Abstract:
:Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to measure 6 metabolic compounds of the adrenocorticosteroid pathway simultaneously on residual specimens from patients who had previously been previously diagnosed, on the basis of immunoassays, as having congenital adrenal hyperplasia (CAH), 11 beta-hydroxylase deficiency, 21-hydroxylase deficiency, or Addison disease (adrenal insufficiency). Two subjects with normal adrenal function had serum cortisol values of 13.6 and 8.9 micrograms/dL and serum cortisone values of 2.1 and 0.6 microgram/dL, but the rest of the compounds were undetectable. Two patients with 11 beta-hydroxylase deficiency had serum 11 beta-deoxycortisol values of 14.9 and 10.0 micrograms/dL and serum 11-deoxycorticosterone values of 3.9 and 1.0 microgram/dL, but their serum levels of cortisol and cortisone were diminished. A patient with 21-hydroxylase deficiency had a highly increased serum 17-hydroxyprogesterone concentration of 28.5 micrograms/dL (or 28,500 ng/dL, the traditional unit to report this assay) and a serum 21-deoxycortisol concentration of 6.9 ug/dL (this is a pathologic marker of 21-hydroxylase deficiency that is nondetectable in sera of healthy subjects). This patient also had diminished concentrations of serum cortisol and cortisone (0.9 and 0.3 microgram/dL, respectively). At 30 and 60 min after corticotropin (ACTH) stimulation, serum cortisol was the only compound that showed a dramatic increase in the normal subjects; the patient with 21-hydroxylase deficiency showed an increase of serum 17-hydroxyprogesterone level, but no increase of serum cortisol level; the patient with Addison disease showed no increase in the levels of serum cortisol or other compounds. Metyprapone, which blocks 11 beta-hydroxylase activity, increased the serum 11-deoxycorticosteroid levels and decreased the serum cortisol level. This pilot study demonstrates that it is feasible to use LC-MS/MS for the laboratory diagnosis of adrenal cortical dysfunction. The authors envision that LC-MS/MS may soon become an ideal analytical technique for the diagnosis of such endocrine diseases.
journal_name
Ann Clin Lab Scijournal_title
Annals of clinical and laboratory scienceauthors
Kao PC,Machacek DA,Magera MJ,Lacey JM,Rinaldo Psubject
Has Abstractpub_date
2001-04-01 00:00:00pages
199-204issue
2eissn
0091-7370issn
1550-8080journal_volume
31pub_type
杂志文章abstract::The purpose of this study was to assess whether the LG Anti-HIV 1/2 Plus ELISA (LG Life Sciences, Seoul, Korea), a new third-generation enzyme-linked immunosorbent assay for the detection of HIV infection, has improved sensitivity and specificity in comparison to the other licensed third-generation assays. The sensiti...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::Hydroxyethyl starch (HES) has been shown to be beneficial in several inflammatory situations, but the mechanisms are unclear. The present study tested the hypothesis that HES has effects on nuclear factor kappa B (NF-kappaB) activation and the expression of inflammatory mediators induced by lipopolysaccharide. Sepsis ...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2003-10-01 00:00:00
abstract::Severe spermatogenic alterations occur in association with diabetic manifestations in the nonobese diabetic (NOD) mouse. A study was undertaken to determine whether or not administration of insulin during initial appearance of diabetic changes could inhibit the interference with spermatogenesis. Male NOD mice injected...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1995-05-01 00:00:00
abstract:BACKGROUND:We compared the AdvanSure hepatitis B virus real-time polymerase chain reaction (AdvanSure HBV) kit with three other HBV DNA quantification assays and evaluated its performance. METHODS:The AdvanSure HBV real-time PCR assay was compared with the Abbott RealTime HBV Quantification Kit, the COBAS TaqMan HBV T...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
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更新日期:2013-04-01 00:00:00
abstract:OBJECTIVE:Intrauterine hypoxia/asphyxia is not the cause, but a consequence of different pathological conditions that requires a more detailed study of the morphogenesis of perinatal death. METHODS:Structural changes in placentas of intrauterine fetal demise (IUFD) in different stages of intrauterine period and placen...
