Cigarette smoke exposure inhibits extracellular MMP-2 (gelatinase A) activity in human lung fibroblasts.

Abstract:

BACKGROUND:Exposure to cigarette smoke is considered a major risk factor for the development of lung diseases, since its causative role has been assessed in the induction and maintenance of an inflamed state in the airways. Lung fibroblasts can contribute to these processes, due to their ability to produce proinflammatory chemotactic molecules and extracellular matrix remodelling proteinases. Among proteolytic enzymes, gelatinases A and B have been studied for their role in tissue breakdown and mobilisation of matrix-derived signalling molecules. Multiple reports linked gelatinase deregulation and overexpression to the development of inflammatory chronic lung diseases such as COPD. METHODS:In this study we aimed to determine variations in the gelatinolytic pattern of human lung fibroblasts (HFL-1 cell line) exposed to cigarette smoke extract (CSE). Gelatinolytic activity levels were determined by using gelatin zymography for the in-gel detection of the enzymes (proenzyme and activated forms), and the subsequent semi-quantitative densitometric evaluation of lytic bands. Expression of gelatinases was evaluated also by RT-PCR, zymography of the cell lysates and by western blotting. RESULTS:CSE exposure at the doses used (1-10%) did not exert any significant cytotoxic effects on fibroblasts. Zymographic analysis showed that CSE exposure resulted in a linear decrease of the activity of gelatinase A. Control experiments allowed excluding a direct inhibitory effect of CSE on gelatinases. Zymography of cell lysates confirmed the expression of MMP-2 in all conditions. Semi-quantitative evaluation of mRNA expression allowed assessing a reduced transcription of the enzyme, as well as an increase in the expression of TIMP-2. Statistical analyses showed that the decrease of MMP-2 activity in conditioned media reached the statistical significance (p = 0.0031 for 24 h and p = 0.0012 for 48 h), while correlation analysis showed that this result was independent from CSE cytotoxicity (p = 0.7833 for both exposures). CONCLUSION:Present work describes for the first time that, apart well characterized proinflammatory responses, human lung fibroblasts may react to CSE with a significant reduction of extracellular MMP-2 lytic activity. Therefore, fibroblasts may actively participate to the alteration of the proteolysis/antiproteolysis balance, which reflects the defective repair of the extracellular matrix. Such event should provide a further contribution to the maintenance of the inflamed state in the lungs.

journal_name

Respir Res

journal_title

Respiratory research

authors

La Rocca G,Anzalone R,Magno F,Farina F,Cappello F,Zummo G

doi

10.1186/1465-9921-8-23

subject

Has Abstract

pub_date

2007-03-12 00:00:00

pages

23

eissn

1465-9921

issn

1465-993X

pii

1465-9921-8-23

journal_volume

8

pub_type

杂志文章
  • Impact of gender on COPD expression in a real-life cohort.

    abstract::Reports regarding gender-related differences in COPD expression have provided conflicting results. In the French Initiatives BPCO real-world cohort, which contained 688 patients (146 women) when data were extracted, women were matched with men (1:3 ratio: n = 107:275) on age (5-year intervals) and FEV1 (5% predicted i...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-15-20

    authors: Roche N,Deslée G,Caillaud D,Brinchault G,Court-Fortune I,Nesme-Meyer P,Surpas P,Escamilla R,Perez T,Chanez P,Pinet C,Jebrak G,Paillasseur JL,Burgel PR,INITIATIVES BPCO Scientific Committee.

    更新日期:2014-02-17 00:00:00

  • Impact of interleukin-6 on hypoxia-induced pulmonary hypertension and lung inflammation in mice.

    abstract:BACKGROUND:Inflammation may contribute to the pathogenesis of various forms of pulmonary hypertension (PH). Recent studies in patients with idiopathic PH or PH associated with underlying diseases suggest a role for interleukin-6 (IL-6). METHODS:To determine whether endogenous IL-6 contributes to mediate hypoxic PH and...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-10-6

    authors: Savale L,Tu L,Rideau D,Izziki M,Maitre B,Adnot S,Eddahibi S

    更新日期:2009-01-27 00:00:00

  • Increased plasma mannose binding lectin levels are associated with bronchiolitis obliterans after lung transplantation.

