Comparison of in vitro activity of five antifungal agents against dermatophytes, using the agar dilution and broth microdilution methods.

Abstract:

:The purpose of this study was to compare the agar dilution and broth microdilution methods for determining the minimum inhibitory concentration (MIC) of fluconazole, itraconazole, ketoconazole, griseofulvin and terbinafine for 60 dermatophyte samples belonging to the species Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum canis. The percentage agreement between the two methods, for all the isolates with < 2 dilutions that were tested was 91.6% for ketoconazole and griseofulvin, 88.3% for itraconazole, 81.6% for terbinafine and 73.3% for fluconazole. One hundred percent agreement was obtained for Trichophyton mentagrophytes isolates evaluated with ketoconazole and griseofulvin. Thus, until a reference method for testing the in vitro susceptibility of dermatophytes is standardized, the similarity of the results between the two methods means that the agar dilution method may be useful for susceptibility testing on these filamentous fungi.

journal_name

Rev Soc Bras Med Trop

authors

Mota CR,Miranda KC,Lemos Jde A,Costa CR,Hasimoto e Souza LK,Passos XS,Meneses e Silva H,Silva Mdo R

doi

10.1590/s0037-86822009000300003

subject

Has Abstract

pub_date

2009-05-01 00:00:00

pages

250-4

issue

3

eissn

0037-8682

issn

1678-9849

pii

S0037-86822009000300003

journal_volume

42

pub_type

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