[Cloning and expression of the transmembranic glycoprotein from human T cell lymphotropic virus in a prokaryotic system].

Abstract:

:HTLV-1 is the virus that causes T cell lymphoma/leukemia in adults and a neurological disorder known as HTLV-associated myelopathy or tropical spastic paraparesis. One of the transmission means is through contaminated blood and its byproducts. Because of the risk of HTLV-associated infections, screening for HTLV was introduced for Brazilian blood donors in 1993. Most of the diagnostic kits used in the national blood banks are bought from foreign companies. Brazil does not have the technology to produce this material and there is a need to produce diagnostic systems with national technology. In this study, we show the expression of gp21/HTLV-1 in Escherichia coli and its reactivity towards monoclonal antibodies and the antibodies of infected patients. Expressing these proteins is the first step towards obtaining diagnostic kits with Brazilian biotechnology.

journal_name

Rev Soc Bras Med Trop

authors

Russo-Carbolante EM,Penteado FC,Medeiros L,Kashima S,Takayanagui OM,Covas DT

doi

10.1590/s0037-86822007000300005

subject

Has Abstract

pub_date

2007-05-01 00:00:00

pages

277-81

issue

3

eissn

0037-8682

issn

1678-9849

pii

S0037-86822007000300005

journal_volume

40

pub_type

杂志文章
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  • Susceptibility of Aedes aegypti (L) to the insect growth regulators diflubenzuron and methoprene in Uberlândia, State of Minas Gerais.

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  • [Prevalence of varicella-zoster virus antibodies in young adults from different Brazilian climatic regions].

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  • Invasive aspergillosis in a user of inhaled cocaine: rhinosinusitis with bone and cartilage destruction.

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  • [Destruction of Strongyloides venezuelensis infective larvae by fungi Duddingtonia flagrans, Arthrobotrys robusta and Monacrosporium sinense].

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  • Influence of canine brain decomposition on laboratory diagnosis of rabies.

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  • [Detection of Cyclospora sp oocysts in the feces of stray dogs in Greater São Paulo (São Paulo State, Brazil)].

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  • [Note on the types and specimens of triatomine deposited in the Entomological Collection of Instituto Butantan, São Paulo (Hemiptera:Reduviidae)].

    abstract::A list of 8 type specimens belonging to the Subfamily Triatominaeis deposited in the Entomological Collection of the Instituto Butantan. This note reports a chronological description of the Collection including 343 specimens represented by 5 species of Panstrongylus, 18 species of Triatoma, 2 species of Psamolestes an...

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  • [Isolation of filamentous fungi from water used in a hemodialysis unit].

    abstract::Despite the relative frequency of opportunistic fungal infections among hemodialysis patients, the reservoirs for these microorganisms in the environment remain unknown, although some recent studies have made correlations with the water supply as their source. The objective of the present study was to monitor the myco...

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  • Electrocardiogram in Chagas disease.

    abstract::Since the initial descriptions of Chagas cardiomyopathy (ChCM), the electrocardiography has played a key role in patient evaluations. The diagnostic criterion of chronic ChCM is the presence of characteristic electrocardiographic (ECG) abnormalities in seropositive individuals, regardless of the presence of symptoms. ...

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  • Characterization of biofilm formation, antimicrobial resistance, and staphylococcal cassette chromosome mec analysis of methicillin resistant Staphylococcus hominis from blood cultures of children.

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  • Incidence of hepatitis C in Brazil.

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  • [Sandflies (Diptera, Psychodidae) from the transmission area for American cutaneous leishmaniasis in the town of Itupeva, the southeastern region of São Paulo state, Brazil].

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    pub_type: 杂志文章

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  • [Focus of Chagas disease in São Fidélis, in the State of Rio de Janeiro].

    abstract::Four specimens of Triatoma vitticeps and one of Panstrongylus geniculatus were caught inside a home in the municipality of São Fidélis, Rio de Janeiro. One of the specimens of Triatoma vitticeps proved positive for infection with Trypanosoma cruzi. Two people living in that home (one adult and one adolescent) presente...

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