[Evaluation of methods commonly used in laboratories to determine the susceptibility to oxacillin among Staphylococcus sp samples isolated from a hospital in Vitória, State of Espírito Santo].

Abstract:

INTRODUCTION:The genus Staphylococcus is of great importance because of its high prevalence in hospital infections and because it presents high rates of resistance to oxacillin and other antimicrobials. Thus, evaluation of the accuracy of the phenotypic methods that are used to determine the profile of antimicrobial resistance is essential to ensure that the most appropriate therapy is chosen. METHODS:One hundred and fourteen strains of Staphylococcus sp (53 S. aureus and 61 CNS) were used to evaluate the accuracy of the methods of disk diffusion, agar microdilution, oxacillin screening agar and automated systems, in comparison with PCR for investigating resistance to oxacillin. RESULTS:The mecA gene was detected in 48 strains (42.1%), and 27 strains (23.7%) showed discrepant results in at least one of the methods (74.1% of CNS, 25.9% of S. aureus). For S. aureus, with the exception of the Microscan Walkaway, all the methods showed 100% specificity and sensitivity. In relation to CNS, the automated system and cefoxitin disk had lower accuracy. CONCLUSIONS:Use of two methods should be the best option for improved accuracy, especially when the diagnostic laboratory only uses an automated system or oxacillin disk diffusion test. Combination of these methods with others presented almost 100% sensitivity and specificity in our study.

journal_name

Rev Soc Bras Med Trop

authors

Kaiser TD,Pacheco FC,Lima AA,Pereira EM,Santos KR,Nunes AP

doi

10.1590/s0037-86822010000300017

subject

Has Abstract

pub_date

2010-05-01 00:00:00

pages

298-303

issue

3

eissn

0037-8682

issn

1678-9849

pii

S0037-86822010000300017

journal_volume

43

pub_type

杂志文章
  • Disseminated cutaneous sporotrichosis in a patient with AIDS: report of a case.

    abstract::We describe a case report of disseminated cutaneous sporotrichosis as the initial presentation of AIDS in a 24-year-old HIV-positive male patient. He presented multiple ulcerated skin lesions distributed over the face, thorax, legs and arms. Biopsy of one of the cutaneous lesions was suggestive of sporotrichosis and c...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章,评审

    doi:10.1590/s0037-86822002000600018

    authors: Carvalho MT,de Castro AP,Baby C,Werner B,Filus Neto J,Queiroz-Telles F

    更新日期:2002-11-01 00:00:00

  • Serological and molecular detection of Leptospira spp in dogs.

    abstract:INTRODUCTION:This study aimed to detect anti-Leptospira spp antibodies and Leptospira DNA in domestic dogs. METHODS:Blood and urine from 106 dogs were evaluated by microscopic agglutination test (MAT) and polymerase chain reaction (PCR), respectively. RESULTS:Six (5.7%) and one (1%) animals were positive by MAT and P...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:10.1590/0037-8682-0276-2017

    authors: Latosinski GS,Fornazari F,Babboni SD,Caffaro K,Paes AC,Langoni H

    更新日期:2018-05-01 00:00:00

  • [Occurrence of canine visceral leishmaniasis in an agricultural settlement in the State of Mato Grosso do Sul, Brazil].

    abstract::During previous research on phlebotomine fauna in a settlement of the Brazilian National Agrarian Reform Institute (INCRA) on the Bodoquena Range, Mato Grosso do Sul State, Brazil, dogs were observed with clinical aspects suggestive of visceral leishmaniasis. A serological survey to leishmaniasis in 97 dogs, by indire...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:10.1590/s0037-86822001000300014

    authors: Nunes VL,Galati EA,Nunes DB,Zinezzi RO,Savani ES,Ishikawa E,Camargo MC,D'Auria SR,Cristaldo G,Rocha HC

    更新日期:2001-05-01 00:00:00

  • [Detection of new cases of leprosy in the City of Prudentópolis, PR: the analysis from 1998 to 2005].