    abstract:BACKGROUND:Long-term lung allograft survival is limited by bronchiolitis obliterans syndrome (BOS). Mannose binding lectin (MBL) belongs to the innate immune system, participates in complement activation, and may predispose to graft rejection. We investigated mannose binding (MBL) during cold ischemia and in tissue sam...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-13-56

    authors: Budd SJ,Aris RM,Medaiyese AA,Tilley SL,Neuringer IP

    更新日期:2012-07-04 00:00:00

  • Budesonide/formoterol and formoterol provide similar rapid relief in patients with acute asthma showing refractoriness to salbutamol.

    abstract:BACKGROUND:To compare the efficacy and safety of budesonide/formoterol (Symbicort) with formoterol (Oxis) in the treatment of patients with acute asthma who showed evidence of refractoriness to short-acting beta2-agonist therapy. METHODS:In a 3 hour, randomized, double-blind study, a total of 115 patients with acute a...

    journal_title:Respiratory research

    pub_type: 杂志文章,随机对照试验

    doi:10.1186/1465-9921-7-13

    authors: Bateman ED,Fairall L,Lombardi DM,English R

    更新日期:2006-01-24 00:00:00

  • Greater severity of new onset asthma in allergic subjects who smoke: a 10-year longitudinal study.

    abstract:BACKGROUND:Little is known about the association between cigarette smoking and asthma severity. We assessed smoking as a determinant of disease severity and control in a cohort of clinic-referred allergic subjects who developed new onset asthma. METHODS:Allergic rhinitis subjects with no asthma (n = 371) were followed...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-12-16

    authors: Polosa R,Russo C,Caponnetto P,Bertino G,Sarvà M,Antic T,Mancuso S,Al-Delaimy WK

    更新日期:2011-01-24 00:00:00

  • CASCADE: a phase 2, randomized, double-blind, placebo-controlled, parallel-group trial to evaluate the effect of tezepelumab on airway inflammation in patients with uncontrolled asthma.

    abstract:BACKGROUND:Patients with severe, uncontrolled asthma, particularly those with a non-eosinophilic phenotype, have a great unmet need for new treatments that act on a broad range of inflammatory pathways in the airway. Tezepelumab is a human monoclonal antibody that blocks the activity of thymic stromal lymphopoietin, an...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-020-01513-x

    authors: Emson C,Diver S,Chachi L,Megally A,Small C,Downie J,Parnes JR,Bowen K,Colice G,Brightling CE

    更新日期:2020-10-13 00:00:00

  • Phenotyping community-acquired pneumonia according to the presence of acute respiratory failure and severe sepsis.

    abstract:BACKGROUND:Acute respiratory failure (ARF) and severe sepsis (SS) are possible complications in patients with community-acquired pneumonia (CAP). The aim of the study was to evaluate prevalence, characteristics, risk factors and impact on mortality of hospitalized patients with CAP according to the presence of ARF and ...

    journal_title:Respiratory research

    pub_type: 杂志文章,多中心研究

    doi:10.1186/1465-9921-15-27

    authors: Aliberti S,Brambilla AM,Chalmers JD,Cilloniz C,Ramirez J,Bignamini A,Prina E,Polverino E,Tarsia P,Pesci A,Torres A,Blasi F,Cosentini R

    更新日期:2014-03-04 00:00:00

  • IL13 gene polymorphisms modify the effect of exposure to tobacco smoke on persistent wheeze and asthma in childhood, a longitudinal study.

    abstract:BACKGROUND:Tobacco smoke and genetic susceptibility are risk factors for asthma and wheezing. The aim of this study was to investigate whether there is a combined effect of interleukin-13 gene (IL13) polymorphisms and tobacco smoke on persistent childhood wheezing and asthma. METHODS:In the Isle of Wight birth cohort ...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-9-2

    authors: Sadeghnejad A,Karmaus W,Arshad SH,Kurukulaaratchy R,Huebner M,Ewart S

    更新日期:2008-01-10 00:00:00

  • HIF-1 expression is associated with CCL2 chemokine expression in airway inflammatory cells: implications in allergic airway inflammation.

    abstract:BACKGROUND:The pathogenesis of allergic airway inflammation in asthmatic patients is complex and characterized by cellular infiltrates and activity of many cytokines and chemokines. Both the transcription factor hypoxia inducible factor-1 (HIF-1) and chemokine CCL2 have been shown to play pivotal roles in allergic airw...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-13-60

    authors: Baay-Guzman GJ,Bebenek IG,Zeidler M,Hernandez-Pando R,Vega MI,Garcia-Zepeda EA,Antonio-Andres G,Bonavida B,Riedl M,Kleerup E,Tashkin DP,Hankinson O,Huerta-Yepez S

