Abstract：
:In vitro determination of allergen-specific IgE (sIgE) represents an important aid in the diagnosis and treatment of allergy. Improvements in laboratory methodology--the development of second, and now third generation assays for sIgE--have brought about major advances in speed, convenience, performance, and in the standards for judging performance. In this study, following the NCCLS I/LA20-A guidelines, we evaluated the analytical performance of a quantitative chemiluminescent enzyme immunoassay for sIgE using the continuous random access IMMULITE 2000 system. Defining features of this "third generation" sIgE assay include a true zero calibrator with a detection limit and functional sensitivity of 0.1 and 0.2 kU/L, respectively. The use of liquid allergens allows for complete automation, fast binding kinetics between IgE and the natural allergenic protein conformations, and a time-to-first-result of 65 min. Stable reagents and the low nonspecific signal associated with the liquid allergens and centrifugal wash technique permit extension of the measuring range to 0.1-100 kU/L, based on lot-specific, factory-calibrated master curves standardized to the WHO 75/502 reference standard. The assay demonstrated good precision and linearity over its measuring range. Relative to a first generation RIA (mRAST, from Hycor), clinical sensitivity, specificity, and concordance were 88%, 92%, and 90%, respectively (n = 812). Quantitative comparisons to a second generation assay yielded a linear regression relationship of IMMULITE 2000 = 0.99 (Pharmacia FEIA) + 1.99 kU/L, r = 0.859 (n = 169).
journal_name
Ann Clin Lab Scijournal_title
Annals of clinical and laboratory scienceauthors
Li TM,Chuang T,Tse S,Hovanec-Burns D,El Shami ASsubject
Has Abstractpub_date
2004-01-01 00:00:00pages
67-74issue
1eissn
0091-7370issn
1550-8080journal_volume
34pub_type
杂志文章abstract：:We previously reported that proper dilution of sera that contain IgG monoclonal proteins (M-proteins) is necessary for accurate quantitation of serum albumin and M-protein concentrations separated by serum protein electrophoresis (SPE) using the Beckman PARAGON agarose electrophoresis system. We now report the signifi...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2003-04-01 00:00:00
abstract：BACKGROUND:Mean platelet volume (MPV) is an inexpensive, routinely measured clinical parameter, and its elevation shows an increase in the size and activity of platelets. It has been shown that MPV can be used as a prognostic biomarker in certain chronic diseases. In the present study, MPV in patients with hepatitis B ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
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更新日期：2018-09-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
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更新日期：2004-04-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
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journal_title：Annals of clinical and laboratory science
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journal_title：Annals of clinical and laboratory science
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更新日期：2002-01-01 00:00:00
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journal_title：Annals of clinical and laboratory science
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
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更新日期：1978-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：2009-10-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1984-03-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章,评审
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更新日期：1999-07-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
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更新日期：1989-03-01 00:00:00
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journal_title：Annals of clinical and laboratory science
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journal_title：Annals of clinical and laboratory science
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更新日期：1984-01-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1987-09-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2015-01-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
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更新日期：1985-09-01 00:00:00
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journal_title：Annals of clinical and laboratory science
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更新日期：2005-04-01 00:00:00
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journal_title：Annals of clinical and laboratory science
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journal_title：Annals of clinical and laboratory science
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更新日期：2012-01-01 00:00:00
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journal_title：Annals of clinical and laboratory science
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更新日期：1994-09-01 00:00:00
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journal_title：Annals of clinical and laboratory science
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更新日期：1996-09-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
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更新日期：1981-01-01 00:00:00
abstract：BACKGROUND:Various assays for detecting high-risk human papillomavirus (HR HPV) have been introduced recently, including the Abbott RealTime High-Risk HPV assay. We sought to compare the performance of Abbott PCR to Hybrid Capture 2 for the detection of HR HPV. METHODS:A total of 941 cervical swab specimens were obtai...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2016-09-01 00:00:00