    abstract::The aim of this study was to evaluate the detection of new cases of leprosy in Prudentópolis, State of Paraná, from 1998 to 2005. Additionally, we investigated the age group and predominant clinical form of leprosy cases. Data were obtained from clinical-epidemiological records of confirmed leprosy cases that were not...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:10.1590/s0037-86822007000500010

    authors: Sanches LA,Pittner E,Sanches HF,Monteiro MC

    更新日期:2007-09-01 00:00:00

  • Prevalence and monthly distribution of head lice using two diagnostic procedures in several age groups in Uberlândia, State of Minas Gerais, Southeastern Brazil.

    abstract::Some epidemiological characteristics of head lice, Pediculus capitis, were studied using two procedures: cut hair analysis and head inspection. Higher prevalence rates were observed in the middle and at the end of the school terms. Both procedures indicated that children were the main reservoir for this type of pedicu...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:10.1590/s0037-86822007000200022

    authors: Borges R,Silva JJ,Rodrigues RM,Mendes J

    更新日期:2007-03-01 00:00:00

  • First case report of cryptococcosis due to Cryptococcus decagattii in a pediatric patient in Argentina.

    abstract::We report the first case of cryptococcosis due to Cryptococcus decagattii in an immunocompetent pediatric patient from an indigenous community in Argentina with a successful outcome. Two isolates (blood, cerebrospinal fluid) were genotyped by restriction fragment length polymorphism of the orotidine monophosphate pyro...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type:

    doi:10.1590/0037-8682-0419-2018

    authors: Berejnoi A,Taverna CG,Mazza M,Vivot M,Isla G,Córdoba S,Davel G

    更新日期:2019-03-28 00:00:00

  • [Enzyme typing of species of the genus Candida isolated from the oral cavity].

    abstract::The production of phospholipase and proteinase exoenzymes was evaluated in seventy nine samples of Candida isolated from the oral cavity of patients with oral lesions characteristic of candidosis and from individuals presenting a clinically normal mouth, attended at the University of Dentistry of Ribeirão Preto USP. A...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:10.1590/s0037-86822000000500005

    authors: Candido RC,Azevedo RV,Komesu MC

    更新日期:2000-09-01 00:00:00

  • [An evaluation of the efficacy of the CSF method for diagnosing intestinal helminthiases].

    abstract::The recently proposed CSF method for diagnosing intestinal helminthiases was compared with the other methods (direct; Faust et al.; spontaneous sedimentation in water; and Kato-Katz) that are routinely for this purpose. The CSF method performed satisfactorily, thus showing that this technique can be adopted for use in...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:10.1590/s0037-86822007000300025

    authors: Alarcón RS,Amato Neto V,Gakiya E,Bezerra RC

    更新日期:2007-05-01 00:00:00

  • Larvicidal efficiency of the mushroom Amanitamuscaria (Agaricales, Amanitaceae) against the mosquito Culexquinquefasciatus (Diptera, Culicidae).

    abstract:INTRODUCTION:We report the larvicidal activity of two formulations from Amanita muscariaagainst Culex quinquefasciatus, as well as the viability of the aqueous extract after storage. METHODS The larvicidal activity of aqueous extract and powder from A. muscaria, and the viability of the aqueous extract after storage, w...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:10.1590/0037-8682-0175-2015

    authors: Cárcamo MC,Carapeto LP,Duarte JP,Bernardi E,Ribeiro PB

    更新日期:2016-02-01 00:00:00

  • Risk factors associated with taeniasis-cysticercosis in Lagamar, Minas Gerais State, Brazil.

    abstract::An epidemiological survey was carried out in 3,344 people of an urban town in Lagamar, Minas Gerais, Brazil--during 1992-1993, to evaluate the main risk factors related to taeniasis and cysticercosis. A total number of 875 (78.9%) houses were visited and 1080 (32.3%) subjects were clinically examined. Poor sanitary co...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:10.1590/s0037-86821998000100009

    authors: Silva-Vergara ML,Prata A,Netto HV,Vieira Cde O,Castro JH,Micheletti LG,Otaño AS,Franquini Júnior J

    更新日期:1998-01-01 00:00:00

  • Polycystic hydatid disease in Brazil. Report of five new human cases and a short review of other published observations.