    更新日期:2012-07-23 00:00:00

  • Viruses, dendritic cells and the lung.

    abstract::The interaction between viruses and dendritic cells (DCs) is varied and complex. DCs are key elements in the development of a host response to pathogens such as viruses, but viruses have developed survival tactics to either evade or diminish the immune system that functions to kill and eliminate these micro-organisms....

    journal_title:Respiratory research

    pub_type: 杂志文章,评审

    doi:10.1186/rr63

    authors: Peebles RS Jr,Graham BS

    更新日期:2001-01-01 00:00:00

  • Pathophysiological characterization of asthma transitions across adolescence.

    abstract:BACKGROUND:Adolescence is a period of change, which coincides with disease remission in a significant proportion of subjects with childhood asthma. There is incomplete understanding of the changing characteristics underlying different adolescent asthma transitions. We undertook pathophysiological characterization of tr...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-014-0153-7

    authors: Arshad SH,Raza A,Lau L,Bawakid K,Karmaus W,Zhang H,Ewart S,Patil V,Roberts G,Kurukulaaratchy R

    更新日期:2014-11-29 00:00:00

  • Collaborative interactions between neutrophil elastase and metalloproteinases in extracellular matrix degradation in three-dimensional collagen gels.

    abstract:BACKGROUND:Extended culture of monocytes and fibroblasts in three-dimensional collagen gels leads to degradation of the gels (see linked study in this issue, "Fibroblasts and monocytes contract and degrade three-dimensional collagen gels in extended co-culture"). The current study, therefore, was designed to evaluate p...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/rr73

    authors: Zhu Y,Liu X,Sköld CM,Wang H,Kohyama T,Wen FQ,Ertl RF,Rennard SI

    更新日期:2001-01-01 00:00:00

  • TLR4 signaling is essential for survival in acute lung injury induced by virulent Pseudomonas aeruginosa secreting type III secretory toxins.

    abstract:BACKGROUND:The relative contributions of the cytotoxic phenotype of P. aeruginosa expressing type III secretory toxins and an immunocompromised condition lacking normal Toll-like receptor 4 (TLR4) signaling in the pathogenesis of acute lung injury and sepsis were evaluated in a mouse model for Pseudomonas aeruginosa pn...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-5-1

    authors: Faure K,Sawa T,Ajayi T,Fujimoto J,Moriyama K,Shime N,Wiener-Kronish JP

    更新日期:2004-02-12 00:00:00

  • Lung function reductions associated with motor vehicle density in chronic obstructive pulmonary disease: a cross-sectional study.

    abstract:BACKGROUND:Motor vehicle-related air pollution can potentially impair lung function. The effect of pollution in people with compromised pulmonary function such as in COPD has not been previously investigated. To examine the association of lung function with motor vehicle density in people with spirometrically determine...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-016-0451-3

    authors: Nitschke M,Appleton SL,Li Q,Tucker GR,Shah P,Bi P,Pisaniello DL,Adams RJ

    更新日期:2016-10-24 00:00:00

  • CD8 positive T cells express IL-17 in patients with chronic obstructive pulmonary disease.

    abstract:BACKGROUND:Chronic obstructive pulmonary disease (COPD) is a progressive and irreversible chronic inflammatory disease of the lung. The nature of the immune reaction in COPD raises the possibility that IL-17 and related cytokines may contribute to this disorder. This study analyzed the expression of IL-17A and IL-17F a...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-12-43

    authors: Chang Y,Nadigel J,Boulais N,Bourbeau J,Maltais F,Eidelman DH,Hamid Q

    更新日期:2011-04-10 00:00:00

  • Trends in prevalence and incidence of chronic respiratory diseases from 1990 to 2017.

    abstract:BACKGROUND:Chronic respiratory diseases (CRDs) are leading causes of morbidity worldwide. However, the spatial and temporal trends in prevalence and incidence of CRDs have not been estimated. METHODS:Based on data from the Global Burden of Diseases, Injuries, and Risk Factors Study 2017, we analyzed the prevalence and...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-020-1291-8

    authors: Xie M,Liu X,Cao X,Guo M,Li X

    更新日期:2020-02-11 00:00:00

  • Pectoralis muscle area and mortality in smokers without airflow obstruction.

