Abstract:
INTRODUCTION::Trypanosoma cruzi is the etiologic agent of Chagas disease in humans, mainly in Latin America. Trypanosome stocks were isolated by hemoculture from patients followed at Evandro Chagas National Institute of Infectious Diseases (FIOCRUZ) and studied using different approaches. METHODS::For species and genotype identification, the stocks were analyzed by parasitological techniques, polymerase chain reaction assays targeted to specific DNA sequences, isoenzyme patterns, besides sequencing of a polymorphic locus of TcSC5D gene (one stock). RESULTS::The isolates presented typical T. cruzi morphology and usually grew well in routine culture media. Metacyclic trypomastigotes were found in cultures or experimentally infected Triatoma infestans. All isolates were pure T. cruzi cultures, presenting typical 330-bp products from kinetoplast DNA minicircles, and 250 or 200-bp amplicons from the mini-exon non-transcribed spacer. Their genetic type assignment was resolved by their isoenzyme profiles. The finding of TcI in one asymptomatic patient from Paraíba was confirmed by the sequencing assay. TcVI was found in two asymptomatic individuals from Bahia and Rio Grande do Sul. TcII was identified in six patients from Pernambuco, Bahia and Minas Gerais, who presented different clinical forms: cardiac (2), digestive with megaesophagus (1), and indeterminate (3). CONCLUSIONS::The main T. cruzi genotypes found in Brazilian chronic patients were identified in this work, including TcI, which is less frequent and usually causes asymptomatic disease, unlike that in other American countries. This study emphasizes the importance of T. cruzi genotyping for possible correlations between the parasite and patient' responses to therapeutic treatment or disease clinical manifestations.
journal_name
Rev Soc Bras Med Tropjournal_title
Revista da Sociedade Brasileira de Medicina Tropicalauthors
Oliveira TD,Santos BN,Galdino TS,Hasslocher-Moreno AM,Bastos OM,Sousa MAdoi
10.1590/0037-8682-0406-2016subject
Has Abstractpub_date
2017-01-01 00:00:00pages
35-43issue
1eissn
0037-8682issn
1678-9849pii
S0037-86822017000100035journal_volume
50pub_type
杂志文章abstract::The frequency with which condoms are used in sex relations by subjects with HIV was determined by interviewing 132 individuals, 82 men and 50 women, most of them from São Paulo state and some from other regions of the country, all of them seen at an outpatient clinic of the School of Medicine in Botucatu. The women we...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822001000200005
更新日期:2001-03-01 00:00:00
abstract:INTRODUCTION:The recent emergence and rapid spread of Zika and Chikungunya fevers in Brazil, occurring simultaneously to a Dengue fever epidemic, together represent major challenges to public health authorities. This study aimed to identify and compare the 2015-2016 spatial diffusion pattern of Zika, Chikungunya, and D...
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journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86821993000400002
更新日期:1993-10-01 00:00:00
abstract::Introduction This study was conducted in Brazil and Colombia,where dengue is endemic and vector control programs use chemical insecticides. Methods We identified knowledge, attitudes, and practices about dengue and determined the infestation levels of Aedes aegypti in one Brazilian and four Colombian communities. Resu...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
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更新日期:2014-11-01 00:00:00
abstract::[This corrects the article doi: 10.1590/0037-8682-0243-2015] [This corrects the article doi: 10.1590/0037-8682-0454-2015]. ...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 已发布勘误
doi:10.1590/0037-8682-0366-2016
更新日期:2016-09-01 00:00:00
abstract:INTRODUCTION:Visceral leishmaniasis (VL) is an expanding zoonosis in Brazil and is becoming urbanized in several Brazilian regions. This study aims to describe the epidemiological features of human and canine VL in the municipality of Montes Claros, State of Minas Gerais, by focusing on their spatial distribution. MET...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822011000500006
更新日期:2011-10-01 00:00:00
abstract::The aim of this study was to describe the epidemiological profile of snakebite accidents in the healthcare macroregion of the north of the State of Minas Gerais, Brazil. Database information on snakebite accidents covering the period from January 2002 to December 2006 was analyzed. It was found that 10,553 cases were ...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822009000500015
更新日期:2009-09-01 00:00:00
abstract:INTRODUCTION:Zoonotic kala-azar, a lethal disease caused by protozoa of the genus Leishmania is considered out of control in parts of the world, particularly in Brazil, where transmission has spread to cities throughout most of the territory and mortality presents an increasing trend. Although a highly debatable measur...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章,评审
doi:10.1590/s0037-86822011005000014
更新日期:2011-03-01 00:00:00
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journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/0037-8682-0009-2015
更新日期:2015-03-01 00:00:00
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journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/0037-8682-0159-2017
更新日期:2018-05-01 00:00:00
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journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822010000100023
更新日期:2010-01-01 00:00:00
abstract:INTRODUCTION:Carbapenemase-resistant enterobacteria that produce the bla NDM gene are found worldwide. However, this is the first report of blaNDM in Klebsiella aerogenes in Brazil. METHODS:The identification of bacterial species was performed using anautomated system and confirmed by biochemical tests, 16S rRNA gene ...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/0037-8682-0262-2020
更新日期:2020-12-21 00:00:00