    abstract::This paper describes five additional Brazilian human cases of polycystic hydatid disease due to Echinococcus vogeli, reviews the previous cases reported in Brazil, including one report of E. oligarthus (20 in total), and some epidemiological aspects of this disease which is no longer a curiosity but rather a problem t...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章,评审

    doi:10.1590/s0037-86821996000300001

    authors: D'Alessandro A,Moraes MA,Raick AN

    更新日期:1996-05-01 00:00:00

  • Changes in the prevalence, incidence and residual risk for HIV and hepatitis C virus in Southern Brazilian blood donors since the implementation of NAT screening.

    abstract:INTRODUCTION:Previous studies have shown high residual risk of transfusing a blood donation contaminated by human immunodeficiency virus (HIV) or hepatitis C virus (HCV) in Brazil and motivated the development of a Brazilian platform for simultaneous detection of both viruses by nucleic acid amplification test (NAT) de...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:10.1590/0037-8682-0133-2014

    authors: Kupek E,Petry A

    更新日期:2014-07-01 00:00:00

  • Evaluation of the therapeutic response of hepatitis C in coinfected patients (HIV/HCV): a study of cases from a hospital for chronic liver diseases in the Eastern Brazilian Amazon.

    abstract:INTRODUCTION:The aim of this study was to evaluate the therapeutic response of hepatitis C in patients coinfected with human immunodeficiency virus (HIV-1). METHODS:A retrospective study of 20 patients coinfected with HIV-1/HCV who were treated in the outpatient liver clinic at the Sacred House of Mercy Foundation Hos...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:10.1590/0037-868215432013

    authors: Amaral Ido S,Móia Lde J,Barbosa MS,Demachki S,Araújo MT,Soares Mdo C

    更新日期:2013-01-01 00:00:00

  • [Visceral leishmaniasis in the municipality of Várzea Grande, State of Mato Grosso, between 1998 and 2007].

    abstract::This study aimed to describe the epidemiology and spread of visceral leishmaniasis in the municipality of Várzea Grande, State of Mato Grosso, Brazil, between 1998 and 2007. Forty-eight human cases were reported, with an incidence rate of up to 11.7 per 100,000 inhabitants, particularly among children and adolescents ...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:10.1590/s0037-86822009000500005

    authors: Missawa NA,Borba JF

    更新日期:2009-09-01 00:00:00

  • American cutaneous leishmaniasis triggered by electrocoagulation.

    abstract::Cutaneous leishmaniasis is usually transmitted by infected phlebotomine sand fly bites that initiate local cutaneous lesions. Few reports in the literature describe other modes of transmission. We report a case of a previously healthy 59-year-old woman who underwent electrocoagulation to remove seborrheic keratosis co...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type:

    doi:10.1590/0037-8682-0306-2017

    authors: Martins SS,Santos AO,Lima BD,Gomes CM,Sampaio RNR

    更新日期:2018-01-01 00:00:00

  • Incidence of hepatitis C in Brazil.

    abstract:INTRODUCTION:Hepatitis C is a public health problem of global dimensions, affecting approximately 200 million people worldwide. The main objective of this study was to estimate the incidence rate of hepatitis C in Brazil during the period between 2001 and 2012. METHODS:An epidemiological, temporal, and descriptive stu...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:10.1590/0037-8682-0230-2015

    authors: Hanus JS,Ceretta LB,Simões PW,Tuon L

    更新日期:2015-11-01 00:00:00

  • [Characterization of Schistosoma mansoni strains by morphometry of adult worms derived from single-sex infection].

    abstract::Mice were infected with only one sex cercaria derived from sympatric strains of Schistosoma mansoni. Adult worms presented significative differences (p < 0.05) regarding suckers, testicular lobes, ovary and thickness of the tegument. Data show that morphometric study of unisexual infection worms can be also used for c...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:10.1590/s0037-86822003000600020

    authors: Machado-Silva JR,Neves RH,Ormond L,Hulstijn M,Gomes DC

    更新日期:2003-11-01 00:00:00

  • [Iron status, malnutrition and acute phase response in HIV-positive patients].

