

    更新日期:2007-10-04 00:00:00

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    abstract:BACKGROUND:Family history of asthma and other allergic diseases have been linked to the risk of childhood asthma previously, but little is known about their effect on the age-of-onset and persistency of asthma until young adulthood. METHODS:We assessed the effect of the family history of asthma and allergic diseases o...

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    authors: Paaso EM,Jaakkola MS,Rantala AK,Hugg TT,Jaakkola JJ

    更新日期:2014-11-27 00:00:00

  • Hospitalisation and mortality in patients with comorbid COPD and heart failure: a systematic review and meta-analysis.

    abstract:BACKGROUND:Discrepancy exists amongst studies investigating the effect of comorbid heart failure (HF) on the morbidity and mortality of chronic obstructive pulmonary disease (COPD) patients. METHODS:MEDLINE and Embase were searched using a pre-specified search strategy for studies comparing hospitalisation, rehospital...

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    authors: Axson EL,Ragutheeswaran K,Sundaram V,Bloom CI,Bottle A,Cowie MR,Quint JK

    更新日期:2020-02-14 00:00:00

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    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Kainu A,Lindqvist A,Sarna S,Lundbäck B,Sovijärvi A

    更新日期:2009-07-28 00:00:00

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    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Fan XY,van den Berg A,Snoek M,van der Flier LG,Smids B,Jansen HM,Liu RY,Lutter R

    更新日期:2009-07-03 00:00:00

  • Exchange protein directly activated by cAMP (Epac) protects against airway inflammation and airway remodeling in asthmatic mice.

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    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Chen YF,Huang G,Wang YM,Cheng M,Zhu FF,Zhong JN,Gao YD

    更新日期:2019-12-18 00:00:00

  • Viruses, dendritic cells and the lung.

    abstract::The interaction between viruses and dendritic cells (DCs) is varied and complex. DCs are key elements in the development of a host response to pathogens such as viruses, but viruses have developed survival tactics to either evade or diminish the immune system that functions to kill and eliminate these micro-organisms....

    journal_title:Respiratory research

    pub_type: 杂志文章,评审


    authors: Peebles RS Jr,Graham BS

    更新日期:2001-01-01 00:00:00

  • Diagnostic value of plasma and bronchoalveolar lavage samples in acute lung allograft rejection: differential cytology.

    abstract::Diagnosis of acute lung allograft rejection is currently based on transbronchial lung biopsies. Additional methods to detect acute allograft dysfunction derived from plasma and bronchoalveolar lavage samples might facilitate diagnosis and ultimately improve allograft survival. This review article gives an overview of ...

    journal_title:Respiratory research

    pub_type: 杂志文章,评审


    authors: Speck NE,Schuurmans MM,Murer C,Benden C,Huber LC

    更新日期:2016-06-21 00:00:00

  • The inhibition of human lung fibroblast proliferation and differentiation by Gs-coupled receptors is not predicted by the magnitude of cAMP response.

    abstract:BACKGROUND:Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive fibrotic lung disease for which there is no cure. Current therapeutics are only able to slow disease progression, therefore there is a need to explore alternative, novel treatment options. There is increasing evidence that the 3', 5' cyclic ade...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Roberts MJ,Broome RE,Kent TC,Charlton SJ,Rosethorne EM

    更新日期:2018-04-07 00:00:00

  • Fluticasone furoate: once-daily evening treatment versus twice-daily treatment in moderate asthma.

    abstract:BACKGROUND:Inhaled corticosteroids are the recommended first-line treatment for asthma but adherence to therapy is suboptimal. The objectives of this study were to compare the efficacy and safety of once-daily (OD) evening and twice-daily (BD) regimens of the novel inhaled corticosteroid fluticasone furoate (FF) in ast...

    journal_title:Respiratory research

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Woodcock A,Bleecker ER,Busse WW,Lötvall J,Snowise NG,Frith L,Jacques L,Haumann B,Bateman ED

    更新日期:2011-12-21 00:00:00

  • Lymphocyte senescence in COPD is associated with loss of glucocorticoid receptor expression by pro-inflammatory/cytotoxic lymphocytes.

    abstract:BACKGROUND:Glucocorticoid (GC) resistance is a major barrier in COPD treatment. We have shown increased expression of the drug efflux pump, Pgp1 in cytotoxic/pro-inflammatory lymphocytes in COPD. Loss of lymphocyte co-stimulatory molecule CD28 (lymphocyte senescence) was associated with a further increase in their pro-...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Hodge G,Jersmann H,Tran HB,Holmes M,Reynolds PN,Hodge S

