Abstract:
:A surface plasmon resonance- (SPR-) based recognition method applying H-2 Ld:Ig/peptides complexes for ex vivo monitoring cellular immune responses during murine infection with Leishmania (Leishmania) amazonensis is described. Lymphocytes from lesion-draining popliteal lymph nodes were captured on a carboxylated sensor chip surface previously functionalized with H-2 Ld:Ig (DimerX) protein bound to synthetic peptides derived from the COOH-terminal region of cysteine proteinase B of L. (L.) amazonensis. In computational analysis, these peptides presented values of kinetic constants favorable to form complexes with H-2 Ld at neutral pH, with a Gibbs free energy ΔG° < 0. The assayed DimerX:peptide complexes presented the property of attaching to distinct T lymphocytes subsets, obtained from experimentally infected BALB/c mice, in each week of infection, thus indicating a temporal variation in specific T lymphocytes populations, each directed to a different COOH-terminal region-derived peptide. The experimental design proposed herein is an innovative approach for cellular immunology studies of a neglected disease, providing a useful tool for the analysis of specific T lymphocytes subsets.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
da Silveira-Júnior LS,Souza-Silva F,Pereira BA,Cysne-Finkelstein L,Cavalcanti Júnior GB,Alves CRdoi
10.1155/2017/9089748subject
Has Abstractpub_date
2017-01-01 00:00:00pages
9089748eissn
2314-6133issn
2314-6141journal_volume
2017pub_type
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pub_type: 临床试验,杂志文章
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