Abstract：
:Certain DNA-binding proteins that regulate gene expression contain single or multiple copies of short polypeptide sequences, approximately 30 residues long, consisting of combinations of four Cys or His residues at defined spacing, so that Zn++ is complexed in tetrahedral coordination with the respective thiol-sulfur and/or imidazole-nitrogen atoms. The Zn++ ion evidently serves as a strut that stabilizes folding of the domain into a 'finger-loop', which is capable of site-specific binding to double-stranded DNA. This article reviews the evidence (a) that finger-loop domains have been highly conserved during evolution, (b) that they furnish one of the fundamental mechanisms for regulating gene expression, and (c) that a metal ion (e.g., Zn++) is required for binding of finger-loops to DNA and for their biological functions. The authors' search of amino acid sequences of 38 transforming proteins identified possible finger-loop domains in the myc, fms, fps, raf-1, rfp, src, syn, yes, erbA, int-1, and TGF-alpha gene-products. The search incidentally revealed possible finger-loop domains in human insulin receptor, which may provide a mechanistic explanation for recent observations that insulin, after binding to its cell surface receptor, is translocated to hepatocyte nuclei and becomes bound to chromatin. Zn++-coordination sites in finger-loop domains are proposed as potential targets for metal toxicity; substitution of Ni++, Co++, or Cd++ for Zn++ in finger-loops of transforming proteins is suggested as an hypothetical mechanism for metal carcinogenesis.
journal_name
Ann Clin Lab Scijournal_title
Annals of clinical and laboratory scienceauthors
Sunderman FW Jr,Barber AMsubject
Has Abstractpub_date
1988-07-01 00:00:00pages
267-88issue
4eissn
0091-7370issn
1550-8080journal_volume
18pub_type
杂志文章,评审abstract：:Toll-like receptors (TLR) activation is thought to modulate the macrophage cholesterol efflux and contribute to the atherosclerosis progression; however, the precise pathophysiological mechanism remains unclear. We investigated the effects of TLR2- and TLR4-activation on the expression of the ATP-binding cassette (ABC...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2017-08-01 00:00:00
abstract：:Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to measure 6 metabolic compounds of the adrenocorticosteroid pathway simultaneously on residual specimens from patients who had previously been previously diagnosed, on the basis of immunoassays, as having congenital adrenal hyperplasia (CAH), 11 beta-...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2001-04-01 00:00:00
abstract：:Lymphocytes from rheumatoid arthritis patients have been fused with drug-marked human myeloma cell lines and hybrids selected which secrete rheumatoid factors. Limiting dilution and soft agarose plates have been used to clone these hybrids, but so far stable clones have not been obtained. Epstein-Barr virus has been u...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:A case of a 40 year-old woman with Hodgkin's disease who developed acute non-lymphocytic leukemia (ANLL) accompanied by several abnormal chromosomal findings is illustrated. A very unusual coincidental finding was the coexistence of Hodgkin's lymphoma and ANLL in the same lymph node. ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:Neuropeptide modulation of hematopoietic stem cell differentiation was studied in an in vitro murine system. Endorphins were able to influence the erythropoietin-dependent differentiation of bone marrow cells into erythroid colony forming units in a dose dependent manner. The effects on progenitor cell maturation were...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1987-09-01 00:00:00
abstract：:Iron pill gastritis has been shown to be associated with superficial gastric erosion and deposition of iron in lamina propria and gastric antral glands. However, iron absorption in gastric parietal and chief cells is rare. We present a case of a 62-year-old man with iron deficiency anemia. His past medical history is ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2017-05-01 00:00:00
abstract：:Testing of blood for antibodies to the human immunodeficiency virus type 2 (HIV2) has begun. A major benefit of this testing will be the elimination of the exclusion of blood donors on the basis of geographic origin. Will this test be helpful? Present anti-HIV1 testing detects between 60 to 90 percent of those samples...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1992-09-01 00:00:00
abstract：:Metastatic tumors to the breast from an extramammary site are rare entities and may present diagnostic difficulties for the surgical pathologist because of frequent histological similarities to primary neoplasms in this location. A case is reported of medullary thyroid carcinoma metastatic to the breast in a 28-year-o...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1994-09-01 00:00:00
abstract：:The fragile histidine triad (FHIT) gene encompasses the most common human fragile site, FRA3B at 3p14.2, a region that is involved in homozygous deletions in a variety of human tumors. FHIT is considered to be a tumor suppressor gene that is frequently inactivated in various types of cancer. To study the role of the F...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2010-07-01 00:00:00
abstract：:To investigate the relationships of tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL), anthropometric variables, and lipid parameters, we measured serum TRAIL concentrations, body mass index (BMI), total body fat (TBF), and serum lipid profiles in 207 healthy adults. There were no significant diffe...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2004-10-01 00:00:00
abstract：:A series of studies were undertaken to evaluate the chemotherapeutic response to various antineoplastic drugs of human breast (MX-1) or colon (CX-2) tumor xenografts growing in genetically athymic (nude) mice. Fragments (2mm3) of either tumor type were implanted subcutaneously into the subaxillary region of NIH Swiss ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1978-01-01 00:00:00
abstract：:D-dimer is formed during activation of the coagulation system and is commonly assayed in order to diagnose disseminated intravascular coagulation, deep vein thrombosis, and pulmonary embolism. Enzyme-linked immunosorbent assay has been validated as the reference method, but it is a time-consuming procedure. The object...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2011-10-01 00:00:00
abstract：:The detection of oligoclonal bands of IgG in cerebrospinal fluid by isoelectric focusing is a useful aid in the diagnosis of multiple sclerosis. At present, this test is performed on polyacrylamide gels which are technically very difficult to prepare and are not suitable for the routine laboratory. A technique is desc...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1984-03-01 00:00:00
