Abstract:
:In a prospective study, M4RT medium was compared to the traditional M4 medium to transport cervical swab specimens for Neisseria gonorrhoeae/Chlamydia trachomatis (NG/CT) PCR testing using the Roche COBAS Amplicor. Two cervical swab samples were collected from 270 consecutive patients screened for NG/CT in a satellite facility. The swabs were placed individually in M4RT and M4 medium and were immediately refrigerated, transported to the laboratory on wet ice, and stored at 2 to 8 degrees C until the PCR testing was performed within 7 da of collection. Seven of the cervical swab samples transported in M4 or M4RT were PCR positive for CT. Two additional samples transported in M4RT and a third swab transported in M4 were CT PCR positive. These samples were PCR negative in the alternative medium. Similarly, 12 of the cervical swabs transported in M4 or M4RT were NG PCR positive. Three additional swabs transported in M4 media were NG PCR positive. Initially, 2 of these samples when transported in M4RT were NG PCR equivocal and were considered NG PCR positive on repeat testing. Similarly, 2 additional swab samples transported in M4 RT media were NG PCR positive. These samples, when transported in M4 media, were NG PCR equivocal or negative. However, on repeat testing the equivocal sample was considered NG PCR positive. We conclude M4 and M4RT transport media are equally reliable for transporting cervical swab samples for NG/CT PCR testing. M4RT medium is more convenient to use, as it did not require refrigeration until it was inoculated with the clinical sample.
journal_name
Ann Clin Lab Scijournal_title
Annals of clinical and laboratory scienceauthors
Aslanzadeh J,Jones Msubject
Has Abstractpub_date
2002-01-01 00:00:00pages
61-4issue
1eissn
0091-7370issn
1550-8080journal_volume
32pub_type
杂志文章abstract::Human amniotic-derived cells (hAMCs) have recently raised interest for their differentiation capability and immunomodulatory properties. To assess the feasibility of hAMCs therapeutic treatment during fetal development, we explored the localization of cells derived from the human amniotic membrane in rat organs after ...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2015-04-01 00:00:00
abstract::The prognostic potentials of physiological ([Acute Physiologic and Chronic Health Evaluation] APACHE II score) and biochemical (Interleukin 6 [IL-6], Interleukin 6 soluble receptor [IL-6sR], and Interleukin 2 receptor [IL-2R], nitrates) measures were evaluated in sepsis. The APACHE II scores were calculated, and conce...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1996-09-01 00:00:00
abstract::Patients with diabetes mellitus have an increased risk of thrombosis and accelerated atherogenesis. Increased platelet adhesion and aggregation are noted in vitro. This paper reviews known platelet abnormalities found in patients with diabetes mellitus (DM) and examines the pathophysiology associated with these abnorm...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章,评审
doi:
更新日期:1993-01-01 00:00:00
abstract::The anucleate mature erythrocyte also lacks ribosomes and mitochondria and thus cannot synthesize enzymes or derive energy from the Krebs citric acid cycle. Nevertheless, the red blood cell is metabolically active and contains numerous residual enzymes and their products which are essential for its survival and normal...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章,评审
doi:
更新日期:1980-09-01 00:00:00
abstract::To evaluate the screening power of the fecal cytokeratin-19 test (CK-19) and the fecal occult blood test (FOBT), we performed rapid fecal CK-19 and FOBT tests on 515 stool samples from patients with various GI diseases and 814 stool samples from control patients. The rapid fecal CK-19 test (developed by DiNonA Researc...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2006-07-01 00:00:00
abstract::Until recently it has been generally accepted that the choroid plexuses are the major source of the cerebrospinal fluid (CSF). However, data now indicate that significant contributions to the CSF are made at extrachoroidal sites. CSF production studies and 24Na transport studies in normal and plexectomized rhesus monk...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1976-01-01 00:00:00
abstract::Allergic rhinosinusitis involves several types of inflammatory cells. The dominant inflammatory cells include mast cells, eosinophils, lymphocytes, and monocytes/macrophages. Since eosinophils are one type of inflammatory cell that is often related to allergy, we investigated in this study whether the eosinophils pres...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2007-04-01 00:00:00
abstract::Ovarian cancer is the leading cause of death due to malignant tumors in female reproductive organs. However, the lack of understanding regarding its pathogenesis brings difficulties to study it. In this study, we analyze the differently expressed genes in both GSE54388 (ovarian cancer vs. normal ovarian tissues) and G...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2018-09-01 00:00:00
abstract::Spermatogenesis is severely altered in the nonobese diabetic (NOD) mouse in the presence of diabetes. When insulin is administered early in the development of diabetic changes, blunting of the testicular damage results, suggesting a direct causal effect of hyperglycemia on the testicular alterations. In view of the ke...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
abstract::One hundred thirty-three Streptococcus milleri group (S. anginosus) isolates were recovered from 487 surgical patients. The streptococci were recovered from 33 percent of intra-abdominal infection cultures (84/257). 22 percent of samples from penetrating visceral trauma (19/86), 52 percent of perirectal abscess specim...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::A fifteen-year-old girl was treated with isoniazid (INH) for latent tuberculosis infection (LTBI), and subsequently developed epigastric pain, vomiting, and jaundice after three months of treatment. Acute fulminant hepatic failure was diagnosed. INH was stopped, and she received N-acetyl cysteine and Vitamin K. Liver ...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract::Very recently there have been sporadic reports of polymyositis in patients who are positive for human immunodeficiency virus (HIV). The cause of this condition has not been documented. Recent evidence has been presented which indicates that the Coxsackie B virus may be a causative factor. Presentation is made of a pat...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1994-05-01 00:00:00
abstract::Metabolic acidosis secondary to lactic acidosis may occur in acute, severe asthma and its presence suggests that respiratory muscle fatigue and tissue hypoxia play a major part in the pathogenesis. Non-anion gap metabolic acidosis (NAG acidosis) has also been reported in acute asthma but its impact on the clinical out...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2008-07-01 00:00:00
