Abstract:
OBJECTIVES:Our aim was to establish and characterize a novel pancreatic ductal adenocarcinoma cell line from a patient in whom the origin of the invasive carcinoma could be traced back to the intraductal papillary mucinous neoplasm (IPMN) precursor lesion. METHODS:The primary patient-derived tumor was propagated in immunocompromised mice for 2 generations and used to establish a continuous in vitro culture termed ASAN-PaCa. Transplantation to fertilized chicken eggs confirmed the tumorigenic potential in vivo. Molecular analyses included karyotyping, next-generation genomic sequencing, expression analysis of marker proteins, and mucin-profiling. RESULTS:The analysis of marker proteins confirmed the epithelial nature of the established cell line, and revealed that the expression of the mucin MUC1 was higher than that of MUC2 and MUC5AC. ASAN-PaCa cells showed rapid in vitro and in vivo growth and multiple chromosomal aberrations. They harbored mutations in KRAS (Q61H), TP53 (Y220C), and RNF43 (I47V and L418M) but lacked either IPMN-specific GNAS or presumed pancreatic ductal adenocarcinoma-driving mutations in KRAS (codons 12/13), SMAD, and CDKN2A genes. CONCLUSIONS:ASAN-PaCa cell line represents a novel preclinical model of pancreatic adenocarcinoma arising in the background of IPMN, and offers an opportunity to study how further introduction of known driver mutations might contribute to pancreatic carcinogenesis.
journal_name
Pancreasjournal_title
Pancreasauthors
Heller A,Angelova AL,Bauer S,Grekova SP,Aprahamian M,Rommelaere J,Volkmar M,Janssen JW,Bauer N,Herr I,Giese T,Gaida MM,Bergmann F,Hackert T,Fritz S,Giese NAdoi
10.1097/MPA.0000000000000673subject
Has Abstractpub_date
2016-11-01 00:00:00pages
1452-1460issue
10eissn
0885-3177issn
1536-4828journal_volume
45pub_type
杂志文章相关文献
PANCREAS文献大全abstract:INTRODUCTION AND AIMS:It has been proposed that distinct tertiary structures of the C-terminus of CCK-8 and CCK-58 result in differences in stimulation of pancreatic amylase secretion. Binding of CCK-8 and CCK-58 to CCK-A and CCK-B receptors and stability to enzymatic digestion were used as independent probes for terti...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-200210000-00021
更新日期:2002-10-01 00:00:00
abstract:OBJECTIVES:IQ motif containing GTPase-activating protein 1 (IQGAP1) acts as a scaffold for aberrant mitogen-activated protein kinase (MAPK) signaling driven by KRAS mutations in pancreatic ductal adenocarcinoma (PDAC). We determined the role of IQGAP1 in clonogenic growth and metastasis in PDAC. METHODS:We inhibited I...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000001198
更新日期:2019-01-01 00:00:00
abstract::A comparative study of antibacterial activity of pancreatic juice was conducted on six mammalian species. Pancreatic juice collections were conducted as acute (rabbit, guinea pig, rat) and chronic (pig, sheep, cattle) experiments, in the former before and after stimulation [cholecystokinin (CCK) and secretin] and in t...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199309000-00003
更新日期:1993-09-01 00:00:00
abstract::Reports of a so-called "mucin-producing tumor of the pancreas" are increasing worldwide. Although the clinicopathologic features and therapeutic strategies of this tumor have been enthusiastically investigated, there are still many unanswered questions regarding this ailment. In this study, problems in the diagnosis a...
