Abstract：
BACKGROUND:Various assays for detecting high-risk human papillomavirus (HR HPV) have been introduced recently, including the Abbott RealTime High-Risk HPV assay. We sought to compare the performance of Abbott PCR to Hybrid Capture 2 for the detection of HR HPV. METHODS:A total of 941 cervical swab specimens were obtained. We submitted all specimens for HR HPV detection with HC2 and Abbott PCR, and then additionally analyzed discordant and concordant positive results using restriction fragment mass polymorphism (RFMP) genotyping analysis. RESULTS:HC2 detected one of 13 HR HPV types in 12.3% (116/941) of cases, while Abbott PCR detected one of 14 detectable HR HPV types in 12.9% (121/941) of cases. The overall agreement rate was 97.3% with a kappa coefficient of 0.879. Discordant results between these two assays were observed in 25 cases. HC2 showed a sensitivity of 90.0% and specificity of 95.9%, while Abbott PCR showed a sensitivity of 98.0% and specificity of 96.8% when using RFMP results as the gold standard. For HPV 16/18 detection, Abbott PCR showed 95.8%/88.9% sensitivity and 99.2%/99.8% specificity, respectively. The overall coinfection rate between HPV 16, 18 and non-16/18 was 9.9% (12/121) in Abbott PCR analysis. CONCLUSIONS:Considering its high agreement rate with HC2, higher sensitivity/specificity compared to HC2, and ability to differentiate HPV 16/18 from other HPV types, Abbott PCR could be a reliable laboratory testing method for the screening of HPV infections.
journal_name
Ann Clin Lab Scijournal_title
Annals of clinical and laboratory scienceauthors
Ko K,Yu S,Lee EH,Park H,Woo HY,Kwon MJsubject
Has Abstractpub_date
2016-09-01 00:00:00pages
522-8issue
5eissn
0091-7370issn
1550-8080pii
46/5/522journal_volume
46pub_type
杂志文章abstract：:A high performance liquid chromatography has been adapted to the measurement of putrescine, spermidine and spermine in plasma and erythrocytes. Detection was by fluorimetry using o-phthalaldehyde which formed fluorophores with the polyamines. Sensitivity of the method was better than 10 pmol of each polyamine, and rec...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1980-03-01 00:00:00
abstract：:Methodologies for T and B lymphocyte quantitation, lymphocyte blast transformation (LBT) and carbohydrate (CHO) metabolism are important for assessing host lymphocyte response in the clinical laboratory. Modifications of methods for each of these techniques are presented. Results from studies of normal ambulatory adul...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1980-11-01 00:00:00
abstract：:Absorbed lead continuously adds to the lead deposited in the vascularized circumpulpal dentin of the teeth. Thus, this dental tissue is expected to contain a lead concentration which reflects the integrated lead exposure during the time from completion of tooth formation to tooth extraction or shedding. A method has b...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1984-07-01 00:00:00
abstract：:A rapid homogeneous enzyme immunoassay for theophylline has been adapted to a centrifugal analyzer. The sample volume used was 10 microliter and sera from as many as 28 patients could be measured in 180 seconds. The assay temperature was 30 degrees and the absorbance rates were measured at 340 nm. Within-run and run-t...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1980-07-01 00:00:00
abstract：BACKGROUND:For viral load (VL) analysis of hepatitis C virus (HCV) by Real Time PCR, approved collection tubes were lavender top (LTT) and plasma processing (PPT) tubes, that differ in that PPTs include plasma separator. Using a Real Time PCR method, we investigated how the results correlated for the two tube types. M...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2018-05-01 00:00:00
abstract：:An unusual case is reported of pleomorphic large cell sarcoma of the spleen with rhabdomyosarcomatous differentiation in a 34-year old male. According to our knowledge, such a neoplasm has never been reported in the literature. ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1999-10-01 00:00:00
abstract：:Oxidative stress plays a pivotal role in the pathogenesis of multiple sclerosis (MS). Inactivating polymorphism of genes encoding detoxification enzymes, such as NQO1 and GSTP1 could influence susceptibility to MS. The monoclonal antibody natalizumab is an effective treatment in MS. Natalizumab's efficacy in MS patien...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2016-12-01 00:00:00
abstract：BACKGROUND:Quality assurance of newly developed or manufactured blood grouping reagents with reagent red blood cells (RBCs) is crucial in the process of product approval by governmental agency. However, RBCs with rare blood group are not easily available in the fresh state. We investigated the feasibility of cryopreser...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2018-03-01 00:00:00
