Abstract：
BACKGROUND:We compared the AdvanSure hepatitis B virus real-time polymerase chain reaction (AdvanSure HBV) kit with three other HBV DNA quantification assays and evaluated its performance. METHODS:The AdvanSure HBV real-time PCR assay was compared with the Abbott RealTime HBV Quantification Kit, the COBAS TaqMan HBV Test, and the VERSANT HBV branched DNA 3.0 assay. The precision, linearity, accuracy, limit of detection (LOD), cross reactivity, and genotype inclusivity of the assays were compared, and any influence of the sampling tube type was evaluated. RESULTS:The AdvanSure HBV PCR showed good correlations with the three other HBV DNA assays. The R(2) coefficients were 0.944, 0.939, and 0.921 with the Abbott RealTime HBV Quantification Kit, the COBAS TaqMan HBV Test, and the VERSANT bDNA 3.0 assay, respectively. Linearity was good in the tested range of 1.15-8.45 log10 IU/ml. The lower LOD result was consistent with the 18 IU/ml claimed by the manufacturer. HBV genotypes A-F were all correctly amplified, and no cross reactivity was found in samples with high HCV RNA levels or high protein concentrations. The results were not influenced by the sample preparation tube (i.e. plain tube, SST, and EDTA containing tubes). CONCLUSION:The AdvanSure HBV real-time PCR assay is a reliable method for quantifying HBV DNA levels in routine laboratory testing.
journal_name
Ann Clin Lab Scijournal_title
Annals of clinical and laboratory scienceauthors
Kim H,Shin S,Oh EJ,Kahng J,Kim Y,Lee HK,Kwon HJsubject
Has Abstractpub_date
2013-04-01 00:00:00pages
230-7issue
2eissn
0091-7370issn
1550-8080pii
43/2/230journal_volume
43pub_type
杂志文章abstract：:Widespread drug abuse, a comparatively recent medicosocial phenomenon, presents protean clinical patterns and challenging diagnostic problems daily that mimic classical medical syndromes. However, few reports have delineated predominantly neurologic syndromes associated with drug abuse. Five patients were observed ill...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1976-11-01 00:00:00
abstract：:Hand, foot and mouth disease (HFMD) is a serious public health problem. Generally, it is considered that HFMD is mainly caused by enterovirus 71 (EV71) and coxsackievirus A16 (CVA16). Nevertheless, the incidence of HFMD caused by coxsackievirus A6 (CVA6) and coxsackievirus A10 (CVA10) has increased significantly and C...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2017-03-01 00:00:00
abstract：:People of the Jehovah's Witness faith believe that they shall "abstain from blood." Because of this belief, we encounter the challenges from Jehovah's Witness patients who actively seek medical care for themselves and their children, but refuse the transfusion of blood products, which may result in increased morbidity...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2016-12-01 00:00:00
abstract：:We present an adult autopsy case of primary hyperoxaluria type 1. Diagnosis was established with skin biopsy and subsequent genetic analysis one month prior to death. At autopsy, calcium oxalate crystals refringent to polarized light were found systemically. Interestingly, however, calcium oxalate crystals were not id...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2013-07-01 00:00:00
abstract：:We report a case of a 69-yr-old previously healthy man with acute spontaneous Achilles tendon rupture and severe tendonitis, which occurred after 2 weeks of steroid therapy for newly diagnosed giant cell arteritis. The Achilles tendon rupture was treated conservatively and the tendonitis resolved incrementally with st...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2003-07-01 00:00:00
abstract：:Intravascular large B-cell lymphoma (IVLBCL) is a rare variant of extranodal diffuse large Bcell lymphoma with only a few more than 300 cases reported. It is characterized as lymphoma cells confined to the lumina of small vessels, so patients usually do not present with masses or lymphadenopathy. Clinical presentation...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章,评审
doi：
更新日期：2011-07-01 00:00:00
abstract：:Chemokines are low-molecular-weight chemotactic proteins that regulate the trafficking of leukocytes to inflammatory sites and may recruit inflammatory cells to the epidermis. Chemokines are produced by many immune cells such as macrophages, mast cells, T lymphocytes and others, in response to pro-inflammatory stimuli...
