Abstract:
:The complete nucleotide sequence of the gene for a cell-surface protein antigen (PAc) of Streptococcus mutans MT8148 (serotype c) was determined. The pac gene consisted of 4695 bp and coded for a 170773D protein. The pac gene product contained a putative 38 amino acid signal peptide, resulting in a 166817D mature protein. A potential promoter sequence and a putative Shine-Dalgarno sequence preceded the open reading frame. Two internal repeating amino acid sequences were present in the PAc. One repeating region located in the N-terminal region was rich in alanine, and the other located in the central region was rich in proline. Southern blot analysis under the less stringent condition (allowing up to 35% base mismatch) revealed that the probe covering the proline-rich region hybridized to DNA preparations from strains of Streptococcus cricetus, Streptococcus sobrinus and Streptococcus downei as well as Streptococcus mutans.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Okahashi N,Sasakawa C,Yoshikawa M,Hamada S,Koga Tdoi
10.1111/j.1365-2958.1989.tb00215.xsubject
Has Abstractpub_date
1989-05-01 00:00:00pages
673-8issue
5eissn
0950-382Xissn
1365-2958journal_volume
3pub_type
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