    更新日期:2015-01-09 00:00:00

  • Surfactant proteins, SP-A and SP-D, in respiratory fungal infections: their role in the inflammatory response.

    abstract::Pulmonary surfactant is a complex fluid that comprises phospholipids and four proteins (SP-A, SP-B, SP-C, and SP-D) with different biological functions. SP-B, SP-C, and SP-D are essential for the lungs' surface tension function and for the organization, stability and metabolism of lung parenchyma. SP-A and SP-D, which...

    journal_title:Respiratory research

    pub_type: 杂志文章,评审


    authors: Carreto-Binaghi LE,Aliouat el M,Taylor ML

    更新日期:2016-06-01 00:00:00

  • Association of mast cells with lung function in chronic obstructive pulmonary disease.

    abstract:BACKGROUND:In asthma, higher chymase positive mast cell (MC-C) numbers are associated with less airway obstruction. In COPD, the distribution of MC-C and tryptase positive mast cells (MC-T) in central and peripheral airways, and their relation with lung function, is unknown. We compared MC-T and MC-C distributions in C...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Gosman MM,Postma DS,Vonk JM,Rutgers B,Lodewijk M,Smith M,Luinge MA,Ten Hacken NH,Timens W

    更新日期:2008-09-10 00:00:00

  • Pectoralis muscle area and mortality in smokers without airflow obstruction.

    abstract:BACKGROUND:Low muscle mass is associated with increased mortality in the general population but its prognostic value in at-risk smokers, those without expiratory airflow obstruction, is unknown. We aimed to test the hypothesis that reduced muscle mass is associated with increased mortality in at-risk smokers. METHODS:...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Diaz AA,Martinez CH,Harmouche R,Young TP,McDonald ML,Ross JC,Han ML,Bowler R,Make B,Regan EA,Silverman EK,Crapo J,Boriek AM,Kinney GL,Hokanson JE,Estepar RSJ,Washko GR

    更新日期:2018-04-10 00:00:00

  • Low-molecular-weight heparin reduces hyperoxia-augmented ventilator-induced lung injury via serine/threonine kinase-protein kinase B.

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    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Li LF,Yang CT,Huang CC,Liu YY,Kao KC,Lin HC

    更新日期:2011-07-05 00:00:00

  • Gender differences in pulmonary arterial hypertension patients with BMPR2 mutation: a meta-analysis.

    abstract:OBJECTIVE:To investigate the differences in the proportions of BMPR2 mutations in familial hereditary pulmonary arterial hypertension (HPAH) and idiopathic pulmonary arterial hypertension (IPAH) between males and females and the relationship between BMPR2 mutation and PAH severity. METHODS:A computer was used to searc...

    journal_title:Respiratory research

    pub_type: 杂志文章,meta分析


    authors: Ge X,Zhu T,Zhang X,Liu Y,Wang Y,Zhang W

    更新日期:2020-02-06 00:00:00

  • Perforin, granzyme B, and FasL expression by peripheral blood T lymphocytes in emphysema.

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    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Morissette MC,Parent J,Milot J

    更新日期:2007-09-06 00:00:00

  • Influence of viral infection on the relationships between airway cytokines and lung function in asthmatic children.

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    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Lewis TC,Metitiri EE,Mentz GB,Ren X,Carpenter AR,Goldsmith AM,Wicklund KE,Eder BN,Comstock AT,Ricci JM,Brennan SR,Washington GL,Owens KB,Mukherjee B,Robins TG,Batterman SA,Hershenson MB,Community Action Against Asthma S

    更新日期:2018-11-21 00:00:00

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    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Donovan C,Simoons M,Esposito J,Ni Cheong J,Fitzpatrick M,Bourke JE

    更新日期:2014-03-12 00:00:00

  • 24-h bronchodilation and inspiratory capacity improvements with glycopyrrolate/formoterol fumarate via co-suspension delivery technology in COPD.

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    journal_title:Respiratory research

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Reisner C,Gottschlich G,Fakih F,Koser A,Krainson J,Delacruz L,Arora S,Feldman G,Pudi K,Siddiqui S,Orevillo C,Maes A,St Rose E,Martin U

    更新日期:2017-08-18 00:00:00