    abstract::Nutritional status and some iron metabolism parameters of acute phase response (APR) positive and APR-negative AIDS patients were studied. Twenty-nine AIDS patients were submitted to 24h food intake recall, anthropometry, and albumin, C-reactive protein (CRP), hemoglobin, ferritin, and total iron binding capacity (TIB...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:10.1590/s0037-86822000000200003

    authors: Monteiro JP,Cunha DF,da Cunha SF,dos Santos VM,Silva-Vergara ML,Correia D,Bianchi ML

    更新日期:2000-03-01 00:00:00

  • [Identification of Malassezia species in patients with pityriasis versicolor in Goiânia-GO].

    abstract::The objectives of this study were to investigate the frequency of pityriasis versicolor occurrence and to identify yeasts of the genus Malassezia in patients at the mycology laboratory of the Federal University of Goiás, in Goiânia, State of Goiás. Ninety-five cases of pityriasis versicolor were diagnosed, and four sp...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:10.1590/s0037-86822006000600017

    authors: Miranda KC,de Araujo CR,Soares AJ,de Aquino Lemos J,Souza LK,do Rosário Rodrigues Silva M

    更新日期:2006-11-01 00:00:00

  • A case report of human intoxication due to a snakebite by the opisthoglyphous dipsadid Thamnodynastes lanei Bailey, Thomas & Silva-Jr, 2005.

    abstract::We present a case of human intoxication due to a snakebite by the opisthoglyphous dipsadid Thamnodynastes lanei. A 26-year-old man was bitten on the right hand and was not medicated. Bleeding lasted a few seconds, while paresthesia, chills, and headache persisted for up to 10 hours. The pain disappeared after a week, ...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type:

    doi:10.1590/0037-8682-0194-2020

    authors: Ganança PHS,Fraga R,Vasconcelos Neto LB,Santos Júnior APD

    更新日期:2020-11-13 00:00:00

  • How effective is dog culling in controlling zoonotic visceral leishmaniasis? A critical evaluation of the science, politics and ethics behind this public health policy.

    abstract:INTRODUCTION:Zoonotic kala-azar, a lethal disease caused by protozoa of the genus Leishmania is considered out of control in parts of the world, particularly in Brazil, where transmission has spread to cities throughout most of the territory and mortality presents an increasing trend. Although a highly debatable measur...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章,评审

    doi:10.1590/s0037-86822011005000014

    authors: Costa CH

    更新日期:2011-03-01 00:00:00

  • Seroprevalence of cytomegalovirus antibodies in blood donors in southern, Brazil.

    abstract:INTRODUCTION:Cytomegalovirus (CMV) infection is a matter of concern for blood bank professionals and blood transfusion recipients, especially in cases of transfusions to neonates and immunocompromised patients. Thus, the present study aimed to determine the seroprevalence of CMV IgG and IgM antibodies among blood donor...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:10.1590/s0037-86822010000400004

    authors: Souza MA,Passos AM,Treitinger A,Spada C

    更新日期:2010-07-01 00:00:00

  • Human platelet antigen-3 polymorphism as a risk factor for rheumatological manifestations in hepatitis C.

    abstract:INTRODUCTION:Hepatitis C virus (HCV) infection is involved in the pathogenesis of autoimmune and rheumatic disorders. Although the human platelet antigens (HPA) polymorphism are associated with HCV persistence, they have not been investigated in rheumatological manifestations (RM). This study focused on verifying assoc...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:10.1590/0037-8682-0210-2019

    authors: Medolago NB,Ferrasi AC,Rocha OMD,Pardini MIMC,Grotto RMT,Galvani AF,Silva GF

    更新日期:2020-01-27 00:00:00

  • Spatial analysis of new cases of leprosy in the State of Espírito Santo, Brazil, between 2004 and 2009.

