PCR-RFLP of 16S ribosomal DNA to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples.

Abstract:

INTRODUCTION:This study aimed to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples by PCR-RFLP. METHODS:Fifty-two strains identified by conventional biochemical exams were submitted to PCR amplification and digested with HinfI. Only 20 (38.5%) of the 52 strains showed a DNA pattern expected for E. gallinarum and E. casseliflavus. RESULTS:Analysis of the results of this study showed that E. gallinarum and E. casseliflavus are occasionally erroneously identified and confirmed the potential application of 16S rDNA analysis for accurate identification of these species. CONCLUSIONS:A correct identification is important to distinguish between intrinsic and acquired vancomycin resistance.

journal_name

Rev Soc Bras Med Trop

authors

Medeiros AW,d'Azevedo P,Pereira RI,Cassenego AP,Van Der Sand S,Frazzon J,Frazzon AP

doi

10.1590/s0037-86822010000100023

subject

Has Abstract

pub_date

2010-01-01 00:00:00

pages

100-1

issue

1

eissn

0037-8682

issn

1678-9849

pii

S0037-86822010000100023

journal_volume

43

pub_type

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