Abstract：
:The fragile histidine triad (FHIT) gene encompasses the most common human fragile site, FRA3B at 3p14.2, a region that is involved in homozygous deletions in a variety of human tumors. FHIT is considered to be a tumor suppressor gene that is frequently inactivated in various types of cancer. To study the role of the FHIT gene in thyroid tumorigenesis, we looked for homozygous deletions or mutations of exons 5 and 8 of the FHIT gene in 65 cases of differentiated thyroid carcinoma (DTC) and their matched non-cancerous epithelium (NCE), using exon-specific PCR amplification and PCR single strand conformation polymorphism (PCR-SSCP) techniques. In DTC, the incidence of homozygous deletion of exon 5 was 30.8% (20/65), and it was associated with tumor metastasis to lymph nodes (p <0.05). The incidence of homozygous deletion of exon 8 was 29.2% (19/65), and it was associated with the tumor pathological grade, TNM stage, and lymph node metastasis (p <0.05). There was strong correlation between homozygous deletions of exon 5 and exon 8 (p <0.01). No point mutations were observed in either exon 5 or exon 8. These findings suggest that: (a) exons 5 and 8 of FHIT are key target regions of deletion, (b) homozygous deletions of exon 5 and exon 8 may be good biomarkers for the biological behavior of DTC, and (c) point mutation of these exons may not be involved in the inactivation of the FHIT gene in DTC.
journal_name
Ann Clin Lab Scijournal_title
Annals of clinical and laboratory scienceauthors
Yin DT,Wang L,Sun J,Yin F,Yan Q,Shen RL,Gao JX,He Gsubject
Has Abstractpub_date
2010-07-01 00:00:00pages
267-72issue
3eissn
0091-7370issn
1550-8080pii
40/3/267journal_volume
40pub_type
杂志文章abstract：:A retrospective study of acquired renal lesions in 42 renal transplants in children revealed a variety of histologic lesions. Although acute and chronic rejection changes were the most frequent, a number of metabolic, infectious, vascular, and other lesions were encountered. Renal biopsy allows rapid diagnosis of thes...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1981-07-01 00:00:00
abstract：:The histogenesis of blasts in acute myelofibrosis is generally regarded to be of megakaryocytic origin. Three case reports are presented and 19 other reported cases were reviewed from the literature where the cells of origin appear to be myeloblasts, myelomonoblasts, lymphoblasts, or undifferentiated blasts. It is the...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章,评审
doi：
更新日期：1990-11-01 00:00:00
abstract：:Pancreatic cancer is one of the most lethal diseases among solid malignancies. Most patients are diagnosed in the late stage or after metastasis. Notably, pancreatic cancer is resistant to chemotherapy and lacks efficient target therapy methods. Given that, investigating the mechanism of metastasis, finding biomarkers...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章,评审
doi：
更新日期：2019-05-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1981-09-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2015-10-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
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更新日期：2011-10-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2015-07-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1983-11-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2015-10-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章,评审
doi：
更新日期：2011-07-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2014-07-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章,评审
doi：
更新日期：1993-05-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2012-04-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1995-07-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2008-07-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1990-03-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2006-04-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2017-08-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1994-05-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1987-05-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1987-03-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2017-05-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2001-10-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1993-09-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2002-07-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2006-04-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1975-07-01 00:00:00
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journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1980-11-01 00:00:00
