Changes in Expression of Follicular Glucose Transporters May Be Involved in Ovarian Function Impairment during Diabetic Hyperglycemia.

Abstract:

:Diabetes-induced hyperglycemia has a direct damaging effect on ovarian function. Despite its deadly impact on ovaries, the mechanism of this condition has not been fully elucidated. Glucose transporters are involved in glucose uptake and utilization. Many transporters have been detected in the ovaries, but their roles in diabetes-induced ovarian impairment are still unclear. In this study, the goal is to analyze glucose transporter expression in the ovarian follicles of type 1 diabetes mellitus patients and determine their roles within ovarian function impairment. The ovarian function of a mouse model of type 1 diabetes mellitus was evaluated by observing its estrus cycle, follicular development, and ovulation. Subtypes of the glucose transporter (GLUT2, GLUT3, GLUT4, SGLT1, and SGLT2), adenosine monophosphate-activated protein kinase (AMPK), and phosphorylated AMPK (Thr172) were found to be simultaneously present in follicle cells. Compared with nondiabetic control mice, the diabetic mice showed a dysregulated estrus cycle and a significantly higher number of abnormal ova. Furthermore, the expression of multiple glucose transporters was lower than that of phosphorylated AMPK. Phosphorylated AMPK possessed more follicular granulosa cells and oocytes of diabetic mice than in those of the control mice. These results suggest that diabetes-induced hyperglycemia reduces the capability of ovarian follicle cells by downregulating glucose transporter expression, causing decreased glucose uptake and energy deprivation. This impact can potentially impair egg maturation and ovulation.

journal_name

Ann Clin Lab Sci

authors

Xu B,Hu Y,Luo YT,Xu H,Qi SH,Zhang YY,Zhao D,Yuan DZ

subject

Has Abstract

pub_date

2019-11-01 00:00:00

pages

785-793

issue

6

eissn

0091-7370

issn

1550-8080

pii

49/6/785

journal_volume

49

pub_type

杂志文章
  • Strategic utilization of cardiac markers for the diagnosis of acute myocardial infarction.

    abstract::Current changes of the healthcare environment have promoted the creation of chest pain centers in the emergency departments for rapid triage of patients admitted for cardiac evaluation. Because of the inefficiency of electrocardiogram for the diagnosis of acute myocardial infarction, blood cardiac markers play an impo...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章

    doi:

    authors: Wong SS

    更新日期:1996-07-01 00:00:00

  • Plasma Cell Granuloma: An Entity within the Spectrum of IgG4-Related Disease.

    abstract::Plasma cell granuloma (PCG) is a relatively rare, mass-forming lesion comprised of polyclonal plasma cells set in a background of storiform fibrosis and spindle cell proliferation. While uncommon, this lesion may occur within any site and should be included in the list of differential diagnoses for plasma cell neoplas...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章

    doi:

    authors: Forcucci J,Butler-Williams S,Miller N,Lazarchick J

    更新日期:2015-04-01 00:00:00

  • Pathogenesis of post primary tuberculosis: immunity and hypersensitivity in the development of cavities.

    abstract::M. Tuberculosis (MTB) is an obligate human parasite even though humans are more resistant than any of the animals used for study. It is a human parasite because only humans develop post primary tuberculosis (TB) in their lungs that mediates transmission of infection to new hosts. The extreme paucity of human lung tiss...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章

    doi:

    authors: Hunter RL,Actor JK,Hwang SA,Karev V,Jagannath C

    更新日期:2014-10-01 00:00:00

  • Assessment of mitochondrial function in cells grown in tissue culture.

    abstract::To assess mitochondrial function (pyruvate dehydrogenase [PDH] activity), cells were grown in the appropriate media to confluence, rinsed and incubated in glucose free media containing 25 microM L-lactate and [1-14C]-D,L-lactate. Lactate oxidation was measured as the amount of lactate oxidized in nmol of 14CO2 generat...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章

    doi:

    authors: Ofenstein JP,Dandurand DM,Kiechle FL

    更新日期:1992-11-01 00:00:00

  • Parotid gland abscess caused by Haemophilus paraphrophilus: a case report.

    abstract::Human diseases caused by Haemophilus paraphrophilus (H.paraphrophilus) are unusual. The following case report describes a 67-year-old man who presented with pain and swelling of the right side of the face. Fine needle aspiration suggested a parotid gland abscess. Microbiological studies identified H.paraphrophilus. Th...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章

    doi:

    authors: Rets AV,Eid I,Lutwick L,Pincus MR

    更新日期:2012-10-01 00:00:00

  • Acute severe rhabdomyolysis in an human immunodeficiency virus-seropositive patient associated with rising anti-coxsackie B viral titers.