journal_title:Pancreas
pub_type: 杂志文章,评审
doi:10.1097/00006676-199804000-00025
更新日期:1998-04-01 00:00:00
abstract:OBJECTIVES:We evaluated whether neoadjuvant therapy followed by surgical resection improves the clinical outcome for patients with borderline resectable pancreatic cancer with radiologic artery involvement (BRPC-A). METHODS:We reviewed 143 BRPC-A patients from among 330 pancreatic cancer patients, including 111 potent...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000634
更新日期:2016-11-01 00:00:00
abstract::Several reports have described a loss of endogenous antioxidants and molecular oxidative damage during acute pancreatitis. Since hyaluronic acid and chondroitin-4-sulfate possess antioxidant properties, the effect of the administration of these glycosaminoglycans in a cerulein-induced acute pancreatitis in rats was in...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-200403000-00019
更新日期:2004-03-01 00:00:00
abstract:OBJECTIVES:Pancreatic stellate cells (PSCs) play a crucial role during pancreatic fibrosis development. Hydrogen sulfide (H2S) is a recently discovered gaseous transmitter, whose role in PSCs has not been explored yet. In the present study, we examined the effects of sodium hydrosulfide (NaHS), an H2S donor, on rat PSC...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e318223645b
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVES:Pancreatic anastomotic leakage remains a major troublesome complication after pancreaticoduodenectomy. Thus, various technical modifications regarding the pancreatic anastomosis after pancreaticoduodenectomy have been attempted to minimize anastomotic leakage. We have performed duct-to-mucosa pancreaticojeju...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e3180676dc2
更新日期:2007-10-01 00:00:00
abstract:OBJECTIVES:A selective therapy for pancreatitis is total pancreatectomy and islet autotransplantation. Outcomes and geographical variability of patients who had total pancreatectomy (TP) alone or total pancreatectomy with islet autotransplantation (TPIAT) were assessed. METHODS:Data were obtained from the Healthcare C...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000001405
更新日期:2019-10-01 00:00:00
abstract:OBJECTIVES:To assess the degree of interobserver agreement of MRI in the diagnostic assessment of pancreatic cysts (PCs). METHODS:Magnetic resonance imaging sets of images of 62 patients with PCs (32 with histological confirmation and 30 with clinical diagnosis) were reviewed by 4 experienced radiologists. Features sc...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e31822899b6
更新日期:2012-03-01 00:00:00
abstract::Although ethanol abuse is the major etiologic factor in the development of acute and chronic pancreatitis, the mechanisms of ethanol effects to cause pancreatitis are poorly understood. The major reason for the lack of progress is the relative lack of animal models that reproduce the deleterious effects of ethanol on ...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-200311000-00004
更新日期:2003-11-01 00:00:00
abstract:OBJECTIVE:All forms of commonly practiced enteral feeding techniques stimulate pancreatic secretion, and only intravenous feeding avoids it. In this study, we explored the possibility of more distal enteral infusions of tube feeds to see whether activation of the ileal brake mechanism can result in enteral feeding with...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/01.mpa.0000183374.11919.e5
更新日期:2005-11-01 00:00:00
abstract:OBJECTIVES:Pancreatic ductal adenocarcinoma remains as a chemoresistant disease with the poorest prognosis. Gemcitabine has been the standard treatment during the last decade. Erlotinib, a tyrosine kinase inhibitor, in combination with gemcitabine produces a small increase in survival. However, these results remain ins...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000452
更新日期:2016-02-01 00:00:00
abstract:OBJECTIVES:In patients with chronic pancreatitis (cP) with an inflammatory mass in the pancreatic head, the degree of fibrosis in the pancreatic head compared with the tail should be determined and differences in the expression pattern of collagen types I, III, and IV; laminin; vitronectin; and fibronectin should be ev...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-200403000-00009
更新日期:2004-03-01 00:00:00
abstract:OBJECTIVES:This analysis investigated nomogram use to evaluate metastatic pancreatic cancer prognosis. METHODS:Thirty-four baseline factors were examined in the Metastatic Pancreatic Adenocarcinoma Clinical Trial (MPACT) (nab-paclitaxel plus gemcitabine vs gemcitabine) data set. Factors significantly (P < 0.1) associa...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000001563
更新日期:2020-07-01 00:00:00
abstract:OBJECTIVES:We examined how lipotoxicity contributes to pancreatic beta-cell secretory dysfunction. METHODS:Effects of palmitate (0.2 mmol/L) were assessed on insulin secretion and soluble N-ethylmaleimide-sensitive factor attachment protein receptor exocytotic machinery in isolated pancreatic islets of type 2 diabetic...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e3180304825
更新日期:2007-04-01 00:00:00
abstract:OBJECTIVES:The contribution of overexpressed microRNA-21 and -221 (miR-21 and miR-221) to the malignant phenotype was determined by inhibiting these miRNAs using antisense oligonucleotides. METHODS:The effects of antisense to miR-21 and miR-221 on cell proliferation, cell cycle arrest, induction of apoptosis, combinat...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e3181ba82e1
更新日期:2009-10-01 00:00:00
abstract:OBJECTIVES:Clinical and morphological criteria are not precise enough to diagnose early chronic pancreatitis (CP). We investigated if short endoscopic pancreas function testing as a part of routine upper endoscopy could improve clinical diagnostics. METHODS:Patients with suspected CP underwent modified secretin-stimul...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e3182847a86
更新日期:2013-10-01 00:00:00
abstract:OBJECTIVES:This study aims to identify the computed tomography (CT) features that may differentiate nonfunctioning pancreatic neuroendocrine tumors (NF-PanNETs) with lymph node (LN) metastasis from NF-PanNETs without lymph node metastasis. METHODS:We retrospectively analyzed 166 NF-PanNETs in 166 patients who had unde...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000888
更新日期:2017-09-01 00:00:00
abstract::Amylase inhibition has gastrointestinal and metabolic effects that may aid in the treatment of diabetes and obesity. We tested whether 4 g of a commercially available wheat amylase inhibitor (WAI) affected postprandial carbohydrate (CHO) absorption and plasma glucose or hormones. Twelve persons (four lean and four obe...