    abstract:BACKGROUND:Low muscle mass is associated with increased mortality in the general population but its prognostic value in at-risk smokers, those without expiratory airflow obstruction, is unknown. We aimed to test the hypothesis that reduced muscle mass is associated with increased mortality in at-risk smokers. METHODS:...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-018-0771-6

    authors: Diaz AA,Martinez CH,Harmouche R,Young TP,McDonald ML,Ross JC,Han ML,Bowler R,Make B,Regan EA,Silverman EK,Crapo J,Boriek AM,Kinney GL,Hokanson JE,Estepar RSJ,Washko GR

    更新日期:2018-04-10 00:00:00

  • Predictive factors of severe coronavirus disease 2019 in previously healthy young adults: a single-center, retrospective study.

    abstract:BACKGROUND:Several previously healthy young adults have developed Coronavirus Disease 2019 (COVID-19), and a few of them progressed to the severe stage. However, the factors are not yet determined. METHOD:We retrospectively analyzed 123 previously healthy young adults diagnosed with COVID-19 from January to March 2020...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-020-01412-1

    authors: Zhou C,Huang Z,Tan W,Li X,Yin W,Xiao Y,Tao Z,Geng S,Hu Y

    更新日期:2020-06-22 00:00:00

  • Profibrotic role of WNT10A via TGF-β signaling in idiopathic pulmonary fibrosis.

    abstract:BACKGROUND:WNT/β-catenin signaling plays an important role in the pathogenesis of idiopathic pulmonary fibrosis (IPF); however, the role of WNT10A via transforming growth factor (TGF)-β signaling remains unclear. METHODS:We evaluated the expression of WNT10A and TGF-β in bleomycin (BLM)-treated mice and the interactio...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-016-0357-0

    authors: Oda K,Yatera K,Izumi H,Ishimoto H,Yamada S,Nakao H,Hanaka T,Ogoshi T,Noguchi S,Mukae H

    更新日期:2016-04-12 00:00:00

  • Fractal ventilation enhances respiratory sinus arrhythmia.

    abstract:BACKGROUND:Programming a mechanical ventilator with a biologically variable or fractal breathing pattern (an example of 1/f noise) improves gas exchange and respiratory mechanics. Here we show that fractal ventilation increases respiratory sinus arrhythmia (RSA) -- a mechanism known to improve ventilation/perfusion mat...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-6-41

    authors: Mutch WA,Graham MR,Girling LG,Brewster JF

    更新日期:2005-05-09 00:00:00

  • Oxidative stress mediates the apoptosis and epigenetic modification of the Bcl-2 promoter via DNMT1 in a cigarette smoke-induced emphysema model.

    abstract:BACKGROUND:Emphysema is a crucial pathological characteristic of chronic obstructive pulmonary disease (COPD). Oxidative stress, apoptosis and epigenetic mechanisms contribute to the pathogenesis of emphysema. However, an attempt to accurately identify whether these mechanisms interact with each other and how they are ...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-020-01495-w

    authors: Zeng H,Li T,He X,Cai S,Luo H,Chen P,Chen Y

    更新日期:2020-09-03 00:00:00

  • A candidate gene identification strategy utilizing mouse to human big-data mining: "3R-tenet" in COPD genetic research.

    abstract:BACKGROUND:Early life impairments leading to lower lung function by adulthood are considered as risk factors for chronic obstructive pulmonary disease (COPD). Recently, we compared the lung transcriptomic profile between two mouse strains with extreme total lung capacities to identify plausible pulmonary function deter...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-018-0795-y

    authors: Vishweswaraiah S,George L,Purushothaman N,Ganguly K

    更新日期:2018-06-06 00:00:00

  • Five novel clinical phenotypes for critically ill patients with mechanical ventilation in intensive care units: a retrospective and multi database study.

    abstract:BACKGROUND:Although protective mechanical ventilation (MV) has been used in a variety of applications, lung injury may occur in both patients with and without acute respiratory distress syndrome (ARDS). The purpose of this study is to use machine learning to identify clinical phenotypes for critically ill patients with...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-020-01588-6

    authors: Su L,Zhang Z,Zheng F,Pan P,Hong N,Liu C,He J,Zhu W,Long Y,Liu D

    更新日期:2020-12-10 00:00:00

  • Soluble receptor for advanced glycation end products in COPD: relationship with emphysema and chronic cor pulmonale: a case-control study.