abstract：:Recent improvement in imaging technology has enabled ultrasound to assume an increasingly vital role in the identification and evaluation of renal disease. Renal masses can be differentiated. The nonfunctioning kidney can be imaged and obstruction or inflammation diagnosed. When further information is needed, an ultra...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章,评审
doi：
更新日期：1981-07-01 00:00:00
abstract：:Erythropoietin (EPO), known for its role in stimulating erythropoiesis, has recently been shown to have a cardio-protective effect in animal models of myocardial ischemia-reperfusion (I-R) injury. The mechanism of the cardio-protective effect of EPO is unclear. Part of the mechanism for EPO-induced cardio-protection m...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2005-04-01 00:00:00
abstract：:Four commercial assays for the screening of cannabinoids in urine were compared. Urine specimens from 93 selected subjects were run by fluorescence polarization immunoassay on the Abbott TDx; by enzyme multiplied immunoassay with two Syva EMIT assays; and by thin-layer chromatography with the TOXI-LAB system (Marion L...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
abstract：:A brief historical review is presented of the development of bovine serum albumin as a standard for the determination of serum total protein. Bovine serum albumin is now available as a standard reference material from the National Bureau of Standards. Also reviewed is the present status of the immunoglobulin standards...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1978-05-01 00:00:00
abstract：:An in-house method was used, including dissociation of Somatomedin C/insulin-like Growth Factor I (Sm-C/IGF-I) binding proteins with acid and extraction of Sm-C/IGF-I by C-2 cartridge before radioimmunoassay, to measure plasma Sm-C/IGF-I levels in three groups of patients--total of 19--with acromegaly who underwent tr...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1992-03-01 00:00:00
abstract：:The Access(®) soluble transferrin receptor (sTfR) is considered the world's first automated chemiluminescence immunoassay. In this study, the diagnostic utility of this and other tests for serum iron were evaluated by studying their interrelationships with inflammation. A total of 367 patients with anemia (iron defici...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2015-07-01 00:00:00
abstract：:To identify markers sensitive to inhibitors of the farnesylation pathway, we used 3 breast cancer cell lines (SKBR-3, MDA-175, and MDA-231) to evaluate the in vitro effects of pamidronate, an inhibitor of farnesyl diphosphate synthase. In response to pamidronate, there was significant inhibition of cell proliferation ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2004-07-01 00:00:00
abstract：:Reactive amyloid deposits consist of amyloid A (AA) proteins, the degradation products of serum amyloid A (SAA). Since the most common species of AA is the amino terminal portion produced by cleavage between residues 76 and 77 of SAA (AA76), the presence of AA76 in tissues could be a consequence of AA amyloid depositi...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2016-04-01 00:00:00
abstract：:Iron accumulation, especially that of free oxidized ferrous iron, has been shown to induce tissue oxidative damage and contribute to brain aging and the development of neurodegenerative disease. Here we examine whether sex and advanced age affect the expression of iron-related molecules that participate in regulating ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2012-04-01 00:00:00
abstract：:We report a case of a 69-yr-old previously healthy man with acute spontaneous Achilles tendon rupture and severe tendonitis, which occurred after 2 weeks of steroid therapy for newly diagnosed giant cell arteritis. The Achilles tendon rupture was treated conservatively and the tendonitis resolved incrementally with st...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2003-07-01 00:00:00
abstract：:In a prospective study, M4RT medium was compared to the traditional M4 medium to transport cervical swab specimens for Neisseria gonorrhoeae/Chlamydia trachomatis (NG/CT) PCR testing using the Roche COBAS Amplicor. Two cervical swab samples were collected from 270 consecutive patients screened for NG/CT in a satellite...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:The aim of the study was to determine if amniotic fluid cells of rats can be used to provide evidence of genotoxicity. In order to do that micronuclei formation was induced in rats during pregnancy after treatment with cyclophosphamide (CP), at different CP doses. On gestational day 19, we collected the amniotic fluid...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2018-03-01 00:00:00
abstract：:A quality control program of analyses of toxic metals in urine has been carried out in the Nordic countries since 1978. In connection with these programs, the advantages and disadvantages of lyophilized compared to natural urine specimens as control materials have been investigated in three successive similar studies....
