Abstract：
:Excessive production of hydroxyl radicals in blood and liver has previously been demonstrated by us in rats with obstructive jaundice induced by common bile duct ligation (CBDL). In this study, we demonstrate overproduction of superoxide radicals in circulating blood of CBDL rats by the lucigenin-amplified chemiluminescence technique. To pinpoint the molecular agents that mediate these processes, we measured circulating proinflammatory cytokines, such as tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta ( IL-1beta), and interleukin-6 (IL-6) in controls and CBDL rats. Concentrations of these cytokines in blood of CBDL rats were markedly elevated when compared to the controls (TNF-alpha: 36.7 +/- 5.0 vs 13.8 +/- 0.5 pg/mL; IL-6: 2,814 +/- 1,740 vs 0 pg/mL; IL-1beta: 11.9 +/- 2.6 vs 0 pg/mL). The overproduction of free radicals triggered by elevated cytokines in CBDL rats was correlated with the activation of NF-kappaB in hepatic tissue. Using the TdT-mediated dUTP nick-end label staining technique, we showed that hepatic tissue sections from CBDL rats had an increase in the apoptotic index (AI). Based on these findings, we propose that the severe hepatic injury in CBDL rats is mediated by a cycle that involves the activation of NF-kappaB by combined action of proinflammatory cytokines and reactive oxygen species (ROS). NF-KB, in turn, initiates the transcription of cytokine genes (eg, IL-6, IL-8, TNF-alpha), which triggers hepatic injury, at least in part, by a free radical-mediated apoptotic mechanism. Elevated ROS may be as a positive-feedback signal that triggers NF-KB reactivation; the severe hepatic injury of CBDL rats may result from perpetuation of this vicious cycle.
journal_name
Ann Clin Lab Scijournal_title
Annals of clinical and laboratory scienceauthors
Liu TZ,Lee KT,Chern CL,Cheng JT,Stern A,Tsai LYsubject
Has Abstractpub_date
2001-10-01 00:00:00pages
383-90issue
4eissn
0091-7370issn
1550-8080journal_volume
31pub_type
杂志文章abstract：:Histologic sections of thyroid glands resected from 12 patients with Hashimoto's thyroiditis have been studied in which areas were present showing clear nuclear changes such as those seen in papillary carcinoma. The patients' ages ranged from 28 to 78 years (mean = 57.3); 11 were women and one was a man. The lesions p...
journal_title：Annals of clinical and laboratory science
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journal_title：Annals of clinical and laboratory science
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journal_title：Annals of clinical and laboratory science
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更新日期：2016-07-01 00:00:00
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journal_title：Annals of clinical and laboratory science
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journal_title：Annals of clinical and laboratory science
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
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journal_title：Annals of clinical and laboratory science
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journal_title：Annals of clinical and laboratory science
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journal_title：Annals of clinical and laboratory science
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更新日期：1982-03-01 00:00:00
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journal_title：Annals of clinical and laboratory science
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journal_title：Annals of clinical and laboratory science
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journal_title：Annals of clinical and laboratory science
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journal_title：Annals of clinical and laboratory science
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