Influence of canine brain decomposition on laboratory diagnosis of rabies.

Abstract:

:Canine brains infected with rabies virus were submitted to decomposition by being left at room temperature of 25 to 29 degrees C for up to 168 h. At 24 h intervals, brain fragments were analyzed by immunofluorescence (IF) and by the mouse intracerebral inoculation (MI) test to confirm the diagnosis of rabies and to measure the putrefaction effect on the accuracy of the diagnosis. Forty eight h after the beginning of the experiment, the MI test showed signs of impairment with four negative results, while after 72 h, 100% of the results were negative to the MI test and only one result was negative to the IF test, indicating that the threshold period for accurate diagnosis is 24 to 48 h before putrefaction. The authors recommend the shipment of suspected cases of rabies to the laboratory for confirmation, but the use of putrid materials for diagnosis is meaningless because of false-negative results.

journal_name

Rev Soc Bras Med Trop

authors

Albas A,Ferrari CI,da Silva LH,Bernardi F,Ito FH

doi

10.1590/s0037-86821999000100004

subject

Has Abstract

pub_date

1999-01-01 00:00:00

pages

19-22

issue

1

eissn

0037-8682

issn

1678-9849

journal_volume

32

pub_type

杂志文章
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    pub_type: 杂志文章,评审

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  • Coccidioidomycosis in a reference center in Northeast Brazil: clinical/epidemiological profile and most common radiological findings.

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    更新日期:1992-07-01 00:00:00

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    abstract::Benznidazole, drug of choice for Chagas disease (CD), has been associated with a high incidence of adverse reactions that can become serious, necessitating discontinuation of the drug. We describe the case of a Bolivian patient living in Spain for 9 years, who, following treatment with benznidazole for CD in indetermi...

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  • [Leptospirosis in patients with anti-HIV antibodies: report of 2 cases].

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  • Seroprevalence of hepatitis B virus infection and seroconvertion to anti-HBsAg in laboratory staff in Goiânia, Goiás.

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    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

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    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

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    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:10.1590/0037-8682-1195-2013

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  • [Concurrent leishmaniasis and human immunodeficiency virus (HIV) infection: a study of four cases].

    abstract::Few cases of concurrent leishmaniasis and HIV infection have been reported in Brazil, despite both infections being in expansion. Two cases of visceral leishmaniasis and two cases of mucocutaneous leishmaniasis are discussed. Disseminated skin and oral lesions were found in the patients with the cutaneous form of the ...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

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    authors: Borges AS,Machado AA,Ferreira MS,de Castro Figueiredo JF,Silva GF,Cimerman S,Bacha HA,Teixeira MC

    更新日期:1999-11-01 00:00:00

  • [Abdominal angiostrongyliasis: its prevention by the destruction of infecting larvae in food treated with salt, vinegar or sodium hypochlorite].

    abstract::There is a high prevalence of accidental human infection with Angiostrongylus costaricensis in some areas in southern Brazil and sometimes it presents as severe intestinal disease. Prophylaxis is important since there is no medical treatment for the disease. The ingestion of fruits and vegetables contaminated with the...

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    更新日期:1995-10-01 00:00:00

  • Behavior of Schistosoma mansoni-induced histopathological lesions in Biomphalaria glabrata submitted to ionizing radiation.

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    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

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    更新日期:2004-05-01 00:00:00

  • Occurrence of positivity for Trypanosoma cruzi in triatomine from municipalities in Southeastern Brazil, from 2002 to 2004.

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    更新日期:2010-01-01 00:00:00

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    更新日期:2017-11-01 00:00:00

  • [Growth factors in the myocardium of patients with chronic chagasic cardiomyopathy].

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  • Meningoencephalitis by Naegleria fowleri: epidemiological study in Anzoategui state, Venezuela.

    abstract::A case of primary amoebic meningoencephalitis produced by Naegleria fowleri was diagnosed in the Independencia county of Anzoategui State, Venezuela. This case motivated the realization of the present epidemiological study with the aim of identifying free-living amoebae in this area. Representative water samples were ...

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  • [Bone marrow cells transplant in the treatment of chronic chagasic cardiomyopathy].

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    pub_type: 杂志文章

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  • [HTLV-1 associated myelopathy: clinical and epidemiological profile in a 10-year case series study].

    abstract:INTRODUCTION:Human T cell lymphotropic virus type 1 (HTLV-I) myelopathy (HAM/TSP) is a progressive disabling disorder. This work aimed to analyze clinical features and epidemiology in a sample of HAM/TSP. METHODS:All HTLV-1 infected patients with diagnostic criteria for HAM/TSP, consecutively admitted to the Sarah Hos...

    journal_title:Revista da Sociedade Brasileira de Medicina Tropical

    pub_type: 杂志文章

    doi:10.1590/s0037-86822010000600013

    authors: Champs AP,Passos VM,Barreto SM,Vaz LS,Ribas JG

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  • Prevalence of cryptococcosis in Atlántico, department of Colombia assessed with an active epidemiological search.

    abstract:INTRODUCTION:Cryptococcosis is the second most frequent cause of opportunistic infections in human immunodeficiency virus (HIV) patients in Colombia. We aimed to determine the prevalence of cryptococcosis in the Colombian department of Atlántico. METHODS:An active search for cryptococcosis cases was conducted between ...

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    pub_type: 杂志文章

    doi:10.1590/0037-8682-0194-2018

    authors: Noguera MC,Escandón P,Arévalo M,García Y,Suárez LE,Castañeda E

    更新日期:2019-04-25 00:00:00

  • Disseminated cutaneous sporotrichosis in a patient with AIDS: report of a case.

    abstract::We describe a case report of disseminated cutaneous sporotrichosis as the initial presentation of AIDS in a 24-year-old HIV-positive male patient. He presented multiple ulcerated skin lesions distributed over the face, thorax, legs and arms. Biopsy of one of the cutaneous lesions was suggestive of sporotrichosis and c...

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    pub_type: 杂志文章,评审

    doi:10.1590/s0037-86822002000600018

    authors: Carvalho MT,de Castro AP,Baby C,Werner B,Filus Neto J,Queiroz-Telles F

    更新日期:2002-11-01 00:00:00