    abstract:BACKGROUND:The receptor for advanced glycation end products (RAGE) is a multiligand signal transduction receptor that can initiate and perpetuate inflammation. Its soluble isoform (sRAGE) acts as a decoy receptor for RAGE ligands, and is thought to afford protection against inflammation. With the present study, we aime...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-12-37

    authors: Miniati M,Monti S,Basta G,Cocci F,Fornai E,Bottai M

    更新日期:2011-03-30 00:00:00

  • Weighing the evidence for pharmacological treatment interventions in mild COPD; a narrative perspective.

    abstract::There is increasing focus on understanding the nature of chronic obstructive pulmonary disease (COPD) during the earlier stages. Mild COPD (Global Initiative for Chronic Obstructive Lung Disease [GOLD] stage 1 or the now-withdrawn GOLD stage 0) represents an early stage of COPD that may progress to more severe disease...

    journal_title:Respiratory research

    pub_type: 杂志文章,评审

    doi:10.1186/s12931-019-1108-9

    authors: Singh D,D'Urzo AD,Donohue JF,Kerwin EM

    更新日期:2019-07-08 00:00:00

  • Pioglitazone is as effective as dexamethasone in a cockroach allergen-induced murine model of asthma.

    abstract:BACKGROUND:While glucocorticoids are currently the most effective therapy for asthma, associated side effects limit enthusiasm for their use. Peroxisome proliferator-activated receptor-gamma (PPAR-gamma) activators include the synthetic thiazolidinediones (TZDs) which exhibit anti-inflammatory effects that suggest usef...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-8-90

    authors: Narala VR,Ranga R,Smith MR,Berlin AA,Standiford TJ,Lukacs NW,Reddy RC

    更新日期:2007-12-04 00:00:00

  • Interleukin-17 regulation: an attractive therapeutic approach for asthma.

    abstract::Interleukin (IL)-17 is recognized to play a critical role in numerous immune and inflammatory responses by regulating the expression of various inflammatory mediators, which include cytokines, chemokines, and adhesion molecules. There is growing evidence that IL-17 is involved in the pathogenesis of asthma. IL-17 orch...

    journal_title:Respiratory research

    pub_type: 杂志文章,评审

    doi:10.1186/1465-9921-11-78

    authors: Park SJ,Lee YC

    更新日期:2010-06-16 00:00:00

  • Cigarette smoke-induced accumulation of lung dendritic cells is interleukin-1α-dependent in mice.

    abstract:BACKGROUND:Evidence suggests that dendritic cells accumulate in the lungs of COPD patients and correlate with disease severity. We investigated the importance of IL-1R1 and its ligands IL-1α and β to dendritic cell accumulation and maturation in response to cigarette smoke exposure. METHODS:Mice were exposed to cigare...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-13-81

    authors: Botelho FM,Nikota JK,Bauer CM,Morissette MC,Iwakura Y,Kolbeck R,Finch D,Humbles AA,Stämpfli MR

    更新日期:2012-09-19 00:00:00

  • Distinctive lung function trajectories from age 10 to 26 years in men and women and associated early life risk factors - a birth cohort study.

    abstract::Pre-bronchodilator lung function including forced vital capacity (FVC), forced expiratory flow in 1 second (FEV1), their ratio (FEV1/FVC), and forced expiratory flow 25-75% (FEF25-75) measured at age 10, 18, and 26 years in the Isle of Wight birth cohort was analyzed for developmental patterns (trajectories). Early li...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-019-1068-0

    authors: Karmaus W,Mukherjee N,Janjanam VD,Chen S,Zhang H,Roberts G,Kurukulaaratchy RJ,Arshad H

    更新日期:2019-05-22 00:00:00

  • Intermittent hypoxia promotes melanoma lung metastasis via oxidative stress and inflammation responses in a mouse model of obstructive sleep apnea.

    abstract:BACKGROUND:Recently, increased tumor incidence and cancer-related mortality have been reported among patients with obstructive sleep apnea (OSA). Intermittent hypoxia (IH), the hallmark feature of OSA, contributes to the metastasis of tumors. However, the molecular mechanisms by which tumor metastasis is accelerated by...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-018-0727-x

    authors: Li L,Ren F,Qi C,Xu L,Fang Y,Liang M,Feng J,Chen B,Ning W,Cao J

    更新日期:2018-02-12 00:00:00