journal_title：Annals of clinical and laboratory science
pub_type： 信件
doi：
更新日期：2015-07-01 00:00:00
abstract：BACKGROUND:For viral load (VL) analysis of hepatitis C virus (HCV) by Real Time PCR, approved collection tubes were lavender top (LTT) and plasma processing (PPT) tubes, that differ in that PPTs include plasma separator. Using a Real Time PCR method, we investigated how the results correlated for the two tube types. M...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2018-05-01 00:00:00
abstract：:Alterations in ion transport have been implicated in the pathogenesis of several disease states. Methods of studying ion transport, however, are rather tedious, time consuming and not well defined in terms of kinetics and reproducibility. This paper describes a rapid, simple method of examining ion transport in intact...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1981-03-01 00:00:00
abstract：:Excessive production of hydroxyl radicals in blood and liver has previously been demonstrated by us in rats with obstructive jaundice induced by common bile duct ligation (CBDL). In this study, we demonstrate overproduction of superoxide radicals in circulating blood of CBDL rats by the lucigenin-amplified chemilumine...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2001-10-01 00:00:00
abstract：:Twenty-one patients with Parkinson's disease and motor fluctuations who completed a double-blind study comparing controlled-release carbidopa/levodopa (Sinemet CR4) with standard Sinemet (SS) were evaluated one year following completion of the study. Five patients remained on CR4 alone; 16 continued on CR4 plus SS, an...
journal_title：Annals of clinical and laboratory science
pub_type： 临床试验,杂志文章
doi：
更新日期：1989-11-01 00:00:00
abstract：:Mosaic variegated aneuploidy (MVA) is a recessive condition characterized by mosaic aneuploidies, predominantly trisomies and monosomies, involving multiple chromosomes and tissues. The phenotype of MVA syndrome includes severe microcephaly and growth deficiency, central nervous system anomalies, mental retardation, m...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章,评审
doi：
更新日期：2015-01-01 00:00:00
abstract：:Inflammation plays an important role in the pathogenesis of secondary damage after spinal cord injury (SCI). The present study explored the effect of sulforaphane (SFN), a potent anti-inflammatory extract of cruciferous vegetables, on the expression of two inflammatory mediators, metalloproteinase (MMP)-9 and TNF-α, i...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2010-10-01 00:00:00
abstract：:Urine p-aminophenol (PAP) concentration serves as a biological marker for occupational exposures to aniline. We report the development of a rapid, simple spectrophotometric method for quantification of urine PAP concentration using a chemical autoanalyzer (Olympus Reply). The method involves oxidative coupling of PAP ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2004-07-01 00:00:00
abstract：:To evaluate the screening power of the fecal cytokeratin-19 test (CK-19) and the fecal occult blood test (FOBT), we performed rapid fecal CK-19 and FOBT tests on 515 stool samples from patients with various GI diseases and 814 stool samples from control patients. The rapid fecal CK-19 test (developed by DiNonA Researc...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2006-07-01 00:00:00
abstract：:Absorbed lead continuously adds to the lead deposited in the vascularized circumpulpal dentin of the teeth. Thus, this dental tissue is expected to contain a lead concentration which reflects the integrated lead exposure during the time from completion of tooth formation to tooth extraction or shedding. A method has b...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1984-07-01 00:00:00
abstract：:Numerous drugs and chemicals affect the function of human blood platelets. The mechanism of action of some medications is partly understood. Aspirin is the most frequently involved drug. It appears to interfere with the platelet release reaction by acetylation of a platelet membrane protein which may be involved in th...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章,评审
doi：
更新日期：1977-01-01 00:00:00
abstract：:To investigate the relationships of trace element concentrations vs hematopoiesis and iron parameters, we measured lead, copper, zinc, cadmium, and ferritin levels in 251 healthy adolescents. Concentrations of trace metals were determined by atomic absorption spectrophotometry. There were no significant gender-related...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2005-10-01 00:00:00
abstract：OBJECTIVE:The microscopic and chemical analysis of urine is essential for the diagnosis of patients with urinary tract infections (UTI). Quantitative urine culture is the 'gold standard' method for the diagnosis of UTI, but it is labour-intensive and time consuming. The DongJiu 8602 is a new automated urinalysis system...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2015-10-01 00:00:00
abstract：:At the cellular level, mutations in deoxyribonucleic acid (DNA) can lead to synthesis of altered proteins which are unable to sustain specific cell functions, eventually leading to its death. Veritably apoptosis, or programmed cell death, is a device to eliminate heavily mutated cells. Cell membranes with altered prot...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章,评审
doi：
更新日期：1996-01-01 00:00:00
abstract：:A quality control program of analyses of toxic metals in urine has been carried out in the Nordic countries since 1978. In connection with these programs, the advantages and disadvantages of lyophilized compared to natural urine specimens as control materials have been investigated in three successive similar studies....
