Abstract:
:It has been shown that the single nucleotide polymorphism (SNP) of the rs2735940 site in the human telomerase reverse transcriptase (hTERT) gene is associated with increased cancer risk. The traditional method to detect SNP genotypes is polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). However, there is a limitation to utilizing PCR-RFLP due to a lack of proper restriction enzyme sites at many polymorphic loci. This study used an improved PCR-RFLP method with a mismatched base for detection of the SNP rs2735940. A new restriction enzyme cutting site was created by created restriction site PCR (CRS-PCR), and in addition, the restriction enzyme MspI for CRS-PCR was cheaper than other enzymes. We used this novel assay to determine the allele frequencies in 552 healthy Chinese Han individuals, and found the allele frequencies to be 63% for allele C and 37% for allele T In summary, the modified PCR-RFLP can be used to detect the SNP of rs2735940 with low cost and high efficiency.
journal_name
Ann Clin Lab Scijournal_title
Annals of clinical and laboratory scienceauthors
Wang S,Ding M,Duan X,Wang T,Feng X,Wang P,Yao W,Wu Y,Yan Z,Feng F,Yu S,Wang Wsubject
Has Abstractpub_date
2017-09-01 00:00:00pages
546-550issue
5eissn
0091-7370issn
1550-8080pii
47/5/546journal_volume
47pub_type
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