Abstract：
:The fragile histidine triad (FHIT) gene encompasses the most common human fragile site, FRA3B at 3p14.2, a region that is involved in homozygous deletions in a variety of human tumors. FHIT is considered to be a tumor suppressor gene that is frequently inactivated in various types of cancer. To study the role of the FHIT gene in thyroid tumorigenesis, we looked for homozygous deletions or mutations of exons 5 and 8 of the FHIT gene in 65 cases of differentiated thyroid carcinoma (DTC) and their matched non-cancerous epithelium (NCE), using exon-specific PCR amplification and PCR single strand conformation polymorphism (PCR-SSCP) techniques. In DTC, the incidence of homozygous deletion of exon 5 was 30.8% (20/65), and it was associated with tumor metastasis to lymph nodes (p <0.05). The incidence of homozygous deletion of exon 8 was 29.2% (19/65), and it was associated with the tumor pathological grade, TNM stage, and lymph node metastasis (p <0.05). There was strong correlation between homozygous deletions of exon 5 and exon 8 (p <0.01). No point mutations were observed in either exon 5 or exon 8. These findings suggest that: (a) exons 5 and 8 of FHIT are key target regions of deletion, (b) homozygous deletions of exon 5 and exon 8 may be good biomarkers for the biological behavior of DTC, and (c) point mutation of these exons may not be involved in the inactivation of the FHIT gene in DTC.
journal_name
Ann Clin Lab Scijournal_title
Annals of clinical and laboratory scienceauthors
Yin DT,Wang L,Sun J,Yin F,Yan Q,Shen RL,Gao JX,He Gsubject
Has Abstractpub_date
2010-07-01 00:00:00pages
267-72issue
3eissn
0091-7370issn
1550-8080pii
40/3/267journal_volume
40pub_type
杂志文章abstract：:A simple determination of 5'-Nucleotidase in blood serum without deproteinization is described. The enzyme activity is distinguished from that of a nonspecific alkaline phosphatase by nickel inhibition and the inorganic phosphate released from adenosine monophosphate used as substrate is determined by a method previou...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1977-11-01 00:00:00
abstract：OBJECTIVE:MicroRNAs (miRNAs) are associated with hepatocellular carcinoma (HCC) progression and metastasis. However, it is unclear whether they could act as biomarkers for HCC diagnosis and prognosis. METHODS:In this study, the miR-122 and miR-199a expression levels were determined by quantitative real-time PCR (qRT-P...
journal_title：Annals of clinical and laboratory science
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journal_title：Annals of clinical and laboratory science
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journal_title：Annals of clinical and laboratory science
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doi：
更新日期：1978-03-01 00:00:00
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journal_title：Annals of clinical and laboratory science
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journal_title：Annals of clinical and laboratory science
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journal_title：Annals of clinical and laboratory science
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
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更新日期：1986-01-01 00:00:00
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journal_title：Annals of clinical and laboratory science
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更新日期：1980-03-01 00:00:00
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journal_title：Annals of clinical and laboratory science
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
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更新日期：1978-09-01 00:00:00
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journal_title：Annals of clinical and laboratory science
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journal_title：Annals of clinical and laboratory science
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更新日期：2014-10-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1975-09-01 00:00:00
abstract：:Male Swiss Webster mice were fed a diet containing 2.5 percent griseofulvin (GF). Serially progressive light and electron microscopic hepatic altertions were present from one day on and grossly visible hepatomegaly from two weeks until termination at 22 weeks. GF induced liver changes included hepatocyte nuclear and c...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
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journal_title：Annals of clinical and laboratory science
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更新日期：2013-01-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章,评审
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journal_title：Annals of clinical and laboratory science
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journal_title：Annals of clinical and laboratory science
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更新日期：1997-11-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章,评审
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更新日期：1981-05-01 00:00:00
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journal_title：Annals of clinical and laboratory science
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更新日期：2015-10-01 00:00:00
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journal_title：Annals of clinical and laboratory science
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journal_title：Annals of clinical and laboratory science
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更新日期：1979-11-01 00:00:00
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journal_title：Annals of clinical and laboratory science
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更新日期：1978-05-01 00:00:00
abstract：:In vitro activity of erythrocyte glucose-6-phosphate dehydrogenase (G-6-PD) does not always correlate with in vivo hemolytic manifestations. Many of these non-correlations are reviewed and can now be explained on the basis of altered substrate affinity and/or altered inhibition by intracellular metabolites. These alte...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1977-07-01 00:00:00