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1987-05-01 00:00:00
abstract：:Malignant tumours of nonendocrine tissues may produce ectopic hormones. The most likely mechanism is depression of genes which code for hormones. Ectopic hormones are invariably peptides, and each is identical to some peptide product of an endocrine gland. However, the majority of ectopic hormones occur as biologicall...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1979-07-01 00:00:00
abstract：:HbA1 is the result of the non-enzymatic glycosylation of HbA. HbA1 reflects the integrated blood glucose level that prevailed over a period of weeks; it thus has clinical application in the assessment of the long-term blood glucose control. This ability to determine easily the long-term blood glucose should enable the...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章,评审
doi：
更新日期：1980-03-01 00:00:00
abstract：:The aim of this study was to evaluate the influence of gender differences on triglyceride (TG) response after a fatty meal in clinically defined heterozygous (h) patients with familial hypercholesterolemia (FH). Nineteen hFH men were age-matched with an equal number of premenopausal women. Plasma TG was measured befor...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2007-10-01 00:00:00
abstract：:Erythropoietin (EPO), known for its role in stimulating erythropoiesis, has recently been shown to have a cardio-protective effect in animal models of myocardial ischemia-reperfusion (I-R) injury. The mechanism of the cardio-protective effect of EPO is unclear. Part of the mechanism for EPO-induced cardio-protection m...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2005-04-01 00:00:00
abstract：:Variations in the level of prealbumin in serum and spinal fluid were noted on routine electrophoresis. Low serum levels were associated with infection, liver disorders, and malignant disease, whereas high levels were seen in inflammatory disorders. A high spinal fluid prealbumin was noted in a young child with a disse...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1987-09-01 00:00:00
abstract：:Intraventricular hemorrhage (IVH) and classical periventricular leukomalacia complex are considered the two most common forms of perinatal anoxic-ischemic brain injury. However, recently, a third entity, the periventricular leukomalacia complex (PLC) was described and was seen in 31 percent of 61 premature infants com...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1985-03-01 00:00:00
abstract：BACKGROUND:Nuclear factor-kappaB (NF-kappaB) is synthesized in the cytoplasm, complexed with its inhibitor, I-kappaB, and NF-kappaB is released in an activated (phosphorylated) form following phosphorylation of I-kappaB and proteasomal degradation of the NF-kappaB.p-kappaB complex. The free p-NF-kappaB can then be tran...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2005-07-01 00:00:00
abstract：:Lymphocytes from rheumatoid arthritis patients have been fused with drug-marked human myeloma cell lines and hybrids selected which secrete rheumatoid factors. Limiting dilution and soft agarose plates have been used to clone these hybrids, but so far stable clones have not been obtained. Epstein-Barr virus has been u...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:Utilizing fetal electrocardiography, ultrasound, M-mode and two dimensional echocardiography, fetal cardiac function, structure, and rhythm were studied in pregnancies with obstetrical risk factors. Risk factors for fetal cardiac abnormalities were defined to include: maternal diabetes, connective tissue disorders; Rh...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1982-03-01 00:00:00