    abstract:INTRODUCTION:Leprosy in Brazil is a public health issue, and there are many regions in the State of Espírito Santo with high endemic incidence levels of leprosy, characterizing this state as a priority for leprosy programs. The aim of this study was to determine the spatial distribution of coefficients of new cases of ...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:10.1590/s0037-86822012000300019

    authors: Sampaio PB,Rossi TL,Cerutti Junior C,Zandonade E

    更新日期:2012-06-01 00:00:00

  • Seroprevalence of hepatitis B virus infection and seroconvertion to anti-HBsAg in laboratory staff in Goiânia, Goiás.

    abstract::Were analyzed 648 serum samples from laboratory staff in Goiânia, Goiás aiming detection of three serological markers of HBV: HBsAg, anti-HBsAg and anti-HBcAg. The HBsAg and anti-HBcAg positive samples were also analyzed for HBeAg, anti-HBeAg and anti-HBcAgIgM markers. HBV infection rate of 24.1% was observed and, fro...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:10.1590/s0037-86822005000200005

    authors: Silva PA,Fiaccadori FS,Borges AM,Silva SA,Daher RR,Martins RM,Cardoso Dd

    更新日期:2005-03-01 00:00:00

  • Efficacy of voriconazole in experimental rat paracoccidioidomycosis.

    abstract:INTRODUCTION:Amphotericin B, azole or sulfamide drugs are used for treatment of patients with paracoccidioidomycosis. Among the azole drugs, voriconazole was active in vitro against Paracoccidioides brasiliensis and showed efficacy in the treatment of patients infected with this fungus.In the present study the antifung...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:

    authors: Granzoto DS,Vitali LH,Martinez R

    更新日期:2013-01-01 00:00:00

  • Antischistosomal activity of acridanone-hydrazones in Cebus monkeys experimentally infected with the SJ strain of Schistosoma mansoni.

    abstract::In this study, four compounds were utilized at the dose of 12.5 mg/kg body weight, p.o., to treat Cebus monkeys experimentally infected with about 200 cercariae of Schistosoma mansoni (SJ strain), via transcutaneous route. The oograms performed with rectal snips, as well as stool examinations carried out periodically,...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:10.1590/s0037-86821995000300003

    authors: Coelho PM,Pereira LH,de Mello RT

    更新日期:1995-07-01 00:00:00

  • First serologic evidence of human hantavirus infection in Alagoas State in Northeastern Brazil.

    abstract:INTRODUCTION:Hantavirus cardiopulmonary syndrome (HCPS) is rare in Northeastern Brazil. METHODS:Prospective surveillance was conducted over a two-year period in Alagoas State, Northeastern Brazil. The prevalence of anti-hantavirus N-antigen IgM and IgG in human serum samples was determined by enzyme-linked immunosorbe...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:10.1590/0037-8682-0117-2017

    authors: Santos-Júnior JAD,Santos FMD,Barros PA,Medeiros NPT,Sabino-Santos Júnior G,Lima MC,Figueiredo LTM,Borges AA

    更新日期:2017-11-01 00:00:00

  • Prevalence of serum antibodies to hantavirus in a rural population from the Southern State of Santa Catarina, Brazil.

    abstract:INTRODUCTION:Rodent-borne hantaviruses cause severe human diseases. We completed a serological survey of hantavirus infection in rural inhabitants of Turvo County, in the southern State of Santa Catarina, Brazil, in which seropositivity for hantavirus was correlated to previous disease in the participants. METHODS:The...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:10.1590/s0037-86822012000100022

    authors: Pereira GW,Teixeira AM,Souza MS,Braga AD,Santos Junior GS Jr,Figueiredo GG,Figueiredo LT,Borges AA

    更新日期:2012-02-01 00:00:00

  • [Spatial distribution of American tegumentary leishmaniasis cases in Campinas, State of São Paulo, between 1992 and 2003].

    abstract::This is a retrospective study with ecological features that describes the epidemiological pattern and geographical distribution of American tegumentary leishmaniasis cases in Campinas, São Paulo, between 1992 and 2003. The probable infection locations were georeferenced by means of Global Position System and spatially...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:10.1590/s0037-86822009000300013

    authors: Nasser JT,Donalisio MR,Vasconcelos CH

    更新日期:2009-05-01 00:00:00