abstract::An epidemiological survey was carried out in 3,344 people of an urban town in Lagamar, Minas Gerais, Brazil--during 1992-1993, to evaluate the main risk factors related to taeniasis and cysticercosis. A total number of 875 (78.9%) houses were visited and 1080 (32.3%) subjects were clinically examined. Poor sanitary co...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86821998000100009
更新日期:1998-01-01 00:00:00
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journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/0037-8682-0303-2017
更新日期:2018-01-01 00:00:00
abstract::HTLV-1 is the virus that causes T cell lymphoma/leukemia in adults and a neurological disorder known as HTLV-associated myelopathy or tropical spastic paraparesis. One of the transmission means is through contaminated blood and its byproducts. Because of the risk of HTLV-associated infections, screening for HTLV was i...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822007000300005
更新日期:2007-05-01 00:00:00
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journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822009000300003
更新日期:2009-05-01 00:00:00
abstract::We report the first case of cryptococcosis due to Cryptococcus decagattii in an immunocompetent pediatric patient from an indigenous community in Argentina with a successful outcome. Two isolates (blood, cerebrospinal fluid) were genotyped by restriction fragment length polymorphism of the orotidine monophosphate pyro...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type:
doi:10.1590/0037-8682-0419-2018
更新日期:2019-03-28 00:00:00
abstract::An outbreak of American Cutaneous Leishmaniasis (ACL) occurring in the Northeast region of São Paulo state in 1992 is described. After the notification of the 12 human cases, a skin test survey of the local population was carried out in a rural area of the Itupeva municipality. The survey consisted of 144 interviews a...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86821995000300013
更新日期:1995-07-01 00:00:00
abstract:INTRODUCTION:Chagas disease is caused by Trypanosoma cruzi. Wild and perianthropic mammals maintain the infection/transmission cycle, both in their natural habitat and in the peridomestic area. The aim of this paper was to present the results from a study on wild rodents in the central and northern regions of San Luis ...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822010000300007
更新日期:2010-05-01 00:00:00
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journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/0037-8682-1394-2013
更新日期:2013-07-01 00:00:00
abstract::Yellow fever in the Region of Ribeirao Preto at the turn of XIX century: scientific importance and economic repercussion. This historical review describes the bad situation of public health in Brazil during the XIX Century caused by multiple yellow fever outbreaks. The knowledge regarding to yellow fever at that time ...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 历史文章,杂志文章
doi:10.1590/s0037-86821996000100014
更新日期:1996-01-01 00:00:00
abstract:INTRODUCTION:Rapid diagnostic tests (RDTs) are selected based on their performances. Here, we compared the diagnostic performance of different malaria RDTs. METHODS:Febrile patients were tested for malaria using Vikia Malaria Pf/Pan, Meriline-Meriscreen Pf/Pv/Pan, Right Sign Malaria Pf/Pan, and Right Sign Malaria Pf R...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/0037-8682-0274-2019
更新日期:2020-06-22 00:00:00
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journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/0037-8682-0055-2017
更新日期:2018-07-01 00:00:00
abstract::From May 2000 to January 2001 four, 15 hour-long collections of phlebotomine were done to the insect monitoring. The collection was done in an area of infection of American cutaneous leishmaniasis in the basin of the Araguari River in the municipality of Uberlandia (MG). The first collection was made in May (a cold, d...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822005000100005
更新日期:2005-01-01 00:00:00
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journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/0037-8682-1228-2013
更新日期:2013-05-01 00:00:00
abstract::Aedes aegypti (L) (Diptera: Culicidae) was reared in several concentrations of diflubenzuron and methoprene under laboratory conditions in Uberlândia, State of Minas Gerais, southeastern Brazil. Characteristics such as LC50 and LC95, the susceptibility of immature stages of different ages to these insect growth regula...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822007000600002
更新日期:2007-11-01 00:00:00
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journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/0037-8682-0214-2019
更新日期:2020-02-07 00:00:00
abstract:INTRODUCTION:The genus Staphylococcus is of great importance because of its high prevalence in hospital infections and because it presents high rates of resistance to oxacillin and other antimicrobials. Thus, evaluation of the accuracy of the phenotypic methods that are used to determine the profile of antimicrobial re...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822010000300017
更新日期:2010-05-01 00:00:00
abstract::Four specimens of Triatoma vitticeps and one of Panstrongylus geniculatus were caught inside a home in the municipality of São Fidélis, Rio de Janeiro. One of the specimens of Triatoma vitticeps proved positive for infection with Trypanosoma cruzi. Two people living in that home (one adult and one adolescent) presente...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822008000400020
更新日期:2008-07-01 00:00:00
abstract:INTRODUCTION:Coccidioidomycosis, a disease caused by Coccidioides immitis or Coccidioides posadasii, is endemic in arid climatic regions in Northeast Brazil. Its prevalence is higher among young adult males living in rural areas. Existing literature about this disease in Ceará, a Northeast Brazilian state, are scarce. ...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/0037-8682-0249-2020
更新日期:2020-10-21 00:00:00