journal_title:Annals of clinical and laboratory science
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abstract::Patients with heterozygous beta-thalassemia minor have a decreased hematocrit (HCT). Since the HCT is a primary determinant of whole blood viscosity, the known reduction in HCT in beta-thalassemia minor should lead to a measurable reduction of whole blood viscosity. The influence of the relatively lower mean corpuscul...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1992-07-01 00:00:00
abstract::A case of a 40 year-old woman with Hodgkin's disease who developed acute non-lymphocytic leukemia (ANLL) accompanied by several abnormal chromosomal findings is illustrated. A very unusual coincidental finding was the coexistence of Hodgkin's lymphoma and ANLL in the same lymph node. ...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract::A procedure for the measurement of free erythrocyte protoporphyrin (FEP) in a drop of blood collected on filter paper is described. The method is useful as a screening test for lead poisoning in children. Based on the FEP finding and blood lead tests, asymptomatic children are classified into four major categories. A ...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1976-05-01 00:00:00
abstract::Secretion of nickel into unstimulated and stimulated saliva of rabbits was studied after a single intraperitoneal injection of nickel chloride labelled with 63Ni. Maximum 63Ni in unstimulated saliva was obtained in specimens collected one hr after maximum serum-63Ni was reached and two hrs after the nickel administrat...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1979-11-01 00:00:00
abstract::A rapid homogeneous enzyme immunoassay for theophylline has been adapted to a centrifugal analyzer. The sample volume used was 10 microliter and sera from as many as 28 patients could be measured in 180 seconds. The assay temperature was 30 degrees and the absorbance rates were measured at 340 nm. Within-run and run-t...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1980-07-01 00:00:00
abstract::This is a review of the following human gene products which appear in the prenatal period, subsequently are repressed, and reappear in malignant neoplasms: alpha fetal protein; carcinoembryonic antigen; Regan's isoenzyme; fetal sulfoglycoprotein; a fetal liver ferroprotein; beta-S-fetoprotein; gamma-fetoprotein; carci...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1978-09-01 00:00:00
abstract:OBJECTIVE:This study aims to investigate the effect of different concentrations of docosahexaenoic acid (DHA) on proliferation and apoptosis in HepG2 cell lines, and to research the possible molecular mechanisms. METHODS:DHA concentration was 0 g/mL in the negative control group, and 15, 30, 45, 60 and 75 ug/mL, respe...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2019-01-01 00:00:00
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journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2018-07-01 00:00:00
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journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2015-10-01 00:00:00
abstract::Although serum bilirubin determination is a common procedure in most clinical laboratories, the test seems to be of particular importance in the screening of neonates, especially the premature or the erythroblastotic infant. However, bilirubin determination is subject to interferences, particularly in the presence of ...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1980-11-01 00:00:00
abstract::We examined the results of 1.3 million drug tests performed on patients being monitored for compliance with pain medications and substance abuse rehabilitation to determine if the 2016 CDC prescribing guidelines had any impact on opiate benzodiazepine use. We observed that the combination of the opiate drugs morphine,...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2020-05-01 00:00:00
abstract::One hundred thirty-three Streptococcus milleri group (S. anginosus) isolates were recovered from 487 surgical patients. The streptococci were recovered from 33 percent of intra-abdominal infection cultures (84/257). 22 percent of samples from penetrating visceral trauma (19/86), 52 percent of perirectal abscess specim...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::The most common ultrastructural features of endocrine tumors are reviewed. The diagnostic applications of the electron microscope are illustrated by citation of case histories. ...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1979-03-01 00:00:00
abstract::In vitro determination of specific IgE antibodies in serum is the most frequently used method, besides the skin test, for diagnosing allergies. Standardized and reproducible assays of specific IgE antibodies contribute to the quality of diagnosis and treatment of allergic disease. This study compared the results and p...
journal_title:Annals of clinical and laboratory science
pub_type: 临床试验,杂志文章
doi:
更新日期:2005-07-01 00:00:00
abstract::A series of studies were undertaken to evaluate the chemotherapeutic response to various antineoplastic drugs of human breast (MX-1) or colon (CX-2) tumor xenografts growing in genetically athymic (nude) mice. Fragments (2mm3) of either tumor type were implanted subcutaneously into the subaxillary region of NIH Swiss ...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract::To define further the nature of the decreased responsiveness of neonatal neutrophils to chemotactic factor stimulation, neutrophil shape change induced by various concentrations of N-formyl-methionyl-leucyl-phenyl-alanine (fMLP) was studied using a whole blood assay. Samples from 48 full term neonates and paired healt...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1985-11-01 00:00:00
abstract::The microbiome has recently become a key interest for cancer research. Anti-tumor effects of reinforced clostridium media (RCM) were investigated for all ingredients of RCM, which showed that yeast extract could be a candidate for this phenomenon. MTT assay, cell counting, cell death analysis, cell cycle analysis, and...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2020-01-01 00:00:00
abstract::While there is no single best procedure for performing prenatal diagnosis, ther is a rationale or strategy which will produce correct, reliable results. The investigator should be experienced with all the tests and know what to expect from each. At least two of these tests should e used (more if there s ambiguity) on ...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章,评审
doi:
更新日期:1982-09-01 00:00:00
abstract::Hemoglobin H (Hb H) disease is usually characterized by the existence of Hb H, which influences the degree of functional anemia. We here report a patient with a rare Hb H disease genotype (-SEA/-α27.6), who was observed to paradoxically have no detectable Hb H fraction on electrophoresis. To date, the reason why the q...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
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更新日期:2018-11-01 00:00:00
abstract::Point-of-care testing (POCT) has been used for illicit substance screening in several settings, primarily in law enforcement and drug treatment centers. In this study, we evaluate the use of this screening approach in the emergency department (ED) of a tertiary-care, urban medical center. Aliquots of urine specimens w...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2002-10-01 00:00:00
abstract::Widespread drug abuse, a comparatively recent medicosocial phenomenon, presents protean clinical patterns and challenging diagnostic problems daily that mimic classical medical syndromes. However, few reports have delineated predominantly neurologic syndromes associated with drug abuse. Five patients were observed ill...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1976-11-01 00:00:00
abstract::Hematological malignancies exhibit many clinical presentations, from ambiguous systemic symptoms to rapid multi-organ failure. By identifying common clinical, laboratory, and autopsy findings in patients with a hematological malignancy, living patients may be diagnosed and treated earlier. We retrospectively reviewed ...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
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更新日期:2015-10-01 00:00:00
abstract::Thirty-two Enterobacter cloacae strains that are less susceptible to cefepime than to ceftazidime were collected. This unique phenotype of 8 strains was confirmed using the agar dilution method. OXA1, OXA10, OXA31 and OXA35 were detected in 3, 2, 3, and 2 strains, respectively, whereas all strains were negative for PS...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
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更新日期:2018-05-01 00:00:00
abstract::In this study, we investigated the relationship between thyroid function and serum uric acid (UA) levels in subjects without overt thyroid dysfunction. A total of 6,085 patients were included in this cross-sectional study. Free thyroxine (FT4) levels were positively and linearly associated with serum UA levels (p<0.00...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2015-01-01 00:00:00
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journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
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更新日期:2016-09-01 00:00:00