    abstract::Very recently there have been sporadic reports of polymyositis in patients who are positive for human immunodeficiency virus (HIV). The cause of this condition has not been documented. Recent evidence has been presented which indicates that the Coxsackie B virus may be a causative factor. Presentation is made of a pat...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章

    doi:

    authors: Beressi A,Sunheimer RL,Huish S,Finck C,Pincus MR

    更新日期:1994-05-01 00:00:00

  • Amyloid deposition in Alzheimer's disease.

    abstract::Alzheimer's disease (AD), a progressive neurodegenerative disorder, accounts for approximately 60 percent of all victims of dementia and affects greater than 10 percent of the population over 65 years old. Although the cause is unknown, there is evidence that beta-amyloid plays an important role in its pathogenesis. T...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章,评审

    doi:

    authors: Meena-Leist CE,Parker JC Jr

    更新日期:1993-05-01 00:00:00

  • Comparison of VIDAS Stallertest and Pharmacia CAP assays for detection of specific IgE antibodies in allergic children.

    abstract::In vitro determination of specific IgE antibodies in serum is the most frequently used method, besides the skin test, for diagnosing allergies. Standardized and reproducible assays of specific IgE antibodies contribute to the quality of diagnosis and treatment of allergic disease. This study compared the results and p...

    journal_title:Annals of clinical and laboratory science

    pub_type: 临床试验,杂志文章

    doi:

    authors: Sohn MH,Lee SY,Lee KE,Kim KE

    更新日期:2005-07-01 00:00:00

  • Optimization PCR for Detection CTG/CCTG-Repeat Expansions in the Diagnosis of Myotonic Dystrophies.

    abstract:CONTEXT:Myotonic dystrophies (DMs) are a group of autosomal dominant neuromuscular disorders which are caused by large CTG/CCTG-repeat expansions in untranslated regions of DMPK/ZNF9 gene. The "phenotypic overlap" in DMs creates complication in distinguishing patients with DM1 from patients with DM2 and underscores the...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章

    doi:

    authors: Meng YX,Shen HR,Zhao Z,Bing Q,Li N,Hu J

    更新日期:2015-10-01 00:00:00

  • Restoration of DLC1 gene inhibits proliferation and migration of human colon cancer HT29 cells.

    abstract::DLC1 (deleted in liver cancer-1) is a new candidate tumor suppressor gene, which is inactive in various types of human cancers including colon cancer. To study the function of DLC1, we constructed a pcDNA3.1 vector containing the DLC1 gene and transfected it into HT29 colon cancer cells that were deficient in DLC1 exp...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章

    doi:

    authors: Wu PP,Jin YL,Shang YF,Jin Z,Wu P,Huang PL

    更新日期:2009-07-01 00:00:00

  • Effect of increasing glucose concentrations on Sertoli cell viability in the nonobese diabetic mouse.

    abstract::Spermatogenesis is severely altered in the nonobese diabetic (NOD) mouse in the presence of diabetes. When insulin is administered early in the development of diabetic changes, blunting of the testicular damage results, suggesting a direct causal effect of hyperglycemia on the testicular alterations. In view of the ke...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章

    doi:

    authors: Gondos B,Rivkind Y,Jovanovic L

    更新日期:1998-07-01 00:00:00

  • Pamidronate resistance and associated low ras levels in breast cancer cells: a role for combinatorial therapy.

    abstract::To identify markers sensitive to inhibitors of the farnesylation pathway, we used 3 breast cancer cell lines (SKBR-3, MDA-175, and MDA-231) to evaluate the in vitro effects of pamidronate, an inhibitor of farnesyl diphosphate synthase. In response to pamidronate, there was significant inhibition of cell proliferation ...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章

    doi:

    authors: Zhang PL,Lun M,Siegelmann-Danieli N,Blasick TM,Brown RE

    更新日期:2004-07-01 00:00:00

  • Major Discrepancies of HCV Viral Loads Determined by Real Time Polymerase Chain Reaction Caused by Use of Collection Tubes that Differ Only with the Presence or Absence of Serum Separator.

    abstract:BACKGROUND:For viral load (VL) analysis of hepatitis C virus (HCV) by Real Time PCR, approved collection tubes were lavender top (LTT) and plasma processing (PPT) tubes, that differ in that PPTs include plasma separator. Using a Real Time PCR method, we investigated how the results correlated for the two tube types. M...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章

    doi:

    authors: Wang X,Momeni A,Eid I,Li L,Brackett F,Davis-Smith B,Wieczorek R,Pincus MR