abstract：:In a prospective study, M4RT medium was compared to the traditional M4 medium to transport cervical swab specimens for Neisseria gonorrhoeae/Chlamydia trachomatis (NG/CT) PCR testing using the Roche COBAS Amplicor. Two cervical swab samples were collected from 270 consecutive patients screened for NG/CT in a satellite...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：OBJECTIVE:Intrauterine hypoxia/asphyxia is not the cause, but a consequence of different pathological conditions that requires a more detailed study of the morphogenesis of perinatal death. METHODS:Structural changes in placentas of intrauterine fetal demise (IUFD) in different stages of intrauterine period and placen...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2020-11-01 00:00:00
abstract：:The serum fructosamine concentrations measured in 64 diabetic patients correlated (r = 0.73) with glycated hemoglobins (HbA1c). However, 23 percent of the diabetic patients had normal fructosamine and abnormal HbA1c levels. In order to determine whether or not the discrepant values were the result of recent glycemic r...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
abstract：:Although serum bilirubin determination is a common procedure in most clinical laboratories, the test seems to be of particular importance in the screening of neonates, especially the premature or the erythroblastotic infant. However, bilirubin determination is subject to interferences, particularly in the presence of ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1980-11-01 00:00:00
abstract：:Pseudotumor cerebri (PTC) is characterized by increased intracranial pressure and papilledema without a mass lesion. PTC predominantly affects obese women. Currently, the pathogenesis of PTC is obscure. Since cytoskeletal abnormalities are found in many neurodegenerative diseases, we hypothesized that some cytoskeleta...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2006-04-01 00:00:00
abstract：:Human diseases caused by Haemophilus paraphrophilus (H.paraphrophilus) are unusual. The following case report describes a 67-year-old man who presented with pain and swelling of the right side of the face. Fine needle aspiration suggested a parotid gland abscess. Microbiological studies identified H.paraphrophilus. Th...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2012-10-01 00:00:00
abstract：:The case is described of a low birth weight infant who developed severe neutropenia, thrombocytopenia and monocytosis in the course of staphylococcal septicemia. Light microscopy examination of the blood revealed bacteria inside the monocytes. On electron microscopy, the monocytes were degranulated and distorted by th...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1982-11-01 00:00:00
abstract：:Three benign forms of muscle tumors and three variants of rhabdomyosarcoma are discussed from the point of view of their histology and behabior. Recent combined modality therapy has significantly improved the prognosis in at least the embryonal form of rhabdomyosarcoma. ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1975-11-01 00:00:00
abstract：:Adenoid cystic carcinoma (ACC) is a rare cervical malignancy, and its coexistence with other malignancies in the uterine cervix is extremely rare. Here, we present a case of coexistence of ACC and invasive squamous cell carcinoma (SCC) of the uterine cervix, as well as a literature review. A 68-year-old Chinese woman ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章,评审
doi：
更新日期：2014-10-01 00:00:00
abstract：:This study compared the assay results of HBV markers obtained with two automated immunoassay systems in serum samples with concomitant HBV antigens and antibodies. Eighty-nine samples of concomitant HBsAg and anti-HBs and 74 samples of concomitant HBeAg and anti-HBe were analyzed by the Abbott Architect i2000 and the ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2010-01-01 00:00:00
abstract：:To investigate the relationships of trace element concentrations vs hematopoiesis and iron parameters, we measured lead, copper, zinc, cadmium, and ferritin levels in 251 healthy adolescents. Concentrations of trace metals were determined by atomic absorption spectrophotometry. There were no significant gender-related...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2005-10-01 00:00:00
abstract：:Patent ductus arteriosus (PDA) is highly prevalent in pre-term neonates (PTN) and has been recognized as a neonatal co-morbidity. The purpose of this study was to determine if levels of brain (or B-type) natriuretic peptide (BNP), a peptide secreted by ventricular myocytes in response to volume or pressure overload, c...
journal_title：Annals of clinical and laboratory science
pub_type： 临床试验,杂志文章
doi：
更新日期：2011-04-01 00:00:00
abstract：:A case of multiple myeloma producing IgD-M component with light chains of lambda type is reported. It involved the skeleton and was complicated by compression of the spinal cord and massive extension to the pleura. The urine contained lambda Bence Jones protein. Under the electron microscope, the neoplastic plasma cel...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1978-05-01 00:00:00
abstract：:A considerable variabilility in the effects of sample handling on NK cytotoxicity has been observed. Using flow cytometry, NK cytotoxicity assays and lymphocyte subset analysis of Ficoll-Hypaque-separated peripheral blood mononuclear cells (PBMCs) isolated from whole blood stored under various conditions were performe...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2013-07-01 00:00:00
abstract：:Experimental evidence documenting the formation of a relatively stable V(IV) species appears to be important with regard to the biochemical mechanism of reduction of vanadate by enzymatic systems. The present study demonstrates that a mixture of vanadate and glutathione reductase/nicotinamide-adenine-dinucleotide phos...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1997-11-01 00:00:00
abstract：:This study assessed the progression of renal damage in obese Zucker rats in response to deoxycorticosterone acetate (DOCA)-salt-induced hypertension. Renal damage was evaluated by light microscopy and urine analysis at weekly intervals during the developmental phase of DOCA-salt hypertension and once during the platea...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:We previously reported that proper dilution of sera that contain IgG monoclonal proteins (M-proteins) is necessary for accurate quantitation of serum albumin and M-protein concentrations separated by serum protein electrophoresis (SPE) using the Beckman PARAGON agarose electrophoresis system. We now report the signifi...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2003-04-01 00:00:00