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1987-05-01 00:00:00
abstract：:Lidocaine, quinidine, and theophylline binding to normal pooled mature human milk was determined using equilibrium dialysis at 4 degrees C. Binding to milk was compared with binding to pooled human serum. The observed binding was related to the relative lipid solubility of each drug in milk. The potential for the excr...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：:One hundred thirty-three Streptococcus milleri group (S. anginosus) isolates were recovered from 487 surgical patients. The streptococci were recovered from 33 percent of intra-abdominal infection cultures (84/257). 22 percent of samples from penetrating visceral trauma (19/86), 52 percent of perirectal abscess specim...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:Certain DNA-binding proteins that regulate gene expression contain single or multiple copies of short polypeptide sequences, approximately 30 residues long, consisting of combinations of four Cys or His residues at defined spacing, so that Zn++ is complexed in tetrahedral coordination with the respective thiol-sulfur ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章,评审
doi：
更新日期：1988-07-01 00:00:00
abstract：:To investigate the relationships of trace element concentrations vs hematopoiesis and iron parameters, we measured lead, copper, zinc, cadmium, and ferritin levels in 251 healthy adolescents. Concentrations of trace metals were determined by atomic absorption spectrophotometry. There were no significant gender-related...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2005-10-01 00:00:00
abstract：:We report a papillary glioneuronal tumor occurring in the right frontal lobe of a 26-yr-old woman and we review the pertinent literature. Papillary glioneuronal tumor (PGNT) is a rare cerebral neoplasm, identified in approximately 37 cases to date. In 2007, the World Health Organization (WHO) classified the PGNT as a ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章,评审
doi：
更新日期：2008-07-01 00:00:00
abstract：:Spurious macrocytosis in electronic cell counters has been associated with hyperglycemia. The increased mean corpuscular volume (MCV) is thought to be secondary to osmotic disequilibrium between the erythrocyte and the diluent used in the automatic cell counters. Employing three different hematology cell counters, the...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1985-07-01 00:00:00
abstract：:This paper is a summary of studies over the past few years that pertain to animal neurotoxins. These toxins are found throughout the animal kingdom. Homologies exist in the structures of these poisons within classes and point to conservation of active sites throughout evolution. In the case of the peptides, invariant ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1975-09-01 00:00:00
abstract：GOALS:This study aims to examine the effects of raising the reporting age for benign endometrial cells (BEC) on Papanicolaou (Pap) tests according to the 2014 3rd edition of the Bethesda System for Cervical Cytology, as well as the sequelae of the 2012 American Society of Colposcopy and Cervical Pathology (ASCCP) guide...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2017-05-01 00:00:00
abstract：:Variations in the level of prealbumin in serum and spinal fluid were noted on routine electrophoresis. Low serum levels were associated with infection, liver disorders, and malignant disease, whereas high levels were seen in inflammatory disorders. A high spinal fluid prealbumin was noted in a young child with a disse...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1987-09-01 00:00:00