journal_title:Pancreas
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00006676-199808000-00011
更新日期:1998-08-01 00:00:00
abstract:OBJECTIVES:In the hamster model, pancreatic ductal adenocarcinoma develops after treatment with N-nitrosobis-(2-oxopropyl)amino (BOP). In this model, Langerhans islets play a central role in carcinogenesis. In contrast, treatment with BOP in rats and mice did not result in cancer development. We investigated whether pa...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e3182016a08
更新日期:2011-03-01 00:00:00
abstract:OBJECTIVE:This study developed a novel protocol for creating an acute necrotizing pancreatitis model in rats using L-arginine. METHODS:Anesthetic laparotomy was performed on the upper abdomen, and the pancreatic parenchyma of Sprague-Dawley rats was injected with 1 mL of sterilized L-arginine solution at 5 different l...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000259
更新日期:2015-03-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to investigate whether 6 candidate serum miRNAs and their interactions with serum folate level were associated with the risk for pancreatic cancer (PC). METHOD:A hospital-based case-control study including 74 incident PC cases and 74 controls was conducted. Serum folate and miRNAs ...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000204
更新日期:2015-01-01 00:00:00
abstract::The last 5 years have seen a dramatic increased interest in the field of exosome biology. Although much is unknown about the role of exosomes in human health and disease, disparate scientific disciplines are recognizing the highly conserved role that exosomes play in fundamental biological processes. Recently, there h...
journal_title:Pancreas
pub_type: 杂志文章,评审
doi:10.1097/MPA.0000000000001203
更新日期:2019-01-01 00:00:00
abstract:OBJECTIVES:Serum pancreastatin is a sensitive and specific diagnostic biomarker in neuroendocrine tumors (NETs). Elevated pancreastatin levels are associated with worse progression-free survival and overall survival in small bowel and pancreatic NETs. In this study, we investigated the clinical significance of elevated...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000572
更新日期:2016-08-01 00:00:00
abstract::The mechanism of tissue alteration in chronic pancreatitis (CP) is still unclear. Different hypotheses have been discussed, including increasing oxidant stress in the acinar cells, often as a result of exposure to xenobiotics. To evaluate the role of oxidative stress in CP, the authors investigated the expression of t...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-200105000-00009
更新日期:2001-05-01 00:00:00
abstract::Adenosine-5'-O-(2-thiodiphosphate) (ADP beta S), a P2y purinergic agonist, has been shown to be a potent insulin secretagogue on the isolated rat pancreas. In the present work the effects of ADP beta S on insulin somatostatin, and glucagon secretions were investigated in dogs. In vivo, in anesthetized fasted dogs, i.v...
journal_title:Pancreas
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00
abstract::The effects of coffee on exocrine pancreatic secretion are unknown but may be important, because a link between chronic stimulation of pancreatic secretion and experimental chemical carcinogenesis and an association between coffee drinking and human pancreatic adenocarcinoma have been reported. We measured exocrine pa...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-198601000-00011
更新日期:1986-01-01 00:00:00
abstract:OBJECTIVES:Pancreatic cancer is a serious disease worldwide for its high mortality. Gemcitabine has become the frontline option for the treatment of this disease since its approval. However, resistance to the drug has been on the rise in recent years. Searching for other chemotherapeutic agents therefore has attracted ...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e3181ce719e
更新日期:2010-08-01 00:00:00
abstract:OBJECTIVES:The aims of this study were to evaluate diagnostic value of endoscopic ultrasound strain ratio elastography in patients with focal pancreatic masses and to determine the cutoff value between the pancreatic malignancies and inflammatory pancreatic masses using reference areas different than those used by othe...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000758
更新日期:2017-03-01 00:00:00