abstract::A variety of vaginal and cervical abnormalities have been encountered in the offspring of women who have taken stilbestrol or chemically related nonsteroidal estrogens during pregnancy. Cervical erosion has been noted most often, but vaginal adenosis has been proven by biopsy in over 30 percent, and transverse vaginal...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1974-07-01 00:00:00
abstract::In vitro determination of allergen-specific IgE (sIgE) represents an important aid in the diagnosis and treatment of allergy. Improvements in laboratory methodology--the development of second, and now third generation assays for sIgE--have brought about major advances in speed, convenience, performance, and in the sta...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::Primary adenocarcinoma of the fallopian tube is a rare malignant neoplasm of the female reproductive system. Unusual patterns of metastasis may be observed. Report is made of a patient with recurrent adenocarcinoma of the fallopian tube, which presented as leptomeningeal carcinomatosis, as a first site of distant meta...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1996-03-01 00:00:00
abstract::Qualitative urinary screening procedures were converted to quantitative methods for urinary cystine and homocystine based on the reactions between these amino acids and cyanide-nitroprusside reagents. Cystine and homocystine are quantified by the measurement of absorbances at 521 and 524 nm, respectively. Cyanide-nitr...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract::Cupric chloride (CuCl2) and zinc chloride (ZnCl2) were tested by the FETAX (Frog Embryo Teratogenesis Assay: Xenopus) procedure in the South African frog, Xenopus laevis. The median teratogenic concentrations (EC50) of Cu2+ and Zn2+ were 2.5 and 40 mumol per L. The median embryolethal concentrations (LC50) of Cu2+ and...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1993-03-01 00:00:00
abstract::An unusual case is reported of pleomorphic large cell sarcoma of the spleen with rhabdomyosarcomatous differentiation in a 34-year old male. According to our knowledge, such a neoplasm has never been reported in the literature. ...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1999-10-01 00:00:00
abstract:CONTEXT:Hepatitis C virus (HCV) infects up to 1.8% of the US general population, although the rate is significantly higher in military veterans at 5.4-20%. Early detection and accurate diagnosis are critical as chronic HCV infection can lead to liver cirrhosis and hepatocellular carcinoma. Genotype analysis has both th...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2012-10-01 00:00:00
abstract::Hydroxyethyl starch (HES) has been shown to be beneficial in several inflammatory situations, but the mechanisms are unclear. The present study tested the hypothesis that HES has effects on nuclear factor kappa B (NF-kappaB) activation and the expression of inflammatory mediators induced by lipopolysaccharide. Sepsis ...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2003-10-01 00:00:00
abstract::Hemoglobin H (Hb H) disease is usually characterized by the existence of Hb H, which influences the degree of functional anemia. We here report a patient with a rare Hb H disease genotype (-SEA/-α27.6), who was observed to paradoxically have no detectable Hb H fraction on electrophoresis. To date, the reason why the q...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2018-11-01 00:00:00
abstract::Although it is widely accepted that patients with immune thrombocytopenia produce platelet antibodies, the demonstration of such antibodies has been difficult and time consuming. A simple and quick enzyme linked immunoassay for platelet antibodies is presented. The platelet associated IgG is coupled with alkaline phos...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract:BACKGROUND:Long-term use of hormones and immunosuppressive agents is necessary for treating polymyositis (PM)/dermatomyositis (DM) but may cause liver damage. At what point do the costs of treatment outweigh the benefits, and how will the breakeven point be determined? METHODS:Serum muscle enzyme changes in 93 PM/DM p...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2016-05-01 00:00:00
abstract::Renal arterioles from five patients with benign essential hypertension (BH) and four patients with malignant essential hypertension (MH) were studied using electron microscopy (EM). This study suggests a concept of different pathogenetic mechanism for renal vascular (arteriolar) lesions in BH and MH (i.e., benign and ...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1977-03-01 00:00:00
abstract::The fragile histidine triad (FHIT) gene encompasses the most common human fragile site, FRA3B at 3p14.2, a region that is involved in homozygous deletions in a variety of human tumors. FHIT is considered to be a tumor suppressor gene that is frequently inactivated in various types of cancer. To study the role of the F...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2010-07-01 00:00:00
abstract::Small marker chromosomes (SMC) associated with severe Turner syndrome (TS) variants often represent reduced X chromosomes lacking the X inactivation center (XIC), perturbed dosage compensation, and unbalanced gene expression. A TS patient with mental retardation (MR), unusually short stature, facial and limb malformat...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::Various fungal products, such as gliotoxin (GT), have immunomodulating activity, a fact exploited previously by our group for prevention of autoimmune diabetes mellitus in BB/Wor rats. To understand better the immunologic effects in GT-treated rats, splenocytes from 65-day-old prediabetic diabetes-prone rats were phen...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2000-10-01 00:00:00
abstract::Diabetes-induced hyperglycemia has a direct damaging effect on ovarian function. Despite its deadly impact on ovaries, the mechanism of this condition has not been fully elucidated. Glucose transporters are involved in glucose uptake and utilization. Many transporters have been detected in the ovaries, but their roles...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2019-11-01 00:00:00
abstract::HbA1 is the result of the non-enzymatic glycosylation of HbA. HbA1 reflects the integrated blood glucose level that prevailed over a period of weeks; it thus has clinical application in the assessment of the long-term blood glucose control. This ability to determine easily the long-term blood glucose should enable the...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章,评审
doi:
更新日期:1980-03-01 00:00:00