    更新日期:2018-05-01 00:00:00

  • Intravascular large B-cell lymphoma presenting as cholecystitis and pancytopenia: case report with literature review.

    abstract::Intravascular large B-cell lymphoma (IVLBCL) is a rare variant of extranodal diffuse large Bcell lymphoma with only a few more than 300 cases reported. It is characterized as lymphoma cells confined to the lumina of small vessels, so patients usually do not present with masses or lymphadenopathy. Clinical presentation...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章,评审

    doi:

    authors: Duan X,Lapus A,Brown RE,Chen L

    更新日期:2011-07-01 00:00:00

  • Lidocaine, quinidine, and theophylline binding to human milk.

    abstract::Lidocaine, quinidine, and theophylline binding to normal pooled mature human milk was determined using equilibrium dialysis at 4 degrees C. Binding to milk was compared with binding to pooled human serum. The observed binding was related to the relative lipid solubility of each drug in milk. The potential for the excr...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章

    doi:

    authors: Bailey DN

    更新日期:1998-09-01 00:00:00

  • Pathogenic analysis of Aeromonas hydrophila septicemia.

    abstract::Aeromonas hydrophila has emerged as a potential pathogen in the immunocompromised host. Various aeromonal infections, including septicemia, have also been reported in apparently healthy individuals. For years, researchers have disagreed over the epidemiologic roles of aeromonads in gastroenteritis. Isolation rates of ...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章

    doi:

    authors: Chang CY,Thompson H,Rodman N,Bylander J,Thomas J

    更新日期:1997-07-01 00:00:00

  • Analytical Performance Evaluation of Aptima Herpes Simplex Virus 1 and 2 Assay Using Hologic Panther Instrument.

    abstract:OBJECTIVE:Herpes simplex viruses 1 and 2 (HSV-1 and HSV-2) are responsible for a variety of human diseases. Therefore, rapid detection of these viruses in clinical laboratory is important. Recently Aptima Herpes Simplex 1 and 2 assay has been approved by the FDA. We evaluated analytical performance of this assay by com...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章

    doi:

    authors: Dixon RB,Bonaparte J,Greenwood CJ,Davis M,Dasgupta A

    更新日期:2020-03-01 00:00:00

  • Carbon monoxide in firemen before and after exposure to smoke.

    abstract::A statistically significant difference was found between the mean baseline carboxyhemoglobin of non-smoking firemen (COHb 2.53, sd = 1.05, n = 32) and smoking firemen (COHb 5.54, sd = 2.61, n = 26). A consistent increase in mean COHb levels after exposure to smoke was seen in both non-smoking (4.41 sd = 2.95, n = 7) a...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章

    doi:

    authors: Levy AL,Lum G,Abeles FJ

    更新日期:1976-09-01 00:00:00

  • Free radicals: their history and current status in aging and disease.

    abstract::Oxygen toxicity was first described in laboratory animals in 1878 and was further established in 1899. The first experiment regarding a free radical reaction was reported in 1894. However, it was not until the late 1940s to early 1950s that retrolental fibroplasia in premature newborns was recognized as being due to o...

    journal_title:Annals of clinical and laboratory science

    pub_type: 历史文章,杂志文章,评审

    doi:

    authors: Knight JA

    更新日期:1998-11-01 00:00:00

  • Clinical consequences of enzyme deficiencies in the erythrocyte.

    abstract::The anucleate mature erythrocyte also lacks ribosomes and mitochondria and thus cannot synthesize enzymes or derive energy from the Krebs citric acid cycle. Nevertheless, the red blood cell is metabolically active and contains numerous residual enzymes and their products which are essential for its survival and normal...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章,评审

    doi:

    authors: Netzloff ML

    更新日期:1980-09-01 00:00:00

  • In vitro production of monoclonal human rheumatoid factors.

    abstract::Lymphocytes from rheumatoid arthritis patients have been fused with drug-marked human myeloma cell lines and hybrids selected which secrete rheumatoid factors. Limiting dilution and soft agarose plates have been used to clone these hybrids, but so far stable clones have not been obtained. Epstein-Barr virus has been u...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章

    doi:

    authors: Normansell DE,Lane JR

    更新日期:1984-01-01 00:00:00

  • Comparison of the Abbott Architect i2000 assay, the Roche Modular Analytics E170 assay, and an immunoradiometric assay for serum hepatitis B virus markers.

    abstract::Serum hepatitis B virus (HBV) markers are the most important data for epidemiological screening and clinical diagnosis of HBV infection, especially in endemic areas. We compared the results of the Roche Modular Analytics E170 assay, the Abbott Architect i2000 assay, and an immunoradiometric assay (IRMA) for HBV surfac...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章

    doi:

    authors: Kim H,Oh EJ,Kang MS,Kim SH,Park YJ

    更新日期:2007-07-01 00:00:00

  • Serum C1q Concentration Positively Correlates with Erythrocyte Sedimentation Rate in Polymyositis/Dermatomyositis.

    abstract::C1q is an important component of the classical complement pathway. This study aimed to investigate C1q concentrations in the sera of PM/DM patients. C1q concentrations were measured in the sera of 87 PM/DM patients and 100 healthy subjects. In addition, the association between C1q concentration and laboratory indexes ...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章

    doi:

    authors: Li L,Chen J,Chen S,Liu C,Zhang F,Li Y

    更新日期:2019-03-01 00:00:00

  • Analysis of lead in circumpulpal dentin of deciduous teeth.

    abstract::Absorbed lead continuously adds to the lead deposited in the vascularized circumpulpal dentin of the teeth. Thus, this dental tissue is expected to contain a lead concentration which reflects the integrated lead exposure during the time from completion of tooth formation to tooth extraction or shedding. A method has b...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章

    doi:

    authors: Grandjean P,Hansen ON,Lyngbye K

    更新日期:1984-07-01 00:00:00

  • Determination of plasminogen in human serum or plasma by a fibrometric method.

    abstract::A simple, fast, reproducible, and reliable fibrometer method for determination of plasma or serum plasminogen was developed. Results obtained with this method were compared to those obtained with a Coseinolytic method and a Chromogenic Substrate S-2251 method. For all three procedures, serum or plasma samples were aci...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章

    doi:

    authors: Sarji NM,Liu PI,Teaford MJ,Crook L

    更新日期:1981-09-01 00:00:00

  • Neurological complications of drug abuse.

    abstract::Widespread drug abuse, a comparatively recent medicosocial phenomenon, presents protean clinical patterns and challenging diagnostic problems daily that mimic classical medical syndromes. However, few reports have delineated predominantly neurologic syndromes associated with drug abuse. Five patients were observed ill...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章

    doi:

    authors: Stevens H,Restak R

    更新日期:1976-11-01 00:00:00

  • Effect of experimental hyperhomocysteinemia on cardiac structure and function in the rat.

    abstract::Male Sprague-Dawley rats were subjected for 2 weeks to daily injections of homocysteine (Hcy), which increased plasma Hcy approximately 2-fold. Echocardiography indicated significant increases in left ventricular diastolic (13%) and systolic (31%) dimensions and decreases in posterior wall thickness (diastolic, -17%; ...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章

    doi:

    authors: Walker E,Black J,Parris C,Bryda EC,Cansino S,Hunt L,Chappell J,Wehner P,Studeny M,Wright GL

    更新日期:2004-04-01 00:00:00

  • Ionizing radiation and the conceptus: neurophysiologic effects of prenatal X-radiation on offspring.

    abstract::A brief review of the literature precedes the presentation of a radiation behavioral teratology study. The various types of radiation and the units of measure used in radiation biology are discussed. The concept of the radiation-induced teratogenic "triad" of growth retardation, malformation, and death is presented. A...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章

    doi:

    authors: Jensh RP

    更新日期:1985-05-01 00:00:00

  • Measurement of active ion transport in man. Utilizing intact human erythrocytes.

    abstract::Alterations in ion transport have been implicated in the pathogenesis of several disease states. Methods of studying ion transport, however, are rather tedious, time consuming and not well defined in terms of kinetics and reproducibility. This paper describes a rapid, simple method of examining ion transport in intact...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章

    doi:

    authors: Michels RC,Ober KP,Hennessy JF

    更新日期:1981-03-01 00:00:00

  • Hepatitis C genotype analysis: results in a large veteran population with review of the implications for clinical practice.

    abstract:CONTEXT:Hepatitis C virus (HCV) infects up to 1.8% of the US general population, although the rate is significantly higher in military veterans at 5.4-20%. Early detection and accurate diagnosis are critical as chronic HCV infection can lead to liver cirrhosis and hepatocellular carcinoma. Genotype analysis has both th...

    journal_title:Annals of clinical and laboratory science

    pub_type: 杂志文章

    doi:

    authors: Thomas LB,Foulis PR,Mastorides SM,Djilan YA,Skinner O,Borkowski AA

    更新日期:2012-10-01 00:00:00